SSGCID: Target Page

LpboA.18136.b

Tetratricopeptide repeat family lipoprotein

CENTER ID:	LpboA.18136.b
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.18136.b.B2.GE41857	mature protein		27	187

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.4049
UniProt:	Q04NP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVPGLKSRFI SIFIFFLTIT LFSACSFKQE KTKELVFIRK TDLNSFQEGN SKFKNGDYLE AIEFYSRDLD VNPDNPASLN NRGLAKSKSG NKTGAISDYD QAIEKRENYS IAFNNRGCAK MKVSDYRGVI EDLSLAIRLK PTYANALNNR AAVNWTVKEQ QSACEDWKKA ANLGHNEAEK SFVKFCN

NT Sequence

ttggtccccg gtcttaaatc gagatttata tcgattttta tttttttcct aactattacg ctgttttctg cctgttcttt caaacaggag aaaacaaagg aactggtttt catccgcaaa accgatctga attcctttca agaggggaat tctaaattca agaacgggga ctatttggaa gcgatcgagt tttattcaag agatctggac gtcaatccgg acaatccggc ctcacttaac aatagaggac ttgccaaaag caaatccggt aacaaaacag gagcgatttc cgattacgac caggcgattg aaaaacgaga gaattactcg atcgctttca acaaccgagg ctgtgcaaaa atgaaagttt ccgattatcg aggggtgatc gaagatcttt ctttagcgat ccgtttgaaa ccaacttatg cgaacgcact caataaccgc gctgcggtaa actggacagt gaaagaacaa caaagcgctt gcgaagattg gaaaaaagcg gcaaacttag ggcataacga agccgaaaaa tcctttgtaa aattctgcaa ttga

Details for LpboA.18136.b.B2.GE41857

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/29/2017
EXPECTED MW:	19kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF KQEKTKELVF IRKTDLNSFQ EGNSKFKNGD YLEAIEFYSR DLDVNPDNPA SLNNRGLAKS KSGNKTGAIS DYDQAIEKRE NYSIAFNNRG CAKMKVSDYR GVIEDLSLAI RLKPTYANAL NNRAAVNWTV KEQQSACEDW KKAANLGHNE AEKSFVKFCN

Validated NT Sequence

atcttactca cttaattgca gaattttaca aaggattttt cggcttcgtt atgccctaag tttgccgctt ttttccaatc ttcgcaagcg ctttgttgtt ctttcactgt ccagtttacc gcagcgcggt tattgagtgc gttcgcataa gttggtttca aacggatcgc taaagaaaga tcttcgatca cccctcgata atcggaaact ttcatttttg cacagcctcg gttgttgaaa gcgatcgagt aattctctcg tttttcaatc gcctggtcgt aatcggaaat cgctcctgtt ttgttaccgg atttgctttt ggcaagtcct ctattgttaa gtgaggccgg attgtccgga ttgacgtcca gatctcttga ataaaactcg atcgcttcca aatagtcccc gttcttgaat ttagaattcc cctcttgaaa ggaattcaga tcggttttgc ggatgaaaac cagttccttt gttttctcct gtttgaacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHFK QEKTKELVFI RKTDLNSFQE GNSKFKNGDY LEAIEFYSRD LDVNPDNPAS LNNRGLAKSK SGNKTGAISD YDQAIEKREN YSIAFNNRGC AKMKVSDYRG VIEDLSLAIR LKPTYANALN NRAAVNWTVK EQQSACEDWK KAANLGHNEA EKSFVKFCN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcaaac aggagaaaac aaaggaactg gttttcatcc gcaaaaccga tctgaattcc tttcaagagg ggaattctaa attcaagaac ggggactatt tggaagcgat cgagttttat tcaagagatc tggacgtcaa tccggacaat ccggcctcac ttaacaatag aggacttgcc aaaagcaaat ccggtaacaa aacaggagcg atttccgatt acgaccaggc gattgaaaaa cgagagaatt actcgatcgc tttcaacaac cgaggctgtg caaaaatgaa agtttccgat tatcgagggg tgatcgaaga tctttcttta gcgatccgtt tgaaaccaac ttatgcgaac gcactcaata accgcgctgc ggtaaactgg acagtgaaag aacaacaaag cgcttgcgaa gattggaaaa aagcggcaaa cttagggcat aacgaagccg aaaaatcctt tgtaaaattc tgcaattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t