SSGCID: Target Page

LetaA.19504.a

KREPB8

CENTER ID:	LetaA.19504.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19504.a.B1.GE40705	full length		1	387
LetaA.19504.a.B2.GE40706	ordered domain		58	387

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LtaP16.0330

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHPTRLCGAV RSVMPSRKVS SLHQFLRENA VQSVLDAEDS TTGVSEPLSS LSPKGSSTEG RTLLSSLPVG VFEGLNRHIW VDTKRSFCHL CQEPVGNATI HSSDRDHTSM LFFLLLYTAY PRHDLLTDAA RAPTAGERAA AMVKDGVCAS KPSLPAECNR AAGVPQTISC SRSKLGPLFT PRQVLSGAKQ HFPSLFRYVT DCRTTDRLHV VDDAVRRAEL EALLLYLAHP PHQALSHVLQ GQSPNGFWYN GERMWKAHIS RIVAQLYPPM SAGMMTNFTQ KCWGRSNGER LYDALCLHRI KAYYGWGPFE SKERKAFFLR HLIWELLCVE VRDEVSEEAK LLTSLALRRL AFEMVFLQSM DYMDRVQRVY ELLGSPSLEE LKSLKAL

NT Sequence

ATGCACCCCA CGCGTCTGTG CGGTGCGGTA CGCTCGGTGA TGCCGTCCCG AAAGGTCTCG TCGCTGCACC AGTTTCTTCG CGAAAATGCA GTGCAGAGCG TACTAGATGC GGAGGACTCT ACTACAGGGG TCAGTGAACC CCTGAGCAGC TTGTCGCCAA AGGGGTCCTC CACGGAGGGC CGCACGTTGC TCTCATCGCT ACCAGTGGGC GTGTTTGAAG GCCTGAATCG ACATATTTGG GTGGATACCA AGCGCAGCTT TTGCCACCTT TGCCAGGAAC CGGTGGGTAA CGCCACCATC CATAGCAGCG ACCGTGATCA CACCAGCATG CTGTTTTTTC TGCTTCTCTA CACTGCGTAC CCCAGGCACG ACTTGCTCAC GGACGCGGCG CGAGCCCCGA CAGCAGGAGA GAGAGCAGCG GCCATGGTGA AAGATGGCGT CTGCGCCTCA AAACCGTCTC TGCCGGCCGA GTGCAACAGA GCCGCAGGTG TGCCTCAGAC GATATCCTGC TCGAGAAGCA AACTCGGCCC GCTGTTCACC CCTAGGCAGG TCCTCAGCGG GGCGAAGCAG CATTTTCCAT CGCTGTTTCG CTACGTCACG GACTGTCGCA CTACAGACCG CCTACATGTA GTCGACGATG CTGTGCGCCG TGCAGAGCTA GAAGCGCTCT TGCTCTACCT CGCACATCCA CCACACCAGG CGCTCTCACA CGTTCTGCAA GGCCAAAGCC CGAATGGCTT CTGGTACAAT GGGGAGCGCA TGTGGAAGGC GCATATTAGT CGCATCGTTG CGCAGCTTTA TCCACCCATG TCCGCTGGTA TGATGACCAA CTTCACCCAG AAGTGCTGGG GTCGCAGTAA CGGGGAGAGG CTGTACGACG CACTCTGCCT GCACCGCATC AAGGCCTACT ATGGCTGGGG CCCATTCGAG AGCAAGGAGC GCAAGGCCTT CTTTCTAAGG CATCTGATCT GGGAGCTGCT CTGCGTTGAA GTCCGCGACG AGGTGTCGGA GGAGGCGAAG CTGTTGACGT CGCTTGCGCT TCGACGGCTC GCGTTCGAGA TGGTATTCCT GCAGTCGATG GACTACATGG ACCGTGTACA GCGGGTGTAC GAACTGCTGG GAAGCCCCTC GCTGGAGGAG CTCAAAAGCT TGAAGGCGCT GTGA

Details for LetaA.19504.a.B1.GE40705

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMH PTRLCGAVRS VMPSRKVSSL HQFLRENAVQ SVLDAEDSTT GVSEPLSSLS PKGSSTEGRT LLSSLPVGVF EGLNRHIWVD TKRSFCHLCQ EPVGNATIHS SDRDHTSMLF FLLLYTAYPR HDLLTDAARA PTAGERAAAM VKDGVCASKP SLPAECNRAA GVPQTISCSR SKLGPLFTPR QVLSGAKQHF PSLFRYVTDC RTTDRLHVVD DAVRRAELEA LLLYLAHPPH QALSHVLQGQ SPNGFWYNGE RMWKAHISRI VAQLYPPMSA GMMTNFTQKC WGRSNGERLY DALCLHRIKA YYGWGPFESK ERKAFFLRHL IWELLCVEVR DEVSEEAKLL TSLALRRLAF EMVFLQSMDY MDRVQRVYEL LGSPSLE

Validated NT Sequence

atggctcacc accaccacca ccatatgcac cccacgcgtc tgtgcggtgc ggtacgctcg gtgatgccgt cccgaaaggt ctcgtcgctg caccagtttc ttcgcgaaaa tgcagtgcag agcgtactag atgcggagga ctctactaca ggggtcagtg aacccctgag cagcttgtcg ccaaaggggt cctccacgga gggccgcacg ttgctctcat cgctaccagt gggcgtgttt gaaggcctga atcgacatat ttgggtggat accaagcgca gcttttgcca cctttgccag gaaccggtgg gtaacgccac catccatagc agcgaccgtg atcacaccag catgctgttt tttctgcttc tctacactgc gtaccccagg cacgacttgc tcacggacgc ggcgcgagcc ccgacagcag gagagagagc agcggccatg gtgaaagatg gcgtctgcgc ctcaaaaccg tctctgccgg ccgagtgcaa cagagccgca ggtgtgcctc agacgatatc ctgctcgaga agcaaactcg gcccgctgtt cacccctagg caggtcctca gcggggcgaa gcagcatttt ccatcgctgt ttcgctacgt cacggactgt cgcactacag accgcctaca tgtagtcgac gatgctgtgc gccgtgcaga gctagaagcg ctcttgctct acctcgcaca tccaccacac caggcgctct cacacgttct gcaaggccaa agcccgaatg gcttctggta caatggggag cgcatgtgga aggcgcatat tagtcgcatc gttgcgcagc tttatccacc catgtccgct ggtatgatga ccaacttcac ccagaagtgc tggggtcgca gtaacgggga gaggctgtac gacgcactct gcctgcaccg catcaaggcc tactatggct ggggcccatt cgagagcaag gagcgcaagg ccttctttct aaggcatctg atctgggagc tgctctgcgt tgaagtccgc gacgaggtgt cggaggaggc gaagctgttg acgtcgcttg cgcttcgacg gctcgcgttc gagatggtat tcctgcagtc gatggactac atggaccgtg tacagcgggt gtacgaactg ctgggaagcc cctcgctgga gga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacc ccacgcgtct gtgcggtgcg gtacgctcgg tgatgccgtc ccgaaaggtc tcgtcgctgc accagtttct tcgcgaaaat gcagtgcaga gcgtactaga tgcggaggac tctactacag gggtcagtga acccctgagc agcttgtcgc caaaggggtc ctccacggag ggccgcacgt tgctctcatc gctaccagtg ggcgtgtttg aaggcctgaa tcgacatatt tgggtggata ccaagcgcag cttttgccac ctttgccagg aaccggtggg taacgccacc atccatagca gcgaccgtga tcacaccagc atgctgtttt ttctgcttct ctacactgcg taccccaggc acgacttgct cacggacgcg gcgcgagccc cgacagcagg agagagagca gcggccatgg tgaaagatgg cgtctgcgcc tcaaaaccgt ctctgccggc cgagtgcaac agagccgcag gtgtgcctca gacgatatcc tgctcgagaa gcaaactcgg cccgctgttc acccctaggc aggtcctcag cggggcgaag cagcattttc catcgctgtt tcgctacgtc acggactgtc gcactacaga ccgcctacat gtagtcgacg atgctgtgcg ccgtgcagag ctagaagcgc tcttgctcta cctcgcacat ccaccacacc aggcgctctc acacgttctg caaggccaaa gcccgaatgg cttctggtac aatggggagc gcatgtggaa ggcgcatatt agtcgcatcg ttgcgcagct ttatccaccc atgtccgctg gtatgatgac caacttcacc cagaagtgct ggggtcgcag taacggggag aggctgtacg acgcactctg cctgcaccgc atcaaggcct actatggctg gggcccattc gagagcaagg agcgcaaggc cttctttcta aggcatctga tctgggagct gctctgcgtt gaagtccgcg acgaggtgtc ggaggaggcg aagctgttga cgtcgcttgc gcttcgacgg ctcgcgttcg agatggtatt cctgcagtcg atggactaca tggaccgtgt acagcgggtg tacgaactgc tgggaagccc ctcgctggag gagctcaaaa gcttgaaggc gctgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LetaA.19504.a.B2.GE40706

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT EGRTLLSSLP VGVFEGLNRH IWVDTKRSFC HLCQEPVGNA TIHSSDRDHT SMLFFLLLYT AYPRHDLLTD AARAPTAGER AAAMVKDGVC ASKPSLPAEC NRAAGVPQTI SCSRSKLGPL FTPRQVLSGA KQHFPSLFRY VTDCRTTDRL HVVDDAVRRA ELEALLLYLA HPPHQALSHV LQGQSPNGFW YNGERMWKAH ISRIVAQLYP PMSAGMMTNF TQKCWGRSNG ERLYDALCLH RIKAYYGWGP FESKERKAFF LRHLIWELLC VEVRDEVSEE AKLLTSLALR RLAFEMVFLQ SMDYMDRVQR VYELLGSPSL E

Validated NT Sequence

atggctcacc accaccacca ccatatgacg gagggccgca cgttgctctc atcgctacca gtgggcgtgt ttgaaggcct gaatcgacat atttgggtgg ataccaagcg cagcttttgc cacctttgcc aggaaccggt gggtaacgcc accatccata gcagcgaccg tgatcacacc agcatgctgt tttttctgct tctctacact gcgtacccca ggcacgactt gctcacggac gcggcgcgag ccccgacagc aggagagaga gcagcggcca tggtgaaaga tggcgtctgc gcctcaaaac cgtctctgcc ggccgagtgc aacagagccg caggtgtgcc tcagacgata tcctgctcga gaagcaaact cggcccgctg ttcaccccta ggcaggtcct cagcggggcg aagcagcatt ttccatcgct gtttcgctac gtcacggact gtcgcactac agaccgccta catgtagtcg acgatgctgt gcgccgtgca gagctagaag cgctcttgct ctacctcgca catccaccac accaggcgct ctcacacgtt ctgcaaggcc aaagcccgaa tggcttctgg tacaatgggg agcgcatgtg gaaggcgcat attagtcgca tcgttgcgca gctttatcca cccatgtccg ctggtatgat gaccaacttc acccagaagt gctggggtcg cagtaacggg gagaggctgt acgacgcact ctgcctgcac cgcatcaagg cctactatgg ctggggccca ttcgagagca aggagcgcaa ggccttcttt ctaaggcatc tgatctggga gctgctctgc gttgaagtcc gcgacgaggt gtcggaggag gcgaagctgt tgacgtcgct tgcgcttcga cggctcgcgt tcgagatggt attcctgcag tcgatggact acatggaccg tgtacagcgg gtgtacgaac tgctgggaag cccctcgctg gaggag

Expected Protein Sequence

MAHHHHHHTE GRTLLSSLPV GVFEGLNRHI WVDTKRSFCH LCQEPVGNAT IHSSDRDHTS MLFFLLLYTA YPRHDLLTDA ARAPTAGERA AAMVKDGVCA SKPSLPAECN RAAGVPQTIS CSRSKLGPLF TPRQVLSGAK QHFPSLFRYV TDCRTTDRLH VVDDAVRRAE LEALLLYLAH PPHQALSHVL QGQSPNGFWY NGERMWKAHI SRIVAQLYPP MSAGMMTNFT QKCWGRSNGE RLYDALCLHR IKAYYGWGPF ESKERKAFFL RHLIWELLCV EVRDEVSEEA KLLTSLALRR LAFEMVFLQS MDYMDRVQRV YELLGSPSLE ELKSLKAL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacggagg gccgcacgtt gctctcatcg ctaccagtgg gcgtgtttga aggcctgaat cgacatattt gggtggatac caagcgcagc ttttgccacc tttgccagga accggtgggt aacgccacca tccatagcag cgaccgtgat cacaccagca tgctgttttt tctgcttctc tacactgcgt accccaggca cgacttgctc acggacgcgg cgcgagcccc gacagcagga gagagagcag cggccatggt gaaagatggc gtctgcgcct caaaaccgtc tctgccggcc gagtgcaaca gagccgcagg tgtgcctcag acgatatcct gctcgagaag caaactcggc ccgctgttca cccctaggca ggtcctcagc ggggcgaagc agcattttcc atcgctgttt cgctacgtca cggactgtcg cactacagac cgcctacatg tagtcgacga tgctgtgcgc cgtgcagagc tagaagcgct cttgctctac ctcgcacatc caccacacca ggcgctctca cacgttctgc aaggccaaag cccgaatggc ttctggtaca atggggagcg catgtggaag gcgcatatta gtcgcatcgt tgcgcagctt tatccaccca tgtccgctgg tatgatgacc aacttcaccc agaagtgctg gggtcgcagt aacggggaga ggctgtacga cgcactctgc ctgcaccgca tcaaggccta ctatggctgg ggcccattcg agagcaagga gcgcaaggcc ttctttctaa ggcatctgat ctgggagctg ctctgcgttg aagtccgcga cgaggtgtcg gaggaggcga agctgttgac gtcgcttgcg cttcgacggc tcgcgttcga gatggtattc ctgcagtcga tggactacat ggaccgtgta cagcgggtgt acgaactgct gggaagcccc tcgctggagg agctcaaaag cttgaaggcg ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat