LetaA.19530.a

J-binding protein 1 (JBP1)

CENTER ID: LetaA.19530.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.19530.a.B3.GE41654 Tet-JBP oxygenase 61 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79401
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP09.1510
UniProt: Q9U6M1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPDSKKVKL DIFNFPTTRE TRTPEEVAES YAEAVKSHPF YDNVHSVVDF YDSGTIKDGR GQIIGVVLRE ALPKYAASMA SELLTSAAVR TSLRSMMFGG EPPLSGIAGY FDYRGSPVEL KSRKTSFTYE HEAAWPAVFP VVDYVSEIYR HVAPERWKAQ NDAIPDLVRI HGTPFSTLTI NSRFRTASHT DVGDFDAGYS CIACLDGQFK GLALSFDDFG INVLLQPRDV MIFDSHHFHS NTEVELSFSG EDWKRLTCVF YYRAALGEPA SYAEYQRRLE KSKTDTRFTP VVHHVRVKEN GTSVNRPSPV YPISQSPFWV PMVAHCLQHC ASAAQCVHEA MTADGSRLAE MMFGESLSTS DGIPLRGEDE KVKANGDSTP RPLSRLGGFS ETNLMVSTAV EKKKYLDSEF LLHCISAQLL DMWKQARARW LELVGKEWAH MLALNPERKD FLWKNQSEMN SAFFDLCEVG KQVMLGLLGK EVALPKEEQA FWIMYAVHLS AACAEELHMP EVAMSLRKLN VKLKDFNFGG TRYFKDMPPE EKKRRMERKQ RIEEARRHGM PSGSHEKRAN WLTNDSFDYQ TEDCVIDYAQ HKWVLPALHA KEVTKTVRTG ELPTTERVVR VLVVIPDPQS KLENVDCKLE VPDMVGSSSE WERLMSSPAV HRVLSAAQRN LQLPDSVTHG NVQTHFAFHS TLPTDIYDFV VLQHVLSRIP DDAQASAYIR RAAALCSGCL FVVETDVQCR QYYTLKYSIR CSYDTVAPLF FQQLHRVCYG TKTARVRTKG ELESLIPTVC CARYKLQGSP LNTTVHVVSP FPSCEVQNLS SALCDRA
NT Sequence
atggagcctg actccaagaa ggttaaactg gatatcttca actttccgac caccagggag acacgtacgc cggaggaagt cgcagagagc tacgcggagg ctgtgaagtc gcaccccttt tatgataacg tgcactccgt tgtcgacttt tatgatagcg gtaccatcaa agatggtcgg gggcaaatca ttggtgttgt gctgcgggaa gctctcccaa agtacgcggc atccatggcg tcggagctgc tcacatccgc tgctgtgcgc acttcgcttc gaagcatgat gttcggtgga gagccgccgt tgagtggtat tgctgggtac ttcgactacc ggggatcacc ggtggaactg aagtcccgca aaacgtcgtt cacgtatgag catgaggcgg catggccggc ggtgttccct gtagtcgact acgtcagcga gatctacagg catgtagcac cggaacggtg gaaagcacaa aacgatgcca ttccagatct tgtgcgcatt cacggtaccc cattctccac gctcaccatc aactcccgct tccgcacagc ctctcacacc gacgtcggcg atttcgatgc cggctacagt tgcattgcct gccttgacgg gcagttcaaa gggctggccc tgtcctttga cgactttggc atcaatgtgc tcttgcaacc gcgtgatgtg atgatatttg actcgcacca ctttcactca aacacggagg tggagttgtc gttttcaggg gaggactgga agcggcttac ctgcgtattc tactaccgcg ccgctctcgg cgagccggca agctacgcgg aataccagcg tcggctggag aagtcaaaga cggatacccg cttcactcct gtggtgcatc acgtgagggt gaaggagaac ggaacaagcg tgaaccgccc atcccctgtc tatcccatct ctcaatcgcc tttttgggtg cctatggtgg cgcattgcct acagcactgt gcatcggcgg cacaatgcgt tcacgaagcc atgacagcgg atgggagccg actagcggag atgatgtttg gagaatcgct gtcgacctca gatggaattc cactgcgtgg agaggatgag aaggtaaaag cgaacggcga cagcacgccg aggccgctct cacgtctggg tgggttcagc gagacgaacc tgatggtgtc cacggcagtg gaaaagaaga agtacctcga cagtgagttc cttcttcact gcatcagtgc acaactgctg gatatgtgga agcaggcacg tgcaaggtgg ctggagctgg tgggcaagga atgggcgcat atgctcgccc tcaatcccga gcgcaaagac tttttgtgga agaaccaatc cgagatgaat tcagcttttt tcgatctctg tgaagtggga aagcaggtta tgctgggtct gctgggcaag gaggtggctc taccaaagga agagcaggcg ttctggatca tgtacgctgt gcacctgagt gcggcgtgcg cggaagagct gcacatgccg gaggtcgcca tgtcgcttcg caagctcaac gtgaagctga aggacttcaa ctttggcggt acgcgatact tcaaagacat gccccccgag gaaaagaagc gcagaatgga gcgcaaacag cgtatcgagg aggcacgccg ccacggcatg ccgtctggct cacacgagaa gcgtgcgaac tggctgacca acgattcatt cgactaccaa acggaggact gtgtgattga ttacgcccag cacaagtggg tgctgcctgc gctgcacgct aaagaggtca cgaagaccgt gcgcactggc gagctgccaa ctacggaaag agtggtgcgg gttctcgttg tgatccctga cccgcaaagc aagttggaga atgtcgactg caagctggag gtgccagaca tggtgggatc ttcatcggag tgggagcgtt tgatgagcag cccagcggtg catcgcgttc tatccgctgc gcagcgcaac ctgcagctgc ctgacagcgt cacgcatggt aacgttcaga ctcactttgc ttttcacagc acgctgccca cggacattta cgactttgtg gtgctgcaac atgtcttgag ccgcattcca gacgacgcgc aggcgtcggc gtatatcagg cgcgccgcag cgctgtgctc tgggtgtctg tttgtggtgg agacggacgt gcaatgtcga caatattaca cgctcaagta ctctattcga tgcagctacg acacggtcgc cccattgttc ttccagcaac tgcaccgggt gtgttacggg acgaaaacgg cacgcgtacg cacaaaggga gaattggagt ctctgatccc tacggtatgc tgtgctcgtt acaagctgca gggctcgccg ctgaacacga ccgttcatgt tgtgtctccc tttccgtctt gcgaggtgca gaacctctcc tctgccctat gtgacagggc ctag
Details for LetaA.19530.a.B3.GE41654
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMG QIIGVVLREA LPKYAASMAS ELLTSAAVRT SLRSMMFGGE PPLSGIAGYF DYRGSPVELK SRKTSFTYEH EAAWPAVFPV VDYVSEIYRH VAPERWKAQN DAIPDLVRIH GTPFSTLTIN SRFRTASHTD VGDFDAGYSC IACLDGQFKG LALSFDDFGI NVLLQPRDVM IFDSHHFHSN TEVELSFSGE DWKRLTCVFY YRAALGEPAS XXXXPASAGV SE
Validated NT Sequence
cttactcact tactccagcc gacgctggta nnnnncnnna gcttgccggc tcgccgagag cggcgcggta gtagaatacg caggtaagcc gcttccagtc ctcccctgaa aacgacaact ccacctccgt gtttgagtga aagtggtgcg agtcaaatat catcacatca cgcggttgca agagcacatt gatgccaaag tcgtcaaagg acagggccag ccctttgaac tgcccgtcaa ggcaggcaat gcaactgtag ccggcatcga aatcgccgac gtcggtgtga gaggctgtgc ggaagcggga gttgatggtg agcgtggaga atggggtacc gtgaatgcgc acaagatctg gaatggcatc gttttgtgct ttccaccgtt ccggtgctac atgcctgtag atctcgctga cgtagtcgac tacagggaac accgccggcc atgccgcctc atgctcatac gtgaacgacg ttttgcggga cttcagttcc accggtgatc cccggtagtc gaagtaccca gcaataccac tcaacggcgg ctctccaccg aacatcatgc ttcgaagcga agtgcgcaca gcagcggatg tgagcagctc cgacgccatg gatgccgcgt actttgggag agcttcccgc agcacaacac caatgatttg ccccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHGQ IIGVVLREAL PKYAASMASE LLTSAAVRTS LRSMMFGGEP PLSGIAGYFD YRGSPVELKS RKTSFTYEHE AAWPAVFPVV DYVSEIYRHV APERWKAQND AIPDLVRIHG TPFSTLTINS RFRTASHTDV GDFDAGYSCI ACLDGQFKGL ALSFDDFGIN VLLQPRDVMI FDSHHFHSNT EVELSFSGED WKRLTCVFYY RAALGEPASY AEYQRRLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggcaaa tcattggtgt tgtgctgcgg gaagctctcc caaagtacgc ggcatccatg gcgtcggagc tgctcacatc cgctgctgtg cgcacttcgc ttcgaagcat gatgttcggt ggagagccgc cgttgagtgg tattgctggg tacttcgact accggggatc accggtggaa ctgaagtccc gcaaaacgtc gttcacgtat gagcatgagg cggcatggcc ggcggtgttc cctgtagtcg actacgtcag cgagatctac aggcatgtag caccggaacg gtggaaagca caaaacgatg ccattccaga tcttgtgcgc attcacggta ccccattctc cacgctcacc atcaactccc gcttccgcac agcctctcac accgacgtcg gcgatttcga tgccggctac agttgcattg cctgccttga cgggcagttc aaagggctgg ccctgtcctt tgacgacttt ggcatcaatg tgctcttgca accgcgtgat gtgatgatat ttgactcgca ccactttcac tcaaacacgg aggtggagtt gtcgttttca ggggaggact ggaagcggct tacctgcgta ttctactacc gcgccgctct cggcgagccg gcaagctacg cggaatacca gcgtcggctg gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat