LpboA.19555.a

Conserved hypothetical lipoprotein

CENTER ID: LpboA.19555.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19555.a.B2.GE41873 soluble domain 40 534
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q04R77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKKSKDSK HTSSTFVEIS YRISAICVLC FFIGCSQLID NGKGPLSLIP GLSVFSDGES PRVIGSWPLN DQTIADPKAA LSISFSKEMN QTFTESALSL KSSQNTIAGS YSWLGSTLHF QPTVSLTDPG IYTLTVSKSQ AEDKQGKNLE QDFILKFNYN ADTIEPTLVD SSPSNGAIGI TSNTHLLLRF SKPMNVNLVL TEITANPSLT FNLPVTIISP DRTTFEFIPI QPLTFGTTYN VTIPNTIKDS IGNPLLQTYR IGFTVGSDFV QPTLTNIRST TNTGNFVLNE FAIIGGFEKT DDFMIDFSES VLPSSLLTGI TFSPSITFTI IENSGGANTS FIIRPNSNLS INQIYQIQVT NSVKDVQGNS LNKTFTYNTQ VNGPRSKYLF VQNIYLSNAH SLANRLLDGV INSLQHDGGI GPTYNYSAGG AKAFYVAFCR DDFRSNTCSE VDLNMVLSSV QFSANFDFGP NSGNPYLLLP QVGAAPNIYK TSIFGLTESP NSYIFTIKGG PDGAKDLYGN YLEQDYSVRF SVSP
NT Sequence
atgagtcgta aaaaatcaaa ggattcgaaa cacacctctt caacgttcgt tgaaatttca tatcggatat ccgcgatttg tgtcctatgt ttttttatcg gatgtagtca gttaatagac aacggaaaag ggcctttgag tttaattccc ggtttatccg tattttcaga cggagaatct ccgagagtga tcggaagctg gccattgaac gaccaaacaa tcgccgatcc aaaagcggct ctttccatct ccttttctaa agagatgaat cagacgttta cggaatccgc tctttctctg aaaagttcac aaaataccat tgcgggaagt tattcctggt tgggttcaac gctgcatttt caacccaccg tctctcttac ggatcctgga atttatactc tcaccgtttc caaatcccag gcggaagata aacaaggcaa aaacttagaa caagatttta tactgaaatt caattataac gcggacacga tcgaaccaac gttagtcgat agttctcctt cgaacggagc catcggaatt acttcaaaca ctcatttact tctcagattt tccaagccaa tgaacgtaaa tctggttctt accgaaatta ccgcaaatcc ttccttaacc ttcaaccttc ccgtgactat catatctccg gatagaacaa cgtttgaatt cataccgatt caacctttga ctttcggtac cacttacaac gtcacaatcc ccaataccat caaagacagt ataggaaatc cgcttttgca gacgtatcga atcggattta ccgtcggttc cgattttgta caacccactc tgacgaacat tagatccact acgaataccg gaaactttgt attgaatgaa ttcgcgatca tcggaggttt cgaaaagacc gacgatttta tgatcgattt ttcggaatcc gtattacctt ccagcttatt gacgggaatc acattcagcc cttcgataac gtttaccatc atcgaaaatt cgggaggggc taataccagt tttattatac gtccaaattc gaatttatcg atcaatcaaa tataccagat acaggtgacc aattccgtca aagacgttca aggaaatagt ctgaataaaa cgttcactta taatactcaa gtgaacggtc ctagaagtaa gtatcttttc gtacagaata tctatctgag caacgcgcat tctctcgcaa atcgattgtt agacggtgta atcaattcgc ttcagcacga cggtggtata ggtcctacgt ataattattc tgcgggaggc gcgaaagcgt tttacgtagc cttttgcagg gacgattttc ggtcgaacac ttgttccgaa gtcgatttga atatggtatt gtcttctgta caattttcgg cgaacttcga cttcggaccc aattccggaa atccttatct gcttctacct caggtaggag cggcgcctaa catctataaa acttccatct tcggactcac ggaatctccg aactcttaca tttttacgat aaaaggcgga ccggacggag ctaaagattt gtacggaaat tatctggaac aggattactc cgtcagattt tccgtttccc cttaa
Details for LpboA.19555.a.B2.GE41873
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHHH MDNGKGPLSL IPGLSVFSDG ESPRVIGSWP LNDQTIADPK AALSISFSKE MNQTFTESAL SLKSSQNTIA GSYSWLGSTL HFQPTVSLTD PGIYTLTVSK SQAEDKQGKN LEQDFILKFN YNADTIEPTL VDSSPSNGAI GITSNTHLLL RFSKPMNVNL VLTEITANPS LTFNLPVTII SPDRTTFEFI PIQPLTFGTT YNVTIPNTIK DSIGNPLLQT YRIGFTVGSD FVQPTLTNIR STTNTGNFVL NEFAIIGGFE KTDDFMIDFS ESVLPSSLLT GITFSPSITF TIIENSGGAN TSFIIRPNSN LSINQIYQIQ VTNSVKDVQG NSLNKTFTYN TQVNGPRSKY LFVQNIYLSN AHSLANRLLD GVINSLQHDG GIGPTYNYSA GGAKAFYVAF CRDDFRSNTC SEVDLNMVLS SVQFSANFDF GPNSGNPYLL LPQVGAAPNI YKTSIFGLTE SPNSYIFTIK GGPDGAKDLY GN
Validated NT Sequence
taatttccgt acaaatcttt agctccgtcc ggtccgcctt ttatcgtaaa aatgtaagag ttcggagatt ccgtgagtcc gaagatggaa gttttataga tgttaggcgc cgctcctacc tgaggtagaa gcagataagg atttccggaa ttgggtccga agtcgaagtt cgccgaaaat tgtacagaag acaataccat attcaaatcg acttcggaac aagtgttcga ccgaaaatcg tccctgcaaa aggctacgta aaacgctttc gcgcctcccg cagaataatt atacgtagga cctataccac cgtcgtgctg aagcgaattg attacaccgt ctaacaatcg atttgcgaga gaatgcgcgt tgctcagata gatattctgt acgaaaagat acttacttct aggaccgttc acttgagtat tataagtgaa cgttttattc agactatttc cttgaacgtc tttgacggaa ttggtcacct gtatctggta tatttgattg atcgataaat tcgaatttgg acgtataata aaactggtat tagcccctcc cgaattttcg atgatggtaa acgttatcga agggctgaat gtgattcccg tcaataagct ggaaggtaat acggattccg aaaaatcgat cataaaatcg tcggtctttt cgaaacctcc gatgatcgcg aattcattca atacaaagtt tccggtattc gtagtggatc taatgttcgt cagagtgggt tgtacaaaat cggaaccgac ggtaaatccg attcgatacg tctgcaaaag cggatttcct atactgtctt tgatggtatt ggggattgtg acgttgtaag tggtaccgaa agtcaaaggt tgaatcggta tgaattcaaa cgttgttcta tccggagata tgatagtcac gggaaggttg aaggttaagg aaggatttgc ggtaatttcg gtaagaacca gatttacgtt cattggcttg gaaaatctga gaagtaaatg agtgtttgaa gtaattccga tggctccgtt cgaaggagaa ctatcgacta acgttggttc gatcgtgtcc gcgttataat tgaatttcag tataaaatct tgttctaagt ttttgccttg tttatcttcc gcctgggatt tggaaacggt gagagtataa attccaggat ccgtaagaga gacggtgggt tgaaaatgca gcgttgaacc caaccaggaa taacttcccg caatggtatt ttgtgaactt ttcagagaaa gagcggattc cgtaaacgtc tgattcatct ctttagaaaa ggagatggaa agagccgctt ttggatcggc gattgtttgg tcgttcaatg gccagcttcc gatcactctc ggagattctc cgtctgaaaa tacggataaa ccgggaatta aactcaaagg cccttttccg ttgtccatat ggtggtggtg gtggtggtgg tgagccatgg tata
Expected Protein Sequence
MAHHHHHHDN GKGPLSLIPG LSVFSDGESP RVIGSWPLND QTIADPKAAL SISFSKEMNQ TFTESALSLK SSQNTIAGSY SWLGSTLHFQ PTVSLTDPGI YTLTVSKSQA EDKQGKNLEQ DFILKFNYNA DTIEPTLVDS SPSNGAIGIT SNTHLLLRFS KPMNVNLVLT EITANPSLTF NLPVTIISPD RTTFEFIPIQ PLTFGTTYNV TIPNTIKDSI GNPLLQTYRI GFTVGSDFVQ PTLTNIRSTT NTGNFVLNEF AIIGGFEKTD DFMIDFSESV LPSSLLTGIT FSPSITFTII ENSGGANTSF IIRPNSNLSI NQIYQIQVTN SVKDVQGNSL NKTFTYNTQV NGPRSKYLFV QNIYLSNAHS LANRLLDGVI NSLQHDGGIG PTYNYSAGGA KAFYVAFCRD DFRSNTCSEV DLNMVLSSVQ FSANFDFGPN SGNPYLLLPQ VGAAPNIYKT SIFGLTESPN SYIFTIKGGP DGAKDLYGNY LEQDYSVRFS VSP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacaacg gaaaagggcc tttgagttta attcccggtt tatccgtatt ttcagacgga gaatctccga gagtgatcgg aagctggcca ttgaacgacc aaacaatcgc cgatccaaaa gcggctcttt ccatctcctt ttctaaagag atgaatcaga cgtttacgga atccgctctt tctctgaaaa gttcacaaaa taccattgcg ggaagttatt cctggttggg ttcaacgctg cattttcaac ccaccgtctc tcttacggat cctggaattt atactctcac cgtttccaaa tcccaggcgg aagataaaca aggcaaaaac ttagaacaag attttatact gaaattcaat tataacgcgg acacgatcga accaacgtta gtcgatagtt ctccttcgaa cggagccatc ggaattactt caaacactca tttacttctc agattttcca agccaatgaa cgtaaatctg gttcttaccg aaattaccgc aaatccttcc ttaaccttca accttcccgt gactatcata tctccggata gaacaacgtt tgaattcata ccgattcaac ctttgacttt cggtaccact tacaacgtca caatccccaa taccatcaaa gacagtatag gaaatccgct tttgcagacg tatcgaatcg gatttaccgt cggttccgat tttgtacaac ccactctgac gaacattaga tccactacga ataccggaaa ctttgtattg aatgaattcg cgatcatcgg aggtttcgaa aagaccgacg attttatgat cgatttttcg gaatccgtat taccttccag cttattgacg ggaatcacat tcagcccttc gataacgttt accatcatcg aaaattcggg aggggctaat accagtttta ttatacgtcc aaattcgaat ttatcgatca atcaaatata ccagatacag gtgaccaatt ccgtcaaaga cgttcaagga aatagtctga ataaaacgtt cacttataat actcaagtga acggtcctag aagtaagtat cttttcgtac agaatatcta tctgagcaac gcgcattctc tcgcaaatcg attgttagac ggtgtaatca attcgcttca gcacgacggt ggtataggtc ctacgtataa ttattctgcg ggaggcgcga aagcgtttta cgtagccttt tgcagggacg attttcggtc gaacacttgt tccgaagtcg atttgaatat ggtattgtct tctgtacaat tttcggcgaa cttcgacttc ggacccaatt ccggaaatcc ttatctgctt ctacctcagg taggagcggc gcctaacatc tataaaactt ccatcttcgg actcacggaa tctccgaact cttacatttt tacgataaaa ggcggaccgg acggagctaa agatttgtac ggaaattatc tggaacagga ttactccgtc agattttccg tttccccttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat