LpboA.19557.b

Dehydrogenase

CENTER ID: LpboA.19557.b
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19557.b.B1.GE41876 full length 1 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.1451
UniProt: Q04TN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLGGKPVLQ FLVERLKRSK FIDEIIIATS SNIENDAIES LTKEIGIYCF RGSEGDVLSR VIGAADSRKA DIIVQLTGDC PLMDPELIDE CVSKYLDGGY DYVANELVRT YPIGMDVAVF SLSLLKETYL EKDLTEMDKE HVTTYIVERP IRYKLLNVRA DKEQDRPDLQ LTLDTKEDYS TLSFIVDSLY TKKSDFNLRD IIQFLDQHKE ISAINSEIKR KKKDIRKLSV GLIGLGKIAS DYDSGIDGIE INSHTKAFLK NEFAEIVVVA DQDISKIQNF KMKWDLEPIS FIGLDAFWAS EINLDLLSIC SSTASHFEIL KKAMERNIPY ILCEKPITDK EDELNEIAEL SKKSNSKIIV NHILRWEPGV DSIKEKLSQE KIQKVIVFYA KGFLHNGIHV VDLSLYLFGN PKKIIKRKEH IDGFGYLNFD FDLVYDSFEI SFFALDEEKF SIFEYSIYTD LGKFVIDELT SKIKYFSIIK HPNIEGYQYI GSKAEEIECL QFQSMKNVID DVILGIRTGD ESYFKSDLKA TLSTMNILFD IMRTPCQN
NT Sequence
ttgcctttag gcgggaaacc tgttcttcaa tttttggtcg aaagactcaa acgttccaag tttatagatg aaatcataat tgcgacctct tctaatattg aaaatgatgc aattgaaagt ttgactaagg agatcgggat ttattgtttt cgtggaagtg agggcgatgt tttgagtcgt gtgatcggag cagcggattc taggaaagct gatattatcg ttcaattgac aggagattgt cctttgatgg accccgagtt gattgatgaa tgcgtgagta aatatttaga tggtggttac gattacgtcg caaacgaatt agtgcggact tatccgattg gaatggatgt tgctgttttt tccttatcct tattaaaaga aacatatttg gaaaaggatc ttacggagat ggacaaagag cacgtgacta cttatatagt ggaacggccg attcgttata aattgctgaa tgttcgagca gataaagaac aggaccgtcc ggaccttcaa ctgactttgg atacgaaaga agattattct acattgagtt ttattgttga ttctttatat acgaaaaaaa gtgattttaa tctaagagat ataattcaat ttttagatca gcacaaagag atttctgcaa tcaattcaga aatcaaaaga aaaaagaaag atatccgtaa gttaagtgtt ggtctcattg gccttggaaa aatagccagt gattatgatt ctggtatcga cggcatcgag atcaatagtc atacgaaagc atttcttaaa aatgaatttg cggaaatcgt ggtggttgca gatcaggata tatcgaaaat tcaaaatttt aaaatgaaat gggacttaga accgatatct tttattggtt tagatgcatt ttgggcctct gaaataaatt tagatctact atccatttgt tccagtaccg cttcacactt cgaaatattg aaaaaagcga tggagagaaa tattccttat attttgtgcg aaaagccgat caccgataaa gaagatgagc tcaatgaaat tgccgaactt tctaaaaaat ccaactctaa aatcattgtg aatcatattc ttcgctggga gccaggggtt gattcgatta aagaaaaatt aagccaagag aagattcaga aagtaatagt tttttacgct aagggttttc tccataacgg gattcatgtt gtagatttat ctttatattt gtttggaaat ccgaagaaga taataaaaag aaaagagcat attgatggat ttggatattt aaattttgat ttcgatttag tatatgatag ttttgaaatt tctttttttg ctttagatga agagaaattt agtatttttg aatattctat ttatacggat ttgggtaaat ttgttataga tgagttaact tcaaaaataa aatacttttc aattataaaa catccgaata tcgaagggta tcaatatatt ggttctaaag cagaagagat tgagtgttta caatttcaaa gtatgaaaaa cgttattgat gatgtgattc tcggaattag aactggggat gagtcttatt ttaaatcgga tttaaaggct actttatcta caatgaatat tttattcgat attatgagga ctccatgtca aaactag
Details for LpboA.19557.b.B1.GE41876
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML PLGGKPVLQF LVERLKRSKF IDEIIIATSS NIENDAIESL TKEIGIYCFR GSEGDVLSRV IGAADSRKAD IIVQLTGDCP LMDPELIDEC VSKYLDGGYD YVANELVRTY PIGMDVAVFS LSLLKETYLE KDLTEMDKEH VTTYIVERPI RYKLLNVRAD KEQDRPDLQL TLDTKEDYST LSFIVDSLYT KKSDFNLRDI IQFLDQHKEI SAINSEIKRK KKDIRKLSVG LXXXGKIASD YDSGIDGIEI NSHTKAFLKN EFAEIVVVAD QDISKIQNFK MKWDLEPISF IGLDAFWASE INLDLLSICS STASHFEILK KAMERNIPYI LCEKPITDKE DELNEIAELS KKSNSKIIVN HILRWEPGVD SIKEKLSQEK IQKVIVFYAK GFLHNGIHVV DLSLYLFGNP KKIIKRKEHI DGFGYLNFDF DLVYDSFEIS FFALDEEKFS IFEYSIYTDL GKFVIDELTS KIKYFSIIKH PNIEGYQYIG SKAEEIECLQ FQSMKNVIDD VILGIRTGDE SYFKSDLKAT LSTMNILF
Validated NT Sequence
ancgaataaa atattcattg tagataaagt agcctttaaa tccgatttaa aataagactc atccccagtt ctaattccga gaatcacatc atcaataacg tttttcatac tttgaaattg taaacactca atctcttctg ctttagaacc aatatattga tacccttcga tattcggatg ttttataatt gaaaagtatt ttatttttga agttaactca tctataacaa atttacccaa atccgtataa atagaatatt caaaaatact aaatttctct tcatctaaag caaaaaaaga aatttcaaaa ctatcatata ctaaatcgaa atcaaaattt aaatatccaa atccatcaat atgctctttt ctttttatta tcttcttcgg atttccaaac aaatataaag ataaatctac aacatgaatc ccgttatgga gaaaaccctt agcgtaaaaa actattactt tctgaatctt ctcttggctt aatttttctt taatcgaatc aacccctggc tcccagcgaa gaatatgatt cacaatgatt ttagagttgg attttttaga aagttcggca atttcattga gctcatcttc tttatcggtg atcggctttt cgcacaaaat ataaggaata tttctctcca tcgctttttt caatatttcg aagtgtgaag cggtactgga acaaatggat agtagatcta aatttatttc agaggcccaa aatgcatcta aaccaataaa agatatcggt tctaagtccc atttcatttt aaaattttga attttcgata tatcctgatc tgcaaccacc acgatttccg caaattcatt tttaagaaat gctttcgtat gactattgat ctcgatgccg tcgataccag aatcataatc actggctatt tttccannnn nnatgagacc aacacttaac ttacggatat ctttcttttt tcttttgatt tctgaattga ttgcagaaat ctctttgtgc tgatctaaaa attgaattat atctcttaga ttaaaatcac tttttttcgt atataaagaa tcaacaataa aactcaatgt agaataatct tctttcgtat ccaaagtcag ttgaaggtcc ggacggtcct gttctttatc tgctcgaaca ttcagcaatt tataacgaat cggccgttcc actatataag tagtcacgtg ctctttgtcc atctccgtaa gatccttttc caaatatgtt tcttttaata aggataagga aaaaacagca acatccattc caatcggata agtccgcact aattcgtttg cgacgtaatc gtaaccacca tctaaatatt tactcacgca ttcatcaatc aactcggggt ccatcaaagg acaatctcct gtcaattgaa cgataatatc agctttccta gaatccgctg ctccgatcac acgactcaaa acatcgccct cacttccacg aaaacaataa atcccgatct ccttagtcaa actttcaatt gcatcatttt caatattaga agaggtcgca attatgattt catctataaa cttggaacgt ttgagtcttt cgaccaaaaa ttgaagaaca ggtttcccgc ctaaaggcaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMP LGGKPVLQFL VERLKRSKFI DEIIIATSSN IENDAIESLT KEIGIYCFRG SEGDVLSRVI GAADSRKADI IVQLTGDCPL MDPELIDECV SKYLDGGYDY VANELVRTYP IGMDVAVFSL SLLKETYLEK DLTEMDKEHV TTYIVERPIR YKLLNVRADK EQDRPDLQLT LDTKEDYSTL SFIVDSLYTK KSDFNLRDII QFLDQHKEIS AINSEIKRKK KDIRKLSVGL IGLGKIASDY DSGIDGIEIN SHTKAFLKNE FAEIVVVADQ DISKIQNFKM KWDLEPISFI GLDAFWASEI NLDLLSICSS TASHFEILKK AMERNIPYIL CEKPITDKED ELNEIAELSK KSNSKIIVNH ILRWEPGVDS IKEKLSQEKI QKVIVFYAKG FLHNGIHVVD LSLYLFGNPK KIIKRKEHID GFGYLNFDFD LVYDSFEISF FALDEEKFSI FEYSIYTDLG KFVIDELTSK IKYFSIIKHP NIEGYQYIGS KAEEIECLQF QSMKNVIDDV ILGIRTGDES YFKSDLKATL STMNILFDIM RTPCQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcctt taggcgggaa acctgttctt caatttttgg tcgaaagact caaacgttcc aagtttatag atgaaatcat aattgcgacc tcttctaata ttgaaaatga tgcaattgaa agtttgacta aggagatcgg gatttattgt tttcgtggaa gtgagggcga tgttttgagt cgtgtgatcg gagcagcgga ttctaggaaa gctgatatta tcgttcaatt gacaggagat tgtcctttga tggaccccga gttgattgat gaatgcgtga gtaaatattt agatggtggt tacgattacg tcgcaaacga attagtgcgg acttatccga ttggaatgga tgttgctgtt ttttccttat ccttattaaa agaaacatat ttggaaaagg atcttacgga gatggacaaa gagcacgtga ctacttatat agtggaacgg ccgattcgtt ataaattgct gaatgttcga gcagataaag aacaggaccg tccggacctt caactgactt tggatacgaa agaagattat tctacattga gttttattgt tgattcttta tatacgaaaa aaagtgattt taatctaaga gatataattc aatttttaga tcagcacaaa gagatttctg caatcaattc agaaatcaaa agaaaaaaga aagatatccg taagttaagt gttggtctca ttggccttgg aaaaatagcc agtgattatg attctggtat cgacggcatc gagatcaata gtcatacgaa agcatttctt aaaaatgaat ttgcggaaat cgtggtggtt gcagatcagg atatatcgaa aattcaaaat tttaaaatga aatgggactt agaaccgata tcttttattg gtttagatgc attttgggcc tctgaaataa atttagatct actatccatt tgttccagta ccgcttcaca cttcgaaata ttgaaaaaag cgatggagag aaatattcct tatattttgt gcgaaaagcc gatcaccgat aaagaagatg agctcaatga aattgccgaa ctttctaaaa aatccaactc taaaatcatt gtgaatcata ttcttcgctg ggagccaggg gttgattcga ttaaagaaaa attaagccaa gagaagattc agaaagtaat agttttttac gctaagggtt ttctccataa cgggattcat gttgtagatt tatctttata tttgtttgga aatccgaaga agataataaa aagaaaagag catattgatg gatttggata tttaaatttt gatttcgatt tagtatatga tagttttgaa atttcttttt ttgctttaga tgaagagaaa tttagtattt ttgaatattc tatttatacg gatttgggta aatttgttat agatgagtta acttcaaaaa taaaatactt ttcaattata aaacatccga atatcgaagg gtatcaatat attggttcta aagcagaaga gattgagtgt ttacaatttc aaagtatgaa aaacgttatt gatgatgtga ttctcggaat tagaactggg gatgagtctt attttaaatc ggatttaaag gctactttat ctacaatgaa tattttattc gatattatga ggactccatg tcaaaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at