SSGCID: Target Page

LpboA.19657.a

Uncharacterized protein

CENTER ID:	LpboA.19657.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19657.a.B1.GE41886	full length		1	340

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.3508
UniProt:	Q04PN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNHLTYKDD KSDKFWNIET SGKSFTVTYG KVGTTGTSQT KTFDNEEKCL KEAEKLLSEK LKKGYVKINF SQKKGKSASP NAEMYSIGDF LNTSEFHETA NVGEKLLNKI SKEDKKIVLT KLCTACESIL IGLTGSEEED YAQRLQAATG LSKAKAVFQR KLATYKNQLK KLKVTKPQNK KLLQEINSEL YNSYIINKKS LDEVCTSIRK MKALTGDDDA QKLIIDHVLG RMEIFLEKEQ KRNFLQILKA YEEILPELGF PQKLVYGQYR VGEGIASLVI NAGLLFNDKY MLDSGLTMIP ASVTYKKLAF FLALYYASVK NREIFLQYAA LSVKLESPKS

NT Sequence

atgaaaaacc acctcactta caaagacgac aagtccgata aattctggaa cattgaaaca tctggaaaat cttttaccgt gacttacggt aaagtcggta ccacaggcac atcgcaaacc aaaactttcg ataacgaaga gaaatgtctg aaggaagcgg agaaattgtt aagcgaaaag ttgaaaaagg gatatgtgaa aattaacttc tcgcaaaaaa aaggaaaaag tgcatctcca aatgctgaga tgtattccat tggcgatttt cttaatacga gcgagtttca tgaaaccgcg aacgttggtg aaaaattgct gaataaaatc tcgaaagagg acaaaaaaat tgttttaacc aagttatgta ccgcttgcga atcgattttg atcggcttga ccggaagcga agaagaagat tatgcccaac gacttcaggc agcaacagga ttgtcaaaag caaaagccgt ttttcaaaga aaattagcaa cttacaaaaa tcaactgaaa aagttaaagg ttacgaagcc ccaaaacaaa aaactgcttc aagagatcaa ttcagaatta tacaattcgt acataatcaa caagaaaagt ctagatgaag tttgcacatc cattagaaaa atgaaagcgc tgactggcga cgacgacgca caaaaactca tcattgatca tgttttgggt cgtatggaga tatttcttga aaaggaacaa aaaagaaatt tcctacagat attgaaagct tatgaggaga tcttgccaga acttggattt ccccaaaaac ttgtctatgg ccaatatcga gtaggcgaag gtatcgcttc tctcgttatc aacgcgggtt tactctttaa cgacaaatat atgcttgatt ccgggcttac catgataccc gcttccgtaa cttacaagaa actcgctttt tttctagccc tttactatgc ctcggtaaaa aatagagaaa tatttctgca atatgccgca ctatcggtta aactagaaag tcccaaaagt tag

Details for LpboA.19657.a.B1.GE41886

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK NHLTYKDDKS DKFWNIETSG KSFTVTYGKV GTTGTSQTKT FDNEEKCLKE AEKLLSEKLK KGYVKINFSQ KKGKSASPNA EMYSIGDFLN TSEFHETANV GEKLLNKISK EDKKIVLTKL CTACESILIG LTGSEEEDYA QRLQAATGLS KAKAVFQRKL ATYKNQLKKL KVTKPQNKKL LQEINSELYN SYIINKKSLD EVCTSIRKMK ALTGDDDAQK LIIDHVLGRM EIFLEKEQKR NFLQILKAYE EILPELGFPQ KLVYGQYRVG EGIASLVINA GLLFNDKYML DSGLTMIPAS VTYKKLAFFL ALYYASVKNR EIFLQYAALX XX

Validated NT Sequence

ttactcactt aacttttggg actttctann nnnnnnntag tgcggcatat tgcagaaata tttctctatt ttttaccgag gcatagtaaa gggctagaaa aaaagcgagt ttcttgtaag ttacggaagc gggtatcatg gtaagcccgg aatcaagcat atatttgtcg ttaaagagta aacccgcgtt gataacgaga gaagcgatac cttcgcctac tcgatattgg ccatagacaa gtttttgggg aaatccaagt tctggcaaga tctcctcata agctttcaat atctgtagga aatttctttt ttgttccttt tcaagaaata tctccatacg acccaaaaca tgatcaatga tgagtttttg tgcgtcgtcg tcgccagtca gcgctttcat ttttctaatg gatgtgcaaa cttcatctag acttttcttg ttgattatgt acgaattgta taattctgaa ttgatctctt gaagcagttt tttgttttgg ggcttcgtaa cctttaactt tttcagttga tttttgtaag ttgctaattt tctttgaaaa acggcttttg cttttgacaa tcctgttgct gcctgaagtc gttgggcata atcttcttct tcgcttccgg tcaagccgat caaaatcgat tcgcaagcgg tacataactt ggttaaaaca atttttttgt cctctttcga gattttattc agcaattttt caccaacgtt cgcggtttca tgaaactcgc tcgtattaag aaaatcgcca atggaataca tctcagcatt tggagatgca ctttttcctt ttttttgcga gaagttaatt ttcacatatc cctttttcaa cttttcgctt aacaatttct ccgcttcctt cagacatttc tcttcgttat cgaaagtttt ggtttgcgat gtgcctgtgg taccgacttt accgtaagtc acggtaaaag attttccaga tgtttcaatg ttccagaatt tatcggactt gtcgtctttg taagtgaggt ggtttttcat atggtggtgg tggtggtgag ccatggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa accacctcac ttacaaagac gacaagtccg ataaattctg gaacattgaa acatctggaa aatcttttac cgtgacttac ggtaaagtcg gtaccacagg cacatcgcaa accaaaactt tcgataacga agagaaatgt ctgaaggaag cggagaaatt gttaagcgaa aagttgaaaa agggatatgt gaaaattaac ttctcgcaaa aaaaaggaaa aagtgcatct ccaaatgctg agatgtattc cattggcgat tttcttaata cgagcgagtt tcatgaaacc gcgaacgttg gtgaaaaatt gctgaataaa atctcgaaag aggacaaaaa aattgtttta accaagttat gtaccgcttg cgaatcgatt ttgatcggct tgaccggaag cgaagaagaa gattatgccc aacgacttca ggcagcaaca ggattgtcaa aagcaaaagc cgtttttcaa agaaaattag caacttacaa aaatcaactg aaaaagttaa aggttacgaa gccccaaaac aaaaaactgc ttcaagagat caattcagaa ttatacaatt cgtacataat caacaagaaa agtctagatg aagtttgcac atccattaga aaaatgaaag cgctgactgg cgacgacgac gcacaaaaac tcatcattga tcatgttttg ggtcgtatgg agatatttct tgaaaaggaa caaaaaagaa atttcctaca gatattgaaa gcttatgagg agatcttgcc agaacttgga tttccccaaa aacttgtcta tggccaatat cgagtaggcg aaggtatcgc ttctctcgtt atcaacgcgg gtttactctt taacgacaaa tatatgcttg attccgggct taccatgata cccgcttccg taacttacaa gaaactcgct ttttttctag ccctttacta tgcctcggta aaaaatagag aaatatttct gcaatatgcc gcactatcgg ttaaactaga aagtcccaaa agttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat