LetaA.20384.a

Protein kinase, putative

CENTER ID: LetaA.20384.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.20384.a.HZ2.GE43739 kinase domain (Jensen) 1 493
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP21.0280

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVDTVKGR YSSYKLLNEV GRGGCAVVYH AQDLATRKDV AVKQLFGKRP QDMEDWLREV DALNTLNSHH CPHVVKYLDH MQQHDRLFLV EEFTEHGSLL RCLKKKGKLS EDVVCRYIHQ VLTALYHMAP WGVVHGDLKA SNILLFDGDV VKLTDFALRS RSENGHEDAE RRVTAASTPS ADTPPPIRGS HAGRGTGEEC AGLALLEFRG SPYWAAPEVL AGSSKATTAS DIWSLGCLAV ELLTGSPPYF ERPVHNAIHH ILKKYYEVLC ESDTIGGGAG GTSRTPPSPA TPSAATGSGD DDGPRGPRKK KNAVSAMRAT ATTSTDNLPK EPVDVARATV SSGTDSALDR NEVGTPLCEA ALLPPLPGDL HVSDECASFL RLCFRPRAVD RRSAGELLHE PWFLDCTVPQ LLRAAREGRP MNGTTSDEGV SSGSRFAMIE QWVKRNLTCA QESRCKAWLN SDALPLLVPV LTPRIMTPKY IGDVMCVFAQ FAESRSALAM LFVNRLGSTD LWGVEELTSA CDADHLATLF QRCCATQDAQ VPLYTPTDPR ALRFVLGLEK EKALACVRAL HSRLVLEPKA TGSLATVTDG NAVDLNNGSA SSREPTAPAH VASDCTPASL QEQHEQQRRA RERLLNDGGA AALCQCVEAQ CKAAFLSNTA PMMEWSTMNL LFDVLSTIEP LAGGKALLWG FGADISGGHV SPSSTVGTGS GGAAVAKSLP VATNPFGGRG GGELNSTWEK SASGTGAPLL SSTLGSQQES LYTTVAAVGV SGGLLSSLGS TMGFGGYTIS GPPPESVQWA TSMSWLLAVQ EASRHLCEAA VRLLTRYVPL ARRCRAEYLE KAGVSLTATL VLVASSERVS TELRCAAVES LPQLQASSLR AARYLRDPVR CIALLALTLK RTYGVPALTS CILTAITAMT AEKQMLAACT GCPCVWEALV SLLREVEAAD HAVAADGKLS RSHSHSATGT DSQAVEGADA SPALTAAVAS PTSAQSPSAS SSNDSGTSSA AATFADIIVL LSRWFTEVTP TSLLNNAVTT AATASEAMHA LCHPAAPPPL PVLLQTLRRQ LIALSQNGRV CHDDLMPDVA KALRHLAPLE AAAVAASTPT ATVA
NT Sequence
ATGGCCACCG TAGACACCGT CAAAGGGCGG TATTCGTCCT ACAAGCTGCT CAACGAAGTT GGACGTGGCG GCTGTGCGGT CGTGTACCAC GCGCAAGACT TGGCCACGCG TAAGGATGTC GCTGTAAAGC AGCTCTTCGG CAAGCGGCCG CAGGATATGG AAGACTGGTT GCGCGAGGTG GATGCCCTCA ACACACTCAA CAGCCATCAT TGCCCGCACG TGGTGAAGTA CCTGGACCAC ATGCAGCAGC ACGATCGGCT CTTCCTCGTC GAGGAATTCA CCGAGCACGG ATCGCTGCTG CGGTGTCTGA AGAAAAAAGG GAAGCTTTCA GAGGACGTCG TGTGCCGGTA TATTCACCAG GTGCTGACGG CCCTGTACCA CATGGCCCCA TGGGGCGTCG TGCACGGCGA CTTGAAGGCG TCCAATATTC TTCTGTTCGA CGGCGATGTC GTGAAACTCA CAGACTTTGC GCTGCGTTCG CGTAGCGAGA ACGGCCACGA AGATGCCGAG CGGCGGGTCA CCGCGGCCTC CACGCCTTCT GCCGACACAC CCCCTCCCAT TAGGGGCAGC CACGCTGGGA GGGGCACTGG TGAAGAGTGC GCGGGCTTAG CCCTCTTGGA GTTTCGTGGC TCTCCCTACT GGGCAGCGCC GGAAGTCCTC GCAGGCTCCT CGAAGGCAAC GACGGCGAGC GACATCTGGT CGTTGGGCTG CCTCGCGGTG GAGCTTCTCA CGGGCTCCCC GCCATACTTC GAGCGTCCTG TTCACAACGC CATTCATCAC ATCCTCAAGA AGTACTACGA GGTACTGTGT GAAAGCGACA CCATCGGCGG AGGTGCTGGC GGCACCTCGC GCACGCCCCC CAGCCCCGCA ACCCCAAGCG CGGCAACAGG CTCCGGCGAC GATGACGGGC CGCGTGGGCC CCGGAAGAAG AAAAATGCAG TGTCCGCCAT GAGGGCAACG GCAACGACGA GCACCGACAA CCTGCCCAAG GAGCCGGTGG ATGTGGCCCG GGCAACCGTG TCTAGTGGCA CCGACAGTGC ATTAGATCGG AATGAGGTGG GCACACCTCT GTGCGAGGCG GCGCTCCTCC CGCCGCTTCC AGGAGACCTG CACGTGAGCG ATGAGTGTGC GTCGTTTCTA CGGCTGTGCT TCCGCCCCCG CGCGGTGGAC CGACGGTCGG CGGGGGAGCT GCTCCACGAA CCGTGGTTCC TCGACTGCAC GGTGCCGCAG CTCCTGCGGG CAGCGCGCGA GGGTCGGCCG ATGAACGGGA CAACAAGCGA CGAGGGCGTC AGCAGTGGCA GCCGCTTTGC GATGATTGAG CAGTGGGTGA AGCGTAACCT GACATGTGCG CAAGAGAGCC GGTGCAAGGC GTGGCTGAAC AGCGACGCAC TGCCGCTGCT GGTGCCGGTG CTGACGCCGC GCATTATGAC GCCCAAGTAC ATCGGAGATG TAATGTGCGT CTTTGCCCAA TTTGCCGAAA GCCGCTCAGC CTTGGCGATG CTCTTTGTGA ATCGGCTGGG GTCGACAGAC CTCTGGGGAG TGGAGGAGCT GACGTCGGCG TGCGACGCGG ACCACCTCGC CACACTCTTT CAGCGGTGCT GTGCAACGCA GGACGCACAG GTGCCCTTGT ACACCCCGAC AGACCCGCGT GCGCTGCGCT TTGTGTTAGG CCTGGAAAAG GAAAAGGCTC TGGCATGTGT ACGTGCGTTG CACTCGCGTC TCGTACTGGA GCCGAAGGCC ACGGGCTCGT TGGCCACCGT CACAGACGGC AACGCAGTGG ATCTGAACAA CGGCTCTGCC TCCTCGCGTG AGCCCACGGC ACCCGCGCAT GTGGCATCGG ATTGCACGCC CGCATCCCTC CAAGAGCAGC ATGAGCAGCA GCGACGTGCC CGTGAGCGGC TACTCAACGA CGGCGGCGCG GCGGCGCTGT GCCAGTGCGT GGAGGCACAG TGCAAAGCCG CTTTCCTGAG CAACACAGCG CCCATGATGG AGTGGAGCAC AATGAACCTG CTGTTCGACG TGCTCTCCAC CATCGAGCCG CTGGCGGGTG GAAAGGCGCT GCTGTGGGGG TTCGGCGCTG ACATCAGCGG TGGCCACGTC TCCCCAAGTT CTACCGTGGG CACAGGTAGT GGTGGTGCTG CTGTTGCGAA ATCGCTGCCC GTTGCCACCA ACCCGTTCGG AGGACGAGGC GGCGGAGAGT TGAACAGCAC CTGGGAGAAG TCCGCATCAG GGACGGGTGC CCCTCTGTTG TCATCAACGT TGGGGAGCCA GCAGGAATCG CTCTACACGA CAGTGGCAGC GGTGGGTGTC AGCGGCGGCC TGCTGAGCTC CCTAGGCAGC ACCATGGGCT TCGGGGGCTA CACCATCAGT GGGCCCCCAC CGGAGTCAGT GCAGTGGGCC ACATCCATGT CATGGCTGCT CGCAGTGCAG GAAGCGTCGC GCCACCTATG CGAGGCAGCG GTGCGCCTGC TTACTCGCTA TGTTCCACTG GCCAGAAGGT GTAGAGCCGA GTACCTCGAG AAGGCCGGTG TCAGTCTGAC TGCAACGCTA GTCCTCGTGG CCTCAAGCGA GAGGGTCAGC ACCGAGCTGC GATGTGCAGC TGTTGAAAGC TTGCCGCAGC TGCAGGCGAG CTCTCTCCGC GCGGCCCGAT ACCTGCGCGA CCCGGTACGC TGCATTGCCC TGTTGGCGCT CACCCTGAAG CGGACTTATG GTGTCCCTGC GCTGACCTCG TGCATTCTAA CAGCCATCAC CGCTATGACG GCTGAGAAGC AGATGCTAGC TGCCTGCACG GGCTGCCCGT GCGTGTGGGA GGCACTGGTG TCACTGCTAA GGGAAGTGGA GGCAGCTGAC CATGCCGTCG CCGCTGATGG CAAACTGAGC CGCTCGCACT CTCACAGCGC CACCGGCACC GACAGTCAGG CGGTCGAGGG GGCTGACGCT TCTCCTGCTC TAACAGCAGC AGTGGCGAGT CCCACATCAG CTCAATCGCC GAGCGCCAGC AGCAGCAACG ACAGTGGCAC CAGTAGCGCC GCTGCTACCT TTGCCGACAT TATTGTTCTC CTTTCCCGTT GGTTCACGGA GGTGACGCCG ACGTCGCTGC TGAACAATGC AGTGACAACA GCAGCTACTG CATCGGAAGC GATGCATGCC CTCTGCCATC CAGCAGCGCC CCCACCCCTC CCGGTGTTGC TGCAGACGCT TCGCCGCCAG CTCATCGCGC TGTCGCAGAA CGGTCGCGTC TGCCACGATG ATCTGATGCC AGATGTGGCC AAGGCGCTGC GGCACTTGGC CCCTCTCGAA GCCGCCGCGG TCGCTGCGTC AACACCGACG GCCACGGTGG CGTGA
Details for LetaA.20384.a.HZ2.GE43739
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 28
PERCENT COVERAGE: 35
Validated AA Sequence
MYLGVIMRGV STGTSSGSAS LFSHALHRLS CAHVRLRFTH CSIIAKRLPL LTPSSLVVPF IGRPSRAARR SCGTVQSRNH GSWSSSPADR RSTARGRKHS RRNDAHSSLT CRSPGSGGRS AASHRGVPTS FRSNALSVPL DTVARATSTG SLGRLSVLVV AVALMADTAF FFFRGPRGPS SSPEPVAALG VAGLGGVREV PPAPPPMVSL SHSTS
Validated NT Sequence
tttnnntttc nngnntttnn ttagcanncg gatcctcgag aagcttggct gcagaacttg ttcgtgctgt ttattattcg gcaaattggg caaagacgca cattacatct ccgatgtact tgggcgtcat aatgcgcggc gtcagcaccg gcaccagcag cggcagtgcg tcgctgttca gccacgcctt gcaccggctc tcttgcgcac atgtcaggtt acgcttcacc cactgctcaa tcatcgcaaa gcggctgcca ctgctgacac cctcgtcgct tgttgtcccg ttcatcggcc gaccctcgcg cgctgcccgc aggagctgcg gcaccgtgca gtcgaggaac cacggttcgt ggagcagctc ccccgccgac cgtcggtcca ccgcgcgggg gcggaagcac agccgtagaa acgacgcaca ctcatcgctc acgtgcaggt ctcctggaag cggcgggagg agcgccgcct cgcacagagg tgtgcccacc tcattccgat ctaatgcact gtcggtgcca ctagacacgg ttgcccgggc cacatccacc ggctccttgg gcaggttgtc ggtgctcgtc gttgccgttg ccctcatggc ggacactgca tttttcttct tccggggccc acgcggcccg tcatcgtcgc cggagcctgt tgccgcgctt ggggttgcgg ggctgggggg cgtgcgcgag gtgccgccag cacctccgcc gatggtgtcg ctttcacaca gtacctcgta gtacttcttg aggatgtgat gatggcgttg tgaacaggac gctcgaagta tggcggggan cccgtgagaa gctccaccgc gangcagccc aacgaccaga tgtcgctcgn ccgncnttgc cttcgaggag cctgcgagga cttccggcgc tgnccnnnag ggagagccac gaaactccaa gagggctaag cccgcgcact cttcaccant gccccctccc agcgtgnntg cccctaangg gagggggtnt gtcggnnnaa nncgtggaag nccgcc
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA TVDTVKGRYS SYKLLNEVGR GGCAVVYHAQ DLATRKDVAV KQLFGKRPQD MEDWLREVDA LNTLNSHHCP HVVKYLDHMQ QHDRLFLVEE FTEHGSLLRC LKKKGKLSED VVCRYIHQVL TALYHMAPWG VVHGDLKASN ILLFDGDVVK LTDFALRSRS ENGHEDAERR VTAASTPSAD TPPPIRGSHA GRGTGEECAG LALLEFRGSP YWAAPEVLAG SSKATTASDI WSLGCLAVEL LTGSPPYFER PVHNAIHHIL KKYYEVLCES DTIGGGAGGT SRTPPSPATP SAATGSGDDD GPRGPRKKKN AVSAMRATAT TSTDNLPKEP VDVARATVSS GTDSALDRNE VGTPLCEAAL LPPLPGDLHV SDECASFLRL CFRPRAVDRR SAGELLHEPW FLDCTVPQLL RAAREGRPMN GTTSDEGVSS GSRFAMIEQW VKRNLTCAQE SRCKAWLNSD ALPLLVPVLT PRIMTPKYIG DVMCVFAQFA E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc aaagggcggt attcgtccta caagctgctc aacgaagttg gacgtggcgg ctgtgcggtc gtgtaccacg cgcaagactt ggccacgcgt aaggatgtcg ctgtaaagca gctcttcggc aagcggccgc aggatatgga agactggttg cgcgaggtgg atgccctcaa cacactcaac agccatcatt gcccgcacgt ggtgaagtac ctggaccaca tgcagcagca cgatcggctc ttcctcgtcg aggaattcac cgagcacgga tcgctgctgc ggtgtctgaa gaaaaaaggg aagctttcag aggacgtcgt gtgccggtat attcaccagg tgctgacggc cctgtaccac atggccccat ggggcgtcgt gcacggcgac ttgaaggcgt ccaatattct tctgttcgac ggcgatgtcg tgaaactcac agactttgcg ctgcgttcgc gtagcgagaa cggccacgaa gatgccgagc ggcgggtcac cgcggcctcc acgccttctg ccgacacacc ccctcccatt aggggcagcc acgctgggag gggcactggt gaagagtgcg cgggcttagc cctcttggag tttcgtggct ctccctactg ggcagcgccg gaagtcctcg caggctcctc gaaggcaacg acggcgagcg acatctggtc gttgggctgc ctcgcggtgg agcttctcac gggctccccg ccatacttcg agcgtcctgt tcacaacgcc attcatcaca tcctcaagaa gtactacgag gtactgtgtg aaagcgacac catcggcgga ggtgctggcg gcacctcgcg cacgcccccc agccccgcaa ccccaagcgc ggcaacaggc tccggcgacg atgacgggcc gcgtgggccc cggaagaaga aaaatgcagt gtccgccatg agggcaacgg caacgacgag caccgacaac ctgcccaagg agccggtgga tgtggcccgg gcaaccgtgt ctagtggcac cgacagtgca ttagatcgga atgaggtggg cacacctctg tgcgaggcgg cgctcctccc gccgcttcca ggagacctgc acgtgagcga tgagtgtgcg tcgtttctac ggctgtgctt ccgcccccgc gcggtggacc gacggtcggc gggggagctg ctccacgaac cgtggttcct cgactgcacg gtgccgcagc tcctgcgggc agcgcgcgag ggtcggccga tgaacgggac aacaagcgac gagggcgtca gcagtggcag ccgctttgcg atgattgagc agtgggtgaa gcgtaacctg acatgtgcgc aagagagccg gtgcaaggcg tggctgaaca gcgacgcact gccgctgctg gtgccggtgc tgacgccgcg cattatgacg cccaagtaca tcggagatgt aatgtgcgtc tttgcccaat ttgccgaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a