LpboA.00107.a

Membrane efflux protein

CENTER ID:	LpboA.00107.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.00107.a.B2.GE41841	mature protein		35	476

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.00107.a.B2.PS38472	mature protein		35	476

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_2694

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|355276.3.peg.1223
UniProt:	Q04R83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFDPMKFRSY SYCRVFRKCI WIVILLFLFT DCKKSEKKEE VLPLEDRIKL AKIDPGESSS GLKVLGSVSF FKKAEVTSKI LGRIQQYFKE EGDSVLAGDV LAKMETLNLE IQLSKDQSSV EVQTRQRDLA SAKLLIAKQR VDRELANIEK AEADVRDSRE IRNNLLRSAE NKKKLNEAGA VSETELKSVE TSLNSAEISL FKAEKNLASL KMGYRPEDLN KANIQIPKNK SELKEAYIKL NTMIENAELD MAEANFKSTL KNMESTQLLL KESNIKSPLS GTVASRIKEQ GEAVKGGEAL YIVVDTSKVI LKFNIGESEL WKLKKDQKVN FIVDAYPGQN FEGKVYIISP IVDPQSRTVE VKVIAQNEKK LLKPGMFTRG YILLEEARKK TFLIPLKALI KNKEGKSGTV FVANEENRLF SVEVEIIKEE SGFVEVLGNL ELGRYIAISD LNELKEGQKV KIPKEQTPSL IEEKSY

NT Sequence

atgtttgatc ccatgaaatt cagatcatat tcttattgta gggtttttcg aaagtgtatc tggattgtaa tattactatt tctttttacg gattgcaaaa aaagcgagaa aaaggaggaa gtattacctc ttgaagatcg gattaaactc gccaagattg atccgggtga atcaagttcc ggccttaaag ttttaggttc cgtttctttt ttcaagaagg cggaggttac ttccaaaatt ttagggagaa ttcaacaata ttttaaagaa gagggagatt ccgttcttgc cggagatgtt cttgcaaaaa tggaaacgtt gaatttagaa attcaattat ctaaagatca atccagtgtg gaagtacaaa cccgtcaaag ggacctggcg tccgccaaac tactcatcgc taaacaaagg gtggatcggg aattggcgaa tatcgaaaag gcggaggcag acgtgaggga ctcgagagag attcggaata atcttctgcg cagcgcagaa aacaaaaaga aactgaacga agccggagcg gtttctgaaa cggaattgaa atccgtggaa acatctttga actccgccga aatttccctt tttaaagcgg agaaaaatct cgccagtcta aaaatgggtt atagaccgga agatttaaat aaagcgaata ttcaaattcc taaaaataag tcagaattaa aggaggctta tataaaatta aatactatga ttgaaaacgc cgaattggac atggccgagg caaactttaa atcgacgctt aaaaacatgg aatccacgca actcctatta aaggaatcga atattaaaag tccgttgtct ggaacggttg cttccagaat taaggaacag ggagaagccg tcaaaggggg agaggctttg tatatcgttg tcgacacctc taaagtaatc ttaaagttta acatcggtga atcggagctg tggaaactga aaaaagatca gaaagtgaat tttatcgttg atgcttatcc gggacaaaac tttgaaggaa aggtttatat catcagcccg attgtagatc cgcagagtag gacggtggaa gtaaaggtta tcgctcaaaa tgaaaaaaaa cttttaaaac cgggtatgtt cacacgtggt tatatccttc tagaagaggc tcgaaaaaaa acctttttaa ttccgctcaa agctctcatt aagaataagg aaggtaaaag cggaaccgtc tttgttgcaa acgaagaaaa tcggttgttt agcgtggagg tggaaatcat taaagaggaa agcggttttg tggaagtatt aggaaatctt gaattaggac gatatattgc gatttccgat ttgaatgaac tcaaggaagg tcaaaaagtt aaaattccaa aggaacaaac tcccagtttg atcgaagaga aatcttattg a

Details for LpboA.00107.a.B2.GE41841

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	51kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHMSE KKEEVLPLED RIKLAKIDPG ESSSGLKVLG SVSFFKKAEV TSKILGRIQQ YFKEEGDSVL AGDVLAKMET LNLEIQLSKD QSSVEVQTRQ RDLASAKLLI AKQRVDRELA NIEKAEADVR DSREIRNNLL RSAENKKKLN EAGAVSETEL KSVETSLNSA EISLFKAEKN LASLKMGYRP EDLNKANIQI PKNKSELKEA YIKLNTMIEN AELDMAEANF KSTLKNMEST QLLLKESNIK SPLSGTVASR IKEQGEAVKG GEALYIVVDT SKVILKFNIG ESELWKLKKD QKVNFIVDAY PGQNFEGKVY IISPIVDPQS RTVEVKVIAQ NEKKLLKPGM FTRGYILLEE ARKKTFLIPL KALIKNKEGK SGTVFVANEE NRLFSVEVEI IKEESGFVEV LGNLELGRYI AISDLNELKE GQKVKIPKEQ TP

Validated NT Sequence

tgggagtttg ttcctttgga attttaactt tttgaccttc cttgagttca ttcaaatcgg aaatcgcaat atatcgtcct aattcaagat ttcctaatac ttccacaaaa ccgctttcct ctttaatgat ttccacctcc acgctaaaca accgattttc ttcgtttgca acaaagacgg ttccgctttt accttcctta ttcttaatga gagctttgag cggaattaaa aaggtttttt ttcgagcctc ttctagaagg atataaccac gtgtgaacat acccggtttt aaaagttttt tttcattttg agcgataacc tttacttcca ccgtcctact ctgcggatct acaatcgggc tgatgatata aacctttcct tcaaagtttt gtcccggata agcatcaacg ataaaattca ctttctgatc ttttttcagt ttccacagct ccgattcacc gatgttaaac tttaagatta ctttagaggt gtcgacaacg atatacaaag cctctccccc tttgacggct tctccctgtt ccttaattct ggaagcaacc gttccagaca acggactttt aatattcgat tcctttaata ggagttgcgt ggattccatg tttttaagcg tcgatttaaa gtttgcctcg gccatgtcca attcggcgtt ttcaatcata gtatttaatt ttatataagc ctcctttaat tctgacttat ttttaggaat ttgaatattc gctttattta aatcttccgg tctataaccc atttttagac tggcgagatt tttctccgct ttaaaaaggg aaatttcggc ggagttcaaa gatgtttcca cggatttcaa ttccgtttca gaaaccgctc cggcttcgtt cagtttcttt ttgttttctg cgctgcgcag aagattattc cgaatctctc tcgagtccct cacgtctgcc tccgcctttt cgatattcgc caattcccga tccacccttt gtttagcgat gagtagtttg gcggacgcca ggtccctttg acgggtttgt acttccacac tggattgatc tttagataat tgaatttcta aattcaacgt ttccattttt gcaagaacat ctccggcaag aacggaatct ccctcttctt taaaatattg ttgaattctc cctaaaattt tggaagtaac ctccgccttc ttgaaaaaag aaacggaacc taaaacttta aggccggaac ttgattcacc cggatcaatc ttggcgagtt taatccgatc ttcaagaggt aatacttcct cctttttctc gctcatatgg tggtggtggt gagccatggt a

Expected Protein Sequence

MAHHHHHHSE KKEEVLPLED RIKLAKIDPG ESSSGLKVLG SVSFFKKAEV TSKILGRIQQ YFKEEGDSVL AGDVLAKMET LNLEIQLSKD QSSVEVQTRQ RDLASAKLLI AKQRVDRELA NIEKAEADVR DSREIRNNLL RSAENKKKLN EAGAVSETEL KSVETSLNSA EISLFKAEKN LASLKMGYRP EDLNKANIQI PKNKSELKEA YIKLNTMIEN AELDMAEANF KSTLKNMEST QLLLKESNIK SPLSGTVASR IKEQGEAVKG GEALYIVVDT SKVILKFNIG ESELWKLKKD QKVNFIVDAY PGQNFEGKVY IISPIVDPQS RTVEVKVIAQ NEKKLLKPGM FTRGYILLEE ARKKTFLIPL KALIKNKEGK SGTVFVANEE NRLFSVEVEI IKEESGFVEV LGNLELGRYI AISDLNELKE GQKVKIPKEQ TPSLIEEKSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagcgaga aaaaggagga agtattacct cttgaagatc ggattaaact cgccaagatt gatccgggtg aatcaagttc cggccttaaa gttttaggtt ccgtttcttt tttcaagaag gcggaggtta cttccaaaat tttagggaga attcaacaat attttaaaga agagggagat tccgttcttg ccggagatgt tcttgcaaaa atggaaacgt tgaatttaga aattcaatta tctaaagatc aatccagtgt ggaagtacaa acccgtcaaa gggacctggc gtccgccaaa ctactcatcg ctaaacaaag ggtggatcgg gaattggcga atatcgaaaa ggcggaggca gacgtgaggg actcgagaga gattcggaat aatcttctgc gcagcgcaga aaacaaaaag aaactgaacg aagccggagc ggtttctgaa acggaattga aatccgtgga aacatctttg aactccgccg aaatttccct ttttaaagcg gagaaaaatc tcgccagtct aaaaatgggt tatagaccgg aagatttaaa taaagcgaat attcaaattc ctaaaaataa gtcagaatta aaggaggctt atataaaatt aaatactatg attgaaaacg ccgaattgga catggccgag gcaaacttta aatcgacgct taaaaacatg gaatccacgc aactcctatt aaaggaatcg aatattaaaa gtccgttgtc tggaacggtt gcttccagaa ttaaggaaca gggagaagcc gtcaaagggg gagaggcttt gtatatcgtt gtcgacacct ctaaagtaat cttaaagttt aacatcggtg aatcggagct gtggaaactg aaaaaagatc agaaagtgaa ttttatcgtt gatgcttatc cgggacaaaa ctttgaagga aaggtttata tcatcagccc gattgtagat ccgcagagta ggacggtgga agtaaaggtt atcgctcaaa atgaaaaaaa acttttaaaa ccgggtatgt tcacacgtgg ttatatcctt ctagaagagg ctcgaaaaaa aaccttttta attccgctca aagctctcat taagaataag gaaggtaaaa gcggaaccgt ctttgttgca aacgaagaaa atcggttgtt tagcgtggag gtggaaatca ttaaagagga aagcggtttt gtggaagtat taggaaatct tgaattagga cgatatattg cgatttccga tttgaatgaa ctcaaggaag gtcaaaaagt taaaattcca aaggaacaaa ctcccagttt gatcgaagag aaatcttatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for LpboA.00107.a.B2.PS38472

PURIFICATION DATe:	7/3/2018
CONCENTRATION:	26.28mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGCGAGA AAAAGGAGGA AGTATTACCT CTTGAAGATC GGATTAAACT CGCCAAGATT GATCCGGGTG AATCAAGTTC CGGCCTTAAA GTTTTAGGTT CCGTTTCTTT TTTCAAGAAG GCGGAGGTTA CTTCCAAAAT TTTAGGGAGA ATTCAACAAT ATTTTAAAGA AGAGGGAGAT TCCGTTCTTG CCGGAGATGT TCTTGCAAAA ATGGAAACGT TGAATTTAGA AATTCAATTA TCTAAAGATC AATCCAGTGT GGAAGTACAA ACCCGTCAAA GGGACCTGGC GTCCGCCAAA CTACTCATCG CTAAACAAAG GGTGGATCGG GAATTGGCGA ATATCGAAAA GGCGGAGGCA GACGTGAGGG ACTCGAGAGA GATTCGGAAT AATCTTCTGC GCAGCGCAGA AAACAAAAAG AAACTGAACG AAGCCGGAGC GGTTTCTGAA ACGGAATTGA AATCCGTGGA AACATCTTTG AACTCCGCCG AAATTTCCCT TTTTAAAGCG GAGAAAAATC TCGCCAGTCT AAAAATGGGT TATAGACCGG AAGATTTAAA TAAAGCGAAT ATTCAAATTC CTAAAAATAA GTCAGAATTA AAGGAGGCTT ATATAAAATT AAATACTATG ATTGAAAACG CCGAATTGGA CATGGCCGAG GCAAACTTTA AATCGACGCT TAAAAACATG GAATCCACGC AACTCCTATT AAAGGAATCG AATATTAAAA GTCCGTTGTC TGGAACGGTT GCTTCCAGAA TTAAGGAACA GGGAGAAGCC GTCAAAGGGG GAGAGGCTTT GTATATCGTT GTCGACACCT CTAAAGTAAT CTTAAAGTTT AACATCGGTG AATCGGAGCT GTGGAAACTG AAAAAAGATC AGAAAGTGAA TTTTATCGTT GATGCTTATC CGGGACAAAA CTTTGAAGGA AAGGTTTATA TCATCAGCCC GATTGTAGAT CCGCAGAGTA GGACGGTGGA AGTAAAGGTT ATCGCTCAAA ATGAAAAAAA ACTTTTAAAA CCGGGTATGT TCACACGTGG TTATATCCTT CTAGAAGAGG CTCGAAAAAA AACCTTTTTA ATTCCGCTCA AAGCTCTCAT TAAGAATAAG GAAGGTAAAA GCGGAACCGT CTTTGTTGCA AACGAAGAAA ATCGGTTGTT TAGCGTGGAG GTGGAAATCA TTAAAGAGGA AAGCGGTTTT GTGGAAGTAT TAGGAAATCT TGAATTAGGA CGATATATTG CGATTTCCGA TTTGAATGAA CTCAAGGAAG GTCAAAAAGT TAAAATTCCA AAGGAACAAA CTCCCAGTTT GATCGAAGAG AAATCTTATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT