SSGCID: Target Page

LpboA.18136.a

Uncharacterized protein

CENTER ID:	LpboA.18136.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.18136.a.B1.GE41856	full length		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.3504
UniProt:	Q04PN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVRCFRGQF GTDQFHEMME AGLSEYLNLP GNMDYFLLRL CEEIKNEMWH ETLDVSELVS QIPNIFEDLD LRYPAKKIRI LAKEIASAEK THQARSQSVR DRVERGWEHV RKEEYTQAEE SVRSALSEYP EDAQALYLDT RLYRLISGSV EAGLQRAQNN LKRAAKFDSF GIAALYNLIG CSFEELSRNE EAIQAIEKAT KIVPNESMYI ANLAEIHWKI GNKQAAIEYA QKAKRLGKKS ELIDAILKES VNANLHKNN

NT Sequence

atggctgtgc gttgttttcg aggtcaattc ggaaccgatc aattccatga aatgatggaa gcgggacttt ccgaatattt gaatttgccc ggcaatatgg attatttttt gcttagattg tgtgaagaaa taaaaaacga aatgtggcat gagacactgg atgtttccga actcgtatca caaatcccta atatatttga agatttggat ctgcgctatc ccgcaaaaaa aatccgaata ctggcgaaag agatagcttc ggcagaaaaa actcatcaag ctcgttctca aagcgtgcgc gatcgagtag aacggggttg ggagcatgtt cgaaaagaag aatatactca agcggaagaa tcggttcgat ccgctctttc ggaatatccg gaagacgcac aggccttgta tttggataca aggctttacc gactgatttc cggctcggta gaagcgggtc tccaacgcgc ccaaaataat ttaaaaagag ccgcaaagtt tgattctttc ggaatcgctg cgctttacaa tttgatcgga tgtagtttcg aagagctttc tcgcaatgaa gaagcgatcc aagccattga aaaagcgacc aaaattgtcc cgaacgaatc gatgtatata gccaatcttg cagagataca ttggaaaatc ggaaataaac aagctgcaat cgaatacgct caaaaggcga aaagattggg aaaaaagtcc gagttgattg atgcgatact caaagagagt gtaaatgcga atctacacaa aaacaattag

Details for LpboA.18136.a.B1.GE41856

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA VRCFRGQFGT DQFHEMMEAG LSEYLNLPGN MDYFLLRLCE EIKNEMWHET LDVSELVSQI PNIFEDLDLR YPAKKIRILA KEIASAEKTH QARSQSVRDR VERGWEHVRK EEYTQAEESV RSALSEYPED AQALYLDTRL YRLISGSVEA GLQRAQNNLK RAAKFDSFGI AALYNLIGCS FEELSRNEEA IQAIEKATKI VPNESMYIAN LAEIHWKIGN KQAAIEYAQK AKRLGKKSEL IDAILKESVN GNLHKNN

Validated NT Sequence

atcttactca cttaattgtt tttgtgtaga ttcccattta cactctcttt gagtatcgca tcaatcaact cggacttttt tcccaatctt ttcgcctttt gagcgtattc gattgcagct tgtttatttc cgattttcca atgtatctct gcaagattgg ctatatacat cgattcgttc gggacaattt tggtcgcttt ttcaatggct tggatcgctt cttcattgcg agaaagctct tcgaaactac atccgatcaa attgtaaagc gcagcgattc cgaaagaatc aaactttgcg gctcttttta aattattttg ggcgcgttgg agacccgctt ctaccgagcc ggaaatcagt cggtaaagcc ttgtatccaa atacaaggcc tgtgcgtctt ccggatattc cgaaagagcg gatcgaaccg attcttccgc ttgagtatat tcttcttttc gaacatgctc ccaaccccgt tctactcgat cgcgcacgct ttgagaacga gcttgatgag ttttttctgc cgaagctatc tctttcgcca gtattcggat tttttttgcg ggatagcgca gatccaaatc ttcaaatata ttagggattt gtgatacgag ttcggaaaca tccagtgtct catgccacat ttcgtttttt atttcttcac acaatctaag caaaaaataa tccatattgc cgggcaaatt caaatattcg gaaagtcccg cttccatcat ttcatggaat tgatcggttc cgaattgacc tcgaaaacaa cgcacagcca tatggtggtg gtggtggtga gccatggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg tgcgttgttt tcgaggtcaa ttcggaaccg atcaattcca tgaaatgatg gaagcgggac tttccgaata tttgaatttg cccggcaata tggattattt tttgcttaga ttgtgtgaag aaataaaaaa cgaaatgtgg catgagacac tggatgtttc cgaactcgta tcacaaatcc ctaatatatt tgaagatttg gatctgcgct atcccgcaaa aaaaatccga atactggcga aagagatagc ttcggcagaa aaaactcatc aagctcgttc tcaaagcgtg cgcgatcgag tagaacgggg ttgggagcat gttcgaaaag aagaatatac tcaagcggaa gaatcggttc gatccgctct ttcggaatat ccggaagacg cacaggcctt gtatttggat acaaggcttt accgactgat ttccggctcg gtagaagcgg gtctccaacg cgcccaaaat aatttaaaaa gagccgcaaa gtttgattct ttcggaatcg ctgcgcttta caatttgatc ggatgtagtt tcgaagagct ttctcgcaat gaagaagcga tccaagccat tgaaaaagcg accaaaattg tcccgaacga atcgatgtat atagccaatc ttgcagagat acattggaaa atcggaaata aacaagctgc aatcgaatac gctcaaaagg cgaaaagatt gggaaaaaag tccgagttga ttgatgcgat actcaaagag agtgtaaatg cgaatctaca caaaaacaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat