LetaA.19528.a

J-binding protein 2 (JBP2)

CENTER ID:	LetaA.19528.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19528.a.B2.GE41651	N-term conserved domain		14	166
LetaA.19528.a.B3.GE41652	Tet_JBP oxygenase		245	513

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79435

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP14.0030
UniProt:	B6EU02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLNGLTRVST SSELESILDI VQSSGEIAVV FISPSIGDLE TITSETQRRQ LRIAGIPRGG YTILPAIPLY DDELLQMCER YTAANDYEKA QIRDSLFMRE YPLFAYSVRN HKALFHPADY VSRILQFCSY YVQAPDADVL SLLDRSPFLH ISPIKEICTH IRLIARGTPL APEDSESPAP EQLRFHAESD AEKLAAERAG AMSIATSSGG ASETEQLSLF SGVVPSALFQ KDAVEEVDKD TEETMVDLTG EETVDAVHSF QAEYLTLDGL ELVTKAAIFY DREGEGQRIV AVYIPGGVPE DTCRAAAAVL EPAATKKNLR ALTNGGLPPD TGLVGYYDYL TNPTRHKCRE TEFSRRNWGL LAQSEPLLKH LDKLYSQLAP MHHHLQKVAI PSQYQLCGTV FSTITVNRNF RTAVHTDKGD FRSGLGVLSV INGEFEGCHL AIKKLKKAFQ LKVGDVLLFD TSLEHGNTEV INPEIHWQRT SIVCYLRTGL MSSVCEMERR KHLNRLILQQ LRNTEVLNTT VNINGADSSL PPLFVPTRLA SHLAPVQLAA LGFIVERTEK QSGCVVAMTM GLGKTLVALT LCFSQLHLAP QADILILTPK PIISHWVDEK NKWAMHGLHF PHFVASDGLN SLEFEQQLLE YERQKNNEKP KLGHVFVING EYLAGFLRRF KRFTPLLIIV DEGHRVAAKG NKLTESLDRL RCNLRIVLSG TPLQNDASEL YRLVGWVNKG VGRVLPPKRF QELANDINQF VEGDDGAFYN AVMAQEYIQD WMRGFVFREM ENDLPPLHDY LLICGSSDVQ REYEEKLGLT ETTMTALKAT EHRPHHLSTH PACYLAFISD SYQSMVSGWT VRAQANTSRM RVSQLEEIDT MRLEHYVQMV ENEQLDTFID LSGKMRVLVD IVLRVQARKE KLIIFSLYVG SQDLIHRTLT ALRVCTFTVR GRDSQDRRRR AMQEFSENKD LIVLVLSTKI AAYGLDFTAA NHVVLFDSWW NPQVDAQAIA RAYRRNQRKP VTVYRLISAT ENKFVLSSQT RKIALFKCIL HERTSRQALP DELEDCAANE KDEERRSFWA KLKTTLLAGG TRALLNVYRY QESVRESE

NT Sequence

atgttaaatg ggctgacgcg ggtgagcacc agttctgagc tggagtcgat actggacatc gttcagtcgt ccggcgagat tgcggttgtc ttcatttcgc cgagcattgg ggacttggaa acgattacga gtgaaacaca gcgccgacag ctgcgcattg cgggaatccc tcgcggtgga tacacaattc ttcctgctat tccgttatac gatgacgagc tgcttcagat gtgcgagcgg tacaccgccg cgaacgacta cgagaaggca caaatacgcg acagcttgtt tatgcgagaa tacccactat tcgcctacag cgtgcgcaat cacaaggcat tgtttcaccc tgccgactac gtgagccgga ttctccagtt ttgctcctat tatgtgcaag cgcccgacgc agacgttttg tcgctgctgg acaggagtcc ttttcttcac atctccccga taaaagaaat ttgcacacat atccgcctta ttgcccgcgg caccccactg gccccagaag actccgagtc gccggcccca gagcagctgc gctttcacgc agagtcagac gctgagaagc ttgcagccga gcgagcgggc gctatgtcca tcgcaaccag tagtggtggc gcctcagaga cggagcagct ctctctcttc agcggcgttg tgccgtcggc gctgtttcag aaagacgcag ttgaagaagt tgataaggac accgaagaaa ccatggtgga tctcaccggc gaggagacag tggacgcagt gcacagcttt caggcagagt acttgacact ggatgggctt gagctggtga caaaagcggc catcttctac gaccgcgaag gggaaggcca gcgcatcgtt gctgtgtata ttccaggagg ggtaccagag gacacctgcc gcgcagcagc agcagtcctg gagccagcag cgacaaaaaa aaacttgcgg gcacttacga acggtggact tccaccagac accggactcg taggctacta cgactacctt acgaatccca cccggcacaa gtgtagagag acggaattca gccgtaggaa ctggggcctc ttagcccaga gtgagccact gctgaagcat ctcgataagc tgtacagcca gttggctcca atgcaccatc atctgcagaa ggtggccatc ccgtcacagt accagctgtg cggaacggtg ttcagcacta tcactgtcaa cagaaacttt cgcaccgccg tgcacaccga caagggtgac tttcgaagcg gcttgggggt tctctcagtt atcaacggcg agtttgaggg atgccatctc gccatcaaaa agctcaagaa ggcatttcag ctcaaagtcg gcgacgttct gctttttgac acgtctctgg agcacggcaa cacggaggta ataaacccgg aaattcactg gcagcgaacc agcattgtgt gctacctgcg cactggacta atgtcttctg tgtgcgagat ggagcgacgt aagcacctga atcggctcat tctccagcaa ttgcgcaaca cggaggttct caacacgact gtgaacatca acggagccga cagcagcctg ccgccgctgt ttgtgccgac ccgcctggcg agccatttgg cgccagtgca gcttgctgca ctcggcttca ttgtggagcg gacagagaag cagagtgggt gtgtggtggc tatgacgatg ggattgggca aaacgctcgt ggcactcacg ctgtgcttct cacagttgca tcttgcaccg caggctgaca ttctcattct cacgccaaag ccgatcatca gtcactgggt ggacgaaaag aacaagtggg ctatgcacgg gctccacttt ccacacttcg tcgcctctga cggactcaac tcgttggagt tcgagcagca gcttcttgag tacgagcgtc agaaaaacaa cgagaaaccc aagttgggtc acgtcttcgt catcaatggc gagtatctgg cagggtttct gcggcgcttc aaacgattca caccgttgct gattattgtg gacgaaggcc accgagtggc tgcaaagggg aataaattaa cggagtcact cgatcgccta cggtgcaacc tccgtatcgt gctatccggc acgccgttgc aaaacgacgc cagcgaactt taccggctag tgggatgggt gaacaaaggc gtcggcagag tactgccgcc gaagcgtttc caggagctgg cgaatgacat caaccagttc gtcgagggcg acgatggggc cttctataac gcggtgatgg cgcaggagta catccaggac tggatgcgtg ggtttgtgtt tcgtgaaatg gaaaatgatt tgccaccact gcatgattac ctgttgatct gcggttcctc tgacgtgcag cgtgagtatg aggagaagtt gggcctcaca gaaacaacca tgactgccct gaaagccaca gagcatcgac ctcaccacct ctctacgcac cctgcgtgtt acctggcctt catatccgac agctaccaat caatggtaag cggttggaca gtgcgcgcgc aggcgaatac gtcacggatg cgagtgtctc agctggagga gatcgatacg atgcgattgg agcattacgt gcagatggtt gagaacgagc aacttgacac cttcatcgac ttgagcggca agatgcgtgt gctcgtcgat attgtgctgc gcgtgcaagc ccgcaaagag aagctcatca ttttctccct ctacgtgggc tcgcaggacc tgattcatcg cactctgaca gcgctgcggg tctgcacatt tactgttcgc ggccgtgact cacaagaccg ccgccgccgc gccatgcagg agttcagtga gaacaaagac ctcatcgtgt tggtgttatc cacaaagatt gccgcatatg gtcttgactt tacggctgcg aatcacgttg ttttgtttga ttcgtggtgg aacccgcagg tggacgcgca ggctatcgcg cgcgcgtaca ggcgaaatca acggaagccg gtgactgtgt atcgccttat ctcggcaact gaaaacaagt ttgtgctgag ttcacagacc cgaaaaattg ccctgttcaa atgcatcctc cacgagcgca cgagtcggca ggcgttgccg gacgagctcg aggattgcgc agcgaacgag aaggatgaag aaaggcgaag cttctgggcg aagctgaaga caactcttct agctggaggt actcgggcgt tgttgaatgt gtatcgctac caggagagcg ttcgcgaaag cgaatga

Details for LetaA.19528.a.B2.GE41651

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML ESILDIVQSS GEIAVVFISP SIGDLETITS ETQRRQLRIA GIPRGGYTIL PAIPLYDDEL LQMCERYTAA NDYEKAQIRD SLFMREYPLF AYSVRNHKAL FHPADYVSRI LQFCSYYVQA PDADVLSLLD RSPFLHISPI KEICTHIRLI AR

Validated NT Sequence

atcttactca cttagcgggc aataaggcgg atatgtgtgc aaatttcttt tatcggggag atgtgaagaa aaggactcct gtccagcagc gacaaaacgt ctgcgtcggg cgcttgcaca taataggagc aaaactggag aatccggctc acgtagtcgg cagggtgaaa caatgccttg tgattgcgca cgctgtaggc gaatagtggg tattctcgca taaacaagct gtcgcgtatt tgtgccttct cgtagtcgtt cgcggcggtg taccgctcgc acatctgaag cagctcgtca tcgtataacg gaatagcagg aagaattgtg tatccaccgc gagggattcc cgcaatgcgc agctgtcggc gctgtgtttc actcgtaatc gtttccaagt ccccaatgct cggcgaaatg aagacaaccg caatctcgcc ggacgactga acgatgtcca gtatcgactc cagcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHLE SILDIVQSSG EIAVVFISPS IGDLETITSE TQRRQLRIAG IPRGGYTILP AIPLYDDELL QMCERYTAAN DYEKAQIRDS LFMREYPLFA YSVRNHKALF HPADYVSRIL QFCSYYVQAP DADVLSLLDR SPFLHISPIK EICTHIRLIA R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctggagt cgatactgga catcgttcag tcgtccggcg agattgcggt tgtcttcatt tcgccgagca ttggggactt ggaaacgatt acgagtgaaa cacagcgccg acagctgcgc attgcgggaa tccctcgcgg tggatacaca attcttcctg ctattccgtt atacgatgac gagctgcttc agatgtgcga gcggtacacc gccgcgaacg actacgagaa ggcacaaata cgcgacagct tgtttatgcg agaataccca ctattcgcct acagcgtgcg caatcacaag gcattgtttc accctgccga ctacgtgagc cggattctcc agttttgctc ctattatgtg caagcgcccg acgcagacgt tttgtcgctg ctggacagga gtccttttct tcacatctcc ccgataaaag aaatttgcac acatatccgc cttattgccc gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for LetaA.19528.a.B3.GE41652

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Validated AA Sequence

MAHHHHHHMX XLTGEETVDA VHSFQAEYLT LDGLELVTKA AIFYDREGEG QRIVAVYIPG GVPEDTCRAA AAVLEPAATK KNLRALTNGG LPPDTGLVGY YDYLTNPTRH KCRETEFSRR NWGLLAQSEP LLKHLDKLYS QLAPMHHHLQ KVAIPSQYQL CGTVFSTITV NRNFRTAVHT DKGDFRSGLG VLSVINGEFE GCHLAIKKLK KAFQLKVGDV LLFDTSLEHG NTEVINPEIH WQRTSIVCYL RTGLXXXXCE M

Validated NT Sequence

tccatctcgc acannnnnnn nattagccca gtgcgcaggt agcacacaat gctggttcgc tgccagtgaa tttccgggtt tattacctcc gtgttgccgt gctccagaga cgtgtcaaaa agcagaacgt cgccgacttt gagctgaaat gccttcttga gctttttgat ggcgagatgg catccctcaa actcgccgtt gataactgag agaaccccca agccgcttcg aaagtcacct ttgtcggtgt gcacggcggt gcgaaagttt ctgttgacag tgatagtgct gaacaccgtt ccgcacagct ggtactgtga cgggatggcc accttttgca gatgatggtg cattggagcc aactggctgt acagcttatc gagatgcttc agcagtggct cactctgggc taagaggccc cagttcctac ggctgaattc cgtctctcta cacttgtgcc gggtgggatt cgtaaggtag tcgtagtagc ctacgagtcc ggtgtctggt ggaagtccac cgttcgtaag tgcccgcaag tttttttttg tcgctgctgg ctccaggact gctgctgctg cgcggcaggt gtcctctggt acccctcctg gaatatacac agcaacgatg cgctggcctt ccccttcgcg gtcgtagaag atggccgctt ttgtcaccag ctcaagccca tccagtgtca agtactctgc ctgaaagctg tgcactgcgt ccactgtctc ctcgccggtg agatnnnnca tatggtggtg gtggtggtga gccatggtat at

Expected Protein Sequence

MAHHHHHHMV DLTGEETVDA VHSFQAEYLT LDGLELVTKA AIFYDREGEG QRIVAVYIPG GVPEDTCRAA AAVLEPAATK KNLRALTNGG LPPDTGLVGY YDYLTNPTRH KCRETEFSRR NWGLLAQSEP LLKHLDKLYS QLAPMHHHLQ KVAIPSQYQL CGTVFSTITV NRNFRTAVHT DKGDFRSGLG VLSVINGEFE GCHLAIKKLK KAFQLKVGDV LLFDTSLEHG NTEVINPEIH WQRTSIVCYL RTGLMSSVCE MERRKHLNRL ILQQLRN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg atctcaccgg cgaggagaca gtggacgcag tgcacagctt tcaggcagag tacttgacac tggatgggct tgagctggtg acaaaagcgg ccatcttcta cgaccgcgaa ggggaaggcc agcgcatcgt tgctgtgtat attccaggag gggtaccaga ggacacctgc cgcgcagcag cagcagtcct ggagccagca gcgacaaaaa aaaacttgcg ggcacttacg aacggtggac ttccaccaga caccggactc gtaggctact acgactacct tacgaatccc acccggcaca agtgtagaga gacggaattc agccgtagga actggggcct cttagcccag agtgagccac tgctgaagca tctcgataag ctgtacagcc agttggctcc aatgcaccat catctgcaga aggtggccat cccgtcacag taccagctgt gcggaacggt gttcagcact atcactgtca acagaaactt tcgcaccgcc gtgcacaccg acaagggtga ctttcgaagc ggcttggggg ttctctcagt tatcaacggc gagtttgagg gatgccatct cgccatcaaa aagctcaaga aggcatttca gctcaaagtc ggcgacgttc tgctttttga cacgtctctg gagcacggca acacggaggt aataaacccg gaaattcact ggcagcgaac cagcattgtg tgctacctgc gcactggact aatgtcttct gtgtgcgaga tggagcgacg taagcacctg aatcggctca ttctccagca attgcgcaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat