LpboA.19554.a

Uncharacterized protein

CENTER ID: LpboA.19554.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LpboA.19554.a.B1.GE41871 full length 1 246
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LpboA.19554.a.B1.PS38509 full length 1 246
Molecular models
METHOD RESULTS
comparative modelling Robetta_2682
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|355277.4.peg.2607
UniProt: Q04RC7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLTKLLRS IVSMKQESMK NSFFNNSNTS LGSARYPRGY WIKAGVESVT LCRNLKVLGG LAAGTTGFTG SIFLNVKDRI ASYDSIIFDY STGTWINYLD TDDGYASDPR GTIHHELTHA VDHHVPGFIT FFDPEWGKLN YPGFQYGNSY DPKNPPVEIV HNMNPVPGFV SYYATERHIE DRAEIGRAIM ARRSTYNRLV RICQTDPIVA AKVRKTVSEW NQFWPFPGAE NTEWKTRMIQ AEQDCR
NT Sequence
ttgtctagat taacaaaact gcttcgatcg atcgtttcca tgaaacagga atcaatgaag aattcatttt tcaacaattc gaatacatcc ctaggaagcg ccagatatcc aaggggttat tggatcaaag caggcgtgga aagcgttacg ctttgtagaa atttaaaggt tttgggcggg ctcgccgctg gaacgactgg ctttactggt tccatattct tgaatgtaaa agatcgaatt gcatcctatg actctatcat attcgattat agcactggca cctggataaa ttaccttgat actgatgatg gttatgcttc ggatcctcgg ggtacaatcc atcacgaact aacacacgca gtggatcatc acgtcccagg ttttatcacg tttttcgatc cagaatgggg caagttaaat tatcccggat ttcaatacgg aaatagttat gatcccaaaa atcctcctgt cgagattgtt cataatatga accctgttcc cggtttcgta tcctattacg cgacagagag acatatagaa gaccgagctg aaattggaag ggcaattatg gcacgtcgga gtacgtacaa ccgattggtt cgaatctgcc aaaccgatcc aatcgttgcg gcaaaagtac gtaaaacggt ttccgaatgg aaccagttct ggccctttcc aggtgcggaa aatacggagt ggaaaacaag aatgatccaa gcagaacagg attgccgatg a
Details for LpboA.19554.a.B1.GE41871
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML SRLTKLLRSI VSMKQESMKN SFFNNSNTSL GSARYPRGYW IKAGVESVTL CRNLKVLGGL AAGTTGFTGS IFLNVKDRIA SYDSIIFDYS TGTWINYLDT DDGYASDPRG TIHHELTHAV DHHVPGFITF FDPEWGKLNY PGFQYGNSYD PKNPPVEIVH NMNPVPGFVS YYATERHIED RAEIGRAIMA RRSTYNRLVR ICQTDPIVAA KVRKTVSEWN QFWPFPGAEN TEWKTRMIQA EQDCR
Validated NT Sequence
atcttactca cttatcggca atcctgttct gcttggatca ttcttgtttt ccactccgta ttttccgcac ctggaaaggg ccagaactgg ttccattcgg aaaccgtttt acgtactttt gccgcaacga ttggatcggt ttggcagatt cgaaccaatc ggttgtacgt actccgacgt gccataattg cccttccaat ttcagctcgg tcttctatat gtctctctgt cgcgtaatag gatacgaaac cgggaacagg gttcatatta tgaacaatct cgacaggagg atttttggga tcataactat ttccgtattg aaatccggga taatttaact tgccccattc tggatcgaaa aacgtgataa aacctgggac gtgatgatcc actgcgtgtg ttagttcgtg atggattgta ccccgaggat ccgaagcata accatcatca gtatcaaggt aatttatcca ggtgccagtg ctataatcga atatgataga gtcataggat gcaattcgat cttttacatt caagaatatg gaaccagtaa agccagtcgt tccagcggcg agcccgccca aaacctttaa atttctacaa agcgtaacgc tttccacgcc tgctttgatc caataacccc ttggatatct ggcgcttcct agggatgtat tcgaattgtt gaaaaatgaa ttcttcattg attcctgttt catggaaacg atcgatcgaa gcagttttgt taatctagac aacatatggt ggtggtggtg gtgagccatg gta
Expected Protein Sequence
MAHHHHHHMS RLTKLLRSIV SMKQESMKNS FFNNSNTSLG SARYPRGYWI KAGVESVTLC RNLKVLGGLA AGTTGFTGSI FLNVKDRIAS YDSIIFDYST GTWINYLDTD DGYASDPRGT IHHELTHAVD HHVPGFITFF DPEWGKLNYP GFQYGNSYDP KNPPVEIVHN MNPVPGFVSY YATERHIEDR AEIGRAIMAR RSTYNRLVRI CQTDPIVAAK VRKTVSEWNQ FWPFPGAENT EWKTRMIQAE QDCR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtcta gattaacaaa actgcttcga tcgatcgttt ccatgaaaca ggaatcaatg aagaattcat ttttcaacaa ttcgaataca tccctaggaa gcgccagata tccaaggggt tattggatca aagcaggcgt ggaaagcgtt acgctttgta gaaatttaaa ggttttgggc gggctcgccg ctggaacgac tggctttact ggttccatat tcttgaatgt aaaagatcga attgcatcct atgactctat catattcgat tatagcactg gcacctggat aaattacctt gatactgatg atggttatgc ttcggatcct cggggtacaa tccatcacga actaacacac gcagtggatc atcacgtccc aggttttatc acgtttttcg atccagaatg gggcaagtta aattatcccg gatttcaata cggaaatagt tatgatccca aaaatcctcc tgtcgagatt gttcataata tgaaccctgt tcccggtttc gtatcctatt acgcgacaga gagacatata gaagaccgag ctgaaattgg aagggcaatt atggcacgtc ggagtacgta caaccgattg gttcgaatct gccaaaccga tccaatcgtt gcggcaaaag tacgtaaaac ggtttccgaa tggaaccagt tctggccctt tccaggtgcg gaaaatacgg agtggaaaac aagaatgatc caagcagaac aggattgccg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for LpboA.19554.a.B1.PS38509
PURIFICATION DATe: 9/25/2018
CONCENTRATION: 20.18mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML SRLTKLLRSI VSMKQESMKN SFFNNSNTSL GSARYPRGYW IKAGVESVTL CRNLKVLGGL AAGTTGFTGS IFLNVKDRIA SYDSIIFDYS TGTWINYLDT DDGYASDPRG TIHHELTHAV DHHVPGFITF FDPEWGKLNY PGFQYGNSYD PKNPPVEIVH NMNPVPGFVS YYATERHIED RAEIGRAIMA RRSTYNRLVR ICQTDPIVAA KVRKTVSEWN QFWPFPGAEN TEWKTRMIQA EQDCR
Validated NT Sequence
atcttactca cttatcggca atcctgttct gcttggatca ttcttgtttt ccactccgta ttttccgcac ctggaaaggg ccagaactgg ttccattcgg aaaccgtttt acgtactttt gccgcaacga ttggatcggt ttggcagatt cgaaccaatc ggttgtacgt actccgacgt gccataattg cccttccaat ttcagctcgg tcttctatat gtctctctgt cgcgtaatag gatacgaaac cgggaacagg gttcatatta tgaacaatct cgacaggagg atttttggga tcataactat ttccgtattg aaatccggga taatttaact tgccccattc tggatcgaaa aacgtgataa aacctgggac gtgatgatcc actgcgtgtg ttagttcgtg atggattgta ccccgaggat ccgaagcata accatcatca gtatcaaggt aatttatcca ggtgccagtg ctataatcga atatgataga gtcataggat gcaattcgat cttttacatt caagaatatg gaaccagtaa agccagtcgt tccagcggcg agcccgccca aaacctttaa atttctacaa agcgtaacgc tttccacgcc tgctttgatc caataacccc ttggatatct ggcgcttcct agggatgtat tcgaattgtt gaaaaatgaa ttcttcattg attcctgttt catggaaacg atcgatcgaa gcagttttgt taatctagac aacatatggt ggtggtggtg gtgagccatg gta
Expressed Protein Sequence
MAHHHHHHMS RLTKLLRSIV SMKQESMKNS FFNNSNTSLG SARYPRGYWI KAGVESVTLC RNLKVLGGLA AGTTGFTGSI FLNVKDRIAS YDSIIFDYST GTWINYLDTD DGYASDPRGT IHHELTHAVD HHVPGFITFF DPEWGKLNYP GFQYGNSYDP KNPPVEIVHN MNPVPGFVSY YATERHIEDR AEIGRAIMAR RSTYNRLVRI CQTDPIVAAK VRKTVSEWNQ FWPFPGAENT EWKTRMIQAE QDCR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGTCTA GATTAACAAA ACTGCTTCGA TCGATCGTTT CCATGAAACA GGAATCAATG AAGAATTCAT TTTTCAACAA TTCGAATACA TCCCTAGGAA GCGCCAGATA TCCAAGGGGT TATTGGATCA AAGCAGGCGT GGAAAGCGTT ACGCTTTGTA GAAATTTAAA GGTTTTGGGC GGGCTCGCCG CTGGAACGAC TGGCTTTACT GGTTCCATAT TCTTGAATGT AAAAGATCGA ATTGCATCCT ATGACTCTAT CATATTCGAT TATAGCACTG GCACCTGGAT AAATTACCTT GATACTGATG ATGGTTATGC TTCGGATCCT CGGGGTACAA TCCATCACGA ACTAACACAC GCAGTGGATC ATCACGTCCC AGGTTTTATC ACGTTTTTCG ATCCAGAATG GGGCAAGTTA AATTATCCCG GATTTCAATA CGGAAATAGT TATGATCCCA AAAATCCTCC TGTCGAGATT GTTCATAATA TGAACCCTGT TCCCGGTTTC GTATCCTATT ACGCGACAGA GAGACATATA GAAGACCGAG CTGAAATTGG AAGGGCAATT ATGGCACGTC GGAGTACGTA CAACCGATTG GTTCGAATCT GCCAAACCGA TCCAATCGTT GCGGCAAAAG TACGTAAAAC GGTTTCCGAA TGGAACCAGT TCTGGCCCTT TCCAGGTGCG GAAAATACGG AGTGGAAAAC AAGAATGATC CAAGCAGAAC AGGATTGCCG ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT