LetaA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID: LetaA.19501.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LetaA.19501.a.B1.GE40874 full length 1 663
LetaA.19501.a.B2.GE40707 C-term domain 384 657
LetaA.19501.a.B3.GE40708 N-extended J-binding domain 83 284
LetaA.19501.a.B4.GE40709 J-binding domain 117 278
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LetaA.19501.a.B3.PS38165 N-extended J-binding domain 83 284
Molecular models
METHOD RESULTS
comparative modelling Robetta_79389
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP36.0380
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSKQTEGTT ADEERQRTSV SSSDSSSVST TDSDEVSSGA SDSSSSGSGS TSASESSDRE RPSSLSQRQN TATRERPTST QQLQQQRHVQ LNPELRFERP DKEYIPLPPL RDMQDMSKVL FLLSTDKKRY PDGRHRTLDY FRASVEMFVT EVRQEYKRQY QQAQRGGRAM QRFTWKNSGE LAICFACCCD NVKLLYDSLQ PGPLKPLWDA FVSQLAPMLI IQSRVPEMML SSQTYHTKYM DWVKGGTRYG GEQSTANVRF PSVADRRVKV ETYLRSGAGY KIEAVQVGLT AAVPAAAPVA AGSHRSSTAA SAPVQATETR APFAGRLPGT GFAKVPVSPG IGGPRPPLKV PMKHAGGGAA QGSVTGAAAP PLASTCVKQM PPEMKHLFWL SVLAHVKGGD ASRAFPDDRL EVLPGLLRLC EMTEHTEEMC ILMDVLAASS SGVQTVFERL GGLLSFRRFA SHFIQREEAE PLMQIIEQLA RWRVVAWGST SQQAWQTDMN QRRTRDLMWL RTLPLIPEEK RAAWGALLLV MEQRYIFKAP RPDDTTTMDN PLKRRRPEIE LGDLHAVLSD NVDMQKWVVP RVNVFSAVRL PVELRVPPLC TKSEEDTVRF LNSKREYAEK AQVAWRERLE KILCSQPHAL DSLEPVPLWY DPYALLSLAS SQT
NT Sequence
atgtcctcaa aacaaactga gggcacaact gctgacgagg agcgccagcg aacttcggtc tcgtctagcg actcgtcgag tgtcagcact acagactctg acgaagtcag cagcggtgcc tctgacagca gcagcagcgg cagcggcagc acctcggcct ccgaatcatc tgatagagag cggccctcgt ctctctcaca gcgtcaaaac accgctacaa gagagaggcc gacgtccaca cagcagctac agcagcagcg gcatgtgcag ctgaatcccg agctgcgctt cgagcggcca gacaaggagt acatcccact gccaccccta agagacatgc aggacatgtc caaggttctg tttctcttga gcaccgataa gaaacgctac ccggatggtc gccaccgcac gctggactac ttccgtgcct cggtggagat gtttgtgacg gaggttcggc aggagtacaa acggcagtac cagcaggcgc agcgcggtgg ccgtgcaatg cagcggttca cctggaagaa ctcgggcgag ctagctattt gctttgcctg ctgctgcgac aacgtgaagc ttctgtatga cagcctccaa cctggcccac tcaagccact ctgggacgcc tttgtcagcc agctcgcgcc gatgctcatc attcagagtc gcgtgccgga aatgatgctg tcatctcaaa cttaccacac caagtatatg gactgggtaa agggtggcac acgctacgga ggagagcagt cgactgcgaa tgtccgcttc ccctccgtgg cagaccgtcg cgtgaaggtt gaaacatacc tacggagcgg tgcagggtac aagatcgagg ctgtgcaggt ggggttgacg gccgccgtgc ccgcggctgc tccagtcgcg gcggggtctc accgttcttc gactgctgct tcggctccgg tgcaggctac tgagactcgg gcgcctttcg cggggcggct gcccggcaca ggttttgcaa aggtgccggt ctctcccggc atcggtggac ctcgcccgcc actgaaggtg cctatgaagc acgctggcgg cggtgctgca cagggcagtg tgacgggggc cgccgcgcct ccgcttgcct ccacctgtgt gaagcagatg cccccggaga tgaagcattt gttttggctg tcggttcttg cgcacgtaaa gggcggggat gcgtcccggg cgtttccgga cgaccgactg gaggtgctcc cgggcctgct gcggctgtgc gagatgacgg agcacacgga ggagatgtgc attttgatgg acgtactggc ggcgtccagc agtggcgtcc agaccgtctt tgaaagactt ggcggtctgc tgtcattccg ccggttcgcc tcccacttta tccagcgtga ggaggcagag ccactcatgc aaatcatcga gcagctcgcg cggtggcgcg ttgtggcatg gggcagtacc tcacagcagg catggcagac cgatatgaac caacggcgca cacgtgatct gatgtggctt cgtactctgc ccctcatccc ggaggagaag cgggcagcgt ggggtgcgct actgctggtg atggagcaac gttacatttt caaggctccg cgccctgatg acacgaccac catggataat ccactgaagc gtcggcgacc cgagatcgag ctcggcgacc tgcatgctgt gttgagtgac aacgtcgaca tgcaaaaatg ggtggttccc cgagtcaatg tgttctccgc tgtccgccta ccggtggagc tgcgcgtgcc accactgtgt acaaagtctg aggaggatac cgtgcgcttt ctcaactcta agcgcgagta tgccgaaaag gcgcaggtgg cgtggcgtga gagactagag aagattttgt gctcgcagcc tcatgcgttg gattcccttg aaccggtgcc tctctggtat gacccgtacg cgctgctgag cctggcgtcc tcgcaaacat ga
Details for LetaA.19501.a.B1.GE40874
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 75kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SKQTEGTTAD EERQRTSVSS SDSSSVSTTD SDEVSSGASD SSSSGSGSTS ASESSDRERP SSLSQRQNTA TRERPTSTQQ LQQQRHVQLN PELRFERPDK EYIPLPPLRD MQDMSKVLFL LSTDKKRYPD GRHRTLDYFR ASVEMFVTEV RQEYKRQYQQ AQRGGRAMQR FTWKNSGELA ICFACCCDNV KLLYDSLQPG PLKPLWDAFV SQLAPMLIIQ SRVPEMMLSS QTYHTKYMDW VKGGTRYGGE QSTANVRFPS VADRRVKVET YLRSGAGYKI EAVQVGLTAA VPAAAPVAAG SHRSSTAASA PVQATETRAP FAGRLPGTGF AKVPVSPGIG GPRPPLKVPM KHAGGGAAQG SVTGAAAPPL ASTCVKQMPP EMKHLFWLSV LAHVKGGDAS RAFPDDRLEV LPGLLRLCEM TEHTEEMCIL MDVLAASSSG VQTVFERLGG LLSFRRFASH FIQREEAEPL MQIIEQLARW RVVAWGSTSQ QAWQTDMNQR RTRDLMWLRT LPLIPEEKRA AWGALLLVME QRYIFKAPRP DDTTTMDNPL KRRRPEIELG DLHAVLSDNV DMQKWVVPRV NVFSAVRLPV ELRVPPLCTK SEEDTVRFLN SKREYAEKAQ VAWRERLEKI LCSQPHALDS LEPVPLWYDP YALLSLASSQ T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct caaaacaaac tgagggcaca actgctgacg aggagcgcca gcgaacttcg gtctcgtcta gcgactcgtc gagtgtcagc actacagact ctgacgaagt cagcagcggt gcctctgaca gcagcagcag cggcagcggc agcacctcgg cctccgaatc atctgataga gagcggccct cgtctctctc acagcgtcaa aacaccgcta caagagagag gccgacgtcc acacagcagc tacagcagca gcggcatgtg cagctgaatc ccgagctgcg cttcgagcgg ccagacaagg agtacatccc actgccaccc ctaagagaca tgcaggacat gtccaaggtt ctgtttctct tgagcaccga taagaaacgc tacccggatg gtcgccaccg cacgctggac tacttccgtg cctcggtgga gatgtttgtg acggaggttc ggcaggagta caaacggcag taccagcagg cgcagcgcgg tggccgtgca atgcagcggt tcacctggaa gaactcgggc gagctagcta tttgctttgc ctgctgctgc gacaacgtga agcttctgta tgacagcctc caacctggcc cactcaagcc actctgggac gcctttgtca gccagctcgc gccgatgctc atcattcaga gtcgcgtgcc ggaaatgatg ctgtcatctc aaacttacca caccaagtat atggactggg taaagggtgg cacacgctac ggaggagagc agtcgactgc gaatgtccgc ttcccctccg tggcagaccg tcgcgtgaag gttgaaacat acctacggag cggtgcaggg tacaagatcg aggctgtgca ggtggggttg acggccgccg tgcccgcggc tgctccagtc gcggcggggt ctcaccgttc ttcgactgct gcttcggctc cggtgcaggc tactgagact cgggcgcctt tcgcggggcg gctgcccggc acaggttttg caaaggtgcc ggtctctccc ggcatcggtg gacctcgccc gccactgaag gtgcctatga agcacgctgg cggcggtgct gcacagggca gtgtgacggg ggccgccgcg cctccgcttg cctccacctg tgtgaagcag atgcccccgg agatgaagca tttgttttgg ctgtcggttc ttgcgcacgt aaagggcggg gatgcgtccc gggcgtttcc ggacgaccga ctggaggtgc tcccgggcct gctgcggctg tgcgagatga cggagcacac ggaggagatg tgcattttga tggacgtact ggcggcgtcc agcagtggcg tccagaccgt ctttgaaaga cttggcggtc tgctgtcatt ccgccggttc gcctcccact ttatccagcg tgaggaggca gagccactca tgcaaatcat cgagcagctc gcgcggtggc gcgttgtggc atggggcagt acctcacagc aggcatggca gaccgatatg aaccaacggc gcacacgtga tctgatgtgg cttcgtactc tgcccctcat cccggaggag aagcgggcag cgtggggtgc gctactgctg gtgatggagc aacgttacat tttcaaggct ccgcgccctg atgacacgac caccatggat aatccactga agcgtcggcg acccgagatc gagctcggcg acctgcatgc tgtgttgagt gacaacgtcg acatgcaaaa atgggtggtt ccccgagtca atgtgttctc cgctgtccgc ctaccggtgg agctgcgcgt gccaccactg tgtacaaagt ctgaggagga taccgtgcgc tttctcaact ctaagcgcga gtatgccgaa aaggcgcagg tggcgtggcg tgagagacta gagaagattt tgtgctcgca gcctcatgcg ttggattccc ttgaaccggt gcctctctgg tatgacccgt acgcgctgct gagcctggcg tcctcgcaaa catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LetaA.19501.a.B2.GE40707
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK HLFWLSVLAH VKGGDASRAF PDDRLEVLPG LLRLCEMTEH TEEMCILMDV LAASSSGVQT VFERLGGLLS FRRFASHFIQ REEAEPLMQI IEQLARWRVV AWGSTSQQAW QTDMNQRRTR DLMWLRTLPL IPEEKRAAWG ALLLVMEQRY IFKAPRPDDT TTMDNPLKRR RPEIELGDLH AVLSDNVDMQ KWVVPRVNVF SAVRLPVELR VPPLCTKSEE DTVRFLNSKR EYAEKAQVAW RERLEKILCS QPHALDSLEP VPLWYXXYAL LS
Validated NT Sequence
atggctcacc accaccacca ccatatgaag catttgtttt ggctgtcggt tcttgcgcac gtaaagggcg gggatgcgtc ccgggcgttt ccggacgacc gactggaggt gctcccgggc ctgctgcggc tgtgcgagat gacggagcac acggaggaga tgtgcatttt gatggacgta ctggcggcgt ccagcagtgg cgtccagacc gtctttgaaa gacttggcgg tctgctgtca ttccgccggt tcgcctccca ctttatccag cgtgaggagg cagagccact catgcaaatc atcgagcagc tcgcgcggtg gcgcgttgtg gcatggggca gtacctcaca gcaggcatgg cagaccgata tgaaccaacg gcgcacacgt gatctgatgt ggcttcgtac tctgcccctc atcccggagg agaagcgggc agcgtggggt gcgctactgc tggtgatgga gcaacgttac attttcaagg ctccgcgccc tgatgacacg accaccatgg ataatccact gaagcgtcgg cgacccgaga tcgagctcgg cgacctgcat gctgtgttga gtgacaacgt cgacatgcaa aaatgggtgg ttccccgagt caatgtgttc tccgctgtcc gcctaccggt ggagctgcgc gtgccaccac tgtgtacaaa gtctgaggag gataccgtgc gctttctcaa ctctaagcgc gagtatgccg aaaaggcgca ggtggcgtgg cgtgagagac tagagaagat tttgtgctcg cagcctcatg cgttggattc ccttgaaccg gtgcctctct ggtatgnnnn ntacgcgctg ctgagctaag tgagtaa
Expected Protein Sequence
MAHHHHHHMK HLFWLSVLAH VKGGDASRAF PDDRLEVLPG LLRLCEMTEH TEEMCILMDV LAASSSGVQT VFERLGGLLS FRRFASHFIQ REEAEPLMQI IEQLARWRVV AWGSTSQQAW QTDMNQRRTR DLMWLRTLPL IPEEKRAAWG ALLLVMEQRY IFKAPRPDDT TTMDNPLKRR RPEIELGDLH AVLSDNVDMQ KWVVPRVNVF SAVRLPVELR VPPLCTKSEE DTVRFLNSKR EYAEKAQVAW RERLEKILCS QPHALDSLEP VPLWYDPYAL LS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc atttgttttg gctgtcggtt cttgcgcacg taaagggcgg ggatgcgtcc cgggcgtttc cggacgaccg actggaggtg ctcccgggcc tgctgcggct gtgcgagatg acggagcaca cggaggagat gtgcattttg atggacgtac tggcggcgtc cagcagtggc gtccagaccg tctttgaaag acttggcggt ctgctgtcat tccgccggtt cgcctcccac tttatccagc gtgaggaggc agagccactc atgcaaatca tcgagcagct cgcgcggtgg cgcgttgtgg catggggcag tacctcacag caggcatggc agaccgatat gaaccaacgg cgcacacgtg atctgatgtg gcttcgtact ctgcccctca tcccggagga gaagcgggca gcgtggggtg cgctactgct ggtgatggag caacgttaca ttttcaaggc tccgcgccct gatgacacga ccaccatgga taatccactg aagcgtcggc gacccgagat cgagctcggc gacctgcatg ctgtgttgag tgacaacgtc gacatgcaaa aatgggtggt tccccgagtc aatgtgttct ccgctgtccg cctaccggtg gagctgcgcg tgccaccact gtgtacaaag tctgaggagg ataccgtgcg ctttctcaac tctaagcgcg agtatgccga aaaggcgcag gtggcgtggc gtgagagact agagaagatt ttgtgctcgc agcctcatgc gttggattcc cttgaaccgg tgcctctctg gtatgacccg tacgcgctgc tgagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LetaA.19501.a.B3.GE40708
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMSKVLFLL STDKKRYPDG RHRTLDYFRA SVEMFVTEVR QEYKRQYQQA QRGGRAMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVS QLAPMLIIQS RVPEMMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atggctcacc accaccacca ccatatgcta cagcagcagc ggcatgtgca gctgaatccc gagctgcgct tcgagcggcc agacaaggag tacatcccac tgccacccct aagagacatg caggacatgt ccaaggttct gtttctcttg agcaccgata agaaacgcta cccggatggt cgccaccgca cgctggacta cttccgtgcc tcggtggaga tgtttgtgac ggaggttcgg caggagtaca aacggcagta ccagcaggcg cagcgcggtg gccgtgcaat gcagcggttc acctggaaga actcgggcga gctagctatt tgctttgcct gctgctgcga caacgtgaag cttctgtatg acagcctcca acctggccca ctcaagccac tctgggacgc ctttgtcagc cagctcgcgc cgatgctcat cattcagagt cgcgtgccgg aaatgatgct gtcatctcaa acttaccaca ccaagtatat ggactgggta aagggtggca cacgctacgg aggagagcag tcgactgcga atgtccgctt cccctccgtg gcagaccgtc gcgtgaaggt tgaaacatac ctacggagcg gtgcagggta caagatcgag gcttaagtga gtaagat
Expected Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMSKVLFLLS TDKKRYPDGR HRTLDYFRAS VEMFVTEVRQ EYKRQYQQAQ RGGRAMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVSQ LAPMLIIQSR VPEMMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctacagc agcagcggca tgtgcagctg aatcccgagc tgcgcttcga gcggccagac aaggagtaca tcccactgcc acccctaaga gacatgcagg acatgtccaa ggttctgttt ctcttgagca ccgataagaa acgctacccg gatggtcgcc accgcacgct ggactacttc cgtgcctcgg tggagatgtt tgtgacggag gttcggcagg agtacaaacg gcagtaccag caggcgcagc gcggtggccg tgcaatgcag cggttcacct ggaagaactc gggcgagcta gctatttgct ttgcctgctg ctgcgacaac gtgaagcttc tgtatgacag cctccaacct ggcccactca agccactctg ggacgccttt gtcagccagc tcgcgccgat gctcatcatt cagagtcgcg tgccggaaat gatgctgtca tctcaaactt accacaccaa gtatatggac tgggtaaagg gtggcacacg ctacggagga gagcagtcga ctgcgaatgt ccgcttcccc tccgtggcag accgtcgcgt gaaggttgaa acatacctac ggagcggtgc agggtacaag atcgaggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LetaA.19501.a.B4.GE40709
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KVLFLLSTDK KRYPDGRHRT LDYFRASVEM FVTEVRQEYK RQYQQAQRGG RAMQRFTWKN SGELAICFAC CCDNVKLLYD SLQPGPLKPL WDAFVSQLAP MLIIQSRVPE MMLSSQTYHT KYMDWVKGGT RYGGEQSTAN VRFPSVADRR VKVETYLRSG A
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc aaggttctgt ttctcttgag caccgataag aaacgctacc cggatggtcg ccaccgcacg ctggactact tccgtgcctc ggtggagatg tttgtgacgg aggttcggca ggagtacaaa cggcagtacc agcaggcgca gcgcggtggc cgtgcaatgc agcggttcac ctggaagaac tcgggcgagc tagctatttg ctttgcctgc tgctgcgaca acgtgaagct tctgtatgac agcctccaac ctggcccact caagccactc tgggacgcct ttgtcagcca gctcgcgccg atgctcatca ttcagagtcg cgtgccggaa atgatgctgt catctcaaac ttaccacacc aagtatatgg actgggtaaa gggtggcaca cgctacggag gagagcagtc gactgcgaat gtccgcttcc cctccgtggc agaccgtcgc gtgaaggttg aaacatacct acggagcggt gcataagtga gtaagat
Expected Protein Sequence
MAHHHHHHSK VLFLLSTDKK RYPDGRHRTL DYFRASVEMF VTEVRQEYKR QYQQAQRGGR AMQRFTWKNS GELAICFACC CDNVKLLYDS LQPGPLKPLW DAFVSQLAPM LIIQSRVPEM MLSSQTYHTK YMDWVKGGTR YGGEQSTANV RFPSVADRRV KVETYLRSGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaagg ttctgtttct cttgagcacc gataagaaac gctacccgga tggtcgccac cgcacgctgg actacttccg tgcctcggtg gagatgtttg tgacggaggt tcggcaggag tacaaacggc agtaccagca ggcgcagcgc ggtggccgtg caatgcagcg gttcacctgg aagaactcgg gcgagctagc tatttgcttt gcctgctgct gcgacaacgt gaagcttctg tatgacagcc tccaacctgg cccactcaag ccactctggg acgcctttgt cagccagctc gcgccgatgc tcatcattca gagtcgcgtg ccggaaatga tgctgtcatc tcaaacttac cacaccaagt atatggactg ggtaaagggt ggcacacgct acggaggaga gcagtcgact gcgaatgtcc gcttcccctc cgtggcagac cgtcgcgtga aggttgaaac atacctacgg agcggtgcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LetaA.19501.a.B3.PS38165
PURIFICATION DATe: 1/11/2017
CONCENTRATION: 51mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 20
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMSKVLFLL STDKKRYPDG RHRTLDYFRA SVEMFVTEVR QEYKRQYQQA QRGGRAMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVS QLAPMLIIQS RVPEMMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atggctcacc accaccacca ccatatgcta cagcagcagc ggcatgtgca gctgaatccc gagctgcgct tcgagcggcc agacaaggag tacatcccac tgccacccct aagagacatg caggacatgt ccaaggttct gtttctcttg agcaccgata agaaacgcta cccggatggt cgccaccgca cgctggacta cttccgtgcc tcggtggaga tgtttgtgac ggaggttcgg caggagtaca aacggcagta ccagcaggcg cagcgcggtg gccgtgcaat gcagcggttc acctggaaga actcgggcga gctagctatt tgctttgcct gctgctgcga caacgtgaag cttctgtatg acagcctcca acctggccca ctcaagccac tctgggacgc ctttgtcagc cagctcgcgc cgatgctcat cattcagagt cgcgtgccgg aaatgatgct gtcatctcaa acttaccaca ccaagtatat ggactgggta aagggtggca cacgctacgg aggagagcag tcgactgcga atgtccgctt cccctccgtg gcagaccgtc gcgtgaaggt tgaaacatac ctacggagcg gtgcagggta caagatcgag gcttaagtga gtaagat
Expressed Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMSKVLFLLS TDKKRYPDGR HRTLDYFRAS VEMFVTEVRQ EYKRQYQQAQ RGGRAMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVSQ LAPMLIIQSR VPEMMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTACAGC AGCAGCGGCA TGTGCAGCTG AATCCCGAGC TGCGCTTCGA GCGGCCAGAC AAGGAGTACA TCCCACTGCC ACCCCTAAGA GACATGCAGG ACATGTCCAA GGTTCTGTTT CTCTTGAGCA CCGATAAGAA ACGCTACCCG GATGGTCGCC ACCGCACGCT GGACTACTTC CGTGCCTCGG TGGAGATGTT TGTGACGGAG GTTCGGCAGG AGTACAAACG GCAGTACCAG CAGGCGCAGC GCGGTGGCCG TGCAATGCAG CGGTTCACCT GGAAGAACTC GGGCGAGCTA GCTATTTGCT TTGCCTGCTG CTGCGACAAC GTGAAGCTTC TGTATGACAG CCTCCAACCT GGCCCACTCA AGCCACTCTG GGACGCCTTT GTCAGCCAGC TCGCGCCGAT GCTCATCATT CAGAGTCGCG TGCCGGAAAT GATGCTGTCA TCTCAAACTT ACCACACCAA GTATATGGAC TGGGTAAAGG GTGGCACACG CTACGGAGGA GAGCAGTCGA CTGCGAATGT CCGCTTCCCC TCCGTGGCAG ACCGTCGCGT GAAGGTTGAA ACATACCTAC GGAGCGGTGC AGGGTACAAG ATCGAGGCTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT