LpboA.19559.a

TPR-repeat protein

CENTER ID: LpboA.19559.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19559.a.B2.GE41879 soluble domain 45 220
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.2676
UniProt: Q04R79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNNFEGITK DLKIKSIMRL KKQAHHDSIL FFIISISLIV ALNACNKDEQ SKLETFQKGK KEYTSRNLEK AADFFRQVAS EGKEFLPAQI MLGKSLFFLG KMEESEEIFH KGLTRFPGNS TIRFWIARIH MLQEGKTKEA KQELEYILET EETFFAAHYY LAKIYEKEGM LKEALIEYNR AKMIKIGFDK IHRDLGKLYD AAGLHEKAAL EKDLLFEKAE
NT Sequence
atgaaaaata attttgaagg aatcaccaag gatctgaaaa tcaaatcgat aatgcgactt aaaaaacaag cgcatcacga ctcgatttta ttctttataa tttccatttc tcttatcgtc gctttaaacg cgtgcaacaa ggatgaacaa tccaaattgg aaacctttca aaaaggaaaa aaggaataca cttcccgaaa tttagagaag gccgccgact tttttcgaca agtcgcttcg gagggtaaag aatttttacc ggctcaaatt atgttaggta agagcctctt tttcttggga aaaatggagg aatcggagga aatatttcac aaaggattaa ctcgcttccc gggaaactcg acgattcgat tttggatagc aaggatccac atgttgcagg aaggcaagac gaaagaagcg aagcaggaac ttgaatacat tttagaaacg gaagaaactt ttttcgctgc acactattat ttggcaaaaa tatatgaaaa agaaggaatg ttgaaggagg ccttgattga atacaaccgc gccaagatga tcaaaattgg ttttgataaa atccatagag atctgggtaa actatacgat gctgcaggtt tacacgaaaa agcggcttta gaaaaagacc tacttttcga aaaagcagaa tga
Details for LpboA.19559.a.B2.GE41879
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 22kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC NKDEQSKLET FQKGKKEYTS RNLEKAADFF RQVASEGKEF LPAQIMLGKS LFFLGKMEES EEIFHKGLTR FPGNSTIRFW IARIHMLQEG KTKEAKQELE YILETEETFF AAHYYLAKIY EKEGMLKEAL IEYNRAKMIK IGFDKIHRDL GKLYDAAGLH EKAALEKDLL FEKAE
Validated NT Sequence
atcttactca cttattctgc tttttcgaaa agtaggtctt tttctaaagc cgctttttcg tgtaaacctg cagcatcgta tagtttaccc agatctctat ggattttatc aaaaccaatt ttgatcatct tggcgcggtt gtattcaatc aaggcctcct tcaacattcc ttctttttca tatatttttg ccaaataata gtgtgcagcg aaaaaagttt cttccgtttc taaaatgtat tcaagttcct gcttcgcttc tttcgtcttg ccttcctgca acatgtggat ccttgctatc caaaatcgaa tcgtcgagtt tcccgggaag cgagttaatc ctttgtgaaa tatttcctcc gattcctcca tttttcccaa gaaaaagagg ctcttaccta acataatttg agccggtaaa aattctttac cctccgaagc gacttgtcga aaaaagtcgg cggccttctc taaatttcgg gaagtgtatt ccttttttcc tttttgaaag gtttccaatt tggattgttc atccttgttg cacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHCN KDEQSKLETF QKGKKEYTSR NLEKAADFFR QVASEGKEFL PAQIMLGKSL FFLGKMEESE EIFHKGLTRF PGNSTIRFWI ARIHMLQEGK TKEAKQELEY ILETEETFFA AHYYLAKIYE KEGMLKEALI EYNRAKMIKI GFDKIHRDLG KLYDAAGLHE KAALEKDLLF EKAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgcaaca aggatgaaca atccaaattg gaaacctttc aaaaaggaaa aaaggaatac acttcccgaa atttagagaa ggccgccgac ttttttcgac aagtcgcttc ggagggtaaa gaatttttac cggctcaaat tatgttaggt aagagcctct ttttcttggg aaaaatggag gaatcggagg aaatatttca caaaggatta actcgcttcc cgggaaactc gacgattcga ttttggatag caaggatcca catgttgcag gaaggcaaga cgaaagaagc gaagcaggaa cttgaataca ttttagaaac ggaagaaact tttttcgctg cacactatta tttggcaaaa atatatgaaa aagaaggaat gttgaaggag gccttgattg aatacaaccg cgccaagatg atcaaaattg gttttgataa aatccataga gatctgggta aactatacga tgctgcaggt ttacacgaaa aagcggcttt agaaaaagac ctacttttcg aaaaagcaga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat