SSGCID: Target Page

LpboA.18182.a

Imidazoleglycerol-phosphate synthase

CENTER ID:	LpboA.18182.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.18182.a.B1.GE41858	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.1553
UniProt:	Q04TK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRPRIIPVL LLQENGLVKT IQFGDERYIG DPLNAVRIFN EKEADELAVL DISASKKGKE PNYRLIERLA NECRMPLCYG GGIKDLDQAN RILSFGVEKI IVSSLAIENP KMISTMASYL GSQSVVVAID FKKAMLSRRY EVMIHNGTKK TGKHLEDLVK EVIELGAGEI ILNSIDRDGT MVGYEIEIIK KIQSICKIPI TVLGGAGSLD HIKNLIQELG IIGVAAGSLF VYKGVHKAVL INYPNGTDKE ALFP

NT Sequence

atgttaagac ccagaatcat acctgtgttg ttgttacaag aaaacggatt ggtcaaaacg atccaattcg gcgacgaaag atatatcggt gacccattga atgccgtaag gatttttaat gaaaaagaag cggatgaact tgcggtttta gatatttccg ccagcaaaaa aggaaaggaa ccgaattatc gtctaatcga acgtttggcg aatgaatgca gaatgccttt gtgttacggc ggtgggatta aggatttgga tcaggcgaat cgaattttga gttttggtgt ggaaaaaata attgtaagtt cactcgcaat cgaaaatccg aaaatgattt ccactatggc ctcctacttg ggaagtcaga gtgtggtcgt agcaattgat tttaaaaaag cgatgttatc gagaagatat gaagtgatga ttcataacgg gactaaaaaa actggaaaac atctcgagga tttagtgaaa gaagttatcg aattgggcgc gggtgaaatc atactcaatt ccattgatcg cgacggaact atggtcggtt atgagatcga gatcatcaaa aagattcaaa gcatctgtaa gattcccatt acggttttag gtggagcggg gtcgctggat cacatcaaaa atctaattca ggaacttggc attatcggcg tagctgcggg aagtcttttt gtttataaag gagtacataa ggcggtttta atcaattatc cgaacggcac ggataaagaa gcactatttc cctga

Details for LpboA.18182.a.B1.GE41858

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHML RPRIIPVLLL QENGLVKTIQ FGDERYIGDP LNAVRIFNEK EADELAVLDI SASKKGKEPN YRLIERLANE CRMPLCYGGG IKDLDQANRI LSFGVEKIIV SSLAIENPKM ISTMASYLGS QSVVVAIDFK KAMLSRRYEV MIHNGTKKTG KHLEDLVKEV IELGAGEIIL NSIDRDGTMV GYEIEIIKKI QSICKIPITV LGGAGSLDHI KNLIQELGII GVAAGSLFVY KGVHKAVLIN YPNGXXKEAL FPXVS

Validated NT Sequence

tactcacnnn nggaaatagt gcttctttat nnnngccgtt cggataattg attaaaaccg ccttatgtac tcctttataa acaaaaagac ttcccgcagc tacgccgata atgccaagtt cctgaattag atttttgatg tgatccagcg accccgctcc acctaaaacc gtaatgggaa tcttacagat gctttgaatc tttttgatga tctcgatctc ataaccgacc atagttccgt cgcgatcaat ggaattgagt atgatttcac ccgcgcccaa ttcgataact tctttcacta aatcctcgag atgttttcca gtttttttag tcccgttatg aatcatcact tcatatcttc tcgataacat cgctttttta aaatcaattg ctacgaccac actctgactt cccaagtagg aggccatagt ggaaatcatt ttcggatttt cgattgcgag tgaacttaca attatttttt ccacaccaaa actcaaaatt cgattcgcct gatccaaatc cttaatccca ccgccgtaac acaaaggcat tctgcattca ttcgccaaac gttcgattag acgataattc ggttcctttc cttttttgct ggcggaaata tctaaaaccg caagttcatc cgcttctttt tcattaaaaa tccttacggc attcaatggg tcaccgatat atctttcgtc gccgaattgg atcgttttga ccaatccgtt ttcttgtaac aacaacacag gtatgattct gggtcttaac atatggtggt ggtggtggtg agccatggtn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa gacccagaat catacctgtg ttgttgttac aagaaaacgg attggtcaaa acgatccaat tcggcgacga aagatatatc ggtgacccat tgaatgccgt aaggattttt aatgaaaaag aagcggatga acttgcggtt ttagatattt ccgccagcaa aaaaggaaag gaaccgaatt atcgtctaat cgaacgtttg gcgaatgaat gcagaatgcc tttgtgttac ggcggtggga ttaaggattt ggatcaggcg aatcgaattt tgagttttgg tgtggaaaaa ataattgtaa gttcactcgc aatcgaaaat ccgaaaatga tttccactat ggcctcctac ttgggaagtc agagtgtggt cgtagcaatt gattttaaaa aagcgatgtt atcgagaaga tatgaagtga tgattcataa cgggactaaa aaaactggaa aacatctcga ggatttagtg aaagaagtta tcgaattggg cgcgggtgaa atcatactca attccattga tcgcgacgga actatggtcg gttatgagat cgagatcatc aaaaagattc aaagcatctg taagattccc attacggttt taggtggagc ggggtcgctg gatcacatca aaaatctaat tcaggaactt ggcattatcg gcgtagctgc gggaagtctt tttgtttata aaggagtaca taaggcggtt ttaatcaatt atccgaacgg cacggataaa gaagcactat ttccctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat