LpboA.17492.a

Carbamoyl transferase

CENTER ID: LpboA.17492.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.17492.a.B1.GE41853 full length 1 583
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1513
UniProt: Q04TN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKILGINAFG HDTSAALVID GKLIAAVEEE RLNREKSTRT FPHLSIEYCL KEANIKFEDV DYITYAYQPY LWLTHRFLYH QLRFFPQALG ELKYVKRMIS KFMFLKSNIQ KELKTNKPII LLKHHDAHMG STFLVSPFEE AAILTLDGLG EYETSVQAVG KGTSIKRLDH ILFPNSLGSM YACISHFVGY TAELDEGKVM GLAAYGDASE YYNDFKKLVK LKKDGTFQLD LSYFEYHKKR DTWVSDKFYK VFGPRRAKNE EMTSRYINVA AAAQKVLEDT VLHISESLYN RTKMKAICLA GGVALNSVAN GRIVEQGLFK DIFIQPASGD NGLPIGAAFY VYNTLKKQKR VFVERDTYLG PKYPEQDIIS ALKKYNLPVY KSDSVAKETA AMLTNKNVIG LYQGRMEFGP RALGNRSIIA DPRFEDMKDI VNEKVKFREA YRPFAPAILA DHCKDYFEND YTSPYMLLVY KARPFAKDKI PAVVHQDGTG RVQTVEKDIN PRYYNIIEEF YKITNVPVIM NTSFNIKDEP IVCNPEDAVR CFLGTNIDAL VLEDYIVKKT DIDMSKYGNQ EDYRHNRGQA SKH
NT Sequence
atgaagatat taggtattaa tgcattcgga catgatacgt cagctgctct cgttatcgat gggaaattga ttgcggcagt agaagaggaa cggttgaatc gagaaaaaag tactcgcacc ttccctcatc tatcgataga gtattgctta aaagaagcga atattaaatt tgaagatgtc gactatatta cttatgcgta tcagccatat ctttggctaa ctcaccgatt tttgtatcat caattgcgct ttttccctca ggctttagga gagcttaagt atgtgaagcg tatgatttca aagtttatgt ttttgaaatc taatattcaa aaagaactaa aaacgaataa gccaattata ttattgaagc atcatgatgc gcatatggga agtacatttc ttgtttctcc gtttgaagag gcagctattt taacattgga tggattgggt gaatatgaga ctagtgtaca agctgtcggt aaaggaacgt cgatcaaaag attagatcat attctttttc caaattcact tgggtcgatg tatgcctgta tctcgcattt tgttggatat acagcggaat tggacgaggg aaaagttatg gggcttgcgg catatggaga tgcttcggag tactataatg attttaagaa gctcgttaaa ctaaagaaag atggaacctt tcaattagat ttatcttatt ttgaatatca taaaaaaaga gatacctggg taagcgataa gttttataaa gtttttggcc ctcggcgtgc caaaaatgag gaaatgacat cacgttatat taatgtagca gcggcagctc aaaaagtttt ggaagacacg gtattgcata tttctgaatc tttatataat agaaccaaaa tgaaggcgat ctgtctggct ggtggagtcg ctctgaatag cgttgcaaat gggaggatcg ttgaacaagg tttgtttaag gatattttta ttcaacctgc gagcggggat aacggtcttc ctatcggtgc ggctttttac gtatataata ctctgaagaa acaaaaaaga gtgtttgtgg aaagagatac ctatttgggt ccaaagtatc cagagcaaga tattatcagt gctttaaaga aatataattt acctgtttat aaatcagatt ctgttgctaa agaaactgcg gcgatgttga caaataaaaa tgtgattggg ttgtatcaag gacgaatgga atttggccct agggctttag gaaatcgaag tattattgcg gatccccgat ttgaagatat gaaggacatt gtaaatgaaa aagtaaagtt tagagaagct tatcgcccgt ttgcaccggc tattttagca gatcattgca aggattattt tgaaaatgat tatacttctc cctatatgct acttgtttac aaagcaaggc catttgctaa agataaaatt cccgcagttg tccatcaaga cggaacggga agagtacaga cggtagaaaa ggacattaat ccaaggtatt ataatattat tgaagagttt tataaaatta ctaatgttcc ggtaattatg aatacatcgt tcaatatcaa agacgagccg atcgtgtgta accctgaaga tgctgtacgt tgttttttgg gaaccaatat tgatgcgtta gtattggaag attatattgt aaaaaagact gatatcgata tgagtaaata tggtaaccaa gaagattatc gccacaatag aggccaggct agcaagcact ag
Details for LpboA.17492.a.B1.GE41853
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK ILGINAFGHD TSAALVIDGK LIAAVEEERL NREKSTRTFP HLSIEYCLKE ANIKFEDVDY ITYAYQPYLW LTHRFLYHQL RFFPQALGEL KYVKRMISKF MFLKSNIQKE LKTNKPIILL KHHDAHMGST FLVSPFEEAA ILTLDGLGEY ETSVQAVGKG TSIKRLDHIL FPNSLGSMYA CISHFVGYTA ELDEGKVMGL AAYGDASEYY NDFKKLVKLK KDGTFQLDLS YFEYHKKRDT WVSDKFYKVF GPRRAKNEEM TSRYINVAAA AQKVLEXTVL HISXXLYNRT KMKAICLAGG VALNSVANGR IVEQGLFKDI FIQPASGDNG LPIGAAFYVY NTLKKQKRVF VERDTYLGPK YPEQDIISAL KKYNLPVYKS DSVAKETAAM LTNKNVIGLY QGRMEFGPRA LGNRSIIADP RFEDMKDIVN EKVKFREAYR PFAPAILADH CKDYFENDYT SPYMLLVYKA RPFAKDKIPA VVHQDGTGRV QTVEKDINPR YYNIIEEFYK ITNVPVIMNT SFNIKDEPIV CNPEDAVRCF LGTNIDALVL EDYIVKKTDI DMSKYGNQED YRHNRGQASK H
Validated NT Sequence
atggctcacc accaccacca ccatatgaag atattaggta ttaatgcatt cggacatgat acgtcagctg ctctcgttat cgatgggaaa ttgattgcgg cagtagaaga ggaacggttg aatcgagaaa aaagtactcg caccttccct catctatcga tagagtattg cttaaaagaa gcgaatatta aatttgaaga tgtcgactat attacttatg cgtatcagcc atatctttgg ctaactcacc gatttttgta tcatcaattg cgctttttcc ctcaggcttt aggagagctt aagtatgtga agcgtatgat ttcaaagttt atgtttttga aatctaatat tcaaaaagaa ctaaaaacga ataagccaat tatattattg aagcatcatg atgcgcatat gggaagtaca tttcttgttt ctccgtttga agaggcagct attttaacat tggatggatt gggtgaatat gagactagtg tacaagctgt cggtaaagga acgtcgatca aaagattaga tcatattctt tttccaaatt cacttgggtc gatgtatgcc tgtatctcgc attttgttgg atatacagcg gaattggacg agggaaaagt tatggggctt gcggcatatg gagatgcttc ggagtactat aatgatttta agaagctcgt taaactaaag aaagatggaa cctttcaatt agatttatct tattttgaat atcataaaaa aagagatacc tgggtaagcg ataagtttta taaagttttt ggccctcggc gtgccaaaaa tgaggaaatg acatcacgtt atattaatgt agcagcggca gctcaaaaag ttttggaaga nacggtattg catatttctg annctttata taatagaacc aaaatgaagg cgatctgtct ggctggtgga gtcgctctga atagcgttgc aaatgggagg atcgttgaac aa-ggtttgt ttaaggatat ttttattcaa cctgcgagcg gggataacgg tcttcctatc ggtgcggctt tttacgtata taatactctg aagaaacaaa aaagagtgtt tgtggaaaga gatacctatt tgggtccaaa gtatccagag caagatatta tcagtgcttt aaagaaatat aatttacctg tttataaatc agattctgtt gctaaagaaa ctgcggcgat gttgacaaat aaaaatgtga ttgggttgta tcaaggacga atggaatttg gccctagggc tttaggaaat cgaagtatta ttgcggatcc ccgatttgaa gatatgaagg acattgtaaa tgaaaaagta aagtttagag aagcttatcg cccgtttgca ccggctattt tagcagatca ttgcaaggat tattttgaaa atgattatac ttctccctat atgctacttg tttacaaagc aaggccattt gctaaagata aaattcccgc agttgtccat caagacggaa cgggaagagt acagacggta gaaaaggaca ttaatccaag gtattataat attattgaag agttttataa aattactaat gttccggtaa ttatgaatac atcgttcaat atcaaagacg agccgatcgt gtgtaaccct gaagatgctg tacgttgttt tttgggaacc aatattgatg cgttagtatt ggaagattat attgtaaaaa agactgatat cgatatgagt aaatatggta accaagaaga ttatcgccac aatagaggcc aggctagcaa gcactaa
Expected Protein Sequence
MAHHHHHHMK ILGINAFGHD TSAALVIDGK LIAAVEEERL NREKSTRTFP HLSIEYCLKE ANIKFEDVDY ITYAYQPYLW LTHRFLYHQL RFFPQALGEL KYVKRMISKF MFLKSNIQKE LKTNKPIILL KHHDAHMGST FLVSPFEEAA ILTLDGLGEY ETSVQAVGKG TSIKRLDHIL FPNSLGSMYA CISHFVGYTA ELDEGKVMGL AAYGDASEYY NDFKKLVKLK KDGTFQLDLS YFEYHKKRDT WVSDKFYKVF GPRRAKNEEM TSRYINVAAA AQKVLEDTVL HISESLYNRT KMKAICLAGG VALNSVANGR IVEQGLFKDI FIQPASGDNG LPIGAAFYVY NTLKKQKRVF VERDTYLGPK YPEQDIISAL KKYNLPVYKS DSVAKETAAM LTNKNVIGLY QGRMEFGPRA LGNRSIIADP RFEDMKDIVN EKVKFREAYR PFAPAILADH CKDYFENDYT SPYMLLVYKA RPFAKDKIPA VVHQDGTGRV QTVEKDINPR YYNIIEEFYK ITNVPVIMNT SFNIKDEPIV CNPEDAVRCF LGTNIDALVL EDYIVKKTDI DMSKYGNQED YRHNRGQASK H
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tattaggtat taatgcattc ggacatgata cgtcagctgc tctcgttatc gatgggaaat tgattgcggc agtagaagag gaacggttga atcgagaaaa aagtactcgc accttccctc atctatcgat agagtattgc ttaaaagaag cgaatattaa atttgaagat gtcgactata ttacttatgc gtatcagcca tatctttggc taactcaccg atttttgtat catcaattgc gctttttccc tcaggcttta ggagagctta agtatgtgaa gcgtatgatt tcaaagttta tgtttttgaa atctaatatt caaaaagaac taaaaacgaa taagccaatt atattattga agcatcatga tgcgcatatg ggaagtacat ttcttgtttc tccgtttgaa gaggcagcta ttttaacatt ggatggattg ggtgaatatg agactagtgt acaagctgtc ggtaaaggaa cgtcgatcaa aagattagat catattcttt ttccaaattc acttgggtcg atgtatgcct gtatctcgca ttttgttgga tatacagcgg aattggacga gggaaaagtt atggggcttg cggcatatgg agatgcttcg gagtactata atgattttaa gaagctcgtt aaactaaaga aagatggaac ctttcaatta gatttatctt attttgaata tcataaaaaa agagatacct gggtaagcga taagttttat aaagtttttg gccctcggcg tgccaaaaat gaggaaatga catcacgtta tattaatgta gcagcggcag ctcaaaaagt tttggaagac acggtattgc atatttctga atctttatat aatagaacca aaatgaaggc gatctgtctg gctggtggag tcgctctgaa tagcgttgca aatgggagga tcgttgaaca aggtttgttt aaggatattt ttattcaacc tgcgagcggg gataacggtc ttcctatcgg tgcggctttt tacgtatata atactctgaa gaaacaaaaa agagtgtttg tggaaagaga tacctatttg ggtccaaagt atccagagca agatattatc agtgctttaa agaaatataa tttacctgtt tataaatcag attctgttgc taaagaaact gcggcgatgt tgacaaataa aaatgtgatt gggttgtatc aaggacgaat ggaatttggc cctagggctt taggaaatcg aagtattatt gcggatcccc gatttgaaga tatgaaggac attgtaaatg aaaaagtaaa gtttagagaa gcttatcgcc cgtttgcacc ggctatttta gcagatcatt gcaaggatta ttttgaaaat gattatactt ctccctatat gctacttgtt tacaaagcaa ggccatttgc taaagataaa attcccgcag ttgtccatca agacggaacg ggaagagtac agacggtaga aaaggacatt aatccaaggt attataatat tattgaagag ttttataaaa ttactaatgt tccggtaatt atgaatacat cgttcaatat caaagacgag ccgatcgtgt gtaaccctga agatgctgta cgttgttttt tgggaaccaa tattgatgcg ttagtattgg aagattatat tgtaaaaaag actgatatcg atatgagtaa atatggtaac caagaagatt atcgccacaa tagaggccag gctagcaagc actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat