LpboA.19552.b

Uncharacterized protein

CENTER ID: LpboA.19552.b
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19552.b.B1.GE41870 full length 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.166
UniProt: Q04W35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIEVKKIKS SKISDLTDFL NRYPWSQEWT QFEKPIDSLW EFELDIKVET LWPWLLDTSS FNKRIGIPEM KFVEKEGKLF GRSKNAGILM EWEEIPWEWE YCKELNNARI YKKGLARYVR SRYILEKLSE NKTKLIVYFG WIPKGFLGKL ILLFEMKRLY QNYRKGLSGV LEDINARKKD ESPIFNFNKN LKEAAPISEN LKLKQIKTNL IREGIDPDLI DRVIHYILFE DENELYRIRI KKLTFEWKLP LETLLALFLH GCKQGLFTLS WDVICPHCRG VRSELVNLGD VPLKDSCDVC GIDFESTKLN TIEVTFHIHP SIRKIQKRYF CAAEPATKAH IWFQRTVQPG KEYVTNLLLN EGIFRLRVAG KKRYNLPHRT ISIGPQINWK KS
NT Sequence
ttgagcatag aggtaaaaaa aatcaaatcc tcaaaaatat cggatcttac tgatttttta aatagatatc cctggtcgca agaatggact caattcgaaa aaccgatcga ttcactctgg gaattcgaac tcgacataaa agttgaaact ctttggcctt ggctcctaga cacttcttcg tttaacaaaa ggatcggaat tccagaaatg aagttcgttg aaaaagaggg aaaacttttc ggccgttcca aaaatgcagg aattctaatg gaatgggagg aaattccttg ggaatgggaa tattgcaaag agttgaacaa cgcaagaatt tataaaaaag gtttagctcg ttatgttaga agtaggtata tactggaaaa actttcagaa aataaaacga aactcatcgt atatttcgga tggattccca aaggttttct cggtaaactc attttgctct ttgaaatgaa acggctctat caaaattacc gaaaaggtct ttctggagtt ttagaagaca ttaatgcgcg aaagaaagac gaatcaccca ttttcaactt caacaaaaac ctaaaagaag ccgcaccgat atcggaaaat ctaaaattga aacaaatcaa aacaaatctg attcgagaag gaatagatcc cgacctaatc gaccgagtga tccattatat tcttttcgaa gacgagaacg aactttatcg aattcgaatt aaaaaattga ctttcgaatg gaaacttcca ttggaaactc ttctcgcttt atttttacat ggatgcaagc agggtctatt tactttatct tgggacgtta tctgccctca ttgcaggggg gtgcgttcgg aactcgttaa cttaggagac gttcctctta aggactcctg cgacgtatgc ggtatagatt tcgaatccac aaaactcaac acgatcgaag ttacatttca cattcatcca tccatccgaa aaatacaaaa aaggtatttc tgtgcggccg aacctgcgac aaaagcgcac atctggtttc aaagaaccgt ccaaccggga aaagaatatg ttacaaattt attattgaac gaaggaattt ttcgcctcag agttgcggga aaaaaaagat acaatcttcc tcatcggacc atttccattg ggccgcagat caattggaag aaaagttaa
Details for LpboA.19552.b.B1.GE41870
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHML SIEVKKIKSS KISDLTDFLN RYPWSQEWTQ FEKPIDSLWE FELDIKVETL WPWLLDTSSF NKRIGIPEMK FVEKEGKLFG RSKNAGILME WEEIPWEWEY CKELNNARIY KKGLARYVRS RYILEKLSEN KTKLIVYFGW IPKGFLGKLI LLFEMKRLYQ NYRKGLSGVL EDINARKKDE SPIFNFNKNL KEAAPISENL KLKQIKTNLI REGIDPDLID RVIHYILFED ENELYRIRIK KLTFEWKLPL ETLLALFLHG CKQGLFTLSW DVICPHCRGV RSELVNLGDV PLKDSCDVCG IDFESTKLNT IEVTFHIHPS IRKIQKRYFC AAEPATKAHI WFQRTVQPGK EYVTNLLLNE GIFRLRVAGK KRYNLP
Validated NT Sequence
atgaggaaga ttgtatcttt tttttcccgc aactctgagg cgaaaaattc cttcgttcaa taataaattt gtaacatatt cttttcccgg ttggacggtt ctttgaaacc agatgtgcgc ttttgtcgca ggttcggccg cacagaaata ccttttttgt atttttcgga tggatggatg aatgtgaaat gtaacttcga tcgtgttgag ttttgtggat tcgaaatcta taccgcatac gtcgcaggag tccttaagag gaacgtctcc taagttaacg agttccgaac gcacccccct gcaatgaggg cagataacgt cccaagataa agtaaataga ccctgcttgc atccatgtaa aaataaagcg agaagagttt ccaatggaag tttccattcg aaagtcaatt ttttaattcg aattcgataa agttcgttct cgtcttcgaa aagaatataa tggatcactc ggtcgattag gtcgggatct attccttctc gaatcagatt tgttttgatt tgtttcaatt ttagattttc cgatatcggt gcggcttctt ttaggttttt gttgaagttg aaaatgggtg attcgtcttt ctttcgcgca ttaatgtctt ctaaaactcc agaaagacct tttcggtaat tttgatagag ccgtttcatt tcaaagagca aaatgagttt accgagaaaa cctttgggaa tccatccgaa atatacgatg agtttcgttt tattttctga aagtttttcc agtatatacc tacttctaac ataacgagct aaaccttttt tataaattct tgcgttgttc aactctttgc aatattccca ttcccaagga atttcctccc attccattag aattcctgca tttttggaac ggccgaaaag ttttccctct ttttcaacga acttcatttc tggaattccg atccttttgt taaacgaaga agtgtctagg agccaaggcc aaagagtttc aacttttatg tcgagttcga attcccagag tgaatcgatc ggtttttcga attgagtcca ttcttgcgac cagggatatc tatttaaaaa atcagtaaga tccgatattt ttgaggattt gatttttttt acctctatgc tcaacatatg gtggtggtgg tggtgagcca tggtata
Expected Protein Sequence
MAHHHHHHMS IEVKKIKSSK ISDLTDFLNR YPWSQEWTQF EKPIDSLWEF ELDIKVETLW PWLLDTSSFN KRIGIPEMKF VEKEGKLFGR SKNAGILMEW EEIPWEWEYC KELNNARIYK KGLARYVRSR YILEKLSENK TKLIVYFGWI PKGFLGKLIL LFEMKRLYQN YRKGLSGVLE DINARKKDES PIFNFNKNLK EAAPISENLK LKQIKTNLIR EGIDPDLIDR VIHYILFEDE NELYRIRIKK LTFEWKLPLE TLLALFLHGC KQGLFTLSWD VICPHCRGVR SELVNLGDVP LKDSCDVCGI DFESTKLNTI EVTFHIHPSI RKIQKRYFCA AEPATKAHIW FQRTVQPGKE YVTNLLLNEG IFRLRVAGKK RYNLPHRTIS IGPQINWKKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagca tagaggtaaa aaaaatcaaa tcctcaaaaa tatcggatct tactgatttt ttaaatagat atccctggtc gcaagaatgg actcaattcg aaaaaccgat cgattcactc tgggaattcg aactcgacat aaaagttgaa actctttggc cttggctcct agacacttct tcgtttaaca aaaggatcgg aattccagaa atgaagttcg ttgaaaaaga gggaaaactt ttcggccgtt ccaaaaatgc aggaattcta atggaatggg aggaaattcc ttgggaatgg gaatattgca aagagttgaa caacgcaaga atttataaaa aaggtttagc tcgttatgtt agaagtaggt atatactgga aaaactttca gaaaataaaa cgaaactcat cgtatatttc ggatggattc ccaaaggttt tctcggtaaa ctcattttgc tctttgaaat gaaacggctc tatcaaaatt accgaaaagg tctttctgga gttttagaag acattaatgc gcgaaagaaa gacgaatcac ccattttcaa cttcaacaaa aacctaaaag aagccgcacc gatatcggaa aatctaaaat tgaaacaaat caaaacaaat ctgattcgag aaggaataga tcccgaccta atcgaccgag tgatccatta tattcttttc gaagacgaga acgaacttta tcgaattcga attaaaaaat tgactttcga atggaaactt ccattggaaa ctcttctcgc tttattttta catggatgca agcagggtct atttacttta tcttgggacg ttatctgccc tcattgcagg ggggtgcgtt cggaactcgt taacttagga gacgttcctc ttaaggactc ctgcgacgta tgcggtatag atttcgaatc cacaaaactc aacacgatcg aagttacatt tcacattcat ccatccatcc gaaaaataca aaaaaggtat ttctgtgcgg ccgaacctgc gacaaaagcg cacatctggt ttcaaagaac cgtccaaccg ggaaaagaat atgttacaaa tttattattg aacgaaggaa tttttcgcct cagagttgcg ggaaaaaaaa gatacaatct tcctcatcgg accatttcca ttgggccgca gatcaattgg aagaaaagtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat