LpboA.19558.a

Uncharacterized protein

CENTER ID: LpboA.19558.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19558.a.B1.GE41878 full length 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.3293
UniProt: Q04Q47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSKKLYILS FVKEGRPYKG AVNSKGEVII PPNFKQIDYT SDQTELIPAG IPKPNAEHAM DLDWGYINQI GEWKIKPQFD KLTNFYNEWA FAKSKKGQKW GKIDKTGAWI ENPTYQEIGL FSDGLVWAKK DTGQYVFLDE TGKIKFTLDG FDQLSLSFSE KFLGFEKQGK WRFIDTNGAV VLEPKWEYVG HFSKGLAPVY KASAWQYIDT TGQAKLPEPL EEAMEFDISG YAAAKKGRWG VIDMLGNWTI KPQSQVLLAP ENNVITTKLK GEWCTLNMQG EVIEKIPVTK KMEGITPFDE NDVAVAFYGD YAQLLNRQYD VIFDSKKYKA K
NT Sequence
atgaattcga aaaaattata cattttaagt tttgttaaag aaggtcggcc ttacaaaggt gccgttaatt ccaaaggtga agtcataatc ccgccaaatt ttaaacaaat cgattataca agcgatcaaa cggaacttat ccctgcggga attccaaagc ctaatgccga acatgctatg gacttagatt ggggttatat caaccaaatc ggagaatgga aaatcaaacc acagttcgac aaactcacaa atttttataa tgaatgggcc tttgccaaat caaagaaagg gcaaaaatgg ggaaaaatcg ataaaaccgg agcatggata gaaaatccga cgtatcagga aatcggcttg ttttccgacg gcttggtttg ggcaaaaaaa gataccggtc agtacgtttt tctggatgag accgggaaaa ttaaatttac attggacggt tttgatcaac taagtctttc cttttcggaa aaatttctcg gtttcgaaaa acaaggcaaa tggagattta tcgatacaaa cggcgcggta gttttagaac caaaatggga atatgtcggg cattttagta aaggtcttgc gccggtctac aaagcttctg catggcaata tatcgatacg acaggtcagg caaaacttcc cgaaccttta gaagaagcaa tggagtttga catctcaggt tatgccgcgg caaagaaagg ccgatggggg gttattgata tgttgggcaa ttggacaatc aaaccacagt ctcaggtttt gttggcgcca gagaacaatg tcatcactac aaagctcaag ggagaatggt gtacattgaa tatgcagggt gaagtgattg agaaaattcc tgtaactaaa aaaatggaag ggataactcc atttgacgaa aacgacgttg ccgtggcctt ttatggagat tatgctcaac ttctaaatcg gcaatacgac gtaatttttg atagtaaaaa atataaagcg aaataa
Details for LpboA.19558.a.B1.GE41878
HARVESTED ON: data unavailable
SEQUENCED ON: 9/29/2017
EXPECTED MW: 39kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN SKKLYILSFV KEGRPYKGAV NSKGEVIIPP NFKQIDYTSD QTELIPAGIP KPNAEHAMDL DWGYINQIGE WKIKPQFDKL TNFYNEWAFA KSKKGQKWGK IDKTGAWIEN PTYQEIGLFS DGLVWAKKDT GQYVFLDETG KIKFTLDGFD QLSLSFSEKF LGFEKQGKWR FIDTNGAVVL EPKWEYVGHF SKGLAPVYKA SAWQYIDTTG QAKLPEPLEE AMEFDISGYA AAKKGRWGVI DMLGNWTIKP QSQVLLAPEN NVITTKLKGE WCTLNMQGEV IEKIPVTKKM EGITPFDEND VAVAFYGDYA QLLNRQYDVI F
Validated NT Sequence
atcaaaaatt acgtcgtatt gccgatttag aagttgagca taatctccat aaaaggccac ggcaacgtcg ttttcgtcaa atggagttat cccttccatt tttttagtta caggaatttt ctcaatcact tcaccctgca tattcaatgt acaccattct cccttgagct ttgtagtgat gacattgttc tctggcgcca acaaaacctg agactgtggt ttgattgtcc aattgcccaa catatcaata accccccatc ggcctttctt tgccgcggca taacctgaga tgtcaaactc cattgcttct tctaaaggtt cgggaagttt tgcctgacct gtcgtatcga tatattgcca tgcagaagct ttgtagaccg gcgcaagacc tttactaaaa tgcccgacat attcccattt tggttctaaa actaccgcgc cgtttgtatc gataaatctc catttgcctt gtttttcgaa accgagaaat ttttccgaaa aggaaagact tagttgatca aaaccgtcca atgtaaattt aattttcccg gtctcatcca gaaaaacgta ctgaccggta tctttttttg cccaaaccaa gccgtcggaa aacaagccga tttcctgata cgtcggattt tctatccatg ctccggtttt atcgattttt ccccattttt gccctttctt tgatttggca aaggcccatt cattataaaa atttgtgagt ttgtcgaact gtggtttgat tttccattct ccgatttggt tgatataacc ccaatctaag tccatagcat gttcggcatt aggctttgga attcccgcag ggataagttc cgtttgatcg cttgtataat cgatttgttt aaaatttggc gggattatga cttcaccttt ggaattaacg gcacctttgt aaggccgacc ttctttaaca aaacttaaaa tgtataattt tttcgaattc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN SKKLYILSFV KEGRPYKGAV NSKGEVIIPP NFKQIDYTSD QTELIPAGIP KPNAEHAMDL DWGYINQIGE WKIKPQFDKL TNFYNEWAFA KSKKGQKWGK IDKTGAWIEN PTYQEIGLFS DGLVWAKKDT GQYVFLDETG KIKFTLDGFD QLSLSFSEKF LGFEKQGKWR FIDTNGAVVL EPKWEYVGHF SKGLAPVYKA SAWQYIDTTG QAKLPEPLEE AMEFDISGYA AAKKGRWGVI DMLGNWTIKP QSQVLLAPEN NVITTKLKGE WCTLNMQGEV IEKIPVTKKM EGITPFDEND VAVAFYGDYA QLLNRQYDVI FDSKKYKAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt cgaaaaaatt atacatttta agttttgtta aagaaggtcg gccttacaaa ggtgccgtta attccaaagg tgaagtcata atcccgccaa attttaaaca aatcgattat acaagcgatc aaacggaact tatccctgcg ggaattccaa agcctaatgc cgaacatgct atggacttag attggggtta tatcaaccaa atcggagaat ggaaaatcaa accacagttc gacaaactca caaattttta taatgaatgg gcctttgcca aatcaaagaa agggcaaaaa tggggaaaaa tcgataaaac cggagcatgg atagaaaatc cgacgtatca ggaaatcggc ttgttttccg acggcttggt ttgggcaaaa aaagataccg gtcagtacgt ttttctggat gagaccggga aaattaaatt tacattggac ggttttgatc aactaagtct ttccttttcg gaaaaatttc tcggtttcga aaaacaaggc aaatggagat ttatcgatac aaacggcgcg gtagttttag aaccaaaatg ggaatatgtc gggcatttta gtaaaggtct tgcgccggtc tacaaagctt ctgcatggca atatatcgat acgacaggtc aggcaaaact tcccgaacct ttagaagaag caatggagtt tgacatctca ggttatgccg cggcaaagaa aggccgatgg ggggttattg atatgttggg caattggaca atcaaaccac agtctcaggt tttgttggcg ccagagaaca atgtcatcac tacaaagctc aagggagaat ggtgtacatt gaatatgcag ggtgaagtga ttgagaaaat tcctgtaact aaaaaaatgg aagggataac tccatttgac gaaaacgacg ttgccgtggc cttttatgga gattatgctc aacttctaaa tcggcaatac gacgtaattt ttgatagtaa aaaatataaa gcgaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t