LpboA.00010.a
Short chain dehydrogenase
| CENTER ID: | LpboA.00010.a |
| ORGANISM: | Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.00010.a.B1.GE41840 | full length | 1 | 255 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpboA.00010.a.B1.PS38519 | full length | 1 | 255 |
Structures
6UH2
DEPOSITED: 9/26/2019
DETERMINATION: XRay
CLONE: LpboA.00010.a.B1.GE41840
PROTEIN: LpboA.00010.a.B1.PS38519
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_2688 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|355276.3.peg.1525 |
| UniProt: | Q04TM7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILKNFSDEF QDKLVLITGG SGQIGSELVE SYLSVSARVI CLDPEQPSVD YKSNRFEWIQ ADITNRKEIK EIFLSLENQN KIPDILINCA GISVFTPFED RTDEEFNEVV HVNLNGTFLL SQYTFRLWKE KGKKGIILNF GSIYGVSIAD MRIYGDSGRN SPEVYAMTKA GIIHFTKYLA RYAAPYGIRV NCISPGGIFA NQSSDFIQNY IYKTPLGRMG NPSDLVGGVF FLTSSLSEYV TGQNLLIDGG FTIGD
NT Sequence
atgattttga aaaatttctc tgatgaattt caagataaac ttgttttaat tacgggtgga agtgggcaga tagggtcaga actggtagag tcatatttga gcgtatctgc tcgtgttatc tgtttagatc cagaacagcc ttcagtagat tacaaatcaa atcggttcga atggattcaa gcagacataa cgaatcgtaa agaaataaaa gagatttttt tatccttaga gaatcaaaat aaaatcccgg acatcttaat caattgtgcg gggatctctg tatttacacc ttttgaggat agaacggacg aagaatttaa cgaagtcgtg catgtaaatt tgaacgggac ttttttactc tctcaatata ctttccgtct ttggaaagaa aaagggaaga aaggaatcat tttgaacttc ggatctatct atggtgtttc gattgcagat atgagaatct atggagactc cggaagaaat agtcctgaag tgtatgcaat gacaaaagct gggatcatac attttacgaa atatctggcc agatatgctg ctccttacgg aatcagagtc aattgcatta gccctggagg aatttttgca aaccagagtt ctgattttat tcaaaattat atatataaaa cacctcttgg tagaatgggt aatccgtccg atctcgttgg tggtgttttc tttttaactt cttctctatc ggaatatgta acgggtcaga atcttctcat tgatggaggg tttacgattg gggattga
Details for LpboA.00010.a.B1.GE41840
| HARVESTED ON: | 8/31/2017 |
| SEQUENCED ON: | 9/29/2017 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI LKNFSDEFQD KLVLITGGSG QIGSELVESY LSVSARVICL DPEQPSVDYK SNRFEWIQAD ITNRKEIKEI FLSLENQNKI PDILINCAGI SVFTPFEDRT DEEFNEVVHV NLNGTFLLSQ YTFRLWKEKG KKGIILNFGS IYGVSIADMR IYGDSGRNSP EVYAMTKAGI IHFTKYLARY AAPYGIRVNC ISPGGIFANQ SSDFIQNYIY KTPLGRMGNP SDLVGGVFFL TSSLSEYVTG QNLLXDGGFT IGD
Validated NT Sequence
atcttactca cttaatcccc aatcgtaaac cctccatcnn ngagaagatt ctgacccgtt acatattccg atagagaaga agttaaaaag aaaacaccac caacgagatc ggacggatta cccattctac caagaggtgt tttatatata taattttgaa taaaatcaga actctggttt gcaaaaattc ctccagggct aatgcaattg actctgattc cgtaaggagc agcatatctg gccagatatt tcgtaaaatg tatgatccca gcttttgtca ttgcatacac ttcaggacta tttcttccgg agtctccata gattctcata tctgcaatcg aaacaccata gatagatccg aagttcaaaa tgattccttt cttccctttt tctttccaaa gacggaaagt atattgagag agtaaaaaag tcccgttcaa atttacatgc acgacttcgt taaattcttc gtccgttcta tcctcaaaag gtgtaaatac agagatcccc gcacaattga ttaagatgtc cgggatttta ttttgattct ctaaggataa aaaaatctct tttatttctt tacgattcgt tatgtctgct tgaatccatt cgaaccgatt tgatttgtaa tctactgaag gctgttctgg atctaaacag ataacacgag cagatacgct caaatatgac tctaccagtt ctgaccctat ctgcccactt ccacccgtaa ttaaaacaag tttatcttga aattcatcag agaaattttt caaaatcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMI LKNFSDEFQD KLVLITGGSG QIGSELVESY LSVSARVICL DPEQPSVDYK SNRFEWIQAD ITNRKEIKEI FLSLENQNKI PDILINCAGI SVFTPFEDRT DEEFNEVVHV NLNGTFLLSQ YTFRLWKEKG KKGIILNFGS IYGVSIADMR IYGDSGRNSP EVYAMTKAGI IHFTKYLARY AAPYGIRVNC ISPGGIFANQ SSDFIQNYIY KTPLGRMGNP SDLVGGVFFL TSSLSEYVTG QNLLIDGGFT IGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattt tgaaaaattt ctctgatgaa tttcaagata aacttgtttt aattacgggt ggaagtgggc agatagggtc agaactggta gagtcatatt tgagcgtatc tgctcgtgtt atctgtttag atccagaaca gccttcagta gattacaaat caaatcggtt cgaatggatt caagcagaca taacgaatcg taaagaaata aaagagattt ttttatcctt agagaatcaa aataaaatcc cggacatctt aatcaattgt gcggggatct ctgtatttac accttttgag gatagaacgg acgaagaatt taacgaagtc gtgcatgtaa atttgaacgg gactttttta ctctctcaat atactttccg tctttggaaa gaaaaaggga agaaaggaat cattttgaac ttcggatcta tctatggtgt ttcgattgca gatatgagaa tctatggaga ctccggaaga aatagtcctg aagtgtatgc aatgacaaaa gctgggatca tacattttac gaaatatctg gccagatatg ctgctcctta cggaatcaga gtcaattgca ttagccctgg aggaattttt gcaaaccaga gttctgattt tattcaaaat tatatatata aaacacctct tggtagaatg ggtaatccgt ccgatctcgt tggtggtgtt ttctttttaa cttcttctct atcggaatat gtaacgggtc agaatcttct cattgatgga gggtttacga ttggggattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LpboA.00010.a.B1.PS38519
| PURIFICATION DATe: | 10/16/2018 |
| CONCENTRATION: | 51.06mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI LKNFSDEFQD KLVLITGGSG QIGSELVESY LSVSARVICL DPEQPSVDYK SNRFEWIQAD ITNRKEIKEI FLSLENQNKI PDILINCAGI SVFTPFEDRT DEEFNEVVHV NLNGTFLLSQ YTFRLWKEKG KKGIILNFGS IYGVSIADMR IYGDSGRNSP EVYAMTKAGI IHFTKYLARY AAPYGIRVNC ISPGGIFANQ SSDFIQNYIY KTPLGRMGNP SDLVGGVFFL TSSLSEYVTG QNLLXDGGFT IGD
Validated NT Sequence
atcttactca cttaatcccc aatcgtaaac cctccatcnn ngagaagatt ctgacccgtt acatattccg atagagaaga agttaaaaag aaaacaccac caacgagatc ggacggatta cccattctac caagaggtgt tttatatata taattttgaa taaaatcaga actctggttt gcaaaaattc ctccagggct aatgcaattg actctgattc cgtaaggagc agcatatctg gccagatatt tcgtaaaatg tatgatccca gcttttgtca ttgcatacac ttcaggacta tttcttccgg agtctccata gattctcata tctgcaatcg aaacaccata gatagatccg aagttcaaaa tgattccttt cttccctttt tctttccaaa gacggaaagt atattgagag agtaaaaaag tcccgttcaa atttacatgc acgacttcgt taaattcttc gtccgttcta tcctcaaaag gtgtaaatac agagatcccc gcacaattga ttaagatgtc cgggatttta ttttgattct ctaaggataa aaaaatctct tttatttctt tacgattcgt tatgtctgct tgaatccatt cgaaccgatt tgatttgtaa tctactgaag gctgttctgg atctaaacag ataacacgag cagatacgct caaatatgac tctaccagtt ctgaccctat ctgcccactt ccacccgtaa ttaaaacaag tttatcttga aattcatcag agaaattttt caaaatcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMI LKNFSDEFQD KLVLITGGSG QIGSELVESY LSVSARVICL DPEQPSVDYK SNRFEWIQAD ITNRKEIKEI FLSLENQNKI PDILINCAGI SVFTPFEDRT DEEFNEVVHV NLNGTFLLSQ YTFRLWKEKG KKGIILNFGS IYGVSIADMR IYGDSGRNSP EVYAMTKAGI IHFTKYLARY AAPYGIRVNC ISPGGIFANQ SSDFIQNYIY KTPLGRMGNP SDLVGGVFFL TSSLSEYVTG QNLLIDGGFT IGD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTT TGAAAAATTT CTCTGATGAA TTTCAAGATA AACTTGTTTT AATTACGGGT GGAAGTGGGC AGATAGGGTC AGAACTGGTA GAGTCATATT TGAGCGTATC TGCTCGTGTT ATCTGTTTAG ATCCAGAACA GCCTTCAGTA GATTACAAAT CAAATCGGTT CGAATGGATT CAAGCAGACA TAACGAATCG TAAAGAAATA AAAGAGATTT TTTTATCCTT AGAGAATCAA AATAAAATCC CGGACATCTT AATCAATTGT GCGGGGATCT CTGTATTTAC ACCTTTTGAG GATAGAACGG ACGAAGAATT TAACGAAGTC GTGCATGTAA ATTTGAACGG GACTTTTTTA CTCTCTCAAT ATACTTTCCG TCTTTGGAAA GAAAAAGGGA AGAAAGGAAT CATTTTGAAC TTCGGATCTA TCTATGGTGT TTCGATTGCA GATATGAGAA TCTATGGAGA CTCCGGAAGA AATAGTCCTG AAGTGTATGC AATGACAAAA GCTGGGATCA TACATTTTAC GAAATATCTG GCCAGATATG CTGCTCCTTA CGGAATCAGA GTCAATTGCA TTAGCCCTGG AGGAATTTTT GCAAACCAGA GTTCTGATTT TATTCAAAAT TATATATATA AAACACCTCT TGGTAGAATG GGTAATCCGT CCGATCTCGT TGGTGGTGTT TTCTTTTTAA CTTCTTCTCT ATCGGAATAT GTAACGGGTC AGAATCTTCT CATTGATGGA GGGTTTACGA TTGGGGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT