LpboA.10611.a

Zinc-binding dehydrogenase

CENTER ID: LpboA.10611.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.10611.a.B2.GE41849 soluble domain #1 1 148
LpboA.10611.a.B3.GE41850 soluble domain #2 170 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1518
UniProt: Q04TN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRQKLKQESI IVELPEPQKI IFKIVELHLS EMREDEVYAE TIYSAISTGT ELSAYKGEKP IRPVSNPYPR VVGYCNVAEI KEVGSKVSLY MVGDYILTFQ SHRTSFICKE SDVILKIPKN GVSLQHAATT FLYHLGYETF LKSNFKPGFN VAVIGLGTLG LATVNIANLI GCKIFSFSNY PDNLKLAKEF GSHQSYLKNE NGIQKSIYEL TYGTGIDIVI TTSNTWDDWK LALELARDEG VIGVIGFPGR GQVLPDFNPL EPQYLYHKKL TVVYSGYMPD YLIPAKDIRF TRKRNCQFIL DQIIDNKLFP GKLIHKEYRW KDIETAYKDM LERKQPIYTS ILNWKK
NT Sequence
ttgaggcaaa aattgaaaca agaaagtata atagttgagt taccggaacc tcaaaaaata atttttaaaa ttgttgaatt acatctcagt gaaatgcgtg aagatgaagt ttatgcagaa acaatttact ctgctatttc tacgggaact gaattatctg catataaggg agaaaagccg attcggccag ttagtaatcc ttatcctaga gtagtcggat attgtaatgt tgcagaaatt aaggaagttg ggtccaaagt cagcctatat atggtgggag attatatact tacttttcaa tctcatagaa cttccttcat ttgtaaggaa tctgatgtta ttttaaaaat tcctaaaaac ggtgtttctt tgcaacatgc ggcgacaact ttcttatatc acctaggtta tgaaactttt ttaaaaagta attttaagcc aggatttaat gtcgctgtga ttggattggg aactttgggg ttggctacgg ttaatatcgc aaacttgatc ggatgtaaga ttttttcttt ctccaattat ccggataatc taaaattagc aaaagaattc ggttctcatc aatcttattt aaagaatgag aatggaattc agaaatctat ttatgaatta acatacggaa cggggattga tatagtgatt actacttcta atacttggga cgattggaaa ttagctttag agctggctag ggatgaggga gttatcggag ttatcggttt tcctggtcga ggtcaagtct tgcctgattt taatccgttg gaacctcagt atttatatca caaaaaacta acggtagttt actcaggtta tatgccagac tatttgattc cagctaagga tattcgtttt acccgaaaac gaaattgtca atttatattg gatcaaatca tcgataataa gttgtttcca ggaaagctga ttcataaaga atataggtgg aaggatatag aaactgctta caaggatatg ctagaaagaa aacaacccat ttatacttct atcctcaatt ggaaaaaatg a
Details for LpboA.10611.a.B2.GE41849
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML RQKLKQESII VELPEPQKII FKIVELHLSE MREDEVYAET IYSAISTGTE LSAYKGEKPI RPVSNPYPRV VGYCNVAEIK EVGSKVSLYM VGDYILTFQS HRTSFICKES DVILKIPKNG VSLQHAATTF LYHLGYETFL KSNFKPG
Validated NT Sequence
atcttactca cttatcctgg cttaaaatta ctttttaaaa aagtttcata acctaggtga tataagaaag ttgtcgccgc atgttgcaaa gaaacaccgt ttttaggaat ttttaaaata acatcagatt ccttacaaat gaaggaagtt ctatgagatt gaaaagtaag tatataatct cccaccatat ataggctgac tttggaccca acttccttaa tttctgcaac attacaatat ccgactactc taggataagg attactaact ggccgaatcg gcttttctcc cttatatgca gataattcag ttcccgtaga aatagcagag taaattgttt ctgcataaac ttcatcttca cgcatttcac tgagatgtaa ttcaacaatt ttaaaaatta ttttttgagg ttccggtaac tcaactatta tactttcttg tttcaatttt tgcctcaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMR QKLKQESIIV ELPEPQKIIF KIVELHLSEM REDEVYAETI YSAISTGTEL SAYKGEKPIR PVSNPYPRVV GYCNVAEIKE VGSKVSLYMV GDYILTFQSH RTSFICKESD VILKIPKNGV SLQHAATTFL YHLGYETFLK SNFKPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaggc aaaaattgaa acaagaaagt ataatagttg agttaccgga acctcaaaaa ataattttta aaattgttga attacatctc agtgaaatgc gtgaagatga agtttatgca gaaacaattt actctgctat ttctacggga actgaattat ctgcatataa gggagaaaag ccgattcggc cagttagtaa tccttatcct agagtagtcg gatattgtaa tgttgcagaa attaaggaag ttgggtccaa agtcagccta tatatggtgg gagattatat acttactttt caatctcata gaacttcctt catttgtaag gaatctgatg ttattttaaa aattcctaaa aacggtgttt ctttgcaaca tgcggcgaca actttcttat atcacctagg ttatgaaact tttttaaaaa gtaattttaa gccaggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LpboA.10611.a.B3.GE41850
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI GCKIFSFSNY PDNLKLAKEF GSHQSYLKNE NGIQKSIYEL TYGTGIDIVI TTSNTWDDWK LALELARDEG VIGVIGFPGR GQVLPDFNPL EPQYLYHKKL TVVYSGYMPD YLIPAKDIRF TRKRNCQFIL DQIIDNKLFP GKLIHKEYRW KDIETAYKDM LERKQPIYTS ILN
Validated NT Sequence
atcttactca cttaattgag gatagaagta taaatgggtt gttttctttc tagcatatcc ttgtaagcag tttctatatc cttccaccta tattctttat gaatcagctt tcctggaaac aacttattat cgatgatttg atccaatata aattgacaat ttcgttttcg ggtaaaacga atatccttag ctggaatcaa atagtctggc atataacctg agtaaactac cgttagtttt ttgtgatata aatactgagg ttccaacgga ttaaaatcag gcaagacttg acctcgacca ggaaaaccga taactccgat aactccctca tccctagcca gctctaaagc taatttccaa tcgtcccaag tattagaagt agtaatcact atatcaatcc ccgttccgta tgttaattca taaatagatt tctgaattcc attctcattc tttaaataag attgatgaga accgaattct tttgctaatt ttagattatc cggataattg gagaaagaaa aaatcttaca tccgatcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHIG CKIFSFSNYP DNLKLAKEFG SHQSYLKNEN GIQKSIYELT YGTGIDIVIT TSNTWDDWKL ALELARDEGV IGVIGFPGRG QVLPDFNPLE PQYLYHKKLT VVYSGYMPDY LIPAKDIRFT RKRNCQFILD QIIDNKLFPG KLIHKEYRWK DIETAYKDML ERKQPIYTSI LN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcggat gtaagatttt ttctttctcc aattatccgg ataatctaaa attagcaaaa gaattcggtt ctcatcaatc ttatttaaag aatgagaatg gaattcagaa atctatttat gaattaacat acggaacggg gattgatata gtgattacta cttctaatac ttgggacgat tggaaattag ctttagagct ggctagggat gagggagtta tcggagttat cggttttcct ggtcgaggtc aagtcttgcc tgattttaat ccgttggaac ctcagtattt atatcacaaa aaactaacgg tagtttactc aggttatatg ccagactatt tgattccagc taaggatatt cgttttaccc gaaaacgaaa ttgtcaattt atattggatc aaatcatcga taataagttg tttccaggaa agctgattca taaagaatat aggtggaagg atatagaaac tgcttacaag gatatgctag aaagaaaaca acccatttat acttctatcc tcaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat