LpboA.19655.b
N-acetylneuraminic acid (Sialic acid) synthetase
| CENTER ID: | LpboA.19655.b |
| ORGANISM: | Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19655.b.B1.GE41883 | full length | 1 | 282 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19655.b.B1.PS38488 | full length | 1 | 282 |
Structures
6NCS
DEPOSITED: 12/12/2018
DETERMINATION: XRay
CLONE: LpboA.19655.b.B1.GE41883
PROTEIN: LpboA.19655.b.B1.PS38488
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|355276.3.peg.1527 |
| UniProt: | Q04TM5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQEITLGNI KIGGNNPVFI IAEAGLNHGG DLNLALRMID EAADAKANAI KFQAYNSEER FGENKEAVNL VKPAEFGKKE FLLLKERSQK KNILFFATPF DVPNLNMLKE IGVEILKIAS CDICNITLLE AAADSGLIVI LSRGTASASE IETAVSIFKK KKSPFILLHC VSSYPMNEID ANLSAIQTLK SKYEFPIGYS DHSKGIEIPL LAVASGAEII EKHYTVDRTL QGIDWEISAE PKELAKLVTE TERIRKILGH GKLEPQASEQ EEIEYRNSLR RK
NT Sequence
atgagtcaag aaattacttt aggaaatatt aaaataggtg gcaataaccc tgtttttatt atagcagaag ctggtttgaa tcatggtgga gatttaaacc ttgccttacg aatgattgat gaggcagcag acgcaaaggc aaatgccata aaatttcaag cttacaacag cgaggaacga tttggtgaaa ataaagaagc tgtgaatttg gtcaaaccgg cagaatttgg aaaaaaagaa tttttattat taaaagaaag atctcaaaag aaaaatatac ttttttttgc tacgccattt gacgttccaa atttaaatat gctcaaagaa atcggggttg aaattttaaa gattgcttct tgcgatattt gtaatataac attattagaa gctgctgccg attcgggttt gattgtgatt ctttctcgtg gaactgcgtc tgcttctgaa atagagacag cagtatctat ttttaagaaa aagaaatccc cttttattct gttgcattgc gtgtcttctt atccaatgaa tgaaatagat gcgaatttat ccgctattca gactttaaaa tctaaatatg aatttccgat tggatactcg gatcatagta agggaattga aatacctttg cttgcagttg caagcggtgc agagattatc gaaaaacatt atactgtgga tcgaactcta cagggaattg attgggaaat ctctgcagag cctaaggaac ttgctaaatt agtgacggag acagagcgca ttcgtaaaat tcttgggcat ggaaagttag aaccccaagc ttctgaacaa gaagaaatag aatatagaaa tagtcttaga agaaagtaa
Details for LpboA.19655.b.B1.GE41883
| HARVESTED ON: | 8/31/2017 |
| SEQUENCED ON: | 9/29/2017 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS QEITLGNIKI GGNNPVFIIA EAGLNHGGDL NLALRMIDEA ADAKANAIKF QAYNSEERFG ENKEAVNLVK PAEFGKKEFL LLKERSQKKN ILFFATPFDV PNLNMLKEIG VEILKIASCD ICNITLLEAA ADSGLIVILS RGTASASEIE TAVSIFKKKK SPFILLHCVS SYPMNEIDAN LSAIQTLKSK YEFPIGYSDH SKGIEIPLLA VASGAEIIEK HYTVDRTLQG IDWEISAEPK ELAKLVTETE RIRKILGHGK LEPQASEQEE IXXXNSLRRK
Validated NT Sequence
ttactcactt actttcttct aagactattt nnnnnntcta tttcttcttg ttcagaagct tggggttcta actttccatg cccaagaatt ttacgaatgc gctctgtctc cgtcactaat ttagcaagtt ccttaggctc tgcagagatt tcccaatcaa ttccctgtag agttcgatcc acagtataat gtttttcgat aatctctgca ccgcttgcaa ctgcaagcaa aggtatttca attcccttac tatgatccga gtatccaatc ggaaattcat atttagattt taaagtctga atagcggata aattcgcatc tatttcattc attggataag aagacacgca atgcaacaga ataaaagggg atttcttttt cttaaaaata gatactgctg tctctatttc agaagcagac gcagttccac gagaaagaat cacaatcaaa cccgaatcgg cagcagcttc taataatgtt atattacaaa tatcgcaaga agcaatcttt aaaatttcaa ccccgatttc tttgagcata tttaaatttg gaacgtcaaa tggcgtagca aaaaaaagta tatttttctt ttgagatctt tcttttaata ataaaaattc tttttttcca aattctgccg gtttgaccaa attcacagct tctttatttt caccaaatcg ttcctcgctg ttgtaagctt gaaattttat ggcatttgcc tttgcgtctg ctgcctcatc aatcattcgt aaggcaaggt ttaaatctcc accatgattc aaaccagctt ctgctataat aaaaacaggg ttattgccac ctattttaat atttcctaaa gtaatttctt gactcatatg gtggtggtgg tggtgagcca tggtat
Expected Protein Sequence
MAHHHHHHMS QEITLGNIKI GGNNPVFIIA EAGLNHGGDL NLALRMIDEA ADAKANAIKF QAYNSEERFG ENKEAVNLVK PAEFGKKEFL LLKERSQKKN ILFFATPFDV PNLNMLKEIG VEILKIASCD ICNITLLEAA ADSGLIVILS RGTASASEIE TAVSIFKKKK SPFILLHCVS SYPMNEIDAN LSAIQTLKSK YEFPIGYSDH SKGIEIPLLA VASGAEIIEK HYTVDRTLQG IDWEISAEPK ELAKLVTETE RIRKILGHGK LEPQASEQEE IEYRNSLRRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc aagaaattac tttaggaaat attaaaatag gtggcaataa ccctgttttt attatagcag aagctggttt gaatcatggt ggagatttaa accttgcctt acgaatgatt gatgaggcag cagacgcaaa ggcaaatgcc ataaaatttc aagcttacaa cagcgaggaa cgatttggtg aaaataaaga agctgtgaat ttggtcaaac cggcagaatt tggaaaaaaa gaatttttat tattaaaaga aagatctcaa aagaaaaata tacttttttt tgctacgcca tttgacgttc caaatttaaa tatgctcaaa gaaatcgggg ttgaaatttt aaagattgct tcttgcgata tttgtaatat aacattatta gaagctgctg ccgattcggg tttgattgtg attctttctc gtggaactgc gtctgcttct gaaatagaga cagcagtatc tatttttaag aaaaagaaat ccccttttat tctgttgcat tgcgtgtctt cttatccaat gaatgaaata gatgcgaatt tatccgctat tcagacttta aaatctaaat atgaatttcc gattggatac tcggatcata gtaagggaat tgaaatacct ttgcttgcag ttgcaagcgg tgcagagatt atcgaaaaac attatactgt ggatcgaact ctacagggaa ttgattggga aatctctgca gagcctaagg aacttgctaa attagtgacg gagacagagc gcattcgtaa aattcttggg catggaaagt tagaacccca agcttctgaa caagaagaaa tagaatatag aaatagtctt agaagaaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LpboA.19655.b.B1.PS38488
| PURIFICATION DATe: | 8/7/2018 |
| CONCENTRATION: | 34.4mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QEITLGNIKI GGNNPVFIIA EAGLNHGGDL NLALRMIDEA ADAKANAIKF QAYNSEERFG ENKEAVNLVK PAEFGKKEFL LLKERSQKKN ILFFATPFDV PNLNMLKEIG VEILKIASCD ICNITLLEAA ADSGLIVILS RGTASASEIE TAVSIFKKKK SPFILLHCVS SYPMNEIDAN LSAIQTLKSK YEFPIGYSDH SKGIEIPLLA VASGAEIIEK HYTVDRTLQG IDWEISAEPK ELAKLVTETE RIRKILGHGK LEPQASEQEE IXXXNSLRRK
Validated NT Sequence
ttactcactt actttcttct aagactattt nnnnnntcta tttcttcttg ttcagaagct tggggttcta actttccatg cccaagaatt ttacgaatgc gctctgtctc cgtcactaat ttagcaagtt ccttaggctc tgcagagatt tcccaatcaa ttccctgtag agttcgatcc acagtataat gtttttcgat aatctctgca ccgcttgcaa ctgcaagcaa aggtatttca attcccttac tatgatccga gtatccaatc ggaaattcat atttagattt taaagtctga atagcggata aattcgcatc tatttcattc attggataag aagacacgca atgcaacaga ataaaagggg atttcttttt cttaaaaata gatactgctg tctctatttc agaagcagac gcagttccac gagaaagaat cacaatcaaa cccgaatcgg cagcagcttc taataatgtt atattacaaa tatcgcaaga agcaatcttt aaaatttcaa ccccgatttc tttgagcata tttaaatttg gaacgtcaaa tggcgtagca aaaaaaagta tatttttctt ttgagatctt tcttttaata ataaaaattc tttttttcca aattctgccg gtttgaccaa attcacagct tctttatttt caccaaatcg ttcctcgctg ttgtaagctt gaaattttat ggcatttgcc tttgcgtctg ctgcctcatc aatcattcgt aaggcaaggt ttaaatctcc accatgattc aaaccagctt ctgctataat aaaaacaggg ttattgccac ctattttaat atttcctaaa gtaatttctt gactcatatg gtggtggtgg tggtgagcca tggtat
Expressed Protein Sequence
MAHHHHHHMS QEITLGNIKI GGNNPVFIIA EAGLNHGGDL NLALRMIDEA ADAKANAIKF QAYNSEERFG ENKEAVNLVK PAEFGKKEFL LLKERSQKKN ILFFATPFDV PNLNMLKEIG VEILKIASCD ICNITLLEAA ADSGLIVILS RGTASASEIE TAVSIFKKKK SPFILLHCVS SYPMNEIDAN LSAIQTLKSK YEFPIGYSDH SKGIEIPLLA VASGAEIIEK HYTVDRTLQG IDWEISAEPK ELAKLVTETE RIRKILGHGK LEPQASEQEE IEYRNSLRRK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC AAGAAATTAC TTTAGGAAAT ATTAAAATAG GTGGCAATAA CCCTGTTTTT ATTATAGCAG AAGCTGGTTT GAATCATGGT GGAGATTTAA ACCTTGCCTT ACGAATGATT GATGAGGCAG CAGACGCAAA GGCAAATGCC ATAAAATTTC AAGCTTACAA CAGCGAGGAA CGATTTGGTG AAAATAAAGA AGCTGTGAAT TTGGTCAAAC CGGCAGAATT TGGAAAAAAA GAATTTTTAT TATTAAAAGA AAGATCTCAA AAGAAAAATA TACTTTTTTT TGCTACGCCA TTTGACGTTC CAAATTTAAA TATGCTCAAA GAAATCGGGG TTGAAATTTT AAAGATTGCT TCTTGCGATA TTTGTAATAT AACATTATTA GAAGCTGCTG CCGATTCGGG TTTGATTGTG ATTCTTTCTC GTGGAACTGC GTCTGCTTCT GAAATAGAGA CAGCAGTATC TATTTTTAAG AAAAAGAAAT CCCCTTTTAT TCTGTTGCAT TGCGTGTCTT CTTATCCAAT GAATGAAATA GATGCGAATT TATCCGCTAT TCAGACTTTA AAATCTAAAT ATGAATTTCC GATTGGATAC TCGGATCATA GTAAGGGAAT TGAAATACCT TTGCTTGCAG TTGCAAGCGG TGCAGAGATT ATCGAAAAAC ATTATACTGT GGATCGAACT CTACAGGGAA TTGATTGGGA AATCTCTGCA GAGCCTAAGG AACTTGCTAA ATTAGTGACG GAGACAGAGC GCATTCGTAA AATTCTTGGG CATGGAAAGT TAGAACCCCA AGCTTCTGAA CAAGAAGAAA TAGAATATAG AAATAGTCTT AGAAGAAAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT