SSGCID: Target Page

LetaA.19509.a

KREPA2

CENTER ID:	LetaA.19509.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19509.a.B1.GE40710	full length		1	630

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LtaP36.7190

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPMNRRIGVF AATAAACRCA LRRLRHVSPS SMQALGAVAS AKGTSHAALW GPVRNQSAIP DRRFHCSVCK KSFRLEMAAK LHLQQVHSGE GTVEAGAGPG QGEEQTLQTP VGVFRNAPPQ APTVVTQIVP HSNERAPRRE KPAPKPLHQA EREVPSLVME KMLSVWDDIG VKRMGNEFVH SSMVMRVFAA RPSDSSEPVY EVMNPEGESP FEGGSSTADT ASPITRKNEN AKFYSIAVGD AFAMASDEAL GPLRPARCPN PFLRKLAKDT AKISPRVESR EQQTAPVTPF GQLPMFGQAA SPATVPAHSA SEEPSTTTNS VVSSPFAAGS NNSPFAGTMS FPLSGQGLFP FDSLQGPPEP AAAPPGIAEG SGASSEAPAS PFASASGALP PSPFAAAPSP FTESSLATPS YAVGDAATMA RSTEASDGGF FPSFSTSSAT APAEAVTTPT HVCIVCEKNF LTYDGLRMHS KAKHGKELPK ELPKGRGSEK REVPDLPAFI PSPVDLTMTS PFGLSAQRSA WTEVELKPYA QSVSNITVVG RVLDTEFSSD GSLLFSLFVP NSDENDSEVI PVRCTSDALR TLGRKLGYGD LVFACGSLRI LPFTDNKTQK KYSSAVVHVA LPTGMISKLN

NT Sequence

ATGCCCATGA ATAGGCGAAT CGGAGTCTTT GCTGCTACGG CTGCTGCGTG CCGCTGCGCG CTGCGCAGAC TGCGCCACGT ATCGCCTAGT TCTATGCAAG CGTTGGGCGC TGTCGCCTCC GCGAAAGGCA CCTCACATGC CGCTCTATGG GGGCCTGTAC GGAATCAAAG TGCGATCCCG GACCGCCGTT TCCACTGCAG CGTTTGCAAG AAGTCGTTTC GCTTGGAGAT GGCGGCAAAG CTGCACCTTC AGCAGGTGCA CAGCGGTGAG GGCACAGTGG AGGCAGGTGC GGGGCCCGGA CAGGGGGAGG AGCAGACGCT GCAGACACCG GTGGGGGTGT TTCGGAATGC ACCTCCGCAG GCACCGACCG TCGTGACGCA GATCGTTCCA CATTCTAACG AGCGCGCTCC GCGTCGTGAG AAGCCTGCGC CAAAGCCACT TCACCAAGCC GAACGCGAGG TGCCAAGTCT TGTGATGGAA AAGATGCTCT CTGTCTGGGA TGACATAGGT GTGAAGCGGA TGGGGAATGA GTTTGTTCAC AGTAGTATGG TGATGCGAGT GTTTGCTGCT CGTCCGTCCG ATAGCTCCGA GCCGGTGTAT GAAGTTATGA ATCCAGAGGG TGAGAGCCCG TTCGAAGGTG GCTCGTCTAC CGCGGATACG GCTAGTCCTA TTACGAGGAA GAACGAAAAC GCCAAATTTT ACAGCATCGC TGTCGGTGAC GCGTTTGCGA TGGCGTCTGA CGAGGCTTTG GGCCCATTGC GCCCCGCTCG GTGCCCGAAC CCATTTCTCC GAAAACTAGC AAAGGATACG GCAAAAATTT CCCCGAGGGT GGAATCGAGA GAACAGCAAA CGGCACCGGT AACGCCTTTT GGACAGCTCC CGATGTTTGG GCAAGCTGCT TCACCTGCTA CGGTCCCAGC TCACAGCGCC TCCGAGGAAC CATCGACCAC GACGAATAGT GTGGTTTCTT CTCCCTTCGC CGCCGGCTCC AATAATTCAC CTTTTGCGGG GACCATGAGC TTTCCATTGT CTGGGCAAGG GCTCTTTCCG TTTGACTCGT TGCAGGGTCC ACCAGAGCCG GCCGCTGCTC CACCCGGGAT CGCCGAGGGA TCGGGGGCTT CCTCTGAGGC ACCGGCTTCC CCGTTTGCTT CTGCTTCTGG TGCTCTTCCT CCATCCCCGT TTGCCGCAGC TCCAAGCCCT TTTACCGAAT CTTCTTTGGC AACCCCCTCG TATGCCGTGG GGGACGCAGC CACGATGGCA AGAAGCACCG AGGCTTCTGA CGGCGGCTTC TTCCCCTCCT TCTCCACGTC TTCTGCGACC GCGCCGGCTG AGGCGGTGAC TACCCCTACG CATGTATGCA TCGTTTGCGA GAAGAACTTT TTGACGTATG ATGGACTTCG AATGCACAGT AAGGCCAAAC ATGGAAAGGA GCTGCCAAAG GAGCTGCCAA AGGGGCGGGG AAGTGAGAAA CGTGAGGTTC CGGACTTACC CGCCTTCATT CCTAGTCCGG TGGATCTCAC GATGACATCA CCCTTTGGTT TGTCGGCGCA GCGATCGGCG TGGACGGAGG TGGAGCTAAA ACCGTACGCG CAGTCCGTGA GTAACATTAC GGTGGTGGGG CGGGTGCTGG ATACCGAATT TTCGAGCGAT GGATCGTTGC TCTTTTCTCT TTTTGTCCCC AATTCGGACG AAAATGATTC TGAGGTGATT CCCGTTCGCT GCACTTCAGA CGCATTGAGA ACGCTTGGTA GAAAGCTCGG GTATGGCGAT CTTGTGTTCG CATGTGGCTC GCTGCGCATT TTACCGTTCA CGGATAATAA AACACAGAAG AAATACTCCT CGGCAGTCGT GCACGTCGCT CTCCCAACTG GAATGATTTC GAAACTGAAC TGA

Details for LetaA.19509.a.B1.GE40710

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	68kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP MNRRIGVFAA TAAACRCALR RLRHVSPSSM QALGAVASAK GTSHAALWGP VRNQSAIPDR RFHCSVCKKS FRLEMAAKLH LQQVHSGEGT VEAGAGPGQG EEQTLQTPVG VFRNAPPQAP TVVTQIVPHS NERAPRREKP APKPLHQAER EVPSLVMEKM LSVWDDIGVK RMGNEFVHSS MVMRVFAARP SDSSEPVYEV MNPEGESPFE GGSSTADTAS PITRKNENAK FYSIAVGDAF AMASDEALGP LRPARCPNPF LRKLAKDTAK ISPRVESREQ QTAPVTPFGQ LPMFGQAASP ATVPAHSASE EPSTTTNSVV SSPFAAGSNN SPFAGTMSFP LSGQGLFPFD SLQGPPEPAA APPGIAEGSG ASSEAPASPF ASASGALPPS PFAAAPSPFT ESSLATPSYA VGDAATMARS TEASDGGFFP SFSTSSATAP AEAVTTPTHV CIVCEKNFLT YDGLRMHSKA KHGKELPKEL PKGRGSEKRE VPDLPAFIPS PVDLTMTSPF GLSAQRSAWT EVELKPYAQS VSNITVVGRV LDTEFSSDGS LLFSLFVPNS DENDSEVIPV RCTSDALRTL GRKLGYGDLV FACGSLRILP FTDNKTQKKY SSAVVHVALP TGMISKLN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca tgaataggcg aatcggagtc tttgctgcta cggctgctgc gtgccgctgc gcgctgcgca gactgcgcca cgtatcgcct agttctatgc aagcgttggg cgctgtcgcc tccgcgaaag gcacctcaca tgccgctcta tgggggcctg tacggaatca aagtgcgatc ccggaccgcc gtttccactg cagcgtttgc aagaagtcgt ttcgcttgga gatggcggca aagctgcacc ttcagcaggt gcacagcggt gagggcacag tggaggcagg tgcggggccc ggacaggggg aggagcagac gctgcagaca ccggtggggg tgtttcggaa tgcacctccg caggcaccga ccgtcgtgac gcagatcgtt ccacattcta acgagcgcgc tccgcgtcgt gagaagcctg cgccaaagcc acttcaccaa gccgaacgcg aggtgccaag tcttgtgatg gaaaagatgc tctctgtctg ggatgacata ggtgtgaagc ggatggggaa tgagtttgtt cacagtagta tggtgatgcg agtgtttgct gctcgtccgt ccgatagctc cgagccggtg tatgaagtta tgaatccaga gggtgagagc ccgttcgaag gtggctcgtc taccgcggat acggctagtc ctattacgag gaagaacgaa aacgccaaat tttacagcat cgctgtcggt gacgcgtttg cgatggcgtc tgacgaggct ttgggcccat tgcgccccgc tcggtgcccg aacccatttc tccgaaaact agcaaaggat acggcaaaaa tttccccgag ggtggaatcg agagaacagc aaacggcacc ggtaacgcct tttggacagc tcccgatgtt tgggcaagct gcttcacctg ctacggtccc agctcacagc gcctccgagg aaccatcgac cacgacgaat agtgtggttt cttctccctt cgccgccggc tccaataatt caccttttgc ggggaccatg agctttccat tgtctgggca agggctcttt ccgtttgact cgttgcaggg tccaccagag ccggccgctg ctccacccgg gatcgccgag ggatcggggg cttcctctga ggcaccggct tccccgtttg cttctgcttc tggtgctctt cctccatccc cgtttgccgc agctccaagc ccttttaccg aatcttcttt ggcaaccccc tcgtatgccg tgggggacgc agccacgatg gcaagaagca ccgaggcttc tgacggcggc ttcttcccct ccttctccac gtcttctgcg accgcgccgg ctgaggcggt gactacccct acgcatgtat gcatcgtttg cgagaagaac tttttgacgt atgatggact tcgaatgcac agtaaggcca aacatggaaa ggagctgcca aaggagctgc caaaggggcg gggaagtgag aaacgtgagg ttccggactt acccgccttc attcctagtc cggtggatct cacgatgaca tcaccctttg gtttgtcggc gcagcgatcg gcgtggacgg aggtggagct aaaaccgtac gcgcagtccg tgagtaacat tacggtggtg gggcgggtgc tggataccga attttcgagc gatggatcgt tgctcttttc tctttttgtc cccaattcgg acgaaaatga ttctgaggtg attcccgttc gctgcacttc agacgcattg agaacgcttg gtagaaagct cgggtatggc gatcttgtgt tcgcatgtgg ctcgctgcgc attttaccgt tcacggataa taaaacacag aagaaatact cctcggcagt cgtgcacgtc gctctcccaa ctggaatgat ttcgaaactg aactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat