SSGCID: Target Page

LetaA.19503.a

KREPB7

CENTER ID:	LetaA.19503.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19503.a.B2.GE40876	RNase III-like catalytic-like domain		126	481

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LtaP15.1180

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTLSPIRRG AAAVRTRMLR AASSSMSSRS RVTMANGAAD QGTNVLRPPY PTEGGSVPVY SMMHGQIDKR NWVFRNPQWC ELCNEPVSRW LDHQSRKDHA LLDLHYSQLA EWPRRWNPEE VLATFMDHLG IESVEPYHAL FSKLDSEGRT KLYAMLVTLE EAGMLHFGSS RDTYLSRMAG GMRGLDHQGA LVLHECLMGP FLRLFPNGLI QDYSNLLDFI SCSYNMETVY DMCGMYTLDK VALRERYGPS SPAAMGLGGV ATCSSASRGY ENDLFASLSA TSSSSSAAFD TAADAVSAPG VSSGTTTRLA QLQAARAAKL QEKSEEEAFS RKTTFVRQLL GQLRWLLMPE QEHPAGFTFP PHIITLGELC LKGLVVAIIE TRLCEYMVRA EPVWVSFGLE RRRLKQSAIA NADDVVPQPV QYSYRPMARD LCDLYSTQPS ALDAMVQAGL LKKKLPTLKA FGKYEASRAH AQPVKREAAI EKSSRGSPSS ASAPPAASRP LQPPILRKAP QGKEAQGHEI ARRGAGGGVL R

NT Sequence

ATGCTAACAT TGTCGCCCAT TCGCCGCGGC GCCGCCGCCG TGAGGACGCG GATGTTACGG GCCGCTTCTA GCAGCATGTC TTCCAGGTCA CGTGTCACGA TGGCCAACGG CGCGGCAGAC CAGGGCACGA ATGTCTTACG GCCTCCGTAT CCGACGGAGG GTGGTAGCGT TCCAGTATAC TCGATGATGC ACGGGCAGAT TGACAAGCGC AACTGGGTAT TTCGCAACCC ACAATGGTGC GAGCTGTGCA ACGAGCCGGT GTCGCGTTGG TTAGACCATC AGAGTCGCAA GGACCACGCT TTATTAGATT TGCACTACTC GCAGTTGGCG GAGTGGCCGC GTCGATGGAA CCCCGAGGAG GTCCTGGCGA CCTTCATGGA TCACCTCGGC ATCGAGAGCG TGGAGCCGTA CCACGCTCTC TTCTCCAAGC TGGATAGTGA GGGTCGTACA AAGCTGTACG CAATGCTCGT CACACTGGAA GAGGCTGGCA TGCTGCACTT TGGCAGCAGC CGTGACACCT ACCTTAGCCG CATGGCTGGT GGGATGCGCG GACTCGACCA TCAAGGCGCG CTGGTGCTGC ACGAGTGCCT GATGGGGCCC TTTCTTCGAC TCTTTCCAAA CGGCCTTATA CAGGATTACT CGAACCTCCT CGACTTCATT TCCTGCAGCT ACAACATGGA GACGGTGTAT GACATGTGCG GCATGTACAC CCTGGACAAG GTGGCACTGC GGGAGCGCTA CGGGCCCAGC TCCCCCGCCG CAATGGGCCT GGGCGGCGTC GCGACGTGCT CCTCCGCGTC TCGTGGCTAC GAAAATGACT TGTTTGCATC CCTGTCGGCG ACCTCATCGT CGTCGTCCGC TGCCTTCGAC ACCGCGGCCG ACGCTGTCAG CGCCCCAGGT GTCAGCAGCG GTACCACGAC TCGGCTCGCG CAGCTGCAGG CCGCCCGCGC CGCAAAGTTG CAGGAGAAGT CAGAGGAGGA GGCCTTCTCG CGCAAGACAA CATTTGTGCG TCAACTCCTC GGGCAGCTGC GGTGGCTGCT GATGCCGGAG CAGGAACACC CGGCCGGCTT CACGTTCCCA CCGCACATCA TCACCCTGGG CGAGCTGTGT CTGAAGGGGC TGGTGGTCGC CATTATTGAA ACGCGCCTCT GCGAGTACAT GGTACGTGCC GAGCCTGTCT GGGTTTCCTT CGGACTCGAG CGGCGGCGGC TGAAGCAGAG CGCGATCGCG AATGCGGACG ACGTGGTGCC ACAGCCTGTG CAGTATTCCT ACCGCCCCAT GGCCCGGGAC CTGTGCGACC TGTACTCCAC CCAGCCAAGC GCACTTGACG CAATGGTGCA GGCGGGCCTA CTGAAGAAGA AGCTGCCGAC GCTAAAAGCG TTTGGGAAGT ACGAGGCATC ACGGGCACAT GCACAACCAG TGAAAAGAGA AGCCGCGATA GAGAAGAGCT CACGTGGAAG TCCCTCATCT GCATCCGCAC CCCCTGCGGC GAGCAGGCCA CTGCAACCCC CCATTCTTCG CAAGGCACCT CAGGGTAAGG AAGCGCAAGG GCACGAGATA GCGCGTCGAG GCGCGGGGGG AGGCGTGCTG CGGTAG

Details for LetaA.19503.a.B2.GE40876

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD HLGIESVEPY HALFSKLDSE GRTKLYAMLV TLEEAGMLHF GSSRDTYLSR MAGGMRGLDH QGALVLHECL MGPFLRLFPN GLIQDYSNLL DFISCSYNME TVYDMCGMYT LDKVALRERY GPSSPAAMGL GGVATCSSAS RGYENDLFAS LSATSSSSSA AFDTAADAVS APGVSSGTTT RLAQLQAARA AKLQEKSEEE AFSRKTTFVR QLLGQLRWLL MPEQEHPAGF TFPPHIITLG ELCLKGLVVA IIETRLCEYM VRAEPVWVSF GLERRRLKQS AIANADDVVP QPVQYSYRPM ARDLCDLYST QPSALDAMVQ AGLLKKKLPT LKAFGKYEAS RAHAQP

Validated NT Sequence

cactggttgt gcatgtgccc gtgatgcctc gtacttccca aacgctttta gcgtcggcag cttcttcttc agtaggcccg cctgcaccat tgcgtcaagt gcgcttggct gggtggagta caggtcgcac aggtcccggg ccatggggcg gtaggaatac tgcacaggct gtggcaccac gtcgtccgca ttcgcgatcg cgctctgctt cagccgccgc cgctcgagtc cgaaggaaac ccagacaggc tcggcacgta ccatgtactc gcagaggcgc gtttcaataa tggcgaccac cagccccttc agacacagct cgcccagggt gatgatgtgc ggtgggaacg tgaagccggc cgggtgttcc tgctccggca tcagcagcca ccgcagctgc ccgaggagtt gacgcacaaa tgttgtcttg cgcgagaagg cctcctcctc tgacttctcc tgcaactttg cggcgcgggc ggcctgcagc tgcgcgagcc gagtcgtggt accgctgctg acacctgggg cgctgacagc gtcggccgcg gtgtcgaagg cagcggacga cgacgatgag gtcgccgaca gggatgcaaa caagtcattt tcgtagccac gagacgcgga ggagcacgtc gcgacgccgc ccaggcccat tgcggcgggg gagctgggcc cgtagcgctc ccgcagtgcc accttgtcca gggtgtacat gccgcacatg tcatacaccg tctccatgtt gtagctgcag gaaatgaagt cgaggaggtt cgagtaatcc tgtataaggc cgtttggaaa gagtcgaaga aagggcccca tcaggcactc gtgcagcacc agcgcgcctt gatggtcgag tccgcgcatc ccaccagcca tgcggctaag gtaggtgtca cggctgctgc caaagtgcag catgccagcc tcttccagtg tgacgagcat tgcgtacagc tttgtacgac cctcactatc cagcttggag aagagagcgt ggtacggctc cacgctctcg atgccgaggt gatccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc acctcggcat cgagagcgtg gagccgtacc acgctctctt ctccaagctg gatagtgagg gtcgtacaaa gctgtacgca atgctcgtca cactggaaga ggctggcatg ctgcactttg gcagcagccg tgacacctac cttagccgca tggctggtgg gatgcgcgga ctcgaccatc aaggcgcgct ggtgctgcac gagtgcctga tggggccctt tcttcgactc tttccaaacg gccttataca ggattactcg aacctcctcg acttcatttc ctgcagctac aacatggaga cggtgtatga catgtgcggc atgtacaccc tggacaaggt ggcactgcgg gagcgctacg ggcccagctc ccccgccgca atgggcctgg gcggcgtcgc gacgtgctcc tccgcgtctc gtggctacga aaatgacttg tttgcatccc tgtcggcgac ctcatcgtcg tcgtccgctg ccttcgacac cgcggccgac gctgtcagcg ccccaggtgt cagcagcggt accacgactc ggctcgcgca gctgcaggcc gcccgcgccg caaagttgca ggagaagtca gaggaggagg ccttctcgcg caagacaaca tttgtgcgtc aactcctcgg gcagctgcgg tggctgctga tgccggagca ggaacacccg gccggcttca cgttcccacc gcacatcatc accctgggcg agctgtgtct gaaggggctg gtggtcgcca ttattgaaac gcgcctctgc gagtacatgg tacgtgccga gcctgtctgg gtttccttcg gactcgagcg gcggcggctg aagcagagcg cgatcgcgaa tgcggacgac gtggtgccac agcctgtgca gtattcctac cgccccatgg cccgggacct gtgcgacctg tactccaccc agccaagcgc acttgacgca atggtgcagg cgggcctact gaagaagaag ctgccgacgc taaaagcgtt tgggaagtac gaggcatcac gggcacatgc acaaccagtg aaaagagaag ccgcgataga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat