SSGCID: Target Page

LpboA.18694.a

Cation/multidrug efflux pump

CENTER ID:	LpboA.18694.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.18694.a.B2.GE41860	soluble domain #1		35	330
LpboA.18694.a.B3.GE41861	soluble domain #2		587	883

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.18694.a.B2.PS38497	soluble domain #1		35	330

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.2681
UniProt:	Q04R76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSLIGLLYR RHITTFMLFV AVGIFGLISI PRLPISLMPP SNSPAITILT RYPGISPSKI EEIITKPIEE QISGVGGIED ILSSSEEGES RINVIFKPGT KISYRSLEVR SKIDLIRGNF PREVEEPAIL RYDPTNKPIF IVKLESERYK LKELREFGEN KYKKKFERID GVSEIHVGGG FQREINIDID KGKLLQTGVG LEEVLSKIRT ANVDVPAGNL LIGDNYINIR VIGKLIQIKE IGKIPITVGK SEQIVYLSDI SNVKDEFKEK EDIARDAGKE VVSIYVQKAG DANSLALCEE LRKELSSIHI EGIQQSINYD QSQTIKTAIA KVADSAISGG IIAVVVLFLF LRNFYATILV SVSIPLSIIS TFGLMYFFNV GINVISLCGS ALGVGMLVDC SVVVIDRIFF VHQENGFVED GIVKASSRLS KELFASVLTS IAVFFPIFFS SRELRDLYGG LAITVSASLV TSLIVSLTFL PTLAKFILTK ESRFQTLVPP QYVLRYISKM KSYIEVGTYY KILSWLRNKI LRRQTNLNPE SPDPHELLQF DLIKSLKFLL KHYKKTALYS TAIIVVCFCL SYFLKQEYID PTDSNEISAS LEVETGTHLD ATDLITKSVE RVIENNPNVE KVSSKVEKWH SDLMIKLKPA SSREGKSAND VITELKKETD KLKNAFVFYT ESGAGEGSKE IDIEIIGDEA ERIKELARES AKEVGGIPGI QQVALRFKEG KDQITFSIDK VKASQSGLLP NQIANFMKTA YTGSIPTKFY DKDKEIDIRV RFSEEDRKGP ESVFGVAISS SQSEKIAKTP LIELSSIKKE KAESRIYRKN KRRTYGLTAK LGDIDTGSAV IKIETALKKI SFPENYHYEM TGNYKKLKES KTEMFNLIIL AFIIIVCILS SLFESFLLPF LIILTIPFSA AGVILILFVC RMSLNISVYI GFVMLAGITV NNSILIVETF LEKLKSNQYY EIDELLTEGI RERIRPILIT TITTVLGLLP SVLDSGEGSQ LWRPLAVTVV AGLSISTALT VFLFPAFFKI AVPYLLEDHK SIPLQNKIKK RSKKI

NT Sequence

atggattctt taatcggtct attatacaga agacatataa cgacgtttat gctgtttgta gcagtcggga ttttcggatt gatttccatt ccaaggctgc ctatttcgtt aatgcctcca tccaattcgc cggctattac catactgact cgatatcccg gaatttcacc cagtaaaatc gaagaaatca taacgaaacc gatagaagaa caaatttccg gcgtaggcgg aatcgaagat atactatctt cttccgaaga aggggaatca agaatcaacg tcatattcaa acccggcact aaaatctcgt atcgttccct cgaagtacgt tctaaaatcg atctcatacg gggtaacttt ccaagagaag tggaagaacc cgccattctt agatacgatc ctaccaacaa accgattttc atcgtaaaac tcgaatccga aagatacaaa ttgaaggaac tgagagaatt cggcgaaaac aaatataaaa agaagttcga aagaatcgat ggagtaagtg aaattcacgt cggaggtgga tttcaaagag agataaacat agatatagac aaaggtaagt tactgcagac cggagtcggg ttagaagaag tcttatccaa gatcagaact gcaaacgtag acgttcccgc cggaaattta ttgatcggag acaactatat caacattcgg gtgatcggta agttgattca aattaaagag atcggaaaaa taccgattac ggtcggcaaa tcggaacaaa ttgtttactt atcggacata tcgaatgtga aggacgaatt caaagaaaag gaagatatag caagagacgc aggtaaagaa gtcgtatcca tttacgttca aaaagccggt gatgcaaatt cactagcgtt atgtgaagag ctccgaaagg aactttcctc gattcatata gaaggaattc agcaaagtat caactatgat caatctcaga caatcaaaac ggccatcgca aaagttgcgg attccgctat ttcgggaggt atcatcgcag tcgtcgtttt gtttcttttt ttacggaact tctacgctac aattctcgta tccgtatcga tcccgctttc gatcatttca acgttcgggt tgatgtactt tttcaacgta ggaatcaacg ttatctccct ttgcggatcg gctttaggcg tgggaatgct tgtggattgt tcggtagtgg ttatcgaccg tatatttttt gtacaccagg aaaacggatt cgtagaggat ggtattgtaa aagcctcttc cagattatcg aaggaattgt tcgcgtccgt tttgacgagc attgccgttt tttttccgat ctttttcagt tcccgggagt tacgagatct ctacggaggc ctggcgatta ccgtttctgc gtctttggtc acctctctaa tcgtatccct gacttttttg cccactcttg ccaaatttat tttaacgaaa gagagtcgct ttcaaaccct cgttccaccg cagtatgttc ttaggtatat ttcgaaaatg aaatcttata ttgaagtcgg aacgtactat aagatcttat cttggctccg gaataaaatt ttgcgccgtc aaacgaactt aaatcctgaa tctccggatc cacatgagct tctccaattt gatttaataa aatctttgaa gtttctactc aaacactata aaaaaaccgc tctatactcc acggcgatca tcgtagtttg tttttgcctc tcttattttt tgaagcagga atacattgat ccgaccgatt ccaacgaaat cagcgcttcg ttggaagtag aaacgggaac ccaccttgat gcgactgatt tgatcaccaa gagcgtggaa agagtaatcg aaaataatcc aaacgtggaa aaagtatcct ccaaagttga aaaatggcat tcggatctga tgataaagtt aaaacccgcc tcttctcggg aaggcaaatc agccaatgac gttataaccg aattaaaaaa ggaaacagac aagttaaaga acgcatttgt attttatacc gaatccggtg ccggagaagg atcaaaggaa atcgatattg aaattatagg agacgaagcc gaaaggatca aagagttagc cagagaatct gcaaaagaag taggagggat acccggaatt cagcaggttg cccttcgctt caaggaagga aaagatcaaa tcactttttc gatcgataaa gtgaaggcct cacaatccgg attattacct aatcaaatcg caaactttat gaaaaccgca tatacgggct ctattcccac taaattttac gataaagata aggagatcga cattcgagtc cgattttccg aagaagacag aaaaggtccg gagtcggttt ttggagttgc aatttcctcc tctcaatcgg aaaaaatcgc aaaaacaccc ttgattgaac ttagttcgat caaaaaggaa aaagcagaat ccaggattta tagaaaaaac aaacgaagaa cttatggact cacggctaag ttaggcgata tcgataccgg ttcagccgtt atcaaaattg aaaccgcttt aaagaaaatt tctttcccgg aaaattatca ttacgaaatg accggaaatt acaaaaaact gaaagaaagt aaaaccgaaa tgttcaatct aatcatcctg gccttcatca taatcgtttg cattttatca tctttattcg agtccttttt acttccgttt ctaatcatac tcaccattcc attctcggca gcgggggtaa ttttaattct atttgtgtgt cgcatgtcct taaatatatc cgtatatatc ggtttcgtta tgttagccgg aattaccgta aacaattcga tattaattgt ggaaactttt ttagaaaaat taaaatcaaa tcaatattac gaaatcgacg aattgttaac cgaaggaatc cgagagcgaa ttcgtccgat tctgatcaca accataacca cggtcctagg gctacttcca tccgtcttag attcaggaga aggaagtcaa ctgtggagac cccttgccgt tacggtagtc gcaggattgt ccatttctac ggctcttacg gtatttttat ttcccgcatt ttttaaaatc gcggttcctt atcttctgga agatcataaa agtattccgc tacaaaataa aatcaaaaaa cgttcgaaga aaatataa

Details for LpboA.18694.a.B2.GE41860

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI SLMPPSNSPA ITILTRYPGI SPSKIEEIIT KPIEEQISGV GGIEDILSSS EEGESRINVI FKPGTKISYR SLEVRSKIDL IRGNFPREVE EPAILRYDPT NKPIFIVKLE SERYKLKELR EFGENKYKKK FERIDGVSEI HVGGGFQREI NIDIDKGKLL QTGVGLEEVL SKIRTANVDV PAGNLLIGDN YINIRVIGKL IQIKEIGKIP ITVGKSEQIV YLSDISNVKD EFKEKEDIAR DAGKEVVSIY VQKAGDANSL ALCEELRKEL SSIHIEGIQQ SINYDQSQTI KTAIA

Validated NT Sequence

ttactcactt atgcgatggc cgttttgatt gtctgngatt gatcatagtt gatactttgc tgaattcctt ctatatgaat cgaggaaagt tcctttcgga gctcttcaca taacgctagt gaatttgcat caccggcttt ttgaacgtaa atggatacga cttctttacc tgcgtctctt gctatatctt ccttttcttt gaattcgtcc ttcacattcg atatgtccga taagtaaaca atttgttccg atttgccgac cgtaatcggt atttttccga tctctttaat ttgaatcaac ttaccgatca cccgaatgtt gatatagttg tctccgatca ataaatttcc ggcgggaacg tctacgtttg cagttctgat cttggataag acttcttcta acccgactcc ggtctgcagt aacttacctt tgtctatatc tatgtttatc tctctttgaa atccacctcc gacgtgaatt tcacttactc catcgattct ttcgaacttc tttttatatt tgttttcgcc gaattctctc agttccttca atttgtatct ttcggattcg agttttacga tgaaaatcgg tttgttggta ggatcgtatc taagaatggc gggttcttcc acttctcttg gaaagttacc ccgtatgaga tcgattttag aacgtacttc gagggaacga tacgagattt tagtgccggg tttgaatatg acgttgattc ttgattcccc ttcttcggaa gaagatagta tatcttcgat tccgcctacg ccggaaattt gttcttctat cggtttcgtt atgatttctt cgattttact gggtgaaatt ccgggatatc gagtcagtat ggtaatagcc ggcgaattgg atggaggcat taacgaaatc atatggtggt ggtggtggtg agccatggta t

Expected Protein Sequence

MAHHHHHHIS LMPPSNSPAI TILTRYPGIS PSKIEEIITK PIEEQISGVG GIEDILSSSE EGESRINVIF KPGTKISYRS LEVRSKIDLI RGNFPREVEE PAILRYDPTN KPIFIVKLES ERYKLKELRE FGENKYKKKF ERIDGVSEIH VGGGFQREIN IDIDKGKLLQ TGVGLEEVLS KIRTANVDVP AGNLLIGDNY INIRVIGKLI QIKEIGKIPI TVGKSEQIVY LSDISNVKDE FKEKEDIARD AGKEVVSIYV QKAGDANSLA LCEELRKELS SIHIEGIQQS INYDQSQTIK TAIA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatttcgt taatgcctcc atccaattcg ccggctatta ccatactgac tcgatatccc ggaatttcac ccagtaaaat cgaagaaatc ataacgaaac cgatagaaga acaaatttcc ggcgtaggcg gaatcgaaga tatactatct tcttccgaag aaggggaatc aagaatcaac gtcatattca aacccggcac taaaatctcg tatcgttccc tcgaagtacg ttctaaaatc gatctcatac ggggtaactt tccaagagaa gtggaagaac ccgccattct tagatacgat cctaccaaca aaccgatttt catcgtaaaa ctcgaatccg aaagatacaa attgaaggaa ctgagagaat tcggcgaaaa caaatataaa aagaagttcg aaagaatcga tggagtaagt gaaattcacg tcggaggtgg atttcaaaga gagataaaca tagatataga caaaggtaag ttactgcaga ccggagtcgg gttagaagaa gtcttatcca agatcagaac tgcaaacgta gacgttcccg ccggaaattt attgatcgga gacaactata tcaacattcg ggtgatcggt aagttgattc aaattaaaga gatcggaaaa ataccgatta cggtcggcaa atcggaacaa attgtttact tatcggacat atcgaatgtg aaggacgaat tcaaagaaaa ggaagatata gcaagagacg caggtaaaga agtcgtatcc atttacgttc aaaaagccgg tgatgcaaat tcactagcgt tatgtgaaga gctccgaaag gaactttcct cgattcatat agaaggaatt cagcaaagta tcaactatga tcaatctcag acaatcaaaa cggccatcgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for LpboA.18694.a.B3.GE41861

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHME YIDPTDSNEI SASLEVETGT HLDATDLITK SVERVIENNP NVEKVSSKVE KWHSDLMIKL KPASSREGKS ANDVITELKK ETDKLKNAFV FYTESGAGEG SKEIDIEIIG DEAERIKELA RESAKEVGGI PGIQQVALRF KEGKDQITFS IDKVKASQSG LLPNQIANFM KTAYTGSIPT KFYDKDKEID IRVRFSEEDR KGPESVFGVA ISSSQSEKIA KTPLIELSSI KKEKAESRIY RKNKRRTYGL TAKLGDIDTG SAVIKIETAL KKISFPENYH YEMTGNYKXX XRK

Validated NT Sequence

atcttactca cttattcggt tttactttct ttnnnnnttt ttgtaatttc cggtcatttc gtaatgataa ttttccggga aagaaatttt ctttaaagcg gtttcaattt tgataacggc tgaaccggta tcgatatcgc ctaacttagc cgtgagtcca taagttcttc gtttgttttt tctataaatc ctggattctg ctttttcctt tttgatcgaa ctaagttcaa tcaagggtgt ttttgcgatt ttttccgatt gagaggagga aattgcaact ccaaaaaccg actccggacc ttttctgtct tcttcggaaa atcggactcg aatgtcgatc tccttatctt tatcgtaaaa tttagtggga atagagcccg tatatgcggt tttcataaag tttgcgattt gattaggtaa taatccggat tgtgaggcct tcactttatc gatcgaaaaa gtgatttgat cttttccttc cttgaagcga agggcaacct gctgaattcc gggtatccct cctacttctt ttgcagattc tctggctaac tctttgatcc tttcggcttc gtctcctata atttcaatat cgatttcctt tgatccttct ccggcaccgg attcggtata aaatacaaat gcgttcttta acttgtctgt ttcctttttt aattcggtta taacgtcatt ggctgatttg ccttcccgag aagaggcggg ttttaacttt atcatcagat ccgaatgcca tttttcaact ttggaggata ctttttccac gtttggatta ttttcgatta ctctttccac gctcttggtg atcaaatcag tcgcatcaag gtgggttccc gtttctactt ccaacgaagc gctgatttcg ttggaatcgg tcggatcaat gtattccata tggtggtggt ggtggtgagc catgg

Expected Protein Sequence

MAHHHHHHEY IDPTDSNEIS ASLEVETGTH LDATDLITKS VERVIENNPN VEKVSSKVEK WHSDLMIKLK PASSREGKSA NDVITELKKE TDKLKNAFVF YTESGAGEGS KEIDIEIIGD EAERIKELAR ESAKEVGGIP GIQQVALRFK EGKDQITFSI DKVKASQSGL LPNQIANFMK TAYTGSIPTK FYDKDKEIDI RVRFSEEDRK GPESVFGVAI SSSQSEKIAK TPLIELSSIK KEKAESRIYR KNKRRTYGLT AKLGDIDTGS AVIKIETALK KISFPENYHY EMTGNYKKLK ESKTE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaataca ttgatccgac cgattccaac gaaatcagcg cttcgttgga agtagaaacg ggaacccacc ttgatgcgac tgatttgatc accaagagcg tggaaagagt aatcgaaaat aatccaaacg tggaaaaagt atcctccaaa gttgaaaaat ggcattcgga tctgatgata aagttaaaac ccgcctcttc tcgggaaggc aaatcagcca atgacgttat aaccgaatta aaaaaggaaa cagacaagtt aaagaacgca tttgtatttt ataccgaatc cggtgccgga gaaggatcaa aggaaatcga tattgaaatt ataggagacg aagccgaaag gatcaaagag ttagccagag aatctgcaaa agaagtagga gggatacccg gaattcagca ggttgccctt cgcttcaagg aaggaaaaga tcaaatcact ttttcgatcg ataaagtgaa ggcctcacaa tccggattat tacctaatca aatcgcaaac tttatgaaaa ccgcatatac gggctctatt cccactaaat tttacgataa agataaggag atcgacattc gagtccgatt ttccgaagaa gacagaaaag gtccggagtc ggtttttgga gttgcaattt cctcctctca atcggaaaaa atcgcaaaaa cacccttgat tgaacttagt tcgatcaaaa aggaaaaagc agaatccagg atttatagaa aaaacaaacg aagaacttat ggactcacgg ctaagttagg cgatatcgat accggttcag ccgttatcaa aattgaaacc gctttaaaga aaatttcttt cccggaaaat tatcattacg aaatgaccgg aaattacaaa aaactgaaag aaagtaaaac cgaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LpboA.18694.a.B2.PS38497

PURIFICATION DATe:	8/30/2018
CONCENTRATION:	24.42mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATTTCGT TAATGCCTCC ATCCAATTCG CCGGCTATTA CCATACTGAC TCGATATCCC GGAATTTCAC CCAGTAAAAT CGAAGAAATC ATAACGAAAC CGATAGAAGA ACAAATTTCC GGCGTAGGCG GAATCGAAGA TATACTATCT TCTTCCGAAG AAGGGGAATC AAGAATCAAC GTCATATTCA AACCCGGCAC TAAAATCTCG TATCGTTCCC TCGAAGTACG TTCTAAAATC GATCTCATAC GGGGTAACTT TCCAAGAGAA GTGGAAGAAC CCGCCATTCT TAGATACGAT CCTACCAACA AACCGATTTT CATCGTAAAA CTCGAATCCG AAAGATACAA ATTGAAGGAA CTGAGAGAAT TCGGCGAAAA CAAATATAAA AAGAAGTTCG AAAGAATCGA TGGAGTAAGT GAAATTCACG TCGGAGGTGG ATTTCAAAGA GAGATAAACA TAGATATAGA CAAAGGTAAG TTACTGCAGA CCGGAGTCGG GTTAGAAGAA GTCTTATCCA AGATCAGAAC TGCAAACGTA GACGTTCCCG CCGGAAATTT ATTGATCGGA GACAACTATA TCAACATTCG GGTGATCGGT AAGTTGATTC AAATTAAAGA GATCGGAAAA ATACCGATTA CGGTCGGCAA ATCGGAACAA ATTGTTTACT TATCGGACAT ATCGAATGTG AAGGACGAAT TCAAAGAAAA GGAAGATATA GCAAGAGACG CAGGTAAAGA AGTCGTATCC ATTTACGTTC AAAAAGCCGG TGATGCAAAT TCACTAGCGT TATGTGAAGA GCTCCGAAAG GAACTTTCCT CGATTCATAT AGAAGGAATT CAGCAAAGTA TCAACTATGA TCAATCTCAG ACAATCAAAA CGGCCATCGC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT