LetaA.20227.a

Protein kinase, putative (OPH8)

CENTER ID: LetaA.20227.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.20227.a.B2.GE42829 PHoranyi kinase domain truncation 438 741
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP25.1590

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSSKRHAS EPLPQQQQQQ QHSTDPPSAY QHSRVVTEAG LDNASLPQLP PNLTPPVTNI ETPSNPAPLF TSAASSTLVP IFQRRTGSVE SSRGDGDDNA EVHRHARVSQ SYPGASAKAV QSMALAVVPF TGTSGCGVGS DGAGQEGKNM GGSGNGVGYY MGPAPYGKLA PLSLSAERVC PHCLRPYDVF DDAEDVFNPE AFFRVDATTR PSSMQPSPSH PKLLPPPLQE GDEQEKQQPS TSPRLYTDGA ADTGSAAGPL LCSRTSTTAG LDDNSSDGIN RSGNYITALH PPAAAFTSHY FRALPPPSFI VAAGAHGASA PLAIEDYRPS PLLDSPRNEN EEGGQRFTVA PGPTSDRGDV GGDPSGALVC KVASVPHYSY VWPITCTGMR SQVRLLQQDD GNEKTVAEAE GEKQESLSCG SGSPVCSPIA STPSPNNGYY RHYFKELRQL GRGTYGGVYL CRHIMCGVNL GEFALKKIPV GDKVTYLQSV LREVRILEEV RRHPNVVEYK HSWVEEAQLA DFGPPVRCLF ILMEYASAGS LDTYLERYGN NLSTLAVWYF FLSSVAGTAH LHEKHILHRD LKPQNLLLAA TKGRPPRVLV SDFGTAALLD DISYDRSGGT GTLEYMAPEL LETIASPRGT NEQYVNHHTM ASDVWSLGMV LHYLAFDGTL PERRKDGSVN LDKARHLDNT RPPEMLRLLE AMLQLDPAKR PRCSDILGSP MVQSIMRIFN KDDHSQWDLS IQRQQKLSAT SPSSILPLER PSRPPAPASA PKTSSSSAPR GALSRNVVAA VSTSHGRGVR ADRTNVEDNS NVLHSSRVEL LSASTLVDLS TVMARGGSGS SSNGSPLAMK LPPPRPPSTV IPQRAQQQRQ RTHIVDVLPQ ALASFNSQLL DSPSRRRINV GVQTNPVVIV DKQQQHTEGE EERVVQ
NT Sequence
atgaacccgt cgtcaaagcg gcacgcgtcc gagccgctgc cgcagcagca gcagcagcag cagcattcca cagacccgcc gtccgcctac cagcatagcc gagtagtcac agaggccggt ctcgacaatg cttcccttcc acagctccca ccgaacttga cgcccccggt gaccaacatt gagacccctt caaaccccgc ccccctcttc acctcggcag cgtcgtcgac cctggtaccc atcttccaaa ggcggacggg ctctgtagaa tcttcacgag gtgacggcga cgacaatgcg gaagtgcatc gtcacgcacg agtaagccaa agctaccctg gggcgtctgc caaggcagtg cagtccatgg cgctagcggt ggtgcccttc acaggcacta gtggctgtgg agtgggcagt gacggtgccg gccaggaagg aaagaacatg ggtggtagtg gtaatggcgt tggctactac atggggcctg ccccgtatgg caagctggca ccgttgtcgc taagcgcaga gcgagtgtgt ccccactgcc tgcgaccgta cgacgtcttc gacgacgccg aagacgtctt caacccggag gccttcttcc gcgtggatgc gacaacgagg ccatcctcga tgcagccttc accaagtcat ccgaagcttc ttccaccgcc gctgcaggag ggtgatgagc aggagaagca gcagccatcg acgtctccgc gcctatacac tgacggcgca gcggatactg gcagcgctgc tgggcccctt ttgtgcagca ggactagcac gaccgctggg ctcgatgaca acagcagtga tggcatcaac cgcagcggca actacatcac ggccttacat ccgccagctg ctgcgttcac ctcgcactac ttccgtgctc tgccaccgcc ctcatttatc gtagcggcgg gagcccacgg cgcatcggca ccgcttgcca tcgaggacta ccgcccatca ccgctactcg atagcccgag gaacgagaat gaggaagggg gacagcggtt cacagtggcc cctgggccta ctagtgaccg tggtgatgtt ggtggggacc cgagtggggc gctggtgtgc aaagtagctt ccgtaccgca ctactcctac gtttggccca tcacttgcac aggcatgcgc tcgcaggtgc gactgctgca gcaggatgac ggcaacgaga aaactgtagc agaggcggag ggcgagaagc aggagtcgct ctcgtgtggg agtggctctc ccgtctgctc ccccatcgcg tcgacgccgt cgccgaacaa tggctactac cgtcattact tcaaggaact ccgtcagctg ggccgtggca cctatggtgg tgtctacctc tgtcgtcaca ttatgtgcgg cgttaacctt ggcgagttcg ctctgaagaa gattccagtc ggcgacaagg tgacctatct gcaatcggtg ttgcgcgagg tacgcattct cgaggaggtg cggcgacacc ccaatgtggt cgagtacaag cacagctggg tggaggaggc gcagctcgcc gactttggcc cgcctgtgcg ctgcctcttc atcttgatgg agtatgcgtc cgctggctcg cttgacacgt atctggagcg ctacggcaac aatctttcca ccctggccgt gtggtacttc ttcctcagct ccgtggcagg tacggcgcat cttcacgaga agcacatctt gcaccgcgac ttaaagcctc aaaatctgtt gctagccgcg acgaagggcc gtccaccgcg cgtgcttgtc agtgactttg gtacggcagc gctgctcgat gatatctcgt atgaccggtc cggtggcacg ggcacgctcg agtacatggc gccggagctc ctcgaaacaa tcgcctctcc acgcggaacc aacgaacagt atgtgaatca tcacacgatg gcctctgacg tgtggtcact ggggatggtg ctgcactacc tcgccttcga cgggacgctg ccggagcgac gcaaggacgg tagtgtcaac ctcgacaagg cacgccactt ggacaatacc aggccacctg agatgctcag actgctggaa gccatgctgc agcttgatcc cgcaaagcgg ccgcgctgca gcgacatcct cggctcccca atggtgcagt ctatcatgcg catcttcaac aaggatgacc atagccagtg ggacctctcc atccagcggc agcaaaagct ctcggccacc tcgccgtctt cgattcttcc gttggagcgt ccctctcgcc caccggcacc ggcatcagct cccaagacca gtagcagcag cgcaccccgc ggcgctctca gtagaaacgt tgttgccgcc gtctctacct cacatggacg tggcgtgcgg gccgaccgca caaatgtgga agacaatagc aacgtgcttc acagcagccg tgtcgagctc ctctcagcgt cgacgttggt ggatctgtca acggtgatgg cacgtggcgg gagtggcagc agcagcaatg gtagtcccct tgccatgaag ttgcctcctc cgcgccctcc atccaccgtg attccccagc gtgcccaaca gcagcggcaa cgcacgcata tagtggacgt gttaccgcag gccctagcat ccttcaatag ccagctttta gattctccat cgcgacgcag gatcaacgtc ggcgtgcaaa caaatccggt ggtgatagtg gacaagcagc agcagcatac agaaggggag gaggaacgag tagtacagta g
Details for LetaA.20227.a.B2.GE42829
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG YYRHYFKELR QLGRGTYGGV YLCRHIMCGV NLGEFALKKI PVGDKVTYLQ SVLREVRILE EVRRHPNVVE YKHSWVEEAQ LADFGPPVRC LFILMEYASA GSLDTYLERY GNNLSTLAVW YFFLSSVAGT AHLHEKHILH RDLKPQNLLL AATKGRPPRV LVSDFGTAAL LDDISYDRSG GTGTLEYMAP ELLETIASPR GTNEQYVNHH TMASDVWSLG MVLHYLAFDG TLPERRKDGS VNLDXXRHLD NTRPPEMLRL LEAMXQLDPA KRPXXSDILG SPXVQSIMRI FNK
Validated NT Sequence
ccttgttgaa gatgcgcatg atagactgca ccnttgggga gccgaggatg tcgctgcnnn gcggccgctt tgcgggatca agctgcnnca tggcttccag cagtctgagc atctcaggtg gcctggtatt gtccaagtgg cgtnnnttgt cgaggttgac actaccgtcc ttgcgtcgct ccggcagcgt cccgtcgaag gcgaggtagt gcagcaccat ccccagtgac cacacgtcag aggccatcgt gtgatgattc acatactgtt cgttggttcc gcgtggagag gcgattgttt cgaggagctc cggcgccatg tactcgagcg tgcccgtgcc accggaccgg tcatacgaga tatcatcgag cagcgctgct gtaccaaagt cactgacaag cacgcgcggt ggacggccct tcgtcgcggc tagcaacaga ttttgaggct ttaagtcgcg gtgcaagatg tgcttctcgt gaagatgcgc cgtacctgcc acggagctga ggaagaagta ccacacagcc agggtggaaa gattgttgcc gtagcgctcc agatacgtgt caagcgagcc agcggacgca tactccatca agatgaagag gcagcgcaca ggcgggccaa agtcggcgag ctgcgcctcc tccacccagc tgtgcttgta ctcgaccaca ttggggtgtc gccgcacctc ctcgagaatg cgtacctcgc gcaacaccga ttgcagatag gtcaccttgt cgccgactgg aatcttcttc agagcgaact cgccaaggtt aacgccgcac ataatgtgac gacagaggta gacaccacca taggtgccac ggcccagctg acggagctcc ttgaagtaat gacggtagta gcccatatgg tggtggtggt ggtgagccat gg
Expected Protein Sequence
MAHHHHHHGY YRHYFKELRQ LGRGTYGGVY LCRHIMCGVN LGEFALKKIP VGDKVTYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDTYLERYG NNLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA ATKGRPPRVL VSDFGTAALL DDISYDRSGG TGTLEYMAPE LLETIASPRG TNEQYVNHHT MASDVWSLGM VLHYLAFDGT LPERRKDGSV NLDKARHLDN TRPPEMLRLL EAMLQLDPAK RPRCSDILGS PMVQSIMRIF NKDDHSQWDL SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctact accgtcatta cttcaaggaa ctccgtcagc tgggccgtgg cacctatggt ggtgtctacc tctgtcgtca cattatgtgc ggcgttaacc ttggcgagtt cgctctgaag aagattccag tcggcgacaa ggtgacctat ctgcaatcgg tgttgcgcga ggtacgcatt ctcgaggagg tgcggcgaca ccccaatgtg gtcgagtaca agcacagctg ggtggaggag gcgcagctcg ccgactttgg cccgcctgtg cgctgcctct tcatcttgat ggagtatgcg tccgctggct cgcttgacac gtatctggag cgctacggca acaatctttc caccctggcc gtgtggtact tcttcctcag ctccgtggca ggtacggcgc atcttcacga gaagcacatc ttgcaccgcg acttaaagcc tcaaaatctg ttgctagccg cgacgaaggg ccgtccaccg cgcgtgcttg tcagtgactt tggtacggca gcgctgctcg atgatatctc gtatgaccgg tccggtggca cgggcacgct cgagtacatg gcgccggagc tcctcgaaac aatcgcctct ccacgcggaa ccaacgaaca gtatgtgaat catcacacga tggcctctga cgtgtggtca ctggggatgg tgctgcacta cctcgccttc gacgggacgc tgccggagcg acgcaaggac ggtagtgtca acctcgacaa ggcacgccac ttggacaata ccaggccacc tgagatgctc agactgctgg aagccatgct gcagcttgat cccgcaaagc ggccgcgctg cagcgacatc ctcggctccc caatggtgca gtctatcatg cgcatcttca acaaggatga ccatagccag tgggacctct ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat