LetaA.19511.a

KREN3

CENTER ID: LetaA.19511.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.19511.a.B1.GE40879 full length 1 614
LetaA.19511.a.B2.GE40880 RNase III-like catalytic-like domain 215 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LtaP36.0940

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLHRTPPWR LFYRTPHALC MHRREVVSVT RSSSSRPTPC EAEKLFSSPM VHAWSVNSVQ ETVISRSAIS RAERIVTEAD NPRSDKRAFY DSEAKLCLVC NARLEGSYST HTHSVDHKPR IALLKRTIGV ILTFLEQSTA SPSSPLLQES DQFLATAGSK RFLQSVTAHR STPTGVGTSC FHASYAESPL FAEDGVPSRA LLYHCPRKLR EFDLVDVILR RWWNTLHNPP PKRGSLSFDR LLSLSSTELK IRRWRVRYLL HFLKSRGVLR SCLTLRSEEA NEAPPPNERI HRSETFERLE MVGDFVFKSL TPDRLHTVFP ADEGGLTTEL QCFERALDSN HGLLAIYDYL GLDDIAGCFL ANNKAKADVV EAVIGELKVL LWSTEVVWGM EYFSVPGERA THVYLRALVA HTIAELGHIV LMWQLESTLR NCRDFILKHT VEEYMREKGN EHRAKGDSQG CDVPAYLPKY APLPPLLAWD RRRPGVSLRR GQVHFSRNQP RDSIPPSPQD PFRRLAPTAG FIPEAILKHY SVGQKTWSLS ACTTALHALC GSLTPSAAPL NSGETADALS PNSKSISLPL STKPQPPSTV PTSRGVPQNV LRALVTANCT VIRN
NT Sequence
atgcgtcttc atcgcactcc cccgtggcga cttttttacc gcacccctca tgccttgtgc atgcaccgtc gcgaggtcgt gtcagtgaca cgctcttctt cctcgcgccc aacgccatgt gaggctgaga agcttttttc cagccccatg gtgcacgcgt ggtcggtgaa ttctgttcaa gagacggtga tctcccggag cgccatcagt cgagcagagc gcattgtaac agaggccgac aatccacgct ccgataagag ggctttctac gacagtgagg ccaagctgtg tcttgtgtgc aacgcccgtc tggagggaag ctacagcacg cacacccaca gtgtcgatca caagccgcgc attgcacttt tgaagcgcac gattggtgtg attctcacat ttcttgagca gagcaccgca tcaccatcgt cgccactgct gcaggagtcg gaccaatttt tggccacggc tggcagcaaa cgcttcttac agtcagtgac agctcacaga tcaacgccta ccggcgtagg cacctcgtgc ttccacgctt cgtatgccga gtcaccgtta tttgctgaag atggtgtgcc aagtcgcgct ctcctttatc actgcccgcg aaagctgcgc gagtttgacc tggtagacgt gatcctgcga cgctggtgga acaccttgca taatccacca cctaagcgtg gtagtctttc gtttgaccgt ctcctctcac tttcctcgac tgagctgaag atacgacgat ggcgtgtgcg gtacctcctc cactttctca agtcacgtgg ggttctccgc tcttgtttga cattgaggtc cgaggaagcg aatgaggcgc cgccccccaa cgagcgtatt caccgtagtg aaacttttga gcgactcgag atggttggcg acttcgtctt taagagcctc actcccgatc gattgcacac cgtcttccca gctgatgaag gcggacttac gaccgaactc cagtgctttg agcgggcgtt ggattcgaat catggtctgc tggcgatcta cgactacctc ggcttggatg acatagcggg ctgctttctg gccaacaaca aggcgaaggc cgacgttgtg gaggccgtta ttggggagct gaaggttctg ttgtggtcga ccgaggtggt gtggggtatg gagtattttt ccgtacctgg ggaacgcgct acgcatgttt atctgcgcgc gctggtggcg cacaccatcg ctgagctggg gcatattgtg ctgatgtggc aactggagtc gacattacgt aactgtcgag acttcattct caaacataca gtggaggaat acatgaggga gaaagggaac gagcaccgtg caaagggcga cagtcaaggg tgcgatgtcc ctgcgtacct acccaaatac gcaccgctgc caccgttgct cgcgtgggat cggcgccgcc cgggcgtgtc gctgcggagg ggtcaggtgc acttctcaag gaaccagcct cgagacagca taccgccttc tcctcaagac ccgtttcgca gacttgctcc gactgcgggc ttcattccag aggctattct gaagcactac tctgttggcc aaaaaacgtg gagtttaagt gcctgtacaa ctgctcttca cgccctctgc ggatctctca caccatcggc ggcgccgctt aacagtggtg aaaccgctga cgctttatca ccgaactcga agtcgatatc gttacccctc tctacaaaac ctcagccgcc ctcaaccgtg ccgacgtccc ggggcgtccc tcagaatgtg ctacgagctc tggtgactgc gaattgcacg gtgattcgta actag
Details for LetaA.19511.a.B1.GE40879
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR LHRTPPWRLF YRTPHALCMH RREVVSVTRS SSSRPTPCEA EKLFSSPMVH AWSVNSVQET VISRSAISRA ERIVTEADNP RSDKRAFYDS EAKLCLVCNA RLEGSYSTHT HSVDHKPRIA LLKRTIGVIL TFLEQSTASP SSPLLQESDQ FLATAGSKRF LQSVTAHRST PTGVGTSCFH ASYAESPLFA EDGVPSRALL YHCPRKLREF DLVDVILRRW WNTLHNPPPK RGSLSFDRLL SLSSTELKIR RWRVRYLLHF LKSRGVLRSC LTLRSEEANE APPPNERIHR SETFERLEMV GDFVFKSLTP DRLHTVFPAD EGGLTTELQC FERALDSNHG LLAIYDYLGL DDIAGCFLAN NKAKADVVEA VIGELKVLLW STEVVWGMEY FSVPGERATH VYLRALVAHT IAELGHIVLM WQLESTLRNC RDFILKHTVE EYMREKGNEH RAKGDSQGCD VPAYLPKYAP LPPLLAWDRR RPGVSLRRGQ VHFSRNQPRD SIPPSPQDPF RRLAPTAGFI PEAILKHYSV GQKTWSLSAC TTALHALCGS LTPSAAPLNS GETADALSPN SKSISLPLST KPQPPSTVPT SRGVPQNVLR ALVTANCTVI RN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtc ttcatcgcac tcccccgtgg cgactttttt accgcacccc tcatgccttg tgcatgcacc gtcgcgaggt cgtgtcagtg acacgctctt cttcctcgcg cccaacgcca tgtgaggctg agaagctttt ttccagcccc atggtgcacg cgtggtcggt gaattctgtt caagagacgg tgatctcccg gagcgccatc agtcgagcag agcgcattgt aacagaggcc gacaatccac gctccgataa gagggctttc tacgacagtg aggccaagct gtgtcttgtg tgcaacgccc gtctggaggg aagctacagc acgcacaccc acagtgtcga tcacaagccg cgcattgcac ttttgaagcg cacgattggt gtgattctca catttcttga gcagagcacc gcatcaccat cgtcgccact gctgcaggag tcggaccaat ttttggccac ggctggcagc aaacgcttct tacagtcagt gacagctcac agatcaacgc ctaccggcgt aggcacctcg tgcttccacg cttcgtatgc cgagtcaccg ttatttgctg aagatggtgt gccaagtcgc gctctccttt atcactgccc gcgaaagctg cgcgagtttg acctggtaga cgtgatcctg cgacgctggt ggaacacctt gcataatcca ccacctaagc gtggtagtct ttcgtttgac cgtctcctct cactttcctc gactgagctg aagatacgac gatggcgtgt gcggtacctc ctccactttc tcaagtcacg tggggttctc cgctcttgtt tgacattgag gtccgaggaa gcgaatgagg cgccgccccc caacgagcgt attcaccgta gtgaaacttt tgagcgactc gagatggttg gcgacttcgt ctttaagagc ctcactcccg atcgattgca caccgtcttc ccagctgatg aaggcggact tacgaccgaa ctccagtgct ttgagcgggc gttggattcg aatcatggtc tgctggcgat ctacgactac ctcggcttgg atgacatagc gggctgcttt ctggccaaca acaaggcgaa ggccgacgtt gtggaggccg ttattgggga gctgaaggtt ctgttgtggt cgaccgaggt ggtgtggggt atggagtatt tttccgtacc tggggaacgc gctacgcatg tttatctgcg cgcgctggtg gcgcacacca tcgctgagct ggggcatatt gtgctgatgt ggcaactgga gtcgacatta cgtaactgtc gagacttcat tctcaaacat acagtggagg aatacatgag ggagaaaggg aacgagcacc gtgcaaaggg cgacagtcaa gggtgcgatg tccctgcgta cctacccaaa tacgcaccgc tgccaccgtt gctcgcgtgg gatcggcgcc gcccgggcgt gtcgctgcgg aggggtcagg tgcacttctc aaggaaccag cctcgagaca gcataccgcc ttctcctcaa gacccgtttc gcagacttgc tccgactgcg ggcttcattc cagaggctat tctgaagcac tactctgttg gccaaaaaac gtggagttta agtgcctgta caactgctct tcacgccctc tgcggatctc tcacaccatc ggcggcgccg cttaacagtg gtgaaaccgc tgacgcttta tcaccgaact cgaagtcgat atcgttaccc ctctctacaa aacctcagcc gccctcaacc gtgccgacgt cccggggcgt ccctcagaat gtgctacgag ctctggtgac tgcgaattgc acggtgattc gtaactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LetaA.19511.a.B2.GE40880
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV DVILRRWWNT LHNPPPKRGS LSFDRLLSLS STELKIRRWR VRYLLHFLKS RGVLRSCLTL RSEEANEAPP PNERIHRSET FERLEMVGDF VFKSLTPDRL HTVFPADEGG LTTELQCFER ALDSNHGLLA IYDYLGLDDI AGCFLANNKA KADVVEAVIG ELKVLLWSTE VVWGMEYFSV PGERATHVYL RALVAHTIAE LGHIVLMWQL ESTLRNCRDF ILKHTVEEYM REKGNEHRAK GDSQGCDVPA XLPKYA
Validated NT Sequence
atcttactca cttatgcgta tttgggtagg tnngcaggga catcgcaccc ttgactgtcg ccctttgcac ggtgctcgtt ccctttctcc ctcatgtatt cctccactgt atgtttgaga atgaagtctc gacagttgcg taatgtcgac tccagttgcc acatcagcac aatatgcccc agctcagcga tggtgtgcgc caccagcgcg cgcagataaa catgcgtagc gcgttcccca ggtacggaaa aatactccat accccacacc acctcggtcg accacaacag aaccttcagc tccccaataa cggcctccac aacgtcggcc ttcgccttgt tgttggccag aaagcagccc gctatgtcat ccaagccgag gtagtcgtag atcgccagca gaccatgatt cgaatccaac gcccgctcaa agcactggag ttcggtcgta agtccgcctt catcagctgg gaagacggtg tgcaatcgat cgggagtgag gctcttaaag acgaagtcgc caaccatctc gagtcgctca aaagtttcac tacggtgaat acgctcgttg gggggcggcg cctcattcgc ttcctcggac ctcaatgtca aacaagagcg gagaacccca cgtgacttga gaaagtggag gaggtaccgc acacgccatc gtcgtatctt cagctcagtc gaggaaagtg agaggagacg gtcaaacgaa agactaccac gcttaggtgg tggattatgc aaggtgttcc accagcgtcg caggatcacg tctaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHVD VILRRWWNTL HNPPPKRGSL SFDRLLSLSS TELKIRRWRV RYLLHFLKSR GVLRSCLTLR SEEANEAPPP NERIHRSETF ERLEMVGDFV FKSLTPDRLH TVFPADEGGL TTELQCFERA LDSNHGLLAI YDYLGLDDIA GCFLANNKAK ADVVEAVIGE LKVLLWSTEV VWGMEYFSVP GERATHVYLR ALVAHTIAEL GHIVLMWQLE STLRNCRDFI LKHTVEEYMR EKGNEHRAKG DSQGCDVPAY LPKYA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtagacg tgatcctgcg acgctggtgg aacaccttgc ataatccacc acctaagcgt ggtagtcttt cgtttgaccg tctcctctca ctttcctcga ctgagctgaa gatacgacga tggcgtgtgc ggtacctcct ccactttctc aagtcacgtg gggttctccg ctcttgtttg acattgaggt ccgaggaagc gaatgaggcg ccgcccccca acgagcgtat tcaccgtagt gaaacttttg agcgactcga gatggttggc gacttcgtct ttaagagcct cactcccgat cgattgcaca ccgtcttccc agctgatgaa ggcggactta cgaccgaact ccagtgcttt gagcgggcgt tggattcgaa tcatggtctg ctggcgatct acgactacct cggcttggat gacatagcgg gctgctttct ggccaacaac aaggcgaagg ccgacgttgt ggaggccgtt attggggagc tgaaggttct gttgtggtcg accgaggtgg tgtggggtat ggagtatttt tccgtacctg gggaacgcgc tacgcatgtt tatctgcgcg cgctggtggc gcacaccatc gctgagctgg ggcatattgt gctgatgtgg caactggagt cgacattacg taactgtcga gacttcattc tcaaacatac agtggaggaa tacatgaggg agaaagggaa cgagcaccgt gcaaagggcg acagtcaagg gtgcgatgtc cctgcgtacc tacccaaata cgcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat