SSGCID: Target Page

LpboA.01664.a

Sensor histidine kinase and response regulator of a two component complex

CENTER ID:	LpboA.01664.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.01664.a.B2.GE41847	soluble domain		176	787

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.3479
UniProt:	Q04PQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVFSDTEKD FSVRLMYGIM LSCGAVIISY RTLHPIFAEK PKGAYAATYL ILAAIITCHL IAKSGRILLA SHVLIGIEWL AAFYGMIGEG ATQTVVFPFC LSLILISALL LGIRAAYFYT FLAIAVELTS TYLIRIEVIR PVKSTGPWSS LIGEIGSFIL VAILMRYTLF GFRIVKTELS EVQRYAKLGG WSLNIQTLEL VLSKEYQYLL GYDDAKESKT LHLSTFLNTY VVDEKDKARI LDILAKSRTQ KEITDCTVEF VYQIRRKDGQ HRFLAGKGKF RDSTIGIGTA QDITERHLAE EALRPAQEIY SKVFTLSPIA TTISSAHDGR YVEVNDSFLN LFGFTREETI GKTSIELNIW PDPAQRKAYF DKLSQKNVLV DEELIFQGKN GKIIHAECYS TLAEINGKLC AINLVKDISE KKEAESLRKL NKEISDQNKL IERQKQELEE ILKDLKKNQN QLIISEKMAS LGQLAAGIAH EINNPIGVVK SANDSIQNYF KSSTDRIIQV SETLQGLNVV VLNELSTFVK KGKKNREILS PKEIREKIKR LEVKLKVKGF ENPRSVAQDL AELGLDSSVE EFPNLFTHKD GQVLLRFAIA EIQAFQNSKL IDISVNRTSK VVYALKKFTH WSSEGIKIPV VISESIETVL TIYHNQLKIG VEINREYKDI PTIQAYADDL IHLWTNLIYN AVQAMSFKGI LGIRISRVGE NEVEVVVSDT GPGISAFAKE RIFEPFFTTK APGEGSGMGL DIAKKIVETH EGTIRFETSP QGTSFFVRLP ISETNTN

NT Sequence

atgcctgttt tttcggatac tgagaaggat ttttccgtac ggttgatgta cggaattatg ctctcttgcg gggcggtaat cataagctat cgcactctgc atccgatctt tgccgaaaaa cctaaagggg cttacgcggc aacgtatctg atcctcgctg cgattatcac ttgtcactta attgcaaaaa gcggaagaat tcttttagcc tctcatgttc taatcggaat cgaatggttg gccgcattct atggcatgat aggggaaggt gccactcaaa cggtagtttt tccattttgt ttgagcttaa ttttgatctc cgcacttctg ttgggaatca gggccgctta tttttacaca ttcctagcga ttgcagtcga gcttacaagt acctatctca ttcggatcga agtgattcgg cccgttaaaa gtactggacc ttggtcctct ttgatcggag aaattggctc ttttatactc gtagcaattt tgatgagata cactttgttc ggatttagaa tcgtaaaaac tgaattgtcc gaagttcaac gttatgcgaa attaggagga tggagtctga atatacaaac tttagaactt gttctctcta aagaatacca atacttgctc gggtacgacg atgcgaagga atccaaaacc ttacacttgt ccacgttctt aaatacttat gtcgtcgatg aaaaagataa ggctcgtatc ctcgacattc tcgcaaaaag ccggacacaa aaagaaatta cggattgcac cgtagagttc gtttatcaaa tccgaagaaa agacggacaa catcgattcc ttgctggaaa gggaaaattt agagactcga ccatcggtat cggaacggcc caagacatca cggaaagaca tctagcggaa gaggctcttc gtcccgctca ggaaatttat tcgaaggtgt ttaccctcag tccgattgcg acaacgattt catccgcgca tgatggaaga tacgtcgaag tcaacgatag ttttttgaac ttgttcggat ttacgagaga agaaacaatc ggaaagacga gtatagaatt gaacatttgg cccgatccgg ctcaaagaaa agcatatttc gataaattgt ctcaaaagaa cgtactcgtg gatgaggagt tgattttcca aggtaaaaac ggaaaaatta ttcacgcgga atgttatagc acattagctg aaatcaatgg aaaactttgt gcgatcaatc ttgtaaaaga tatttcggaa aaaaaagaag cggaatctct gagaaaattg aataaggaaa tctccgatca aaataaattg atcgaaaggc aaaagcaaga actcgaagaa attctgaagg atctgaaaaa aaaccaaaat caattgatca tatccgaaaa gatggcctca ttaggtcaac tagcggccgg aattgcgcat gaaatcaaca atcccatcgg agttgtgaag tcggcgaatg attccataca gaattatttt aaaagttcca cagatagaat cattcaggtc tccgaaaccc tccagggatt aaatgtagtg gtattgaacg aactcagtac gttcgtaaaa aagggaaaaa aaaatcggga aatcctatct cctaaggaaa ttcgcgagaa aataaaaagg cttgaagtaa aattgaaagt aaaaggattc gaaaatcctc gttcggttgc gcaagacttg gcggaacttg gtttggattc ttccgtggaa gaatttccaa atctgtttac gcataaggac ggacaggtac tattacgttt tgcgatcgcg gaaattcagg cctttcaaaa ttccaagttg atcgacatat ctgtgaatcg aacttccaaa gtcgtttatg ctctcaaaaa attcacccac tggagttcgg aaggaatcaa aatcccggtg gtaatttcgg aaagtataga aaccgttctt acgatttatc ataatcaact gaaaattgga gtggaaatca atagggaata taaagatatt ccgactattc aagcttatgc ggatgatctc atccatctat ggacgaattt gatctacaac gccgtacaag caatgtcctt caaagggatt ttagggattc gaatttctcg tgtgggagaa aacgaagtgg aagttgtcgt ttcggatacg ggacccggaa tttccgcatt tgcgaaagaa agaattttcg aacctttttt cactacaaag gctccagggg aaggatcggg aatgggtttg gacatcgcaa agaaaatcgt agaaacgcat gaaggaacaa tccgatttga aacttctccc caaggaactt ctttttttgt aagacttccg atctccgaaa cgaacacaaa ctaa

Details for LpboA.01664.a.B2.GE41847

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHKT ELSEVQRYAK LGGWSLNIQT LELVLSKEYQ YLLGYDDAKE SKTLHLSTFL NTYVVDEKDK ARILDILAKS RTQKEITDCT VEFVYQIRRK DGQHRFLAGK GKFRDSTIGI GTAQDITERH LAEEALRPAQ EIYSKVFTLS PIATTISSAH DGRYVEVNDS FLNLFGFTRE ETIGKTSIEL NIWPDPAQRK AYFDKLSQKN VLVDEELIFQ GKNGKIIHAE CYSTLAEING KLCAINLVKD ISEKKEAESL RKLNKEISDQ NKLIERQKQE LEEILKDLKK NQNQLIISEK MASLGQLAAG IAHEINNPIG VVKSANDSIQ NYFKSSTDRI IQVSETLQGL NVVVLNELST FVKKGKKNRE ILSPKEIREK IKRLEVKLKV KGFENPRSVA QDLAELGLDS SVEEFPNLFT HKDGQVLLRF AIAEIQAFQN SKLIDISVNR TSKVVYALKK FTHWSSEGIK IPVVISESIE TVLTIYHNQL KIGVEINREY KDIPTIQAYA DDLIHLWTNL IYNAVQAMSF KGILGIRISR VGENEVEVVV SDTGPGISAF AKERIFEPFF TTKAPGEGSG MGLDIAKKIV ETHEGTIRFE TSPQGTSFFV RLPISETNTN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaactg aattgtccga agttcaacgt tatgcgaaat taggaggatg gagtctgaat atacaaactt tagaacttgt tctctctaaa gaataccaat acttgctcgg gtacgacgat gcgaaggaat ccaaaacctt acacttgtcc acgttcttaa atacttatgt cgtcgatgaa aaagataagg ctcgtatcct cgacattctc gcaaaaagcc ggacacaaaa agaaattacg gattgcaccg tagagttcgt ttatcaaatc cgaagaaaag acggacaaca tcgattcctt gctggaaagg gaaaatttag agactcgacc atcggtatcg gaacggccca agacatcacg gaaagacatc tagcggaaga ggctcttcgt cccgctcagg aaatttattc gaaggtgttt accctcagtc cgattgcgac aacgatttca tccgcgcatg atggaagata cgtcgaagtc aacgatagtt ttttgaactt gttcggattt acgagagaag aaacaatcgg aaagacgagt atagaattga acatttggcc cgatccggct caaagaaaag catatttcga taaattgtct caaaagaacg tactcgtgga tgaggagttg attttccaag gtaaaaacgg aaaaattatt cacgcggaat gttatagcac attagctgaa atcaatggaa aactttgtgc gatcaatctt gtaaaagata tttcggaaaa aaaagaagcg gaatctctga gaaaattgaa taaggaaatc tccgatcaaa ataaattgat cgaaaggcaa aagcaagaac tcgaagaaat tctgaaggat ctgaaaaaaa accaaaatca attgatcata tccgaaaaga tggcctcatt aggtcaacta gcggccggaa ttgcgcatga aatcaacaat cccatcggag ttgtgaagtc ggcgaatgat tccatacaga attattttaa aagttccaca gatagaatca ttcaggtctc cgaaaccctc cagggattaa atgtagtggt attgaacgaa ctcagtacgt tcgtaaaaaa gggaaaaaaa aatcgggaaa tcctatctcc taaggaaatt cgcgagaaaa taaaaaggct tgaagtaaaa ttgaaagtaa aaggattcga aaatcctcgt tcggttgcgc aagacttggc ggaacttggt ttggattctt ccgtggaaga atttccaaat ctgtttacgc ataaggacgg acaggtacta ttacgttttg cgatcgcgga aattcaggcc tttcaaaatt ccaagttgat cgacatatct gtgaatcgaa cttccaaagt cgtttatgct ctcaaaaaat tcacccactg gagttcggaa ggaatcaaaa tcccggtggt aatttcggaa agtatagaaa ccgttcttac gatttatcat aatcaactga aaattggagt ggaaatcaat agggaatata aagatattcc gactattcaa gcttatgcgg atgatctcat ccatctatgg acgaatttga tctacaacgc cgtacaagca atgtccttca aagggatttt agggattcga atttctcgtg tgggagaaaa cgaagtggaa gttgtcgttt cggatacggg acccggaatt tccgcatttg cgaaagaaag aattttcgaa ccttttttca ctacaaaggc tccaggggaa ggatcgggaa tgggtttgga catcgcaaag aaaatcgtag aaacgcatga aggaacaatc cgatttgaaa cttctcccca aggaacttct ttttttgtaa gacttccgat ctccgaaacg aacacaaact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat