LpboA.19552.a

Adenylate cyclase domain protein

CENTER ID: LpboA.19552.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19552.a.B1.GE41869 full length 1 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19552.a.B1.PS38489 full length 1 490

Molecular models

METHOD RESULTS
comparative modelling Robetta_2686

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.2766
UniProt: Q04R04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWNETLFEQK KKSLEGFGVL SKKSIARFMD NIRVLDEWQL HRINPIRFAK QNDFEIGETL DLFLHSAKIG FLDFAYNMIC PACGGVAASH TSLDQIEEKS FHCYICNIDV PTTLDDQVEV SFSANPSLKK QSLNPLVDVE TYLRYHISAN FCKSKELLNF IFSNIQDLIV MEPGETKRIH LDATNVPVYQ FSSVENNSAV FLYFDSKEVT KDRIIDLSLL PTGFTPVELH LSPGEYEVKV SNRTIATSGF LIIKPNLKRI LEIIGEHPTV IQPFLTAKML LNNQTFRELF RVQRLSSQLN LNVKSLTILF TDLRGSTEIY DKAGDILAYR LVQEHFRLLA ETVKKFNGAI VKTMGDAVMA TFSSPLEGLF ASLEMMFRID RMNEEFKEHG HEIGLKVGLN EGSALAVIND ERLDYFGQSV NIAARVQALA SAGEIWVTEP ILSSPGIQEE LNLKGYGSER YEAFLKGVGQ KAAVHKLFKN EEQRAFVGSV
NT Sequence
atgtggaatg aaactctttt cgaacaaaag aaaaaatctt tggaaggatt cggagtttta tcgaaaaaat ccattgctcg atttatggac aacataaggg ttctggacga atggcagctc catcggatta atccgattcg ttttgcaaag cagaatgatt ttgaaatagg agagacgctc gatcttttcc ttcattccgc aaagatcggt tttcttgatt ttgcgtacaa catgatttgc cccgcttgtg gaggtgttgc cgcttctcac acttctttgg atcaaatcga ggaaaaaagt tttcattgtt atatctgtaa catagacgtg ccgactactt tggatgatca ggtggaagtc agcttttcgg cgaacccgtc ccttaagaaa caatccttga atcctttggt ggatgtggaa acgtatctca gatatcatat ttccgccaac ttttgcaagt ccaaagagct tctgaatttc attttttcga atattcaaga tttaatcgtt atggaaccgg gggagacaaa acgaatccat ttggatgcga cgaacgttcc cgtgtatcaa ttcagttctg tggaaaataa ttcggccgtt tttttgtatt ttgattctaa ggaagtcacg aaggatcgga tcatcgacct cagtcttctt cctaccggat ttactcccgt tgaattacat ctttcaccgg gggagtacga ggtgaaagtt tccaaccgga cgatcgcaac ctccggcttt ttaatcatta aaccaaactt gaaaagaatc ctggaaatta tcggggaaca tccgacggtg atccaaccgt ttctgaccgc gaagatgctt ttgaacaatc aaaccttccg ggaattattc cgcgttcaac gattgagcag tcaattgaat ttaaacgtaa aaagtcttac gatccttttt acggatttga gaggctcgac cgaaatatac gataaagcgg gggatatcct tgcatatcga ttggttcagg aacatttccg tttgctcgcc gaaaccgtaa agaagtttaa cggcgccatc gtaaaaacga tgggagacgc ggttatggcg accttttcta gtccgctgga aggactattt gcttcattag aaatgatgtt tcgtatcgat cggatgaacg aggaatttaa ggaacacgga catgagatcg gtttgaaggt cggtttaaat gaaggttctg ctcttgccgt tatcaacgac gaaagacttg attacttcgg tcagagcgta aatattgcgg caagagtgca agcgcttgca tctgcgggag aaatctgggt gactgagccg attctttcca gtcctggaat tcaggaagaa ttaaatctga aaggatatgg atccgaaaga tatgaggctt ttttgaaagg agtcggtcaa aaagccgccg ttcacaaact gtttaagaac gaggaacaaa gggcgtttgt agggtcggtt taa
Details for LpboA.19552.a.B1.GE41869
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 56kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMW NETLFEQKKK SLEGFGVLSK KSIARFMDNI RVLDEWQLHR INPIRFAKQN DFEIGETLDL FLHSAKIGFL DFAYNMICPA CGGVAASHTS LDQIEEKSFH CYICNIDVPT TLDDQVEVSF SANPSLKKQS LNPLVDVETY LRYHISANFC KSKELLNFIF SNIQDLIVME PGETKRIHLD ATNVPVYQFS SVENNSAVFL YFDSKEVTKD RIIDLSLLPT GFTPVELHLS PGEYEVKVSN RTIATSGFLI IKPNLKRILE IIGEHPTVIQ PFLTAKMLLN NQTFRELFRV QRLSSQLNLN VKSLTILFTD LRGSTEIYDK AGDILAYRLV QEHFRLLAET VKKFNGAIVK TMGDAVMATF SSPLEGLFAS LEMMFRIDRM NEEFKEHGHE IGLKVGLNEG SALAVINDER LDYFGQSVNI AARVQALASA GEIWVTEPIL SSPGIQEELN LKGYGSERYE AFLKGVGQKA AVHKLFKNE
Validated NT Sequence
tcctcgttct taaacagttt gtgaacggcg gctttttgac cgactccttt caaaaaagcc tcatatcttt cggatccata tcctttcaga tttaattctt cctgaattcc aggactggaa agaatcggct cagtcaccca gatttctccc gcagatgcaa gcgcttgcac tcttgccgca atatttacgc tctgaccgaa gtaatcaagt ctttcgtcgt tgataacggc aagagcagaa ccttcattta aaccgacctt caaaccgatc tcatgtccgt gttccttaaa ttcctcgttc atccgatcga tacgaaacat catttctaat gaagcaaata gtccttccag cggactagaa aaggtcgcca taaccgcgtc tcccatcgtt tttacgatgg cgccgttaaa cttctttacg gtttcggcga gcaaacggaa atgttcctga accaatcgat atgcaaggat atcccccgct ttatcgtata tttcggtcga gcctctcaaa tccgtaaaaa ggatcgtaag actttttacg tttaaattca attgactgct caatcgttga acgcggaata attcccggaa ggtttgattg ttcaaaagca tcttcgcggt cagaaacggt tggatcaccg tcggatgttc cccgataatt tccaggattc ttttcaagtt tggtttaatg attaaaaagc cggaggttgc gatcgtccgg ttggaaactt tcacctcgta ctcccccggt gaaagatgta attcaacggg agtaaatccg gtaggaagaa gactgaggtc gatgatccga tccttcgtga cttccttaga atcaaaatac aaaaaaacgg ccgaattatt ttccacagaa ctgaattgat acacgggaac gttcgtcgca tccaaatgga ttcgttttgt ctcccccggt tccataacga ttaaatcttg aatattcgaa aaaatgaaat tcagaagctc tttggacttg caaaagttgg cggaaatatg atatctgaga tacgtttcca catccaccaa aggattcaag gattgtttct taagggacgg gttcgccgaa aagctgactt ccacctgatc atccaaagta gtcggcacgt ctatgttaca gatataacaa tgaaaacttt tttcctcgat ttgatccaaa gaagtgtgag aagcggcaac acctccacaa gcggggcaaa tcatgttgta cgcaaaatca agaaaaccga tctttgcgga atgaaggaaa agatcgagcg tctctcctat ttcaaaatca ttctgctttg caaaacgaat cggattaatc cgatggagct gccattcgtc cagaaccctt atgttgtcca taaatcgagc aatggatttt ttcgataaaa ctccgaatcc ttccaaagat tttttctttt gttcgaaaag agtttcattc cacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMW NETLFEQKKK SLEGFGVLSK KSIARFMDNI RVLDEWQLHR INPIRFAKQN DFEIGETLDL FLHSAKIGFL DFAYNMICPA CGGVAASHTS LDQIEEKSFH CYICNIDVPT TLDDQVEVSF SANPSLKKQS LNPLVDVETY LRYHISANFC KSKELLNFIF SNIQDLIVME PGETKRIHLD ATNVPVYQFS SVENNSAVFL YFDSKEVTKD RIIDLSLLPT GFTPVELHLS PGEYEVKVSN RTIATSGFLI IKPNLKRILE IIGEHPTVIQ PFLTAKMLLN NQTFRELFRV QRLSSQLNLN VKSLTILFTD LRGSTEIYDK AGDILAYRLV QEHFRLLAET VKKFNGAIVK TMGDAVMATF SSPLEGLFAS LEMMFRIDRM NEEFKEHGHE IGLKVGLNEG SALAVINDER LDYFGQSVNI AARVQALASA GEIWVTEPIL SSPGIQEELN LKGYGSERYE AFLKGVGQKA AVHKLFKNEE QRAFVGSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgga atgaaactct tttcgaacaa aagaaaaaat ctttggaagg attcggagtt ttatcgaaaa aatccattgc tcgatttatg gacaacataa gggttctgga cgaatggcag ctccatcgga ttaatccgat tcgttttgca aagcagaatg attttgaaat aggagagacg ctcgatcttt tccttcattc cgcaaagatc ggttttcttg attttgcgta caacatgatt tgccccgctt gtggaggtgt tgccgcttct cacacttctt tggatcaaat cgaggaaaaa agttttcatt gttatatctg taacatagac gtgccgacta ctttggatga tcaggtggaa gtcagctttt cggcgaaccc gtcccttaag aaacaatcct tgaatccttt ggtggatgtg gaaacgtatc tcagatatca tatttccgcc aacttttgca agtccaaaga gcttctgaat ttcatttttt cgaatattca agatttaatc gttatggaac cgggggagac aaaacgaatc catttggatg cgacgaacgt tcccgtgtat caattcagtt ctgtggaaaa taattcggcc gtttttttgt attttgattc taaggaagtc acgaaggatc ggatcatcga cctcagtctt cttcctaccg gatttactcc cgttgaatta catctttcac cgggggagta cgaggtgaaa gtttccaacc ggacgatcgc aacctccggc tttttaatca ttaaaccaaa cttgaaaaga atcctggaaa ttatcgggga acatccgacg gtgatccaac cgtttctgac cgcgaagatg cttttgaaca atcaaacctt ccgggaatta ttccgcgttc aacgattgag cagtcaattg aatttaaacg taaaaagtct tacgatcctt tttacggatt tgagaggctc gaccgaaata tacgataaag cgggggatat ccttgcatat cgattggttc aggaacattt ccgtttgctc gccgaaaccg taaagaagtt taacggcgcc atcgtaaaaa cgatgggaga cgcggttatg gcgacctttt ctagtccgct ggaaggacta tttgcttcat tagaaatgat gtttcgtatc gatcggatga acgaggaatt taaggaacac ggacatgaga tcggtttgaa ggtcggttta aatgaaggtt ctgctcttgc cgttatcaac gacgaaagac ttgattactt cggtcagagc gtaaatattg cggcaagagt gcaagcgctt gcatctgcgg gagaaatctg ggtgactgag ccgattcttt ccagtcctgg aattcaggaa gaattaaatc tgaaaggata tggatccgaa agatatgagg cttttttgaa aggagtcggt caaaaagccg ccgttcacaa actgtttaag aacgaggaac aaagggcgtt tgtagggtcg gtttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LpboA.19552.a.B1.PS38489
PURIFICATION DATe: 8/14/2018
CONCENTRATION: 13.46mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMW NETLFEQKKK SLEGFGVLSK KSIARFMDNI RVLDEWQLHR INPIRFAKQN DFEIGETLDL FLHSAKIGFL DFAYNMICPA CGGVAASHTS LDQIEEKSFH CYICNIDVPT TLDDQVEVSF SANPSLKKQS LNPLVDVETY LRYHISANFC KSKELLNFIF SNIQDLIVME PGETKRIHLD ATNVPVYQFS SVENNSAVFL YFDSKEVTKD RIIDLSLLPT GFTPVELHLS PGEYEVKVSN RTIATSGFLI IKPNLKRILE IIGEHPTVIQ PFLTAKMLLN NQTFRELFRV QRLSSQLNLN VKSLTILFTD LRGSTEIYDK AGDILAYRLV QEHFRLLAET VKKFNGAIVK TMGDAVMATF SSPLEGLFAS LEMMFRIDRM NEEFKEHGHE IGLKVGLNEG SALAVINDER LDYFGQSVNI AARVQALASA GEIWVTEPIL SSPGIQEELN LKGYGSERYE AFLKGVGQKA AVHKLFKNE
Validated NT Sequence
tcctcgttct taaacagttt gtgaacggcg gctttttgac cgactccttt caaaaaagcc tcatatcttt cggatccata tcctttcaga tttaattctt cctgaattcc aggactggaa agaatcggct cagtcaccca gatttctccc gcagatgcaa gcgcttgcac tcttgccgca atatttacgc tctgaccgaa gtaatcaagt ctttcgtcgt tgataacggc aagagcagaa ccttcattta aaccgacctt caaaccgatc tcatgtccgt gttccttaaa ttcctcgttc atccgatcga tacgaaacat catttctaat gaagcaaata gtccttccag cggactagaa aaggtcgcca taaccgcgtc tcccatcgtt tttacgatgg cgccgttaaa cttctttacg gtttcggcga gcaaacggaa atgttcctga accaatcgat atgcaaggat atcccccgct ttatcgtata tttcggtcga gcctctcaaa tccgtaaaaa ggatcgtaag actttttacg tttaaattca attgactgct caatcgttga acgcggaata attcccggaa ggtttgattg ttcaaaagca tcttcgcggt cagaaacggt tggatcaccg tcggatgttc cccgataatt tccaggattc ttttcaagtt tggtttaatg attaaaaagc cggaggttgc gatcgtccgg ttggaaactt tcacctcgta ctcccccggt gaaagatgta attcaacggg agtaaatccg gtaggaagaa gactgaggtc gatgatccga tccttcgtga cttccttaga atcaaaatac aaaaaaacgg ccgaattatt ttccacagaa ctgaattgat acacgggaac gttcgtcgca tccaaatgga ttcgttttgt ctcccccggt tccataacga ttaaatcttg aatattcgaa aaaatgaaat tcagaagctc tttggacttg caaaagttgg cggaaatatg atatctgaga tacgtttcca catccaccaa aggattcaag gattgtttct taagggacgg gttcgccgaa aagctgactt ccacctgatc atccaaagta gtcggcacgt ctatgttaca gatataacaa tgaaaacttt tttcctcgat ttgatccaaa gaagtgtgag aagcggcaac acctccacaa gcggggcaaa tcatgttgta cgcaaaatca agaaaaccga tctttgcgga atgaaggaaa agatcgagcg tctctcctat ttcaaaatca ttctgctttg caaaacgaat cggattaatc cgatggagct gccattcgtc cagaaccctt atgttgtcca taaatcgagc aatggatttt ttcgataaaa ctccgaatcc ttccaaagat tttttctttt gttcgaaaag agtttcattc cacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMW NETLFEQKKK SLEGFGVLSK KSIARFMDNI RVLDEWQLHR INPIRFAKQN DFEIGETLDL FLHSAKIGFL DFAYNMICPA CGGVAASHTS LDQIEEKSFH CYICNIDVPT TLDDQVEVSF SANPSLKKQS LNPLVDVETY LRYHISANFC KSKELLNFIF SNIQDLIVME PGETKRIHLD ATNVPVYQFS SVENNSAVFL YFDSKEVTKD RIIDLSLLPT GFTPVELHLS PGEYEVKVSN RTIATSGFLI IKPNLKRILE IIGEHPTVIQ PFLTAKMLLN NQTFRELFRV QRLSSQLNLN VKSLTILFTD LRGSTEIYDK AGDILAYRLV QEHFRLLAET VKKFNGAIVK TMGDAVMATF SSPLEGLFAS LEMMFRIDRM NEEFKEHGHE IGLKVGLNEG SALAVINDER LDYFGQSVNI AARVQALASA GEIWVTEPIL SSPGIQEELN LKGYGSERYE AFLKGVGQKA AVHKLFKNEE QRAFVGSV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGGA ATGAAACTCT TTTCGAACAA AAGAAAAAAT CTTTGGAAGG ATTCGGAGTT TTATCGAAAA AATCCATTGC TCGATTTATG GACAACATAA GGGTTCTGGA CGAATGGCAG CTCCATCGGA TTAATCCGAT TCGTTTTGCA AAGCAGAATG ATTTTGAAAT AGGAGAGACG CTCGATCTTT TCCTTCATTC CGCAAAGATC GGTTTTCTTG ATTTTGCGTA CAACATGATT TGCCCCGCTT GTGGAGGTGT TGCCGCTTCT CACACTTCTT TGGATCAAAT CGAGGAAAAA AGTTTTCATT GTTATATCTG TAACATAGAC GTGCCGACTA CTTTGGATGA TCAGGTGGAA GTCAGCTTTT CGGCGAACCC GTCCCTTAAG AAACAATCCT TGAATCCTTT GGTGGATGTG GAAACGTATC TCAGATATCA TATTTCCGCC AACTTTTGCA AGTCCAAAGA GCTTCTGAAT TTCATTTTTT CGAATATTCA AGATTTAATC GTTATGGAAC CGGGGGAGAC AAAACGAATC CATTTGGATG CGACGAACGT TCCCGTGTAT CAATTCAGTT CTGTGGAAAA TAATTCGGCC GTTTTTTTGT ATTTTGATTC TAAGGAAGTC ACGAAGGATC GGATCATCGA CCTCAGTCTT CTTCCTACCG GATTTACTCC CGTTGAATTA CATCTTTCAC CGGGGGAGTA CGAGGTGAAA GTTTCCAACC GGACGATCGC AACCTCCGGC TTTTTAATCA TTAAACCAAA CTTGAAAAGA ATCCTGGAAA TTATCGGGGA ACATCCGACG GTGATCCAAC CGTTTCTGAC CGCGAAGATG CTTTTGAACA ATCAAACCTT CCGGGAATTA TTCCGCGTTC AACGATTGAG CAGTCAATTG AATTTAAACG TAAAAAGTCT TACGATCCTT TTTACGGATT TGAGAGGCTC GACCGAAATA TACGATAAAG CGGGGGATAT CCTTGCATAT CGATTGGTTC AGGAACATTT CCGTTTGCTC GCCGAAACCG TAAAGAAGTT TAACGGCGCC ATCGTAAAAA CGATGGGAGA CGCGGTTATG GCGACCTTTT CTAGTCCGCT GGAAGGACTA TTTGCTTCAT TAGAAATGAT GTTTCGTATC GATCGGATGA ACGAGGAATT TAAGGAACAC GGACATGAGA TCGGTTTGAA GGTCGGTTTA AATGAAGGTT CTGCTCTTGC CGTTATCAAC GACGAAAGAC TTGATTACTT CGGTCAGAGC GTAAATATTG CGGCAAGAGT GCAAGCGCTT GCATCTGCGG GAGAAATCTG GGTGACTGAG CCGATTCTTT CCAGTCCTGG AATTCAGGAA GAATTAAATC TGAAAGGATA TGGATCCGAA AGATATGAGG CTTTTTTGAA AGGAGTCGGT CAAAAAGCCG CCGTTCACAA ACTGTTTAAG AACGAGGAAC AAAGGGCGTT TGTAGGGTCG GTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT