LetaA.20382.a

Serine/threonine-protein kinase, putative

CENTER ID: LetaA.20382.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.20382.a.HZ2.GE43737 kinase domain (Jensen) 128 574
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP30.3620

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKSKKHGKP VKGASKKGSK NEKSKRKTKF IDPTSSVSRD VRETVAALVE EIEREEPVSE EKLQPLNKEK FQEIAEKEGI LVKTGMTKEQ AHTIANKLKH QQSVTHHSDG EGDVGSESSD GTGDGEGDGG GTEEDYSDTA NEPSREYRKG GYHHVVIGEV YNDRYRIVKK LGWGYFSTVW LVWDYQKERY QAMKIQKSAA SYSEAAYDEI KLLSQIMEAD PHKNRCCARL NDYFKHTGPN GTHVCMLFDV YGENLLSLME RYEYRGIPLP IVKCIARQVL IGLDHINSID IIHTDLKPEN VLLSTPKHSI ISLMKHFHSP PLHQRPKLTE RDPKTMTKSQ RRRYYKKLAK EERKPLLDGN NDDHSCRGED IGSNENGDAD SEGSKTDPEW EVERFHHVIL ADFGNSCWTY KQFTDEVQTR QYRCPEVILG EPYSTPIDIW SCACMIFELI TGQFLFDPKK GDDYSRDEDH LALMSELLGD LPESMRLGDG KYRSYYYNSK GDLRNIKDLQ YWALEDVLHQ RHKFTKKKAK EIADFLLPML EYEPGARATP ATMLRDHDAF FDIQDDDYAP LCFVEDSDDE EGSASDDNET ASDGSSESYT SASSSELKVN RSRQLDNDAD RSETFRYWEE HPILNRTSLE ERGLTIADIQ SVLAGNFLDD ASAHEAAAEV IRLLSEETDR LSNDHGSDGH DADNEDGESS DDEEVITQYS DGPEVVACNA TPCDDGVDEG GEDAAGGEDE GSREVAE
NT Sequence
ATGGGGAAGT CGAAGAAGCA CGGGAAACCC GTAAAAGGCG CCTCGAAGAA GGGGAGCAAG AATGAAAAGA GCAAGCGCAA GACAAAGTTC ATAGACCCCA CGAGCAGCGT AAGCCGCGAT GTTCGTGAAA CCGTGGCGGC CCTCGTCGAG GAAATCGAAC GCGAGGAGCC GGTGAGCGAA GAGAAACTGC AGCCTCTTAA CAAAGAAAAG TTCCAGGAGA TCGCAGAAAA GGAGGGCATC CTCGTCAAAA CCGGCATGAC GAAGGAGCAG GCCCACACGA TCGCCAACAA ACTCAAACAT CAACAGAGCG TAACGCACCA TAGCGACGGC GAGGGCGACG TGGGGAGTGA AAGCAGCGAC GGCACCGGCG ACGGCGAGGG CGACGGTGGC GGAACCGAGG AAGACTACAG TGACACGGCC AATGAGCCTT CCCGGGAGTA CCGCAAGGGA GGCTACCACC ACGTTGTCAT TGGTGAAGTC TATAACGACC GCTACCGCAT CGTCAAGAAG CTGGGATGGG GCTATTTCTC TACCGTGTGG CTAGTGTGGG ATTATCAGAA GGAGCGCTAC CAGGCAATGA AGATTCAGAA ATCTGCGGCG AGCTATAGCG AGGCTGCCTA CGACGAGATC AAGCTGCTCT CTCAGATTAT GGAGGCAGAT CCACACAAGA ATCGTTGTTG TGCCCGCTTG AATGACTACT TCAAGCACAC GGGACCGAAC GGTACACACG TTTGCATGCT TTTTGATGTG TACGGCGAGA ACCTGCTCTC CCTGATGGAG CGCTACGAGT ATCGCGGCAT CCCGCTACCG ATTGTGAAGT GCATTGCGCG GCAAGTGCTG ATAGGCCTCG ATCACATCAA CTCCATCGAC ATCATCCACA CAGACCTTAA ACCGGAGAAT GTGCTCTTAT CGACGCCGAA GCATTCTATA ATATCACTGA TGAAGCATTT TCATTCGCCG CCGCTGCACC AACGCCCAAA ACTAACGGAA CGCGACCCGA AGACCATGAC CAAATCACAG CGAAGACGGT ACTACAAGAA GTTGGCGAAG GAGGAGCGAA AGCCGCTCCT GGACGGGAAC AATGACGACC ACAGCTGTAG AGGTGAAGAC ATCGGCAGTA ACGAGAACGG GGACGCCGAC TCAGAAGGAA GCAAGACAGA TCCGGAGTGG GAAGTGGAGC GCTTCCACCA TGTCATTCTC GCCGACTTTG GGAATAGCTG CTGGACCTAC AAACAGTTTA CGGACGAGGT GCAGACACGG CAGTATCGCT GCCCTGAAGT TATTCTAGGC GAGCCATATT CTACGCCAAT CGATATTTGG TCTTGCGCCT GCATGATTTT TGAGCTCATC ACTGGCCAGT TTCTCTTCGA TCCCAAGAAA GGAGACGACT ACTCGCGTGA TGAGGATCAT CTAGCTCTCA TGAGCGAACT GCTCGGCGAC CTGCCGGAGA GTATGCGACT GGGTGACGGC AAGTACCGAA GCTACTACTA CAACTCAAAA GGAGACTTGC GCAACATCAA GGATCTACAG TACTGGGCCC TAGAAGACGT ACTGCATCAG CGGCATAAGT TTACTAAGAA AAAGGCTAAA GAAATTGCCG ACTTCTTGCT TCCCATGCTA GAGTACGAGC CAGGCGCCCG CGCTACCCCT GCGACAATGC TGCGTGACCA CGACGCATTC TTTGACATCC AAGACGACGA CTACGCGCCG CTGTGTTTTG TTGAGGACAG CGACGATGAG GAAGGGTCTG CGTCGGATGA TAATGAGACT GCCAGCGATG GGTCTTCGGA GTCCTACACC TCTGCTTCTT CCAGCGAGCT AAAGGTCAAT CGGTCCCGCC AGCTTGACAA TGATGCCGAC CGCTCCGAGA CCTTTCGGTA TTGGGAGGAG CATCCAATTC TGAACCGTAC TTCCCTAGAG GAGCGTGGGC TGACTATTGC TGATATTCAG TCGGTTCTCG CCGGGAACTT CTTAGATGAC GCCTCAGCCC ATGAGGCTGC CGCTGAGGTG ATTCGTCTGC TCTCAGAGGA GACGGACCGC CTTTCGAACG ACCATGGTAG CGATGGACAC GACGCGGACA ATGAGGACGG GGAAAGCAGC GATGATGAAG AGGTGATCAC GCAGTACTCT GACGGGCCAG AGGTGGTGGC GTGTAATGCG ACTCCGTGCG ACGATGGCGT CGACGAAGGC GGCGAGGATG CCGCTGGTGG AGAGGATGAG GGCAGTAGAG AGGTCGCTGA GTAG
Details for LetaA.20382.a.HZ2.GE43737
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSDG GGTEEDYSDT ANEPSREYRK GGYHHVVIGE VYNDRYRIVK KLGWGYFSTV WLVWDYQKER YQAMKIQKSA ASYSEAAYDE IKLLSQIMEA DPHKNRCCAR LNDYFKHTGP NGTHVCMLFD VYGENLLSLM ERYEYRGIPL PIVKCIARQV LIGLDHINSI DIIHTDLKPE NVLLSTPKHS IISLMKHFHS PPLHQRPKLT ERDPKTMTKS QRRRYYKKLA KEERKPLLDG NNDDHSCRGE DIGSNENGDA DSEGSKTDPE WEVERFHHVI LADFGNSCWT YKQFTDEVQT RQYRCPEVIL GEPYSTPIDI WSCACMIFEL ITGQFLFDPK KGDDYSRDED HLALMSELLG DLPESMRLGD GKYRSYYYNS KGDLRNIKDL QYWALEDVLH QRHKFTKKKA KEIADFLLPM LEYEPGARAT PATMLRDHDA FFDIQDDDYA PLCFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg acggtggcgg aaccgaggaa gactacagtg acacggccaa tgagccttcc cgggagtacc gcaagggagg ctaccaccac gttgtcattg gtgaagtcta taacgaccgc taccgcatcg tcaagaagct gggatggggc tatttctcta ccgtgtggct agtgtgggat tatcagaagg agcgctacca ggcaatgaag attcagaaat ctgcggcgag ctatagcgag gctgcctacg acgagatcaa gctgctctct cagattatgg aggcagatcc acacaagaat cgttgttgtg cccgcttgaa tgactacttc aagcacacgg gaccgaacgg tacacacgtt tgcatgcttt ttgatgtgta cggcgagaac ctgctctccc tgatggagcg ctacgagtat cgcggcatcc cgctaccgat tgtgaagtgc attgcgcggc aagtgctgat aggcctcgat cacatcaact ccatcgacat catccacaca gaccttaaac cggagaatgt gctcttatcg acgccgaagc attctataat atcactgatg aagcattttc attcgccgcc gctgcaccaa cgcccaaaac taacggaacg cgacccgaag accatgacca aatcacagcg aagacggtac tacaagaagt tggcgaagga ggagcgaaag ccgctcctgg acgggaacaa tgacgaccac agctgtagag gtgaagacat cggcagtaac gagaacgggg acgccgactc agaaggaagc aagacagatc cggagtggga agtggagcgc ttccaccatg tcattctcgc cgactttggg aatagctgct ggacctacaa acagtttacg gacgaggtgc agacacggca gtatcgctgc cctgaagtta ttctaggcga gccatattct acgccaatcg atatttggtc ttgcgcctgc atgatttttg agctcatcac tggccagttt ctcttcgatc ccaagaaagg agacgactac tcgcgtgatg aggatcatct agctctcatg agcgaactgc tcggcgacct gccggagagt atgcgactgg gtgacggcaa gtaccgaagc tactactaca actcaaaagg agacttgcgc aacatcaagg atctacagta ctgggcccta gaagacgtac tgcatcagcg gcataagttt actaagaaaa aggctaaaga aattgccgac ttcttgcttc ccatgctaga gtacgagcca ggcgcccgcg ctacccctgc gacaatgctg cgtgaccacg acgcattctt tgacatccaa gacgacgact acgcgccgct gtgttttgtt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa