LpboA.19148.a
Glycosyltransferase
| CENTER ID: | LpboA.19148.a |
| ORGANISM: | Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19148.a.B1.GE41867 | full length | 1 | 220 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.19148.a.B1.PS38508 | full length | 1 | 220 |
Structures
6P61
DEPOSITED: 5/31/2019
DETERMINATION: XRay
CLONE: LpboA.19148.a.B1.GE41867
PROTEIN: LpboA.19148.a.B1.PS38508
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_2687 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|355276.3.peg.1508 |
| UniProt: | Q04TP4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKAPTVSVI VAAFNQEKYI GRCIRSLLNQ NFPKEDYEII IINDGSTDKT KYALEIFGKE IKVIENESNK GLSASLNLGI RSALGQFIVR VDADDYVNSD YVSILHKFLS YNPNFDAVAC DYYLVDDHED FLSRKNCMID PIGCGIMFRI EQLIDIGLYD DGFLSHEDKD LRIRFEKKYS IHRVELPLYR YRRHDSNMTN DSELMDERMK SLREKHKDQP
NT Sequence
atgaaaaaag ctccaacagt ttccgttatc gtcgctgcat ttaatcaaga gaagtatatt ggaagatgta ttcgctctct attgaatcaa aattttccaa aagaagatta tgaaattatt ataatcaatg atggaagtac ggataagacg aaatatgccc ttgaaatctt cggcaaagaa ataaaagtaa ttgaaaacga gagtaataag ggtttgtcag catctttaaa tcttggaatc agatctgcgt taggacagtt tatcgttagg gtcgatgccg atgattatgt gaattcagat tatgttagta ttcttcataa atttctatct tataatccaa atttcgatgc agttgcttgt gattattatt tggttgacga ccacgaggat tttttgtccc gaaaaaattg tatgatagat ccaattggct gtggaattat gtttcgaatt gaacagctga ttgatattgg attgtatgac gacggatttc tttctcatga agataaagac ttgagaattc gttttgaaaa gaaatattca atacacagag tagaactccc tctttatcgg tatagaaggc atgattcaaa tatgacgaat gactctgaat tgatggacga gcgaatgaaa agtttgaggg aaaaacataa agatcagcct tga
Details for LpboA.19148.a.B1.GE41867
| HARVESTED ON: | 8/31/2017 |
| SEQUENCED ON: | 9/29/2017 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMK KAPTVSVIVA AFNQEKYIGR CIRSLLNQNF PKEDYEIIII NDGSTDKTKY ALEIFGKEIK VIENESNKGL SASLNLGIRS ALGQFIVRVD ADDYVNSDYV SILHKFLSYN PNFDAVACDY YLVDDHEDFL SRKNCMIDPI GCGIMFRIEQ LIDIGLYDDG FLSHEDKDLR IRFEKKYSIH RVELPLYRYR RHDSNMTNDS ELMDERMKSL REKHKDQP
Validated NT Sequence
ttactcactt aaggctgatc tttatgtttt tccctcaaac ttttcattcg ctcgtccatc aattcagagt cattcgtcat atttgaatca tgccttctat accgataaag agggagttct actctgtgta ttgaatattt cttttcaaaa cgaattctca agtctttatc ttcatgagaa agaaatccgt cgtcatacaa tccaatatca atcagctgtt caattcgaaa cataattcca cagccaattg gatctatcat acaatttttt cgggacaaaa aatcctcgtg gtcgtcaacc aaataataat cacaagcaac tgcatcgaaa tttggattat aagatagaaa tttatgaaga atactaacat aatctgaatt cacataatca tcggcatcga ccctaacgat aaactgtcct aacgcagatc tgattccaag atttaaagat gctgacaaac ccttattact ctcgttttca attactttta tttctttgcc gaagatttca agggcatatt tcgtcttatc cgtacttcca tcattgatta taataatttc ataatcttct tttggaaaat tttgattcaa tagagagcga atacatcttc caatatactt ctcttgatta aatgcagcga cgataacgga aactgttgga gcttttttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMK KAPTVSVIVA AFNQEKYIGR CIRSLLNQNF PKEDYEIIII NDGSTDKTKY ALEIFGKEIK VIENESNKGL SASLNLGIRS ALGQFIVRVD ADDYVNSDYV SILHKFLSYN PNFDAVACDY YLVDDHEDFL SRKNCMIDPI GCGIMFRIEQ LIDIGLYDDG FLSHEDKDLR IRFEKKYSIH RVELPLYRYR RHDSNMTNDS ELMDERMKSL REKHKDQP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aagctccaac agtttccgtt atcgtcgctg catttaatca agagaagtat attggaagat gtattcgctc tctattgaat caaaattttc caaaagaaga ttatgaaatt attataatca atgatggaag tacggataag acgaaatatg cccttgaaat cttcggcaaa gaaataaaag taattgaaaa cgagagtaat aagggtttgt cagcatcttt aaatcttgga atcagatctg cgttaggaca gtttatcgtt agggtcgatg ccgatgatta tgtgaattca gattatgtta gtattcttca taaatttcta tcttataatc caaatttcga tgcagttgct tgtgattatt atttggttga cgaccacgag gattttttgt cccgaaaaaa ttgtatgata gatccaattg gctgtggaat tatgtttcga attgaacagc tgattgatat tggattgtat gacgacggat ttctttctca tgaagataaa gacttgagaa ttcgttttga aaagaaatat tcaatacaca gagtagaact ccctctttat cggtatagaa ggcatgattc aaatatgacg aatgactctg aattgatgga cgagcgaatg aaaagtttga gggaaaaaca taaagatcag ccttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LpboA.19148.a.B1.PS38508
| PURIFICATION DATe: | 9/25/2018 |
| CONCENTRATION: | 52.58mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK KAPTVSVIVA AFNQEKYIGR CIRSLLNQNF PKEDYEIIII NDGSTDKTKY ALEIFGKEIK VIENESNKGL SASLNLGIRS ALGQFIVRVD ADDYVNSDYV SILHKFLSYN PNFDAVACDY YLVDDHEDFL SRKNCMIDPI GCGIMFRIEQ LIDIGLYDDG FLSHEDKDLR IRFEKKYSIH RVELPLYRYR RHDSNMTNDS ELMDERMKSL REKHKDQP
Validated NT Sequence
ttactcactt aaggctgatc tttatgtttt tccctcaaac ttttcattcg ctcgtccatc aattcagagt cattcgtcat atttgaatca tgccttctat accgataaag agggagttct actctgtgta ttgaatattt cttttcaaaa cgaattctca agtctttatc ttcatgagaa agaaatccgt cgtcatacaa tccaatatca atcagctgtt caattcgaaa cataattcca cagccaattg gatctatcat acaatttttt cgggacaaaa aatcctcgtg gtcgtcaacc aaataataat cacaagcaac tgcatcgaaa tttggattat aagatagaaa tttatgaaga atactaacat aatctgaatt cacataatca tcggcatcga ccctaacgat aaactgtcct aacgcagatc tgattccaag atttaaagat gctgacaaac ccttattact ctcgttttca attactttta tttctttgcc gaagatttca agggcatatt tcgtcttatc cgtacttcca tcattgatta taataatttc ataatcttct tttggaaaat tttgattcaa tagagagcga atacatcttc caatatactt ctcttgatta aatgcagcga cgataacgga aactgttgga gcttttttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMK KAPTVSVIVA AFNQEKYIGR CIRSLLNQNF PKEDYEIIII NDGSTDKTKY ALEIFGKEIK VIENESNKGL SASLNLGIRS ALGQFIVRVD ADDYVNSDYV SILHKFLSYN PNFDAVACDY YLVDDHEDFL SRKNCMIDPI GCGIMFRIEQ LIDIGLYDDG FLSHEDKDLR IRFEKKYSIH RVELPLYRYR RHDSNMTNDS ELMDERMKSL REKHKDQP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA AAGCTCCAAC AGTTTCCGTT ATCGTCGCTG CATTTAATCA AGAGAAGTAT ATTGGAAGAT GTATTCGCTC TCTATTGAAT CAAAATTTTC CAAAAGAAGA TTATGAAATT ATTATAATCA ATGATGGAAG TACGGATAAG ACGAAATATG CCCTTGAAAT CTTCGGCAAA GAAATAAAAG TAATTGAAAA CGAGAGTAAT AAGGGTTTGT CAGCATCTTT AAATCTTGGA ATCAGATCTG CGTTAGGACA GTTTATCGTT AGGGTCGATG CCGATGATTA TGTGAATTCA GATTATGTTA GTATTCTTCA TAAATTTCTA TCTTATAATC CAAATTTCGA TGCAGTTGCT TGTGATTATT ATTTGGTTGA CGACCACGAG GATTTTTTGT CCCGAAAAAA TTGTATGATA GATCCAATTG GCTGTGGAAT TATGTTTCGA ATTGAACAGC TGATTGATAT TGGATTGTAT GACGACGGAT TTCTTTCTCA TGAAGATAAA GACTTGAGAA TTCGTTTTGA AAAGAAATAT TCAATACACA GAGTAGAACT CCCTCTTTAT CGGTATAGAA GGCATGATTC AAATATGACG AATGACTCTG AATTGATGGA CGAGCGAATG AAAAGTTTGA GGGAAAAACA TAAAGATCAG CCTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT