SSGCID: Target Page

LpboA.19393.a

Sensor histidine kinase of a two component response regulator

CENTER ID:	LpboA.19393.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19393.a.B1.GE41868	full length		1	372

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.3660
UniProt:	Q04VT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENTQDAILS VGKDYKILTM NRLFQEFVFN IYKIDLKIGN NALERFGLDT SATWKDIYDS TFDGIPVRKE WKISNQEGNS NYYEILTSPI YSSTENTRIS YPKDQASFSA TLCNEESKSE REILGATLYI RDITEKKLLE QKTASLIKSK NRLLTTVVHD LKNPINGILG LIELLKEQTD NQNRTELLNM MFRAADKSLN IIQDLLQIAE MENENFKLKT EKTNLNYLIE SLVKQNYPEA QNKKIKLYTI QEIDPLPVKI ELLKFQRVLE NLISNSLKFT KEGGEILIHS YAQNKKAMMI VKDTGIGIPY GLQSSIFDQF TKARRQGLKG EETTGLGMFI VKVIVELHKG KISLESEEGL GTKFIVEIPL DA

NT Sequence

atggaaaata cgcaggacgc gatcttgtcc gtgggcaaag attacaaaat tctaacgatg aatcgcctct ttcaagaatt cgtattcaat atatataaaa tagatctgaa aataggaaat aacgccttag aacggttcgg gctcgatacc tccgccactt ggaaggatat ttacgattcc actttcgacg gcattccggt tcgaaaagaa tggaagattt cgaatcaaga aggaaattca aattattatg aaattctaac ttctcccatt tacagttcga ctgaaaacac tcgaatatcg tatccgaaag atcaggcgtc tttttcggct actctatgca atgaagaatc aaaaagcgag agggaaattc taggagcgac gctttatatt cgagatatca ccgaaaaaaa gttattggaa caaaaaaccg ccagtcttat caaaagtaag aaccggttgt tgacgacggt ggtgcatgat cttaaaaatc cgattaacgg aattctgggc ctaattgaac ttttaaaaga acaaaccgat aatcaaaaca gaacggaact tcttaatatg atgtttcgag cggcggataa atccttaaat atcatccaag atctgttgca gattgcggaa atggaaaatg aaaacttcaa actcaaaacg gaaaaaacga atttaaacta tcttattgaa tcgctcgtaa aacaaaatta ccctgaagct caaaataaaa agatcaaact ttacacaata caggaaatcg atccacttcc ggtaaagata gagttgttga aattccaaag agttttggaa aacctaattt ccaattcatt aaagttcacg aaagaaggcg gagaaatcct cattcattct tacgctcaaa ataaaaaagc gatgatgata gtgaaagaca ccgggatcgg aattccttac ggccttcagt cctcgatttt cgatcagttc acaaaagcca gaagacaagg actaaaaggg gaagaaacta cagggctcgg aatgttcatc gtaaaagtta tcgtggaact tcacaaagga aaaattagtt tagaaagtga agaagggctc ggaactaaat tcattgtaga aattccattg gatgcttga

Details for LpboA.19393.a.B1.GE41868

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHME NTQDAILSVG KDYKILTMNR LFQEFVFNIY KIDLKIGNNA LERFGLDTSA TWKDIYDSTF DGIPVRKEWK ISNQEGNSNY YEILTSPIYS STENTRISYP KDQASFSATL CNEESKSERE ILGATLYIRD ITEKKLLEQK TASLIKSKNR LLTTVVHDLK NPINGILGLI ELLKEQTDNQ NRTELLNMMF RAADKSLNII QDLLQIAEME NENFKLKTEK TNLNYLIESL VKQNYPEAQN KKIKLYTIQE IDPLPVKIEL LKFQRVLENL ISNSLKFTKE GGEILIHSYA QNKKAMMIVK DTGIGIPYGL QSSIFDQFTK ARRQGLKGEE TTGLGMFIVK VIVELHKGKI SLESEEGLGT

Validated NT Sequence

tttagttccg agcccttctt cactttctaa actaattttt cctttgtgaa gttccacgat aacttttacg atgaacattc cgagccctgt agtttcttcc ccttttagtc cttgtcttct ggcttttgtg aactgatcga aaatcgagga ctgaaggccg taaggaattc cgatcccggt gtctttcact atcatcatcg cttttttatt ttgagcgtaa gaatgaatga ggatttctcc gccttctttc gtgaacttta atgaattgga aattaggttt tccaaaactc tttggaattt caacaactct atctttaccg gaagtggatc gatttcctgt attgtgtaaa gtttgatctt tttattttga gcttcagggt aattttgttt tacgagcgat tcaataagat agtttaaatt cgttttttcc gttttgagtt tgaagttttc attttccatt tccgcaatct gcaacagatc ttggatgata tttaaggatt tatccgccgc tcgaaacatc atattaagaa gttccgttct gttttgatta tcggtttgtt cttttaaaag ttcaattagg cccagaattc cgttaatcgg atttttaaga tcatgcacca ccgtcgtcaa caaccggttc ttacttttga taagactggc ggttttttgt tccaataact ttttttcggt gatatctcga atataaagcg tcgctcctag aatttccctc tcgctttttg attcttcatt gcatagagta gccgaaaaag acgcctgatc tttcggatac gatattcgag tgttttcagt cgaactgtaa atgggagaag ttagaatttc ataataattt gaatttcctt cttgattcga aatcttccat tcttttcgaa ccggaatgcc gtcgaaagtg gaatcgtaaa tatccttcca agtggcggag gtatcgagcc cgaaccgttc taaggcgtta tttcctattt tcagatctat tttatatata ttgaatacga attcttgaaa gaggcgattc atcgttagaa ttttgtaatc tttgcccacg gacaagatcg cgtcctgcgt attttccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa atacgcagga cgcgatcttg tccgtgggca aagattacaa aattctaacg atgaatcgcc tctttcaaga attcgtattc aatatatata aaatagatct gaaaatagga aataacgcct tagaacggtt cgggctcgat acctccgcca cttggaagga tatttacgat tccactttcg acggcattcc ggttcgaaaa gaatggaaga tttcgaatca agaaggaaat tcaaattatt atgaaattct aacttctccc atttacagtt cgactgaaaa cactcgaata tcgtatccga aagatcaggc gtctttttcg gctactctat gcaatgaaga atcaaaaagc gagagggaaa ttctaggagc gacgctttat attcgagata tcaccgaaaa aaagttattg gaacaaaaaa ccgccagtct tatcaaaagt aagaaccggt tgttgacgac ggtggtgcat gatcttaaaa atccgattaa cggaattctg ggcctaattg aacttttaaa agaacaaacc gataatcaaa acagaacgga acttcttaat atgatgtttc gagcggcgga taaatcctta aatatcatcc aagatctgtt gcagattgcg gaaatggaaa atgaaaactt caaactcaaa acggaaaaaa cgaatttaaa ctatcttatt gaatcgctcg taaaacaaaa ttaccctgaa gctcaaaata aaaagatcaa actttacaca atacaggaaa tcgatccact tccggtaaag atagagttgt tgaaattcca aagagttttg gaaaacctaa tttccaattc attaaagttc acgaaagaag gcggagaaat cctcattcat tcttacgctc aaaataaaaa agcgatgatg atagtgaaag acaccgggat cggaattcct tacggccttc agtcctcgat tttcgatcag ttcacaaaag ccagaagaca aggactaaaa ggggaagaaa ctacagggct cggaatgttc atcgtaaaag ttatcgtgga acttcacaaa ggaaaaatta gtttagaaag tgaagaaggg ctcggaacta aattcattgt agaaattcca ttggatgctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat