LpboA.19655.a

N-acetylneuraminic acid (Sialic acid) synthetase

CENTER ID: LpboA.19655.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19655.a.B1.GE41882 full length 1 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.1446
UniProt: Q04TP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFYSLRVHC MPQPIQLGNK IIAGFFDPYV IAEIGVNHEG SIDIAKRLID EAKDGGADGA KFQTYKAETL ASKNSPSYWD RTQEPTASQF ELFKKYDSFG WKEYEILAKH CQSVGIDFLS TPFDLEAVEH LASQMPFIKV ASADITSVPL LRSVAKYSKP VILSTGSSTI PEIDFAMREL HANGANEIIL LHCILNYPTK PENANLGMIQ DLQRIFPHHW VGYSDHTPPD LEMKSLITAT LFGSVVLEKH FTHDKTLPGN DHYHAMDKED LKRFKRKLKE FESLIGSSEK APLQSEELSR KNARRSIVLS KNLSKGSKIK EQDITFKRPA HGISPIFWDD VIGRVINRDL LEDHILNWED LVQL
NT Sequence
ttgatctttt atagcttaag ggtgcattgt atgccacaac cgattcaatt gggaaataaa attattgctg gttttttcga tccttatgtg atcgctgaga taggggtcaa tcatgaagga tcgatcgata tagcgaaacg attgattgac gaagccaaag acggtggggc ggacggagca aaatttcaaa cctataaagc ggagacgtta gcatctaaga attcaccatc ctattgggat agaactcaag agcccaccgc tagtcaattt gaattattta aaaaatatga ttctttcggt tggaaagagt atgaaatact cgccaaacat tgccaatcgg tcgggattga ttttctttcg acccctttcg atctggaggc ggtggaacat ttggcttctc aaatgccttt tataaaagtc gcttcggcgg atataacttc tgttcctttg ttaagaagcg ttgctaaata ttctaagcca gtaattttat caactggtag ttcgactatt ccagagattg attttgcaat gagagagttg catgcaaatg gagcaaacga gattattctt ttgcattgta tattaaatta tcccactaaa cctgaaaatg caaatttggg tatgatccag gacttacaaa gaatatttcc gcaccactgg gttggatatt cagaccacac tcctccagat cttgagatga aatctttgat aaccgctacc ttgtttggtt ccgtcgtttt ggaaaaacat tttactcatg acaaaactct ccctggaaat gatcattatc atgcgatgga taaggaagat cttaaaagat tcaaaagaaa acttaaggaa tttgaatctt taatcggcag tagtgaaaaa gcaccgcttc aatctgaaga actgagccgg aaaaacgcaa gaagatctat tgtcctttct aaaaatcttt cgaaaggtag caaaatcaaa gaacaagata taacttttaa aaggcctgcg catgggatta gccctatttt ttgggatgat gtaattggaa gagtgatcaa tagagatctt ttagaagacc atattttaaa ttgggaagat ttggttcaac tttag
Details for LpboA.19655.a.B1.GE41882
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML IFYSLRVHCM PQPIQLGNKI IAGFFDPYVI AEIGVNHEGS IDIAKRLIDE AKDGGADGAK FQTYKAETLA SKNSPSYWDR TQEPTASQFE LFKKYDSFGW KEYEILAKHC QSVGIDFLST PFDLEAVEHL ASQMPFIKVA SADITSVPLL RSVAKYSKPV ILSTGSSTIP EIDFAMRELH ANGANEIILL HCILNYPTKP ENANLGMIQD LQRIFPHHWV GYSDHTPPDL EMKSLITATL FGSVVLEKHF THDKTLPGND HYHAMDKEDL KRFKRKLKEF ESLIGSSEKA PLQSEELSRK NARRSIVLSK NLSKGSKIKE QDITFKRPAH GISPIFWDDV IGRVINRDLL EDHI
Validated NT Sequence
taaaatatgg tcttctaaaa gatctctatt gatcactctt ccaattacat catcccaaaa aatagggcta atcccatgcg caggcctttt aaaagttata tcttgttctt tgattttgct acctttcgaa agatttttag aaaggacaat agatcttctt gcgtttttcc ggctcagttc ttcagattga agcggtgctt tttcactact gccgattaaa gattcaaatt ccttaagttt tcttttgaat cttttaagat cttccttatc catcgcatga taatgatcat ttccagggag agttttgtca tgagtaaaat gtttttccaa aacgacggaa ccaaacaagg tagcggttat caaagatttc atctcaagat ctggaggagt gtggtctgaa tatccaaccc agtggtgcgg aaatattctt tgtaagtcct ggatcatacc caaatttgca ttttcaggtt tagtgggata atttaatata caatgcaaaa gaataatctc gtttgctcca tttgcatgca actctctcat tgcaaaatca atctctggaa tagtcgaact accagttgat aaaattactg gcttagaata tttagcaacg cttcttaaca aaggaacaga agttatatcc gccgaagcga cttttataaa aggcatttga gaagccaaat gttccaccgc ctccagatcg aaaggggtcg aaagaaaatc aatcccgacc gattggcaat gtttggcgag tatttcatac tctttccaac cgaaagaatc atatttttta aataattcaa attgactagc ggtgggctct tgagttctat cccaatagga tggtgaattc ttagatgcta acgtctccgc tttataggtt tgaaattttg ctccgtccgc cccaccgtct ttggcttcgt caatcaatcg tttcgctata tcgatcgatc cttcatgatt gacccctatc tcagcgatca cataaggatc gaaaaaacca gcaataattt tatttcccaa ttgaatcggt tgtggcatac aatgcaccct taagctataa aagatcaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMI FYSLRVHCMP QPIQLGNKII AGFFDPYVIA EIGVNHEGSI DIAKRLIDEA KDGGADGAKF QTYKAETLAS KNSPSYWDRT QEPTASQFEL FKKYDSFGWK EYEILAKHCQ SVGIDFLSTP FDLEAVEHLA SQMPFIKVAS ADITSVPLLR SVAKYSKPVI LSTGSSTIPE IDFAMRELHA NGANEIILLH CILNYPTKPE NANLGMIQDL QRIFPHHWVG YSDHTPPDLE MKSLITATLF GSVVLEKHFT HDKTLPGNDH YHAMDKEDLK RFKRKLKEFE SLIGSSEKAP LQSEELSRKN ARRSIVLSKN LSKGSKIKEQ DITFKRPAHG ISPIFWDDVI GRVINRDLLE DHILNWEDLV QL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgatct tttatagctt aagggtgcat tgtatgccac aaccgattca attgggaaat aaaattattg ctggtttttt cgatccttat gtgatcgctg agataggggt caatcatgaa ggatcgatcg atatagcgaa acgattgatt gacgaagcca aagacggtgg ggcggacgga gcaaaatttc aaacctataa agcggagacg ttagcatcta agaattcacc atcctattgg gatagaactc aagagcccac cgctagtcaa tttgaattat ttaaaaaata tgattctttc ggttggaaag agtatgaaat actcgccaaa cattgccaat cggtcgggat tgattttctt tcgacccctt tcgatctgga ggcggtggaa catttggctt ctcaaatgcc ttttataaaa gtcgcttcgg cggatataac ttctgttcct ttgttaagaa gcgttgctaa atattctaag ccagtaattt tatcaactgg tagttcgact attccagaga ttgattttgc aatgagagag ttgcatgcaa atggagcaaa cgagattatt cttttgcatt gtatattaaa ttatcccact aaacctgaaa atgcaaattt gggtatgatc caggacttac aaagaatatt tccgcaccac tgggttggat attcagacca cactcctcca gatcttgaga tgaaatcttt gataaccgct accttgtttg gttccgtcgt tttggaaaaa cattttactc atgacaaaac tctccctgga aatgatcatt atcatgcgat ggataaggaa gatcttaaaa gattcaaaag aaaacttaag gaatttgaat ctttaatcgg cagtagtgaa aaagcaccgc ttcaatctga agaactgagc cggaaaaacg caagaagatc tattgtcctt tctaaaaatc tttcgaaagg tagcaaaatc aaagaacaag atataacttt taaaaggcct gcgcatggga ttagccctat tttttgggat gatgtaattg gaagagtgat caatagagat cttttagaag accatatttt aaattgggaa gatttggttc aactttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat