SSGCID: Target Page

LpboA.17333.b

Pyridoxal phosphate-dependent aminotransferase

CENTER ID:	LpboA.17333.b
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.17333.b.B1.GE41852	full length		1	387

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.1512
UniProt:	Q04TP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPGFELIGNE EFGELEDLFQ RSKILFRHGF DGIRNGVFKV REFELAFAKH FGFQDALAVT SGTAALKVAM KAANIKPGDE VITQSFTFLA TVEAIVEAGA TPIICEIDES LNMDPIALEK LITQKTRMIV PVHMLGVPAR MGQILAIARK YNLLVMEDTA WGCGGKFQGS YLGTLGDIGS FSFDFAKIIT TGEGGMILAK DQSLLERAKA YHDHGHENNP KLPRWEDSRS NSGFNYRMTE MQGAVGLAQL KKLGEIVKLQ RANLEKIKSV VSRYEGIRFR DVPESGEETA DALVFFTKTP KLARAFRLAM LEEGLGTKIL PEAISWHFAG TWNHIPELVR QYPNLFEAFP RSLELLDRSV AIPIQVKMQD QVLDKVDSAM IKVFKGN

NT Sequence

atgcccggat ttgaactaat aggaaatgaa gagtttggag aattggaaga tttatttcaa aggagtaaaa tactttttcg gcatggattt gacgggattc ggaatggggt ttttaaggta agagaatttg aacttgcttt tgcgaaacat ttcgggtttc aggatgccct agcggttact tccggaactg cagctttgaa agtagcgatg aaagctgcta atattaaacc tggggatgag gtgatcaccc agtcttttac attcttagca actgtggaag cgattgtaga ggcaggtgct actccaatca tttgtgagat agatgaatct ctcaatatgg atccaattgc tcttgaaaaa ttgatcactc aaaagactag gatgattgtt ccagtgcata tgttaggtgt ccccgcaagg atgggtcaaa ttctcgcgat tgcccgaaag tataatcttt tagttatgga ggatactgct tggggatgtg gcggaaaatt tcagggctca tacctcggaa ctttaggaga tattggtagt tttagttttg attttgcaaa gatcataact actggcgaag gtggaatgat acttgcaaag gatcagagtt tattggaaag agctaaagca tatcatgatc atgggcacga aaataaccca aagttacctc gctgggaaga ctctagaagt aattcgggtt ttaactatcg aatgacagaa atgcagggag ccgttggtct tgctcaactt aaaaagttgg gtgaaatcgt gaagttgcaa agagctaatt tagagaaaat taaatcggtt gtatctcgat atgagggtat ccgttttaga gatgttcccg agtccggtga agaaactgct gacgctttgg tcttttttac aaaaactcca aaattggcgc gtgcttttcg gctagcgatg cttgaagaag gtcttggtac taaaatactc cctgaagcta tttcttggca ttttgctgga acttggaatc atattcccga acttgttcgt cagtatccta atttgttcga agcttttcca cgctctttag aattattaga tagaagcgta gccatcccaa ttcaagtaaa gatgcaagat caagtcttgg ataaagtgga ttctgccatg attaaggtgt ttaaaggtaa ttaa

Details for LpboA.17333.b.B1.GE41852

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP GFELIGNEEF GELEDLFQRS KILFRHGFDG IRNGVFKVRE FELAFAKHFG FQDALAVTSG TAALKVAMKA ANIKPGDEVI TQSFTFLATV EAIVEAGATP IICEIDESLN MDPIALEKLI TQKTRMIVPV HMLGVPARMG QILAIARKYN LLVMEDTAWG CGGKFQGSYL GTLGDIGSFS FDFAKIITTG EGGMILAKDQ SLLERAKAYH DHGHENNPKL PRWEDSRSNS GFNYRMTEMQ GAVGLAQLKK LGEIVKLQRA NLEKIKSVVS RYEGIRFRDV PESGEETADA LVFFTKTPKL ARAFRLAMLE EGLGTKILPE AISWHFAGTW NHIPELVRQY PNLFEAFPRS LELLDRSVAI PIQVKMQDQV LDKVDS

Validated NT Sequence

cagaatccac tttatccaag acttgatctt gcatctttac ttgaattggg atggctacgc ttctatctaa taattctaaa gagcgtggaa aagcttcgaa caaattagga tactgacgaa caagttcggg aatatgattc caagttccag caaaatgcca agaaatagct tcagggagta ttttagtacc aagaccttct tcaagcatcg ctagccgaaa agcacgcgcc aattttggag tttttgtaaa aaagaccaaa gcgtcagcag tttcttcacc ggactcggga acatctctaa aacggatacc ctcatatcga gatacaaccg atttaatttt ctctaaatta gctctttgca acttcacgat ttcacccaac tttttaagtt gagcaagacc aacggctccc tgcatttctg tcattcgata gttaaaaccc gaattacttc tagagtcttc ccagcgaggt aactttgggt tattttcgtg cccatgatca tgatatgctt tagctctttc caataaactc tgatcctttg caagtatcat tccaccttcg ccagtagtta tgatctttgc aaaatcaaaa ctaaaactac caatatctcc taaagttccg aggtatgagc cctgaaattt tccgccacat ccccaagcag tatcctccat aactaaaaga ttatactttc gggcaatcgc gagaatttga cccatccttg cggggacacc taacatatgc actggaacaa tcatcctagt cttttgagtg atcaattttt caagagcaat tggatccata ttgagagatt catctatctc acaaatgatt ggagtagcac ctgcctctac aatcgcttcc acagttgcta agaatgtaaa agactgggtg atcacctcat ccccaggttt aatattagca gctttcatcg ctactttcaa agctgcagtt ccggaagtaa ccgctagggc atcctgaaac ccgaaatgtt tcgcaaaagc aagttcaaat tctcttacct taaaaacccc attccgaatc ccgtcaaatc catgccgaaa aagtatttta ctcctttgaa ataaatcttc caattctcca aactcttcat ttcctattag ttcaaatccg ggcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg gatttgaact aataggaaat gaagagtttg gagaattgga agatttattt caaaggagta aaatactttt tcggcatgga tttgacggga ttcggaatgg ggtttttaag gtaagagaat ttgaacttgc ttttgcgaaa catttcgggt ttcaggatgc cctagcggtt acttccggaa ctgcagcttt gaaagtagcg atgaaagctg ctaatattaa acctggggat gaggtgatca cccagtcttt tacattctta gcaactgtgg aagcgattgt agaggcaggt gctactccaa tcatttgtga gatagatgaa tctctcaata tggatccaat tgctcttgaa aaattgatca ctcaaaagac taggatgatt gttccagtgc atatgttagg tgtccccgca aggatgggtc aaattctcgc gattgcccga aagtataatc ttttagttat ggaggatact gcttggggat gtggcggaaa atttcagggc tcatacctcg gaactttagg agatattggt agttttagtt ttgattttgc aaagatcata actactggcg aaggtggaat gatacttgca aaggatcaga gtttattgga aagagctaaa gcatatcatg atcatgggca cgaaaataac ccaaagttac ctcgctggga agactctaga agtaattcgg gttttaacta tcgaatgaca gaaatgcagg gagccgttgg tcttgctcaa cttaaaaagt tgggtgaaat cgtgaagttg caaagagcta atttagagaa aattaaatcg gttgtatctc gatatgaggg tatccgtttt agagatgttc ccgagtccgg tgaagaaact gctgacgctt tggtcttttt tacaaaaact ccaaaattgg cgcgtgcttt tcggctagcg atgcttgaag aaggtcttgg tactaaaata ctccctgaag ctatttcttg gcattttgct ggaacttgga atcatattcc cgaacttgtt cgtcagtatc ctaatttgtt cgaagctttt ccacgctctt tagaattatt agatagaagc gtagccatcc caattcaagt aaagatgcaa gatcaagtct tggataaagt ggattctgcc atgattaagg tgtttaaagg taattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat