LpboA.18779.a

Uncharacterized protein

CENTER ID: LpboA.18779.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.18779.a.B1.GE41862 full length 1 227
LpboA.18779.a.B2.GE42119 PF08889_0 DUF 7 221
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1530
UniProt: Q04TM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGVILQPNY IPWRGYFELI GKADVFVFLD DVQYTNRDWR NRNQIKTQTG LQWLTIPVFQ TSKFGQLIHE VEIDNSSKWY EKHLNAITRN YSKASFFKKF DELLEILYSG KLSSLSDLDI LSIEWIARYL ELNTKFIRAS QIANEGTRQE KLISICKEVG ITTYISGPSA KSYIVPENFE KDGISLLYQE YNYPNYPQLY GDFVGNVSIL DLLFNCGFES RKYIFKE
NT Sequence
atgattggtg ttatactcca accaaattat atcccttgga ggggttattt cgagttgatt ggtaaagcag acgttttcgt atttttagac gacgttcaat atacaaacag agattggaga aatagaaatc aaataaaaac acaaacaggc cttcagtggt tgacaattcc agtatttcaa acgagtaaat tcggacaatt gatccacgag gtagaaatcg ataactcttc gaaatggtat gaaaaacatc taaatgcaat aacacgtaat tattcgaagg catctttttt taagaaattt gatgaactat tagaaatact ttattcaggg aagttgagta gtctttctga cttagatatc ctatctattg agtggattgc aagatacttg gaactaaata caaaatttat acgtgcatcg cagattgcta atgaagggac tcgacaagaa aaattgatca gtatttgtaa agaagtagga attacgacct atatctctgg gcctagtgcc aaaagctata ttgttcctga aaattttgaa aaggatggga tttctttact ctatcaggaa tataattatc cgaactatcc tcagttatat ggtgattttg tcggtaatgt tagtatatta gatcttttat ttaactgcgg ttttgaaagc cgtaaatata tatttaagga ataa
Details for LpboA.18779.a.B1.GE41862
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI GVILQPNYIP WRGYFELIGK ADVFVFLDDV QYTNRDWRNR NQIKTQTGLQ WLTIPVFQTS KFGQLIHEVE IDNSSKWYEK HLNAITRNYS KASFFKKFDE LLEILYSGKL SSLSDLDILS IEWIARYLEL NTKFIRASQI ANEGTRQEKL ISICKEVGIT TYISGPSAKS YIVPENFEKD GISLLYQEYN YPNYPQLYGD FVGNVSILDL LFNCGFESRK YIFKE
Validated NT Sequence
atcttactca cttattcctt aaatatatat ttacggcttt caaaaccgca gttaaataaa agatctaata tactaacatt accgacaaaa tcaccatata actgaggata gttcggataa ttatattcct gatagagtaa agaaatccca tccttttcaa aattttcagg aacaatatag cttttggcac taggcccaga gatataggtc gtaattccta cttctttaca aatactgatc aatttttctt gtcgagtccc ttcattagca atctgcgatg cacgtataaa ttttgtattt agttccaagt atcttgcaat ccactcaata gataggatat ctaagtcaga aagactactc aacttccctg aataaagtat ttctaatagt tcatcaaatt tcttaaaaaa agatgccttc gaataattac gtgttattgc atttagatgt ttttcatacc atttcgaaga gttatcgatt tctacctcgt ggatcaattg tccgaattta ctcgtttgaa atactggaat tgtcaaccac tgaaggcctg tttgtgtttt tatttgattt ctatttctcc aatctctgtt tgtatattga acgtcgtcta aaaatacgaa aacgtctgct ttaccaatca actcgaaata acccctccaa gggatataat ttggttggag tataacacca atcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMI GVILQPNYIP WRGYFELIGK ADVFVFLDDV QYTNRDWRNR NQIKTQTGLQ WLTIPVFQTS KFGQLIHEVE IDNSSKWYEK HLNAITRNYS KASFFKKFDE LLEILYSGKL SSLSDLDILS IEWIARYLEL NTKFIRASQI ANEGTRQEKL ISICKEVGIT TYISGPSAKS YIVPENFEKD GISLLYQEYN YPNYPQLYGD FVGNVSILDL LFNCGFESRK YIFKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg gtgttatact ccaaccaaat tatatccctt ggaggggtta tttcgagttg attggtaaag cagacgtttt cgtattttta gacgacgttc aatatacaaa cagagattgg agaaatagaa atcaaataaa aacacaaaca ggccttcagt ggttgacaat tccagtattt caaacgagta aattcggaca attgatccac gaggtagaaa tcgataactc ttcgaaatgg tatgaaaaac atctaaatgc aataacacgt aattattcga aggcatcttt ttttaagaaa tttgatgaac tattagaaat actttattca gggaagttga gtagtctttc tgacttagat atcctatcta ttgagtggat tgcaagatac ttggaactaa atacaaaatt tatacgtgca tcgcagattg ctaatgaagg gactcgacaa gaaaaattga tcagtatttg taaagaagta ggaattacga cctatatctc tgggcctagt gccaaaagct atattgttcc tgaaaatttt gaaaaggatg ggatttcttt actctatcag gaatataatt atccgaacta tcctcagtta tatggtgatt ttgtcggtaa tgttagtata ttagatcttt tatttaactg cggttttgaa agccgtaaat atatatttaa ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LpboA.18779.a.B2.GE42119
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 26kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ PNYIPWRGYF ELIGKADVFV FLDDVQYTNR DWRNRNQIKT QTGLQWLTIP VFQTSKFGQL IHEVEIDNSS KWYEKHLNAI TRNYSKASFF KKFDELLEIL YSGKLSSLSD LDILSIEWIA RYLELNTKFI RASQIANEGT RQEKLISICK EVGITTYISG PSAKSYIVPE NFEKDGISLL YQEYNYPNYP QLYGDFVGNV SILDLLFNCG FESR
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa ccaaattata tcccttggag gggttatttc gagttgattg gtaaagcaga cgttttcgta tttttagacg acgttcaata tacaaacaga gattggagaa atagaaatca aataaaaaca caaacaggcc ttcagtggtt gacaattcca gtatttcaaa cgagtaaatt cggacaattg atccacgagg tagaaatcga taactcttcg aaatggtatg aaaaacatct aaatgcaata acacgtaatt attcgaaggc atcttttttt aagaaatttg atgaactatt agaaatactt tattcaggga agttgagtag tctttctgac ttagatatcc tatctattga gtggattgca agatacttgg aactaaatac aaaatttata cgtgcatcgc agattgctaa tgaagggact cgacaagaaa aattgatcag tatttgtaaa gaagtaggaa ttacgaccta tatctctggg cctagtgcca aaagctatat tgttcctgaa aattttgaaa aggatgggat ttctttactc tatcaggaat ataattatcc gaactatcct cagttatatg gtgattttgt cggtaatgtt agtatattag atcttttatt taactgcggt tttgaaagcc gttaagtgag taagat
Expected Protein Sequence
MAHHHHHHQP NYIPWRGYFE LIGKADVFVF LDDVQYTNRD WRNRNQIKTQ TGLQWLTIPV FQTSKFGQLI HEVEIDNSSK WYEKHLNAIT RNYSKASFFK KFDELLEILY SGKLSSLSDL DILSIEWIAR YLELNTKFIR ASQIANEGTR QEKLISICKE VGITTYISGP SAKSYIVPEN FEKDGISLLY QEYNYPNYPQ LYGDFVGNVS ILDLLFNCGF ESR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaccaa attatatccc ttggaggggt tatttcgagt tgattggtaa agcagacgtt ttcgtatttt tagacgacgt tcaatataca aacagagatt ggagaaatag aaatcaaata aaaacacaaa caggccttca gtggttgaca attccagtat ttcaaacgag taaattcgga caattgatcc acgaggtaga aatcgataac tcttcgaaat ggtatgaaaa acatctaaat gcaataacac gtaattattc gaaggcatct ttttttaaga aatttgatga actattagaa atactttatt cagggaagtt gagtagtctt tctgacttag atatcctatc tattgagtgg attgcaagat acttggaact aaatacaaaa tttatacgtg catcgcagat tgctaatgaa gggactcgac aagaaaaatt gatcagtatt tgtaaagaag taggaattac gacctatatc tctgggccta gtgccaaaag ctatattgtt cctgaaaatt ttgaaaagga tgggatttct ttactctatc aggaatataa ttatccgaac tatcctcagt tatatggtga ttttgtcggt aatgttagta tattagatct tttatttaac tgcggttttg aaagccgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat