LpboA.19554.b

Uncharacterized protein

CENTER ID: LpboA.19554.b
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19554.b.B1.GE41872 full length 1 216
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.3939
UniProt: Q04NR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERLKREIAR YPRGYWIKAG AESIVLCRNL TILGNLQAGA VNPMLPFIFL SVWDGIASNA NRQVFNGSTY VNYFGYYYNY DPRRVISHEL THSVDRRNSW HTLFDPEWER LNHPGFQYGN HNYNPQDSIL RISNPIPGFV SYYATLNHLE DRAESGMVIM GPQAINNQLV QTCQNDAIVA AKVRKTVSEW KQFWPFAGAE NTEWKVRMSQ AEQDCS
NT Sequence
gtggaaagat taaaaagaga gattgccagg tatccgaggg gatattggat caaagcgggt gcggaaagca tagttctctg tagaaactta acaattttgg gtaaccttca ggctggagcg gtcaatccta tgttaccatt tattttttta agcgtgtggg atggaattgc atccaatgcc aaccgacaag tattcaatgg tagtacctac gtaaattact ttggttatta ttacaattat gatcctcggc gggtgatcag ccacgaactg acacattcag tggatcgtcg taactcatgg cataccttgt ttgatccgga atgggagagg ttgaatcatc cgggatttca atacggaaat cacaactaca atccgcaaga tagtattctt cgtatttcga atcctattcc gggttttgtg tcctattacg cgactctaaa tcatttagag gatcgagcgg aaagcggaat ggtaatcatg gggccacaag ccattaacaa tcaattggtt caaacctgtc aaaacgatgc gatcgttgct gcaaaggttc gtaagacggt ctcggaatgg aaacaatttt ggccttttgc aggcgcggag aatacggagt ggaaggtcag aatgtctcaa gctgaacagg attgcagctg a
Details for LpboA.19554.b.B1.GE41872
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV ERLKREIARY PRGYWIKAGA ESIVLCRNLT ILGNLQAGAV NPMLPFIFLS VWDGIASNAN RQVFNGSTYV NYFGYYYNYD PRRVISHELT HSVDRRNSWH TLFDPEWERL NHPGFQYGNH NYNPQDSILR ISNPIPGFVS YYATLNHLED RAESGMVIMG PQAINNQLVQ TCQNDAIVAA KVRKTVSEWK QFWPFAGAEN TEWKVRMSQA EQDCS
Validated NT Sequence
atcttactca cttagctgca atcctgttca gcttgagaca ttctgacctt ccactccgta ttctccgcgc ctgcaaaagg ccaaaattgt ttccattccg agaccgtctt acgaaccttt gcagcaacga tcgcatcgtt ttgacaggtt tgaaccaatt gattgttaat ggcttgtggc cccatgatta ccattccgct ttccgctcga tcctctaaat gatttagagt cgcgtaatag gacacaaaac ccggaatagg attcgaaata cgaagaatac tatcttgcgg attgtagttg tgatttccgt attgaaatcc cggatgattc aacctctccc attccggatc aaacaaggta tgccatgagt tacgacgatc cactgaatgt gtcagttcgt ggctgatcac ccgccgagga tcataattgt aataataacc aaagtaattt acgtaggtac taccattgaa tacttgtcgg ttggcattgg atgcaattcc atcccacacg cttaaaaaaa taaatggtaa cataggattg accgctccag cctgaaggtt acccaaaatt gttaagtttc tacagagaac tatgctttcc gcacccgctt tgatccaata tcccctcgga tacctggcaa tctctctttt taatctttcc accatatggt ggtggtggtg gtgagccatg gta
Expected Protein Sequence
MAHHHHHHME RLKREIARYP RGYWIKAGAE SIVLCRNLTI LGNLQAGAVN PMLPFIFLSV WDGIASNANR QVFNGSTYVN YFGYYYNYDP RRVISHELTH SVDRRNSWHT LFDPEWERLN HPGFQYGNHN YNPQDSILRI SNPIPGFVSY YATLNHLEDR AESGMVIMGP QAINNQLVQT CQNDAIVAAK VRKTVSEWKQ FWPFAGAENT EWKVRMSQAE QDCS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggaaa gattaaaaag agagattgcc aggtatccga ggggatattg gatcaaagcg ggtgcggaaa gcatagttct ctgtagaaac ttaacaattt tgggtaacct tcaggctgga gcggtcaatc ctatgttacc atttattttt ttaagcgtgt gggatggaat tgcatccaat gccaaccgac aagtattcaa tggtagtacc tacgtaaatt actttggtta ttattacaat tatgatcctc ggcgggtgat cagccacgaa ctgacacatt cagtggatcg tcgtaactca tggcatacct tgtttgatcc ggaatgggag aggttgaatc atccgggatt tcaatacgga aatcacaact acaatccgca agatagtatt cttcgtattt cgaatcctat tccgggtttt gtgtcctatt acgcgactct aaatcattta gaggatcgag cggaaagcgg aatggtaatc atggggccac aagccattaa caatcaattg gttcaaacct gtcaaaacga tgcgatcgtt gctgcaaagg ttcgtaagac ggtctcggaa tggaaacaat tttggccttt tgcaggcgcg gagaatacgg agtggaaggt cagaatgtct caagctgaac aggattgcag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat