LetaA.19505.a

KREX1

CENTER ID: LetaA.19505.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.19505.a.B3.GE40877 endo-exonuclease-phosphatase 683 996
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.19505.a.B3.PS38256 endo-exonuclease-phosphatase 683 996

External Resources

RESOURCE REFERENCE ID
EuPathDB: LtaP26.0800

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGVLARSAC RLTSFQKGTR LADVYQLLIT KKPVEYDYVA IDVNAFVGGA IRITKSLSTE QRRNKEASRH VSNSIMQILR RIVCRHSLLL AFDGPDTFAK AYKLRTTLST RRLDMQTQRL PGGTLMRAVE DRIVKTMPWG RGLIPSEVVV SGVNVEGPVE RKVTAWALDL ACRDGEQHIS KSMCLIGAGE LWMNMLALTP FFQGTSILHG SSDLRHMTLS DSLTWLELGD TLLTGDASVI TAARTDALLL YLLCHGCSTT NLSPLSGSNF VVLMETYHTR RREAAVAADP AAGRPTFQLI REQPNGTLVL DVQALYLLFV AEKDMAATTQ SLAALPVPEK GESPHAAPLT TFSVPVSCSG GSSAEEQMRD SRVSDMLYGS YLSHLLNSHH VFCHGEMLDR FRVPAYMEAE VRMGSIGPSG AAGSRASVIV VGNWIRFLLH CLQRGGRYAF ASDTGVELSD SISKCASHAT RASNVSGASS SNDPAAPGTT AYYPFWSSTQ PLTAAEFTIL STPNSGILDP LLLDYVPGVT PALRSELPRA LTCVQANLPE LRRSLQHFFA YATTDRLHPS LCLAPSYHWI QNEKSKVWSM RYVDLGVVSR QQGVRHARSL LPGMGIEQEA PKEGAVVFDA ELEMWTSQPR RFAALSASEQ MREQQEHTHA DGPSSSCMAA TAVSSADVAE VPVLKVLTWN VMFDRYSNQP TPLGMPGIDW CSVKRYPVLA KLIEAEDADV VGMQEVERPF AEYLAAQPWC RERYIMSCSP QSPILDPWGV LLLVRRGGRW PLQQLKHLNV PAWSGHVSLM PVATLDLSAG ATHADISRTE GGSSAARSVR RKLTAPQWMV NVCSMHLLAP FVKVNEVART GQDQALRLAL TRQLQGDTVV MGDFNDWPSN EFVMPSESRY VECWPIIHPG DYGKTMDESN TFCKLKIEEI FFGRSDKVFL RTGAATTSSP ARGVLKPVEA HLVGTRSINE ENGNQEAPAY LFPSDHYGVS MQFQVL
NT Sequence
ATGCGGGGCG TGCTAGCGCG TAGCGCATGT CGTCTCACTT CCTTTCAGAA AGGGACGCGG CTAGCGGATG TCTACCAACT CCTCATCACG AAGAAGCCGG TTGAGTATGA CTACGTCGCG ATTGACGTGA ACGCCTTTGT CGGCGGTGCT ATACGCATCA CCAAGAGCCT GTCCACGGAG CAGCGTCGCA ATAAAGAGGC TTCGCGACAT GTCTCTAACT CCATCATGCA GATACTCCGC CGCATTGTGT GCCGTCACTC TCTTCTCCTC GCTTTTGACG GCCCAGACAC GTTTGCGAAG GCGTACAAGC TTCGCACCAC GCTGTCAACA CGGCGACTTG ACATGCAAAC TCAGCGCCTC CCCGGTGGTA CTTTGATGCG TGCGGTGGAG GATCGCATAG TCAAGACAAT GCCGTGGGGG CGCGGGCTGA TTCCTAGCGA GGTGGTTGTT TCTGGTGTCA ACGTGGAGGG CCCGGTGGAG CGAAAGGTGA CGGCGTGGGC TCTTGACTTG GCTTGCCGCG ACGGGGAGCA GCACATCTCC AAGTCTATGT GTCTCATTGG GGCAGGGGAG CTGTGGATGA ATATGCTCGC CCTCACGCCG TTTTTTCAAG GCACAAGCAT CTTACATGGA TCGTCCGACC TGCGCCACAT GACGCTCAGC GACTCCCTGA CGTGGCTCGA GCTCGGCGAC ACTCTGTTGA CTGGCGATGC ATCTGTCATC ACAGCAGCGC GGACCGATGC GCTGCTGCTT TATCTGCTGT GCCATGGCTG CTCCACCACA AACCTCTCGC CGCTGTCTGG CTCGAACTTT GTGGTTCTCA TGGAGACATA CCACACTCGG CGCCGCGAAG CCGCAGTTGC TGCAGACCCG GCAGCTGGGA GACCAACCTT TCAACTCATC CGCGAGCAGC CGAACGGCAC CCTGGTGCTT GACGTACAGG CGCTCTACCT ACTGTTTGTG GCGGAAAAGG ATATGGCAGC AACAACACAA TCGCTGGCGG CGCTCCCCGT GCCGGAAAAA GGTGAGAGTC CACACGCAGC GCCACTGACG ACCTTCTCCG TGCCGGTCTC TTGCTCAGGC GGCAGCTCAG CAGAGGAGCA GATGCGTGAC TCTCGCGTAT CGGACATGTT GTACGGTAGC TATCTTAGCC ACCTGCTGAA CTCTCATCAC GTTTTCTGCC ACGGCGAGAT GCTCGATCGG TTCCGTGTGC CGGCGTACAT GGAGGCGGAA GTGCGAATGG GCAGTATTGG CCCCAGCGGC GCTGCGGGTT CCCGCGCCAG CGTGATTGTC GTGGGGAACT GGATCCGCTT TCTGCTCCAC TGTCTCCAAC GCGGGGGGCG CTATGCTTTT GCAAGTGACA CAGGCGTGGA GCTGAGCGAC AGCATCTCGA AGTGTGCTTC ACACGCTACG AGGGCTTCCA ATGTCAGCGG TGCGTCGTCA TCGAATGACC CCGCTGCCCC CGGCACCACA GCGTACTACC CTTTCTGGTC TTCCACTCAG CCGTTGACGG CAGCCGAGTT CACCATTCTT AGCACCCCGA ATTCGGGTAT CCTTGATCCG CTACTACTGG ATTACGTGCC CGGGGTAACG CCGGCGCTAC GGTCAGAGCT GCCGCGGGCG CTTACGTGTG TGCAGGCAAA CCTCCCTGAG CTTCGCAGAT CACTGCAGCA CTTCTTCGCG TACGCAACGA CTGATCGGCT GCACCCATCT CTGTGCCTGG CGCCGTCTTA CCACTGGATC CAAAACGAAA AGAGCAAGGT GTGGTCCATG CGATACGTGG ACTTGGGTGT GGTCTCACGG CAGCAGGGTG TCCGTCACGC ACGCAGCTTG CTGCCAGGCA TGGGCATAGA GCAGGAAGCG CCGAAGGAAG GCGCCGTGGT ATTTGACGCC GAATTAGAGA TGTGGACCTC GCAGCCACGT CGCTTCGCTG CTTTGTCAGC GTCGGAGCAG ATGAGGGAAC AGCAGGAGCA TACGCATGCC GACGGGCCAT CGTCATCATG TATGGCGGCC ACGGCTGTTT CTTCCGCGGA TGTGGCCGAG GTGCCCGTGC TGAAGGTGCT CACATGGAAC GTCATGTTCG ATCGTTACAG CAACCAACCT ACCCCACTCG GTATGCCAGG CATCGACTGG TGCAGCGTGA AGCGATACCC AGTGCTGGCA AAGCTCATCG AGGCTGAAGA CGCCGATGTG GTGGGGATGC AGGAAGTGGA GCGGCCCTTT GCTGAGTATC TCGCGGCGCA GCCGTGGTGT CGCGAGCGTT ACATCATGTC TTGCAGTCCG CAGAGCCCCA TCTTGGATCC GTGGGGCGTT CTGCTACTTG TGCGCCGCGG CGGGCGGTGG CCACTGCAGC AGCTGAAACA CCTGAATGTG CCAGCATGGT CAGGACACGT GTCCCTCATG CCTGTGGCTA CGCTGGATTT GAGTGCAGGC GCCACCCACG CCGACATCAG TCGCACCGAG GGTGGCAGCT CCGCTGCAAG GAGTGTCAGA AGAAAGCTGA CAGCGCCGCA GTGGATGGTA AACGTCTGTT CCATGCACTT GCTGGCACCA TTTGTAAAGG TGAACGAGGT GGCCCGCACG GGCCAGGATC AAGCTCTGCG CCTTGCTCTG ACGCGGCAGC TTCAAGGTGA CACGGTGGTG ATGGGTGACT TCAACGACTG GCCGAGCAAC GAGTTCGTCA TGCCGTCCGA GTCGAGGTAC GTGGAGTGCT GGCCGATCAT TCATCCCGGC GACTACGGGA AAACTATGGA CGAGTCAAAC ACCTTCTGCA AGCTGAAGAT TGAAGAGATC TTCTTCGGCC GCTCTGACAA GGTGTTCCTC CGGACAGGTG CCGCTACAAC CTCGAGCCCT GCCCGAGGCG TGCTCAAGCC AGTGGAGGCT CATCTAGTAG GAACGCGGAG CATCAATGAA GAAAACGGCA ATCAAGAAGC TCCAGCGTAT CTGTTCCCGT CTGACCACTA CGGAGTCAGC ATGCAGTTCC AAGTCCTGTG A
Details for LetaA.19505.a.B3.GE40877
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV LKVLTWNVMF DRYSNQPTPL GMPGIDWCSV KRYPVLAKLI EAEDADVVGM QEVERPFAEY LAAQPWCRER YIMSCSPQSP ILDPWGVLLL VRRGGRWPLQ QLKHLNVPAW SGHVSLMPVA TLDLSAGATH ADISGTEGGS SAARSVRRKL TAPQWMVNVC SMHLLAPFVK VNEVARTGQD QALRLALTRQ LQGDTVVMGD FNDWPSNEFV MPSESRYVEC WPIIHPGDYG KTMDESNTFC KLKIEEIFFG RSDKVFLRTG AATTSSPARG VLKPVEAHLV GTRSINEENG NQEAPAYLFP SDHYGV
Validated NT Sequence
tgactccgta gtggtcagac gggaacagat acgctggagc ttcttgattg ccgttttctt cattgatgct ccgcgttcct actagatgag cctccactgg cttgagcacg cctcgggcag ggctcgaggt tgtagcggca cctgtccgga ggaacacctt gtcagagcgg ccgaagaaga tctcttcaat cttcagcttg cagaaggtgt ttgactcgtc catagttttc ccgtagtcgc cgggatgaat gatcggccag cactccacgt acctcgactc ggacggcatg acgaactcgt tgctcggcca gtcgttgaag tcacccatca ccaccgtgtc accttgaagc tgccgcgtca gagcaaggcg cagagcttga tcctggcccg tgcgggccac ctcgttcacc tttacaaatg gtgccagcaa gtgcatggaa cagacgttta ccatccactg cggcgctgtc agctttcttc tgacactcct tgcagcggag ctgccaccct cggtgccact gatgtcggcg tgggtggcgc ctgcactcaa atccagcgta gccacaggca tgagggacac gtgtcctgac catgctggca cattcaggtg tttcagctgc tgcagtggcc accgcccgcc gcggcgcaca agtagcagaa cgccccacgg atccaagatg gggctctgcg gactgcaaga catgatgtaa cgctcgcgac accacggctg cgccgcgaga tactcagcaa agggccgctc cacttcctgc atccccacca catcggcgtc ttcagcctcg atgagctttg ccagcactgg gtatcgcttc acgctgcacc agtcgatgcc tggcataccg agtggggtag gttggttgct gtaacgatcg aacatgacgt tccatgtgag caccttcagc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHVL KVLTWNVMFD RYSNQPTPLG MPGIDWCSVK RYPVLAKLIE AEDADVVGMQ EVERPFAEYL AAQPWCRERY IMSCSPQSPI LDPWGVLLLV RRGGRWPLQQ LKHLNVPAWS GHVSLMPVAT LDLSAGATHA DISRTEGGSS AARSVRRKLT APQWMVNVCS MHLLAPFVKV NEVARTGQDQ ALRLALTRQL QGDTVVMGDF NDWPSNEFVM PSESRYVECW PIIHPGDYGK TMDESNTFCK LKIEEIFFGR SDKVFLRTGA ATTSSPARGV LKPVEAHLVG TRSINEENGN QEAPAYLFPS DHYGVSMQFQ VL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctga aggtgctcac atggaacgtc atgttcgatc gttacagcaa ccaacctacc ccactcggta tgccaggcat cgactggtgc agcgtgaagc gatacccagt gctggcaaag ctcatcgagg ctgaagacgc cgatgtggtg gggatgcagg aagtggagcg gccctttgct gagtatctcg cggcgcagcc gtggtgtcgc gagcgttaca tcatgtcttg cagtccgcag agccccatct tggatccgtg gggcgttctg ctacttgtgc gccgcggcgg gcggtggcca ctgcagcagc tgaaacacct gaatgtgcca gcatggtcag gacacgtgtc cctcatgcct gtggctacgc tggatttgag tgcaggcgcc acccacgccg acatcagtcg caccgagggt ggcagctccg ctgcaaggag tgtcagaaga aagctgacag cgccgcagtg gatggtaaac gtctgttcca tgcacttgct ggcaccattt gtaaaggtga acgaggtggc ccgcacgggc caggatcaag ctctgcgcct tgctctgacg cggcagcttc aaggtgacac ggtggtgatg ggtgacttca acgactggcc gagcaacgag ttcgtcatgc cgtccgagtc gaggtacgtg gagtgctggc cgatcattca tcccggcgac tacgggaaaa ctatggacga gtcaaacacc ttctgcaagc tgaagattga agagatcttc ttcggccgct ctgacaaggt gttcctccgg acaggtgccg ctacaacctc gagccctgcc cgaggcgtgc tcaagccagt ggaggctcat ctagtaggaa cgcggagcat caatgaagaa aacggcaatc aagaagctcc agcgtatctg ttcccgtctg accactacgg agtcagcatg cagttccaag tcctgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LetaA.19505.a.B3.PS38256
PURIFICATION DATe: 4/10/2017
CONCENTRATION: 24.3mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LKVLTWNVMF DRYSNQPTPL GMPGIDWCSV KRYPVLAKLI EAEDADVVGM QEVERPFAEY LAAQPWCRER YIMSCSPQSP ILDPWGVLLL VRRGGRWPLQ QLKHLNVPAW SGHVSLMPVA TLDLSAGATH ADISGTEGGS SAARSVRRKL TAPQWMVNVC SMHLLAPFVK VNEVARTGQD QALRLALTRQ LQGDTVVMGD FNDWPSNEFV MPSESRYVEC WPIIHPGDYG KTMDESNTFC KLKIEEIFFG RSDKVFLRTG AATTSSPARG VLKPVEAHLV GTRSINEENG NQEAPAYLFP SDHYGV
Validated NT Sequence
tgactccgta gtggtcagac gggaacagat acgctggagc ttcttgattg ccgttttctt cattgatgct ccgcgttcct actagatgag cctccactgg cttgagcacg cctcgggcag ggctcgaggt tgtagcggca cctgtccgga ggaacacctt gtcagagcgg ccgaagaaga tctcttcaat cttcagcttg cagaaggtgt ttgactcgtc catagttttc ccgtagtcgc cgggatgaat gatcggccag cactccacgt acctcgactc ggacggcatg acgaactcgt tgctcggcca gtcgttgaag tcacccatca ccaccgtgtc accttgaagc tgccgcgtca gagcaaggcg cagagcttga tcctggcccg tgcgggccac ctcgttcacc tttacaaatg gtgccagcaa gtgcatggaa cagacgttta ccatccactg cggcgctgtc agctttcttc tgacactcct tgcagcggag ctgccaccct cggtgccact gatgtcggcg tgggtggcgc ctgcactcaa atccagcgta gccacaggca tgagggacac gtgtcctgac catgctggca cattcaggtg tttcagctgc tgcagtggcc accgcccgcc gcggcgcaca agtagcagaa cgccccacgg atccaagatg gggctctgcg gactgcaaga catgatgtaa cgctcgcgac accacggctg cgccgcgaga tactcagcaa agggccgctc cacttcctgc atccccacca catcggcgtc ttcagcctcg atgagctttg ccagcactgg gtatcgcttc acgctgcacc agtcgatgcc tggcataccg agtggggtag gttggttgct gtaacgatcg aacatgacgt tccatgtgag caccttcagc accatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHVL KVLTWNVMFD RYSNQPTPLG MPGIDWCSVK RYPVLAKLIE AEDADVVGMQ EVERPFAEYL AAQPWCRERY IMSCSPQSPI LDPWGVLLLV RRGGRWPLQQ LKHLNVPAWS GHVSLMPVAT LDLSAGATHA DISRTEGGSS AARSVRRKLT APQWMVNVCS MHLLAPFVKV NEVARTGQDQ ALRLALTRQL QGDTVVMGDF NDWPSNEFVM PSESRYVECW PIIHPGDYGK TMDESNTFCK LKIEEIFFGR SDKVFLRTGA ATTSSPARGV LKPVEAHLVG TRSINEENGN QEAPAYLFPS DHYGVSMQFQ VL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTGA AGGTGCTCAC ATGGAACGTC ATGTTCGATC GTTACAGCAA CCAACCTACC CCACTCGGTA TGCCAGGCAT CGACTGGTGC AGCGTGAAGC GATACCCAGT GCTGGCAAAG CTCATCGAGG CTGAAGACGC CGATGTGGTG GGGATGCAGG AAGTGGAGCG GCCCTTTGCT GAGTATCTCG CGGCGCAGCC GTGGTGTCGC GAGCGTTACA TCATGTCTTG CAGTCCGCAG AGCCCCATCT TGGATCCGTG GGGCGTTCTG CTACTTGTGC GCCGCGGCGG GCGGTGGCCA CTGCAGCAGC TGAAACACCT GAATGTGCCA GCATGGTCAG GACACGTGTC CCTCATGCCT GTGGCTACGC TGGATTTGAG TGCAGGCGCC ACCCACGCCG ACATCAGTCG CACCGAGGGT GGCAGCTCCG CTGCAAGGAG TGTCAGAAGA AAGCTGACAG CGCCGCAGTG GATGGTAAAC GTCTGTTCCA TGCACTTGCT GGCACCATTT GTAAAGGTGA ACGAGGTGGC CCGCACGGGC CAGGATCAAG CTCTGCGCCT TGCTCTGACG CGGCAGCTTC AAGGTGACAC GGTGGTGATG GGTGACTTCA ACGACTGGCC GAGCAACGAG TTCGTCATGC CGTCCGAGTC GAGGTACGTG GAGTGCTGGC CGATCATTCA TCCCGGCGAC TACGGGAAAA CTATGGACGA GTCAAACACC TTCTGCAAGC TGAAGATTGA AGAGATCTTC TTCGGCCGCT CTGACAAGGT GTTCCTCCGG ACAGGTGCCG CTACAACCTC GAGCCCTGCC CGAGGCGTGC TCAAGCCAGT GGAGGCTCAT CTAGTAGGAA CGCGGAGCAT CAATGAAGAA AACGGCAATC AAGAAGCTCC AGCGTATCTG TTCCCGTCTG ACCACTACGG AGTCAGCATG CAGTTCCAAG TCCTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT