SSGCID: Target Page

LpboA.17333.a

Pyridoxal-phosphate-dependent aminotransferase

CENTER ID:	LpboA.17333.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.17333.a.B1.GE41851	full length		1	401

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.1531
UniProt:	Q04TM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKLAIHGGE PVRKSFLVYG APDIGDEEIQ AVVNCIKTGW LSTGPNVQKF ETDIKNYTGA KHAVAANSCT ALLHLSLIAH NIGPGDEVIT TPMTFGATIN VIEHVGAKPI LVDLLPKSFH IDPQSIKSKI TPKTRAIMPV HYAGSPCQMD EINAIAKEYN LVVIEDAAHA IGTKYKGTLI GDGQNLTSFS LYVTKNIAAG EGGVLTTNDD DLAEKIRIYG LHGMSRGAWQ RYSKNSQIHY DIVVPGYKYN MPDMNAAMAI EQLKKLEIFT KKRKAIAEAY FEHLADENGL ELPHALIEQN EGDLCTWHIF PILINPEVLK GNREEIMAAL IQENIGVATH YRAIYEQQFY KEKYGLVPSD FPNCEMISKR IFSIPLSTSM STQDIEDVVS GIKKVFGYFK K

NT Sequence

atgtctaaac ttgccattca cggtggggag ccagttcgta aatctttctt agtctatggg gcgcctgata ttggagatga agaaattcaa gcggtcgtca attgtataaa aactggctgg ctcagtacag ggccgaatgt acaaaaattt gagactgata ttaagaacta cactggtgca aaacatgctg tagctgcaaa tagttgtact gctttgcttc atcttagttt aattgctcat aatataggtc cgggtgatga agtaatcact acccctatga cctttggtgc gacaataaat gtgatagaac atgtaggtgc taaaccgatt ttggtagact tattgcctaa aagttttcat atcgatcctc aatctataaa atccaaaatt acacctaaga ctagagctat catgccggtt cattatgcag gttctccctg tcaaatggat gaaattaacg cgattgcaaa agaatataac ttagttgtaa ttgaagatgc ggcgcatgca attggtacaa agtataaagg gactttgatc ggagatggcc aaaatcttac ttcatttagt ttgtatgtaa ccaaaaatat tgctgcaggg gaaggaggag tcttaactac gaatgatgat gaccttgcgg agaaaattcg tatttacgga ctccatggaa tgagtagagg tgcttggcaa cgttattcaa aaaattcgca aattcattat gatatagtag ttccgggata taaatataat atgcctgata tgaatgctgc aatggccatt gaacagttaa aaaaattgga aatttttact aaaaaaagaa aagcaatcgc agaagcttat tttgagcatc tggctgacga aaatggtctc gaattgcctc atgcgttgat tgaacaaaat gagggtgatc tatgcacttg gcatattttc ccaatcttaa ttaacccgga agttttaaaa ggaaatagag aagaaatcat ggccgccttg atccaagaaa atattggtgt agcgacccat tatcgagcta tctatgaaca acagttttat aaagagaagt atggcctcgt cccaagtgat tttcctaatt gtgaaatgat ctccaagagg atcttttcga ttccattgtc tacaagtatg tcgacacagg atattgaaga tgtcgtaagt ggaattaaaa aagtattcgg atattttaaa aaatag

Details for LpboA.17333.a.B1.GE41851

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/29/2017
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS KLAIHGGEPV RKSFLVYGAP DIGDEEIQAV VNCIKTGWLS TGPNVQKFET DIKNYTGAKH AVAANSCTAL LHLSLIAHNI GPGDEVITTP MTFGATINVI EHVGAKPILV DLLPKSFHID PQSIKSKITP KTRAIMPVHY AGSPCQMDEI NAIAKEYNLV VIEDAAHAIG TKYKGTLIGD GQNLTSFSLY VTKNIAAGEG GVLTTNDDDL AEKIRIYGLH GMSRGAWQRY SKNSQIHYDI VVPGYKYNMP DMNAAMAIEQ LKKLEIFTKK RKAIAEAYFE HLADENGLEL PHALIEQNEG DLCTWHIFPI LINPEVLKGN REEIMAALIQ ENIGVATHYR AIYEQQFYKE KYGLVPSDFP NCEMISKRIF SIPLSTSMST QDIEDVVSGI K

Validated NT Sequence

ttttaattcc acttacgaca tcttcaatat cctgtgtcga catacttgta gacaatggaa tcgaaaagat cctcttggag atcatttcac aattaggaaa atcacttggg acgaggccat acttctcttt ataaaactgt tgttcataga tagctcgata atgggtcgct acaccaatat tttcttggat caaggcggcc atgatttctt ctctatttcc ttttaaaact tccgggttaa ttaagattgg gaaaatatgc caagtgcata gatcaccctc attttgttca atcaacgcat gaggcaattc gagaccattt tcgtcagcca gatgctcaaa ataagcttct gcgattgctt ttcttttttt agtaaaaatt tccaattttt ttaactgttc aatggccatt gcagcattca tatcaggcat attatattta tatcccggaa ctactatatc ataatgaatt tgcgaatttt ttgaataacg ttgccaagca cctctactca ttccatggag tccgtaaata cgaattttct ccgcaaggtc atcatcattc gtagttaaga ctcctccttc ccctgcagca atatttttgg ttacatacaa actaaatgaa gtaagatttt ggccatctcc gatcaaagtc cctttatact ttgtaccaat tgcatgcgcc gcatcttcaa ttacaactaa gttatattct tttgcaatcg cgttaatttc atccatttga cagggagaac ctgcataatg aaccggcatg atagctctag tcttaggtgt aattttggat tttatagatt gaggatcgat atgaaaactt ttaggcaata agtctaccaa aatcggttta gcacctacat gttctatcac atttattgtc gcaccaaagg tcataggggt agtgattact tcatcacccg gacctatatt atgagcaatt aaactaagat gaagcaaagc agtacaacta tttgcagcta cagcatgttt tgcaccagtg tagttcttaa tatcagtctc aaatttttgt acattcggcc ctgtactgag ccagccagtt tttatacaat tgacgaccgc ttgaatttct tcatctccaa tatcaggcgc cccatagact aagaaagatt tacgaactgg ctccccaccg tgaatggcaa gtttagacat atggtggtgg tggtggtgag ccatggtnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta aacttgccat tcacggtggg gagccagttc gtaaatcttt cttagtctat ggggcgcctg atattggaga tgaagaaatt caagcggtcg tcaattgtat aaaaactggc tggctcagta cagggccgaa tgtacaaaaa tttgagactg atattaagaa ctacactggt gcaaaacatg ctgtagctgc aaatagttgt actgctttgc ttcatcttag tttaattgct cataatatag gtccgggtga tgaagtaatc actaccccta tgacctttgg tgcgacaata aatgtgatag aacatgtagg tgctaaaccg attttggtag acttattgcc taaaagtttt catatcgatc ctcaatctat aaaatccaaa attacaccta agactagagc tatcatgccg gttcattatg caggttctcc ctgtcaaatg gatgaaatta acgcgattgc aaaagaatat aacttagttg taattgaaga tgcggcgcat gcaattggta caaagtataa agggactttg atcggagatg gccaaaatct tacttcattt agtttgtatg taaccaaaaa tattgctgca ggggaaggag gagtcttaac tacgaatgat gatgaccttg cggagaaaat tcgtatttac ggactccatg gaatgagtag aggtgcttgg caacgttatt caaaaaattc gcaaattcat tatgatatag tagttccggg atataaatat aatatgcctg atatgaatgc tgcaatggcc attgaacagt taaaaaaatt ggaaattttt actaaaaaaa gaaaagcaat cgcagaagct tattttgagc atctggctga cgaaaatggt ctcgaattgc ctcatgcgtt gattgaacaa aatgagggtg atctatgcac ttggcatatt ttcccaatct taattaaccc ggaagtttta aaaggaaata gagaagaaat catggccgcc ttgatccaag aaaatattgg tgtagcgacc cattatcgag ctatctatga acaacagttt tataaagaga agtatggcct cgtcccaagt gattttccta attgtgaaat gatctccaag aggatctttt cgattccatt gtctacaagt atgtcgacac aggatattga agatgtcgta agtggaatta aaaaagtatt cggatatttt aaaaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t