LpboA.17669.a

Sugar oxidoreductase

CENTER ID: LpboA.17669.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.17669.a.B2.GE41854 mature protein 26 298
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1558
UniProt: Q04TJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSPKKILVL GVSGMLGNAV FRVLSENAEF EVQGTIRSIE YLRFFTKAES ERITSNMDVL NEDELLRLFS NIKPDIVVNC VGIIKQQKLS KDPITVLPIN SLLPHRLSNL CKLIGARLIL ISTDCVFNGQ KGNYTETDTP DAEDLYGRSK EIGEIREESH VFTVRTSIIG HELNSNYSLV DWFLSQNGEV KGYKKAFFSG LPSCEIAEII KTVIIPNPKL YGLYHVSSDP ISKFDLLSLV GEVYGKNNKI LESEEVIVDR SLDSTKFRKE TSYKPLPWKS LIFLMKKYKE QYLDNIHV
NT Sequence
gtgttatccc caaaaaaaat attagtttta ggcgtatccg gaatgttggg aaacgcagtg tttcgcgttc tatccgaaaa cgccgaattt gaagttcaag gaacgattag aagtattgaa tatttgagat tctttacgaa agcggaatcg gaaaggatca catcgaatat ggacgtttta aacgaggacg agttgcttcg tttattttca aacataaaac cggatatcgt tgttaattgt gtgggaatca tcaaacagca aaaattgtca aaggatccaa ttactgtttt gcccatcaat tctcttttac cgcacagact ttccaatcta tgcaaattaa tcggggccag attgattttg atcagcacgg attgtgtttt taacggtcaa aaagggaatt atacggaaac tgatacgccc gatgcggaag atttatacgg cagatccaaa gaaatcggag aaatccggga agaatctcat gtgtttacgg tacgcacttc cattattggc catgaactca attctaacta ttccttggtt gattggtttt tatcacaaaa cggtgaagtg aaaggataca aaaaggcctt tttttcgggc cttccttcct gcgaaatcgc cgaaatcatt aagacagtaa tcattcccaa tccgaaactt tatggactct atcatgtttc ttctgatcct atttctaaat tcgatttgct gagtttagtc ggagaagtgt atggaaaaaa taataaaatt ttagaaagcg aagaagtaat tgtagatcgc tctttggatt caaccaaatt cagaaaagaa acttcttata aacctttgcc atggaagagt ttaatttttt tgatgaaaaa gtacaaagaa caatatctgg ataatatcca tgtttaa
Details for LpboA.17669.a.B2.GE41854
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME NAEFEVQGTI RSIEYLRFFT KAESERITSN MDVLNEDELL RLFSNIKPDI VVNCVGIIKQ QKLSKDPITV LPINSLLPHR LSNLCKLIGA RLILISTDCV FNGQKGNYTE TDTPDAEDLY GRSKEIGEIR EESHVFTVRT SIIGHELNSN YSLVDWFLSQ NGEVKGYKKA FFSGLPSCEI AEIIKTVIIP NPKLYGLYHV SSDPISKFDL LSLVGEVYGK NNKILESEEV IVDRSLDSTK FRKETSYKPL PWKSLIFLMK KYKEQYLDNI HV
Validated NT Sequence
atcttactca cttaaacatg gatattatcc agatattgtt ctttgtactt tttcatcaaa aaaattaaac tcttccatgg caaaggttta taagaagttt cttttctgaa tttggttgaa tccaaagagc gatctacaat tacttcttcg ctttctaaaa ttttattatt ttttccatac acttctccga ctaaactcag caaatcgaat ttagaaatag gatcagaaga aacatgatag agtccataaa gtttcggatt gggaatgatt actgtcttaa tgatttcggc gatttcgcag gaaggaaggc ccgaaaaaaa ggcctttttg tatcctttca cttcaccgtt ttgtgataaa aaccaatcaa ccaaggaata gttagaattg agttcatggc caataatgga agtgcgtacc gtaaacacat gagattcttc ccggatttct ccgatttctt tggatctgcc gtataaatct tccgcatcgg gcgtatcagt ttccgtataa ttcccttttt gaccgttaaa aacacaatcc gtgctgatca aaatcaatct ggccccgatt aatttgcata gattggaaag tctgtgcggt aaaagagaat tgatgggcaa aacagtaatt ggatcctttg acaatttttg ctgtttgatg attcccacac aattaacaac gatatccggt tttatgtttg aaaataaacg aagcaactcg tcctcgttta aaacgtccat attcgatgtg atcctttccg attccgcttt cgtaaagaat ctcaaatatt caatacttct aatcgttcct tgaacttcaa attcggcgtt ttccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHEN AEFEVQGTIR SIEYLRFFTK AESERITSNM DVLNEDELLR LFSNIKPDIV VNCVGIIKQQ KLSKDPITVL PINSLLPHRL SNLCKLIGAR LILISTDCVF NGQKGNYTET DTPDAEDLYG RSKEIGEIRE ESHVFTVRTS IIGHELNSNY SLVDWFLSQN GEVKGYKKAF FSGLPSCEIA EIIKTVIIPN PKLYGLYHVS SDPISKFDLL SLVGEVYGKN NKILESEEVI VDRSLDSTKF RKETSYKPLP WKSLIFLMKK YKEQYLDNIH V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaaacg ccgaatttga agttcaagga acgattagaa gtattgaata tttgagattc tttacgaaag cggaatcgga aaggatcaca tcgaatatgg acgttttaaa cgaggacgag ttgcttcgtt tattttcaaa cataaaaccg gatatcgttg ttaattgtgt gggaatcatc aaacagcaaa aattgtcaaa ggatccaatt actgttttgc ccatcaattc tcttttaccg cacagacttt ccaatctatg caaattaatc ggggccagat tgattttgat cagcacggat tgtgttttta acggtcaaaa agggaattat acggaaactg atacgcccga tgcggaagat ttatacggca gatccaaaga aatcggagaa atccgggaag aatctcatgt gtttacggta cgcacttcca ttattggcca tgaactcaat tctaactatt ccttggttga ttggttttta tcacaaaacg gtgaagtgaa aggatacaaa aaggcctttt tttcgggcct tccttcctgc gaaatcgccg aaatcattaa gacagtaatc attcccaatc cgaaacttta tggactctat catgtttctt ctgatcctat ttctaaattc gatttgctga gtttagtcgg agaagtgtat ggaaaaaata ataaaatttt agaaagcgaa gaagtaattg tagatcgctc tttggattca accaaattca gaaaagaaac ttcttataaa cctttgccat ggaagagttt aatttttttg atgaaaaagt acaaagaaca atatctggat aatatccatg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat