LpboA.01042.a

Aryl-alcohol dehydrogenase-related oxidoreductase

CENTER ID: LpboA.01042.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.01042.a.B1.GE41844 full length 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1526
UniProt: Q04TM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGIEEEDYIT LPKTDLKVSR LSIGTWLFGG LRWGYVDNRE SEKTFLSALD LGINFIDTAD AYGKGQSEEF VSRLTKGRKE NLVIGTKAGV VWNTDGTRRI DLSKRHLTEA AERSLQRLKL DKIDLYYLHE PDVNTDIEET IEALQLLKAQ GKIRYIGLSN FPKETVAQFN SMMHVSCLQD ELSLIRREAE LTNLKFAKEN NLGFLAYSPL SKGLLTGKFK FDSQFPSDDN RSGNEDFSGE RLKKNIEKVA ILSQFAKRLG YTASAVSIAW VLGIDSVTSV VLGARTQEQL EEQMKSLEVV FELDAYQQVE RIFS
NT Sequence
ttggggattg aagaagaaga ttatatcact ctacctaaaa ccgatctcaa agtttctcga ttgagcatag gtacctggtt atttggaggt cttcgttggg gttatgtaga caatcgggaa agtgaaaaaa cttttttatc tgctctcgat cttggaatca attttatcga tacggctgat gcctatggaa aggggcagtc agaggaattt gtctctcgat tgacaaaagg tagaaaagaa aatttagtaa ttgggacaaa ggcaggggtt gtttggaata cggatggcac aagaaggatt gatttatcta aaagacatct taccgaagcg gcggaaagaa gtttgcaaag acttaaatta gacaagattg atttatatta tttgcatgaa ccagatgtaa atacagatat agaagaaaca atagaagcgt tgcaactatt aaaagctcaa ggtaaaattc gttatattgg cctttctaat tttccgaaag aaaccgttgc tcagtttaat tcgatgatgc acgtttcctg cttgcaggac gagcttagtt taattcgcag agaagccgaa ttaacgaatc tgaaattcgc aaaagaaaat aatttaggat ttttggctta tagccctttg tcaaaaggac ttcttacggg aaagtttaaa ttcgatagtc aattcccttc tgacgacaat cgctcgggga atgaagattt ttcaggtgaa agattgaaga agaatattga gaaagtagct atactttctc aatttgcaaa aagactagga tatacggcat cggccgtttc tattgcttgg gttttaggta tagattcagt aacgtctgtt gtcttaggtg ccagaacgca ggaacaattg gaagagcaga tgaagagtct cgaagttgtt tttgagttag atgcatatca gcaagtagag cggattttta gttaa
Details for LpboA.01042.a.B1.GE41844
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML GIEEEDYITL PKTDLKVSRL SIGTWLFGGL RWGYVDNRES EKTFLSALDL GINFIDTADA YGKGQSEEFV SRLTKGRKEN LVIGTKAGVV WNTDGTRRID LSKRHLTEAA ERSLQRLKLD KIDLYYLHEP DVNTDIEETI EALQLLKAQG KIRYIGLSNF PKETVAQFNS MMHVSCLQDE LSLIRREAEL TNLKFAKENN LGFLAYSPLS KGLLTGKFKF DSQFPSDDNR SGNEDFSGER LKKNIEKVAI LSQFAKRLGY TASAVSIAWV LGIDSVTSVV LGARTQEQLE EQMKSLEVVF ELDAXXXVER IFS
Validated NT Sequence
tcacttaact aaaaatccgc tctacttnnn nnnatgcatc taactcaaaa acaacttcga gactcttcat ctgctcttcc aattgttcct gcgttctggc acctaagaca acagacgtta ctgaatctat acctaaaacc caagcaatag aaacggccga tgccgtatat cctagtcttt ttgcaaattg agaaagtata gctactttct caatattctt cttcaatctt tcacctgaaa aatcttcatt ccccgagcga ttgtcgtcag aagggaattg actatcgaat ttaaactttc ccgtaagaag tccttttgac aaagggctat aagccaaaaa tcctaaatta ttttcttttg cgaatttcag attcgttaat tcggcttctc tgcgaattaa actaagctcg tcctgcaagc aggaaacgtg catcatcgaa ttaaactgag caacggtttc tttcggaaaa ttagaaaggc caatataacg aattttacct tgagctttta atagttgcaa cgcttctatt gtttcttcta tatctgtatt tacatctggt tcatgcaaat aatataaatc aatcttgtct aatttaagtc tttgcaaact tctttccgcc gcttcggtaa gatgtctttt agataaatca atccttcttg tgccatccgt attccaaaca acccctgcct ttgtcccaat tactaaattt tcttttctac cttttgtcaa tcgagagaca aattcctctg actgcccctt tccataggca tcagccgtat cgataaaatt gattccaaga tcgagagcag ataaaaaagt tttttcactt tcccgattgt ctacataacc ccaacgaaga cctccaaata accaggtacc tatgctcaat cgagaaactt tgagatcggt tttaggtaga gtgatataat cttcttcttc aatccccaac atatggtggt ggtggtggtg agccatggta ta
Expected Protein Sequence
MAHHHHHHMG IEEEDYITLP KTDLKVSRLS IGTWLFGGLR WGYVDNRESE KTFLSALDLG INFIDTADAY GKGQSEEFVS RLTKGRKENL VIGTKAGVVW NTDGTRRIDL SKRHLTEAAE RSLQRLKLDK IDLYYLHEPD VNTDIEETIE ALQLLKAQGK IRYIGLSNFP KETVAQFNSM MHVSCLQDEL SLIRREAELT NLKFAKENNL GFLAYSPLSK GLLTGKFKFD SQFPSDDNRS GNEDFSGERL KKNIEKVAIL SQFAKRLGYT ASAVSIAWVL GIDSVTSVVL GARTQEQLEE QMKSLEVVFE LDAYQQVERI FS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgggga ttgaagaaga agattatatc actctaccta aaaccgatct caaagtttct cgattgagca taggtacctg gttatttgga ggtcttcgtt ggggttatgt agacaatcgg gaaagtgaaa aaactttttt atctgctctc gatcttggaa tcaattttat cgatacggct gatgcctatg gaaaggggca gtcagaggaa tttgtctctc gattgacaaa aggtagaaaa gaaaatttag taattgggac aaaggcaggg gttgtttgga atacggatgg cacaagaagg attgatttat ctaaaagaca tcttaccgaa gcggcggaaa gaagtttgca aagacttaaa ttagacaaga ttgatttata ttatttgcat gaaccagatg taaatacaga tatagaagaa acaatagaag cgttgcaact attaaaagct caaggtaaaa ttcgttatat tggcctttct aattttccga aagaaaccgt tgctcagttt aattcgatga tgcacgtttc ctgcttgcag gacgagctta gtttaattcg cagagaagcc gaattaacga atctgaaatt cgcaaaagaa aataatttag gatttttggc ttatagccct ttgtcaaaag gacttcttac gggaaagttt aaattcgata gtcaattccc ttctgacgac aatcgctcgg ggaatgaaga tttttcaggt gaaagattga agaagaatat tgagaaagta gctatacttt ctcaatttgc aaaaagacta ggatatacgg catcggccgt ttctattgct tgggttttag gtatagattc agtaacgtct gttgtcttag gtgccagaac gcaggaacaa ttggaagagc agatgaagag tctcgaagtt gtttttgagt tagatgcata tcagcaagta gagcggattt ttagttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat