CENTER ID: | LetaA.19501.a |
ORGANISM: | Leishmania tarentolae Parrot-TarII |
ASSOCIATED DISEASE: | |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
unclassified |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LetaA.19501.a.B1.GE40874 | full length | 1 | 663 | ||
LetaA.19501.a.B2.GE40707 | C-term domain | 384 | 657 | ||
LetaA.19501.a.B3.GE40708 | N-extended J-binding domain | 83 | 284 | ||
LetaA.19501.a.B4.GE40709 | J-binding domain | 117 | 278 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LetaA.19501.a.B3.PS38165 | N-extended J-binding domain | 83 | 284 |
METHOD | RESULTS |
---|---|
comparative modelling | Robetta_79389 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | TriTrypDB:LtaP36.0380 |
HARVESTED ON: | 12/5/2016 |
SEQUENCED ON: | 12/6/2016 |
EXPECTED MW: | 75kDa |
OBSERVED MW: | 75kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 33kDa |
OBSERVED MW: | 33kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 25kDa |
OBSERVED MW: | 25kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 20kDa |
OBSERVED MW: | 20kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 1/11/2017 |
CONCENTRATION: | 51mg/ml |
OBSERVED MW: | 25kDa |
EXPRESSION LEVEL: | High Expression |
PROTEIN PURIFICATION BUFFER: | SEC |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 20 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |