SSGCID: Target Page

LpboA.01673.a

TolC-related efflux protein

CENTER ID:	LpboA.01673.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.01673.a.B1.GE41848	full length		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.811
UniProt:	Q04QB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLPSENAEP KNVCVKLGAL KPELRVTFYK LSFLKKSLEF FDENINSIRK TIKSSEAVYK NRDILLSEVL RLKSILFRLE TDRSEIVSDI IEKENILKLL LNEPNYSETI LEIETTDLDE KRNSILAKSD QELLSIAFEK RPDLKALEFG IKAEQTNLAL QRSLAIPDLT LGGSWDRAGN YINNYYGFTV SISIPTFDRN QGNIRESELI LASKKAKYEE KKSKSKPKFF PPSGKPKRKK GYS

NT Sequence

gtgcctctgc cttccgaaaa tgcagaacca aaaaacgttt gcgtaaaact cggagcgctc aaaccggaat tgagagtcac attttataag ttatcctttc tgaaaaaatc ccttgaattt ttcgatgaaa acatcaattc cattcgaaag acgattaaaa gttcggaagc ggtctataaa aatcgggaca tcttgctctc cgaagtccta agactcaaat ccattctgtt tcgactcgaa acggaccgct ctgaaatagt aagcgacatt atagaaaaag agaatattct aaaattatta ctcaacgaac cgaattattc ggaaacgatc ctcgaaatcg aaactaccga tctagacgaa aaacgaaatt cgatccttgc caaaagcgac caggaactcc tttccatcgc ttttgaaaaa agacccgatc ttaaagcctt ggaatttgga atcaaggcag aacaaacgaa cctcgctctc caaaggtctt tggcaattcc ggatttaaca ctcggaggaa gttgggatcg cgcgggaaac tacatcaata attactacgg ttttacggtt tccatatcga ttcccacatt cgatcgtaat caaggaaaca ttcgggaatc ggaattgatt ctcgcctcaa aaaaagcgaa gtacgaggaa aaaaaatcaa aatcaaagcc gaagttcttt ccgccgtcgg gaaagccaaa gagaaagaaa ggttactctt ag

Details for LpboA.01673.a.B1.GE41848

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV PLPSENAEPK NVCVKLGALK PELRVTFYKL SFLKKSLEFF DENINSIRKT IKSSEAVYKN RDILLSEVLR LKSILFRLET DRSEIVSDII EKENILKLLL NEPNYSETIL EIETTDLDEK RNSILAKSDQ ELLSIAFEKR PDLKALEFGI KAEQTNLALQ RSLAIPDLTL GGSWDRAGNY INNYYGFTVS ISIPTFDRNQ GNIRESELIL ASKKAKYEEK KSKSKPKFFP PSGXXXRKKG YS

Validated NT Sequence

atcttactca cttaagagta acctttcttt ctnnnnnnnn ttcccgacgg cggaaagaac ttcggctttg attttgattt tttttcctcg tacttcgctt tttttgaggc gagaatcaat tccgattccc gaatgtttcc ttgattacga tcgaatgtgg gaatcgatat ggaaaccgta aaaccgtagt aattattgat gtagtttccc gcgcgatccc aacttcctcc gagtgttaaa tccggaattg ccaaagacct ttggagagcg aggttcgttt gttctgcctt gattccaaat tccaaggctt taagatcggg tcttttttca aaagcgatgg aaaggagttc ctggtcgctt ttggcaagga tcgaatttcg tttttcgtct agatcggtag tttcgatttc gaggatcgtt tccgaataat tcggttcgtt gagtaataat tttagaatat tctctttttc tataatgtcg cttactattt cagagcggtc cgtttcgagt cgaaacagaa tggatttgag tcttaggact tcggagagca agatgtcccg atttttatag accgcttccg aacttttaat cgtctttcga atggaattga tgttttcatc gaaaaattca agggattttt tcagaaagga taacttataa aatgtgactc tcaattccgg tttgagcgct ccgagtttta cgcaaacgtt ttttggttct gcattttcgg aaggcagagg caccatatgg tggtggtggt ggtgagccat ggtat

Expected Protein Sequence

MAHHHHHHMP LPSENAEPKN VCVKLGALKP ELRVTFYKLS FLKKSLEFFD ENINSIRKTI KSSEAVYKNR DILLSEVLRL KSILFRLETD RSEIVSDIIE KENILKLLLN EPNYSETILE IETTDLDEKR NSILAKSDQE LLSIAFEKRP DLKALEFGIK AEQTNLALQR SLAIPDLTLG GSWDRAGNYI NNYYGFTVSI SIPTFDRNQG NIRESELILA SKKAKYEEKK SKSKPKFFPP SGKPKRKKGY S

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcctc tgccttccga aaatgcagaa ccaaaaaacg tttgcgtaaa actcggagcg ctcaaaccgg aattgagagt cacattttat aagttatcct ttctgaaaaa atcccttgaa tttttcgatg aaaacatcaa ttccattcga aagacgatta aaagttcgga agcggtctat aaaaatcggg acatcttgct ctccgaagtc ctaagactca aatccattct gtttcgactc gaaacggacc gctctgaaat agtaagcgac attatagaaa aagagaatat tctaaaatta ttactcaacg aaccgaatta ttcggaaacg atcctcgaaa tcgaaactac cgatctagac gaaaaacgaa attcgatcct tgccaaaagc gaccaggaac tcctttccat cgcttttgaa aaaagacccg atcttaaagc cttggaattt ggaatcaagg cagaacaaac gaacctcgct ctccaaaggt ctttggcaat tccggattta acactcggag gaagttggga tcgcgcggga aactacatca ataattacta cggttttacg gtttccatat cgattcccac attcgatcgt aatcaaggaa acattcggga atcggaattg attctcgcct caaaaaaagc gaagtacgag gaaaaaaaat caaaatcaaa gccgaagttc tttccgccgt cgggaaagcc aaagagaaag aaaggttact cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat