LpboA.19561.b
Uncharacterized protein
| CENTER ID: | LpboA.19561.b |
| ORGANISM: | Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19561.b.B1.GE41881 | full length | 1 | 222 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19561.b.B1.PS38498 | full length | 1 | 222 |
Structures
6OHZ
DEPOSITED: 4/8/2019
DETERMINATION: XRay
CLONE: LpboA.19561.b.B1.GE41881
PROTEIN: LpboA.19561.b.B1.PS38498
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | Q04PE5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEIDDLLRK NPELQKEWKR TVWTAAISSG VIAYRPPLLE RAFREFPMET AKSALNLFVA AHKSKNRQSV DIITQNLKDA KTFPLGQLEE EIVTDILKYP NLLEKLLQTG WNPNLILEWE KHKSLSQNSK RSHRRPEILI KSNGKEFIEK QETTLLILAM QNDFIPMETV QILLKYGADP SLGVKRKSEG KEYLLYPLAN INSNGNTILK ELKQKTLIDW KK
NT Sequence
ctgacggaaa tcgacgacct tcttcgaaaa aatccggaac tgcaaaaaga atggaaacgc acggtgtgga cggccgccat ttcttccgga gttatcgcct atcgccctcc attactggaa agagcgtttc gagagtttcc aatggaaacc gcaaagtcgg ccctaaattt attcgtggcc gcacacaaaa gtaaaaatcg ccaatccgta gatatcatta ctcaaaatct taaggatgcc aaaacatttc ctttgggcca gttggaagaa gaaatcgtaa cagacatatt aaaatatcct aatttattag agaaattact tcagaccggt tggaatccta atttaatttt ggaatgggaa aaacataaga gtctgtccca aaactcaaaa cgatcacacc gacgcccaga gattctgata aaatcgaatg gaaaagaatt cattgaaaaa caagaaacaa ctcttctcat ccttgcgatg caaaacgatt ttattccgat ggaaacggtt caaattttat tgaaatacgg agccgatcct agtttaggag taaaacgtaa gtccgaagga aaagaatatc tactctatcc tttggcaaac atcaattcaa acggaaatac aattctcaaa gaactcaaac agaaaactct aatagattgg aaaaaataa
Details for LpboA.19561.b.B1.GE41881
| HARVESTED ON: | 8/31/2017 |
| SEQUENCED ON: | 9/29/2017 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM LTEIDDLLRK NPELQKEWKR TVWTAAISSG VIAYRPPLLE RAFREFPMET AKSALNLFVA AHKSKNRQSV DIITQNLKDA KTFPLGQLEE EIVTDILKYP NLLEKLLQTG WNPNLILEWE KHKSLSQNSK RSHRRPEILI KSNGKEFIEK QETTLLILAM QNDFIPMETV QILLKYGADP SLGVKRKSEG KEYLLYPLAN INSNGNTILK ELKQKTLIDW KK
Validated NT Sequence
atcttactca cttatttttt ccaatctatt agagttttct gtttgagttc tttgagaatt gtatttccgt ttgaattgat gtttgccaaa ggatagagta gatattcttt tccttcggac ttacgtttta ctcctaaact aggatcggct ccgtatttca ataaaatttg aaccgtttcc atcggaataa aatcgttttg catcgcaagg atgagaagag ttgtttcttg tttttcaatg aattcttttc cattcgattt tatcagaatc tctgggcgtc ggtgtgatcg ttttgagttt tgggacagac tcttatgttt ttcccattcc aaaattaaat taggattcca accggtctga agtaatttct ctaataaatt aggatatttt aatatgtctg ttacgatttc ttcttccaac tggcccaaag gaaatgtttt ggcatcctta agattttgag taatgatatc tacggattgg cgatttttac ttttgtgtgc ggccacgaat aaatttaggg ccgactttgc ggtttccatt ggaaactctc gaaacgctct ttccagtaat ggagggcgat aggcgataac tccggaagaa atggcggccg tccacaccgt gcgtttccat tctttttgca gttccggatt ttttcgaaga aggtcgtcga tttccgtcag catatggtgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT EIDDLLRKNP ELQKEWKRTV WTAAISSGVI AYRPPLLERA FREFPMETAK SALNLFVAAH KSKNRQSVDI ITQNLKDAKT FPLGQLEEEI VTDILKYPNL LEKLLQTGWN PNLILEWEKH KSLSQNSKRS HRRPEILIKS NGKEFIEKQE TTLLILAMQN DFIPMETVQI LLKYGADPSL GVKRKSEGKE YLLYPLANIN SNGNTILKEL KQKTLIDWKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgacgg aaatcgacga ccttcttcga aaaaatccgg aactgcaaaa agaatggaaa cgcacggtgt ggacggccgc catttcttcc ggagttatcg cctatcgccc tccattactg gaaagagcgt ttcgagagtt tccaatggaa accgcaaagt cggccctaaa tttattcgtg gccgcacaca aaagtaaaaa tcgccaatcc gtagatatca ttactcaaaa tcttaaggat gccaaaacat ttcctttggg ccagttggaa gaagaaatcg taacagacat attaaaatat cctaatttat tagagaaatt acttcagacc ggttggaatc ctaatttaat tttggaatgg gaaaaacata agagtctgtc ccaaaactca aaacgatcac accgacgccc agagattctg ataaaatcga atggaaaaga attcattgaa aaacaagaaa caactcttct catccttgcg atgcaaaacg attttattcc gatggaaacg gttcaaattt tattgaaata cggagccgat cctagtttag gagtaaaacg taagtccgaa ggaaaagaat atctactcta tcctttggca aacatcaatt caaacggaaa tacaattctc aaagaactca aacagaaaac tctaatagat tggaaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LpboA.19561.b.B1.PS38498
| PURIFICATION DATe: | 9/5/2018 |
| CONCENTRATION: | 33.72mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 20µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM LTEIDDLLRK NPELQKEWKR TVWTAAISSG VIAYRPPLLE RAFREFPMET AKSALNLFVA AHKSKNRQSV DIITQNLKDA KTFPLGQLEE EIVTDILKYP NLLEKLLQTG WNPNLILEWE KHKSLSQNSK RSHRRPEILI KSNGKEFIEK QETTLLILAM QNDFIPMETV QILLKYGADP SLGVKRKSEG KEYLLYPLAN INSNGNTILK ELKQKTLIDW KK
Validated NT Sequence
atcttactca cttatttttt ccaatctatt agagttttct gtttgagttc tttgagaatt gtatttccgt ttgaattgat gtttgccaaa ggatagagta gatattcttt tccttcggac ttacgtttta ctcctaaact aggatcggct ccgtatttca ataaaatttg aaccgtttcc atcggaataa aatcgttttg catcgcaagg atgagaagag ttgtttcttg tttttcaatg aattcttttc cattcgattt tatcagaatc tctgggcgtc ggtgtgatcg ttttgagttt tgggacagac tcttatgttt ttcccattcc aaaattaaat taggattcca accggtctga agtaatttct ctaataaatt aggatatttt aatatgtctg ttacgatttc ttcttccaac tggcccaaag gaaatgtttt ggcatcctta agattttgag taatgatatc tacggattgg cgatttttac ttttgtgtgc ggccacgaat aaatttaggg ccgactttgc ggtttccatt ggaaactctc gaaacgctct ttccagtaat ggagggcgat aggcgataac tccggaagaa atggcggccg tccacaccgt gcgtttccat tctttttgca gttccggatt ttttcgaaga aggtcgtcga tttccgtcag catatggtgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMT EIDDLLRKNP ELQKEWKRTV WTAAISSGVI AYRPPLLERA FREFPMETAK SALNLFVAAH KSKNRQSVDI ITQNLKDAKT FPLGQLEEEI VTDILKYPNL LEKLLQTGWN PNLILEWEKH KSLSQNSKRS HRRPEILIKS NGKEFIEKQE TTLLILAMQN DFIPMETVQI LLKYGADPSL GVKRKSEGKE YLLYPLANIN SNGNTILKEL KQKTLIDWKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGACGG AAATCGACGA CCTTCTTCGA AAAAATCCGG AACTGCAAAA AGAATGGAAA CGCACGGTGT GGACGGCCGC CATTTCTTCC GGAGTTATCG CCTATCGCCC TCCATTACTG GAAAGAGCGT TTCGAGAGTT TCCAATGGAA ACCGCAAAGT CGGCCCTAAA TTTATTCGTG GCCGCACACA AAAGTAAAAA TCGCCAATCC GTAGATATCA TTACTCAAAA TCTTAAGGAT GCCAAAACAT TTCCTTTGGG CCAGTTGGAA GAAGAAATCG TAACAGACAT ATTAAAATAT CCTAATTTAT TAGAGAAATT ACTTCAGACC GGTTGGAATC CTAATTTAAT TTTGGAATGG GAAAAACATA AGAGTCTGTC CCAAAACTCA AAACGATCAC ACCGACGCCC AGAGATTCTG ATAAAATCGA ATGGAAAAGA ATTCATTGAA AAACAAGAAA CAACTCTTCT CATCCTTGCG ATGCAAAACG ATTTTATTCC GATGGAAACG GTTCAAATTT TATTGAAATA CGGAGCCGAT CCTAGTTTAG GAGTAAAACG TAAGTCCGAA GGAAAAGAAT ATCTACTCTA TCCTTTGGCA AACATCAATT CAAACGGAAA TACAATTCTC AAAGAACTCA AACAGAAAAC TCTAATAGAT TGGAAAAAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT