LpboA.18935.a

Prohibitin family protein

CENTER ID: LpboA.18935.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.18935.a.B2.GE41865 soluble domain 57 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.377
UniProt: Q04PJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSDFFSRLK KIVVSFRTSI FVSSKGKIMK KVLEFIKEHW IKILGLILVF IFNPLVCIGT GHRGVVTNLG SVSDRILGEG INFITPVVQS VKSIDVRIQK VEANSTAPSS DLQGIHTMIT LTYHLSPNQV NKLYQEIGMD YEDTIIVPAI LETMKHVTAQ FTASDLVTKR ESVSLKIHEL LHTKLGKFYI LVDEVSMKDF EFSKTFSESI ELKQKAEQDA LRAKNELERV KIEAEQQIVN ARAEAETLRL KSQQITPMMA RWSGLKSGTA NIPIRIWEAV PTRFFN
NT Sequence
atgttatcgg attttttcag tcgtcttaaa aaaatcgtcg tatcttttag aactagtata ttcgtatctt ccaaagggaa aattatgaaa aaagttttag aatttattaa agaacattgg atcaagattt taggattgat tttggttttt atcttcaatc cgctcgtttg tatcggaacg ggacacagag gggttgttac gaatctgggc tccgtttccg atcggattct cggagaagga atcaatttca ttacgccggt cgttcaatcc gttaagagca tagacgtaag aattcaaaaa gtggaagcta attcgacggc tccttccagc gatttgcagg ggattcatac tatgattacg ttgacgtatc atctttctcc gaaccaggtc aataaacttt atcaggaaat cggaatggac tatgaagata cgattattgt tcccgcaatt cttgaaacga tgaaacacgt gaccgcgcag tttaccgctt cggaccttgt cacaaaaagg gaaagtgttt ccttaaagat ccacgaattg ctgcacacga aactcggtaa gttttatatt ctcgtggacg aagtgtcaat gaaggatttt gaattttcca aaacgttttc cgaatcgatc gaactaaaac aaaaggcgga acaggacgcg cttcgggcca aaaacgaatt ggaacgagtt aagatcgagg cggaacaaca gatcgtaaat gcaagggcgg aagcggaaac attaagactg aaatcccaac aaattactcc gatgatggcg agatggagcg gattaaaaag tggaacggca aatatcccga tacgtatttg ggaagcggtt ccaacacgct ttttcaatta a
Details for LpboA.18935.a.B2.GE41865
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC IGTGHRGVVT NLGSVSDRIL GEGINFITPV VQSVKSIDVR IQKVEANSTA PSSDLQGIHT MITLTYHLSP NQVNKLYQEI GMDYEDTIIV PAILETMKHV TAQFTASDLV TKRESVSLKI HELLHTKLGK FYILVDEVSM KDFEFSKTFS ESIELKQKAE QDALRAKNEL ERVKIEAEQQ IVNARAEAET LRLKSQQITP MMARWSGLKS GTANIPIRIW EAVPTRFFN
Validated NT Sequence
atcttactca cttaattgaa aaagcgtgtt ggaaccgctt cccaaatacg tatcgggata tttgccgttc cactttttaa tccgctccat ctcgccatca tcggagtaat ttgttgggat ttcagtctta atgtttccgc ttccgccctt gcatttacga tctgttgttc cgcctcgatc ttaactcgtt ccaattcgtt tttggcccga agcgcgtcct gttccgcctt ttgttttagt tcgatcgatt cggaaaacgt tttggaaaat tcaaaatcct tcattgacac ttcgtccacg agaatataaa acttaccgag tttcgtgtgc agcaattcgt ggatctttaa ggaaacactt tccctttttg tgacaaggtc cgaagcggta aactgcgcgg tcacgtgttt catcgtttca agaattgcgg gaacaataat cgtatcttca tagtccattc cgatttcctg ataaagttta ttgacctggt tcggagaaag atgatacgtc aacgtaatca tagtatgaat cccctgcaaa tcgctggaag gagccgtcga attagcttcc actttttgaa ttcttacgtc tatgctctta acggattgaa cgaccggcgt aatgaaattg attccttctc cgagaatccg atcggaaacg gagcccagat tcgtaacaac ccctctgtgt cccgttccga tacacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHCI GTGHRGVVTN LGSVSDRILG EGINFITPVV QSVKSIDVRI QKVEANSTAP SSDLQGIHTM ITLTYHLSPN QVNKLYQEIG MDYEDTIIVP AILETMKHVT AQFTASDLVT KRESVSLKIH ELLHTKLGKF YILVDEVSMK DFEFSKTFSE SIELKQKAEQ DALRAKNELE RVKIEAEQQI VNARAEAETL RLKSQQITPM MARWSGLKSG TANIPIRIWE AVPTRFFN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgtatcg gaacgggaca cagaggggtt gttacgaatc tgggctccgt ttccgatcgg attctcggag aaggaatcaa tttcattacg ccggtcgttc aatccgttaa gagcatagac gtaagaattc aaaaagtgga agctaattcg acggctcctt ccagcgattt gcaggggatt catactatga ttacgttgac gtatcatctt tctccgaacc aggtcaataa actttatcag gaaatcggaa tggactatga agatacgatt attgttcccg caattcttga aacgatgaaa cacgtgaccg cgcagtttac cgcttcggac cttgtcacaa aaagggaaag tgtttcctta aagatccacg aattgctgca cacgaaactc ggtaagtttt atattctcgt ggacgaagtg tcaatgaagg attttgaatt ttccaaaacg ttttccgaat cgatcgaact aaaacaaaag gcggaacagg acgcgcttcg ggccaaaaac gaattggaac gagttaagat cgaggcggaa caacagatcg taaatgcaag ggcggaagcg gaaacattaa gactgaaatc ccaacaaatt actccgatga tggcgagatg gagcggatta aaaagtggaa cggcaaatat cccgatacgt atttgggaag cggttccaac acgctttttc aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat