LetaA.20202.a

Hypothetical protein, conserved (GO function: tRNA aminoacylation, translation)

CENTER ID: LetaA.20202.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.20202.a.B1.GE42557 full length 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP15.0680

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVFMRVGE LACQKDSFLR ELTATVVSCE PAKTAVATAK KAKGASNDTV AYDVVLSDSV LFPEGGGQPC DYGTLRVVSS DKDKSDPSSE ATTVPLIPIT SVRRVGDTCV LTSPTALHVG ATVHQMVDWP RRLDHMQHHT GQHLLTAIVE RKDTLHLPTV SWSLTHPHCF IQVDVGAYAS DAASPYHTYI TKEKKISDEM VAKVEALCND AITRSTAVHC DVFENRQVYV QEQDRRCQST AASADGGEEA FRSRGIPEDV TGPIRIISLD AIDSCTCCGT HLHTLAELKA MYLLHQEVKG TLVKLYFITG DRAAQEFHAM YQRERQLMLE LGGSRPNDFV SVVQRRSKDA INLEKLLKRW TVELASVEAQ RIAATVSSSN STTVMVPLRR DDVELDFFNE VRARLDAAGL RHVVLVSAWA ADRCTAGPLA SAKDVAGQVF VTGGETPADM EKAVQLVKET LEGMKGGTSK LGFRGKGSLK EWDTLVARLQ E
NT Sequence
ATGGCAGCTG TATTCATGCG CGTTGGTGAA CTAGCGTGTC AGAAGGATTC GTTCCTTCGA GAGTTGACCG CCACTGTTGT CTCGTGTGAG CCGGCCAAGA CGGCAGTGGC AACGGCGAAG AAGGCGAAGG GCGCCAGCAA CGACACTGTG GCGTACGACG TTGTTCTGAG CGACTCGGTC CTTTTTCCAG AGGGCGGTGG TCAGCCCTGC GACTATGGCA CATTACGAGT GGTGTCCAGC GATAAGGACA AGTCGGATCC CTCGAGTGAA GCCACCACCG TGCCCCTGAT CCCCATTACG AGCGTGCGCC GGGTCGGGGA CACATGCGTG CTAACCAGTC CCACCGCCCT CCACGTGGGG GCTACGGTGC ACCAAATGGT AGACTGGCCC CGTCGGCTCG ACCATATGCA GCACCACACC GGCCAGCACC TGCTCACGGC CATTGTAGAG CGCAAGGACA CGCTACACTT GCCTACCGTC AGCTGGTCCC TGACGCACCC CCACTGCTTC ATCCAGGTGG ACGTTGGCGC CTACGCAAGC GACGCCGCCT CGCCATACCA CACGTACATC ACAAAGGAGA AAAAAATTTC AGACGAGATG GTGGCAAAGG TGGAGGCACT CTGCAACGAC GCCATCACAC GCAGCACCGC CGTGCACTGC GACGTGTTCG AAAATCGCCA GGTGTATGTG CAAGAGCAGG ATCGCCGGTG TCAGAGCACT GCAGCCTCGG CAGACGGCGG CGAGGAAGCC TTTCGCAGCC GTGGCATCCC AGAGGACGTG ACCGGACCAA TTCGCATCAT CTCACTAGAC GCGATCGACA GCTGCACCTG CTGCGGCACC CACCTTCACA CACTGGCAGA GCTGAAGGCC ATGTACCTCC TCCACCAAGA GGTGAAAGGG ACCCTAGTGA AACTGTACTT CATCACTGGC GACCGGGCCG CACAGGAATT CCACGCCATG TATCAGCGAG AGCGGCAGTT GATGCTGGAG TTGGGTGGCA GCAGACCCAA CGACTTTGTC TCCGTGGTGC AGCGCCGTTC CAAAGATGCG ATTAACCTTG AGAAGCTGCT GAAGAGGTGG ACGGTGGAGC TTGCGAGTGT AGAAGCGCAG CGCATTGCCG CCACTGTGTC ATCGTCAAAC TCCACTACCG TCATGGTACC CCTTCGCCGC GACGACGTAG AGCTCGATTT CTTCAACGAG GTCCGCGCAC GGCTCGACGC GGCAGGGCTT CGCCACGTCG TTCTCGTTTC CGCGTGGGCA GCGGACCGGT GTACAGCAGG CCCGCTCGCC AGCGCCAAGG ATGTGGCGGG TCAGGTCTTC GTCACCGGCG GGGAGACACC TGCGGACATG GAGAAGGCTG TCCAGCTGGT GAAGGAAACG CTAGAGGGGA TGAAGGGAGG CACCTCCAAG CTTGGTTTCC GTGGGAAGGG CAGTCTGAAG GAGTGGGACA CCCTCGTGGC ACGTCTCCAG GAGTAG
Details for LetaA.20202.a.B1.GE42557
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 73
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMA AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVATAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDY GTLRVVSSDK DKSDPSSEAT TVPLIPITSV RRVGDTCVLT SPTALHVGAT VHQMVDWPRR LDHMQHHTGQ HLLTAIVERK DTLHLPTVSW SLTHPHCFIQ VDVGAYASDA ASPYHTYITK EKKISDEMVA KVEALCNDAI TRSTAVHCDV XXKSPGVCAR AGSPVSEHCS LGRRRRGXXS QPWHPRGRDR TNSHHLTRRD RQLHLLRHPP SHTGRAEGHV PPPPRGERDP SETVLHHWRP GRTGIPRHVS ARAAVDAGVG WQQTQRLCLR GAAPFQRCD
Validated NT Sequence
ccatggctca ccaccaccac caccatatgg cagctgtatt catgcgcgtt ggtgaactag cgtgtcagaa ggattcgttc cttcgagagt tgaccgccac tgttgtctcg tgtgagccgg ccaagacggc agtggcaacg gcgaagaagg cgaagggcgc cagcaacgac actgtggcgt acgacgttgt tctgagcgac tcggtccttt ttccagaggg cggtggtcag ccctgcgact atggcacatt acgagtggtg tccagcgata aggacaagtc ggatccctcg agtgaagcca ccaccgtgcc cctgatcccc attacgagcg tgcgccgggt cggggacaca tgcgtgctaa ccagtcccac cgccctccac gtgggggcta cggtgcacca aatggtagac tggccccgtc ggctcgacca tatgcagcac cacaccggcc agcacctgct cacggccatt gtagagcgca aggacacgct acacttgcct accgtcagct ggtccctgac gcacccccac tgcttcatcc aggtggacgt tggcgcctac gcaagcgacg ccgcctcgcc ataccacacg tacatcacaa aggagaaaaa aatttcagac gagatggtgg caaaggtgga ggcactctgc aacgacgcca tcacacgcag caccgccgtg cactgcgacg tgnnnngaaa atcnccaggt gtatgtgcaa gagcaggatc gccggtgtca gagcactgca gcctcggcag acggcggcga ggaagnnttt cgcagccgtg gcatcccaga ggacgtgacc ggaccaattc gcatcatctc actagacgcg atcgacagct gcacctgctg cggcacccac cttcacacac tggcagagct gaaggccatg tacctcctcc accaagaggt gaaagggacc ctagtgaaac tgtacttcat cactggcgac cgggccgcac aggaattcca cgccatgtat cagcgagagc ggcagttgat gctggagttg ggtggcagca gacccaacga ctttgtctcc gtggtgcagc gccgttccaa agatgcgatt aaccttgaga agctgctgaa gaggtggacg gtggagcttg cgagtgtaga agcgcagcgc attgccgcca ctgtgtcatc gtcaaactcc actaccgtca tggtacccct tcgccgcgac gacgtagagc tcgatttctt caacaaggtc cgcgcacggc tcgacgcggc agggcttcgc cacgtcgttc tcgtttccgc gtgggcagcg gaccggtgta cagcaggccc gctcgccagc gccaaggatg tggcgggtca ggtcttcgtc accgacgggg agacacctgc ggacatggag aaggctgtcc agctggtgaa ggaaacgcta gaggggatga agggaggcac ctccaagctt ggtttccgtg ggaagggcag tctgaaggag tgggacac
Expected Protein Sequence
MAHHHHHHMA AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVATAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDY GTLRVVSSDK DKSDPSSEAT TVPLIPITSV RRVGDTCVLT SPTALHVGAT VHQMVDWPRR LDHMQHHTGQ HLLTAIVERK DTLHLPTVSW SLTHPHCFIQ VDVGAYASDA ASPYHTYITK EKKISDEMVA KVEALCNDAI TRSTAVHCDV FENRQVYVQE QDRRCQSTAA SADGGEEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL HTLAELKAMY LLHQEVKGTL VKLYFITGDR AAQEFHAMYQ RERQLMLELG GSRPNDFVSV VQRRSKDAIN LEKLLKRWTV ELASVEAQRI AATVSSSNST TVMVPLRRDD VELDFFNEVR ARLDAAGLRH VVLVSAWAAD RCTAGPLASA KDVAGQVFVT GGETPADMEK AVQLVKETLE GMKGGTSKLG FRGKGSLKEW DTLVARLQE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag ctgtattcat gcgcgttggt gaactagcgt gtcagaagga ttcgttcctt cgagagttga ccgccactgt tgtctcgtgt gagccggcca agacggcagt ggcaacggcg aagaaggcga agggcgccag caacgacact gtggcgtacg acgttgttct gagcgactcg gtcctttttc cagagggcgg tggtcagccc tgcgactatg gcacattacg agtggtgtcc agcgataagg acaagtcgga tccctcgagt gaagccacca ccgtgcccct gatccccatt acgagcgtgc gccgggtcgg ggacacatgc gtgctaacca gtcccaccgc cctccacgtg ggggctacgg tgcaccaaat ggtagactgg ccccgtcggc tcgaccatat gcagcaccac accggccagc acctgctcac ggccattgta gagcgcaagg acacgctaca cttgcctacc gtcagctggt ccctgacgca cccccactgc ttcatccagg tggacgttgg cgcctacgca agcgacgccg cctcgccata ccacacgtac atcacaaagg agaaaaaaat ttcagacgag atggtggcaa aggtggaggc actctgcaac gacgccatca cacgcagcac cgccgtgcac tgcgacgtgt tcgaaaatcg ccaggtgtat gtgcaagagc aggatcgccg gtgtcagagc actgcagcct cggcagacgg cggcgaggaa gcctttcgca gccgtggcat cccagaggac gtgaccggac caattcgcat catctcacta gacgcgatcg acagctgcac ctgctgcggc acccaccttc acacactggc agagctgaag gccatgtacc tcctccacca agaggtgaaa gggaccctag tgaaactgta cttcatcact ggcgaccggg ccgcacagga attccacgcc atgtatcagc gagagcggca gttgatgctg gagttgggtg gcagcagacc caacgacttt gtctccgtgg tgcagcgccg ttccaaagat gcgattaacc ttgagaagct gctgaagagg tggacggtgg agcttgcgag tgtagaagcg cagcgcattg ccgccactgt gtcatcgtca aactccacta ccgtcatggt accccttcgc cgcgacgacg tagagctcga tttcttcaac gaggtccgcg cacggctcga cgcggcaggg cttcgccacg tcgttctcgt ttccgcgtgg gcagcggacc ggtgtacagc aggcccgctc gccagcgcca aggatgtggc gggtcaggtc ttcgtcaccg gcggggagac acctgcggac atggagaagg ctgtccagct ggtgaaggaa acgctagagg ggatgaaggg aggcacctcc aagcttggtt tccgtgggaa gggcagtctg aaggagtggg acaccctcgt ggcacgtctc caggagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t