SSGCID: Target Page

LetaA.19437.a

Serine/threonine-protein kinase, putative (AUK2)

CENTER ID:	LetaA.19437.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19437.a.B2.GE42826	PHoranyi kinase domain truncation		237	566

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.19437.a.B2.PW38678	PHoranyi kinase domain truncation		237	566

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP29.1490

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSPCPSSSS PFLGGVTSGI RSPSRDPLPL LLHHRHAPMR LRCREDGTMR ACPEKFGKDV DQACPSPLPT ASVFSREKGE PDAKRHHAEP SSCLRPGGGG SAASQNAAAT TAKIAAPLLS TSNPSSREAL SPFRFLCTSS RGCPVAEADG ARVHASDLLA RIDRRGSLAP RDFSTTPADL NVHSTAAVVR ASPSSGAASP LLSATCPSMV SGASTETSTP SRVLSRPHRI HQHELRPADF HREEVLGEGS YSIVTLATHR ASGLSFALKE IDRSRLQWRP LEAQLRWEIN LQRTLRHPHI VRLYSYFITP DCISLVLEYC SGGTLLSRLR AAPHHRFSER QASRYTRHVA KALTHLHGLG VAHRDLKLEN VLISADDIAK LADFGWSRPV VHPLPPNGSA ASAQTAGESN SVPRDSAVHH TSNDGPAEEL AEGRRTVCGT LDYLSPEMVS GQVHSAKTDV WSLGVMLAEM LTGTPPFYSE STHETLYAIK RLPPNLSGVH STRRGPVLTG SGASATANVC PTHEELVALS PDALSLICAM LQKDPSARPT MSEVLGHPWL QKPVRRS

NT Sequence

atgtcgtcgc catgcccttc ctcatcctcg ccttttctag gaggcgtaac cagcggtata cgatcaccgt caagagatcc actaccgctg ctgctgcacc atcgacatgc cccgatgcga ctgcgttgcc gtgaggacgg taccatgcgc gcatgtccag agaagtttgg caaggatgta gaccaggcat gcccttcccc gctgccgact gcttcagttt tttctcgaga gaaaggtgaa ccggacgcaa agcgtcatca tgcagagccg tcatcttgcc tcagaccagg cggcggcgga agcgctgcca gccagaatgc ggcggcgaca acagctaaga ttgctgctcc actgctttct acatccaacc cctcctcccg ggaagcactt tcgccttttc gtttcctgtg cacatcttcg agggggtgtc cggtggccga ggcggacggt gcacgagttc acgcgtcaga cttgctagcg aggattgaca ggcgcggcag cctcgctccg agggactttt cgaccacacc agcagacttg aacgtccatt ccacggcggc agttgtgcgg gcctcgccct cctccggtgc cgcatcacct ctgctctctg cgacttgccc ctccatggta tctggcgcca gcactgaaac atcaacaccg tcgcgagtgc tgtcgcggcc tcaccgcata catcaacacg agctgcgccc cgcggatttc catcgtgagg aggttctcgg tgagggtagc tactcgattg ttacccttgc cacgcaccgt gcgtcgggcc tgtcctttgc gctgaaggag attgaccgca gccggctgca atggcgaccg ctggaggcgc agctgcgctg ggagatcaat ctgcagcgga cgctgcgcca cccgcacatt gtgcgtctct acagctactt catcacgcca gactgcatca gtcttgtact ggagtactgc agcggtggga cgctgctgag tcgcttgaga gctgcaccac accaccggtt ttcggagcgg caggcatcac gctacactcg ccatgtggcg aaggcgctga cgcatttgca cgggctcgga gtggcgcacc gagacttgaa gctggaaaat gtgctcatca gcgcagacga cattgccaag ctggcagact ttggctggtc gcgccctgtc gtgcatcctc tgccacccaa tgggtcagcg gccagtgctc agactgcagg cgagagcaac agcgtaccgc gcgacagcgc ggtgcaccac acgagcaacg atggaccagc agaagagctc gcagagggtc gccgcactgt gtgtggcacg ctggattacc tctcgcctga gatggtctcc ggccaagtac actcagccaa gacagacgtg tggtcgcttg gtgtcatgct ggccgagatg ctgaccggca cgccaccgtt ctacagcgag tcgactcacg agacgctgta cgccataaag cggttgccac caaacctcag cggcgtccac tccacacgcc gcgggccagt gctcactggt agcggtgcct ctgccacagc taatgtgtgt ccgacccatg aagaacttgt tgccctatct ccagatgcgc tctctctcat ttgcgctatg ctccagaagg acccatcggc gcgaccgacg atgtcggagg tgctgggcca cccgtggcta caaaaacccg ttaggaggtc atga

Details for LetaA.19437.a.B2.GE42826

HARVESTED ON:	1/9/2019
SEQUENCED ON:	1/15/2019
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	75
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMP AYFHREEVLG EGSYSIVTLA THRASGLSFA LKEIDRSRLQ WRPLEAQLRW EINLQRTLRH PHIVRLYSYF ITPDCISLVL EYCSGGTLLS LLRAAPHHRF SERQASRYTR HVAKALTHLH GLGVAHRDLK LENVLISADD IAKLADFGWS RPVVHPLPPN GSAASAQTAG ESNSVPRDSA VHNTSNDGPA EKLAEGRRTV CGTLDYLSRE MVSGQVHFSQ DRRVVAWCHA GRDADRHATV LQRVDSRDAV RHKAVATKPQ RRPLHSPRAS AHW

Validated NT Sequence

gtggcccagn anctccgaca tcgtcgnnng cnnnnnnnnn nccttctngn nnnnnnnnna antnnnnnag agnnnnnctg gngnnnnngn nnnnnnttca tcannnnnnn nnnnnnnnnn nnnnntggcn nnnncaccgc taccnnngag cactggcccg cggcnnnngg agtggacgcn gcnnnnntnn ngtggnnnnc gctttatggc gtacagcgtc tcnnnngtcg actcgctgta gaacgnnnnn gtgccggtca gcatctcggc cagcntgana ccaagcgacc annngtctgt cttggctgnn gtgtacttgg ccggagacca tctnnnnnna gaggtaatcc annntgccac acacagtgcg gcgaccctct gcgagctnnn ntgctggtcc atcgttgctc gtgttgtgca ccgcgctgtc gcgcggtacg ctgttgctct cnnnngcagt ctgagcactg gccgctgacc cattgggtgg cagaggatgc acgacagggc gcgaccagnn aaagtctgcc agcttggcaa tgtcgtctgc gctgatgagc acattttcca gcttcaagtc tcggtgcgcc acaccgagcc cgtgcaaatg cgtcagcgcc ttcgccacat ggcgagtgta nnntgatgcc tgccgctccg aaaaccggtg gtgtggtgca gctctcaaga gactcagcag cgtcccaccg ctgcagtact ccagtacaag actgatgcag tctggcgtga tgaagtagct gtagagacgc acaatgtgcg ggtggcgcag cgtccgctgc agatnnatct cccagcgcan nngcgcctcc agcggtcgcc attgcagccg gnngcggtca atctnnntca gcgcaaagga caggcccgac gcacggtgcg tggcaagggt aacaatcgag tannnaccct caccgagaac ctcctcacga tggaannnnn cgggcatatg gtggtggtgg tggtgagcca tggta

Expected Protein Sequence

MAHHHHHHPA DFHREEVLGE GSYSIVTLAT HRASGLSFAL KEIDRSRLQW RPLEAQLRWE INLQRTLRHP HIVRLYSYFI TPDCISLVLE YCSGGTLLSR LRAAPHHRFS ERQASRYTRH VAKALTHLHG LGVAHRDLKL ENVLISADDI AKLADFGWSR PVVHPLPPNG SAASAQTAGE SNSVPRDSAV HHTSNDGPAE ELAEGRRTVC GTLDYLSPEM VSGQVHSAKT DVWSLGVMLA EMLTGTPPFY SESTHETLYA IKRLPPNLSG VHSTRRGPVL TGSGASATAN VCPTHEELVA LSPDALSLIC AMLQKDPSAR PTMSEVLGHP WLQKPVRR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcccgcgg atttccatcg tgaggaggtt ctcggtgagg gtagctactc gattgttacc cttgccacgc accgtgcgtc gggcctgtcc tttgcgctga aggagattga ccgcagccgg ctgcaatggc gaccgctgga ggcgcagctg cgctgggaga tcaatctgca gcggacgctg cgccacccgc acattgtgcg tctctacagc tacttcatca cgccagactg catcagtctt gtactggagt actgcagcgg tgggacgctg ctgagtcgct tgagagctgc accacaccac cggttttcgg agcggcaggc atcacgctac actcgccatg tggcgaaggc gctgacgcat ttgcacgggc tcggagtggc gcaccgagac ttgaagctgg aaaatgtgct catcagcgca gacgacattg ccaagctggc agactttggc tggtcgcgcc ctgtcgtgca tcctctgcca cccaatgggt cagcggccag tgctcagact gcaggcgaga gcaacagcgt accgcgcgac agcgcggtgc accacacgag caacgatgga ccagcagaag agctcgcaga gggtcgccgc actgtgtgtg gcacgctgga ttacctctcg cctgagatgg tctccggcca agtacactca gccaagacag acgtgtggtc gcttggtgtc atgctggccg agatgctgac cggcacgcca ccgttctaca gcgagtcgac tcacgagacg ctgtacgcca taaagcggtt gccaccaaac ctcagcggcg tccactccac acgccgcggg ccagtgctca ctggtagcgg tgcctctgcc acagctaatg tgtgtccgac ccatgaagaa cttgttgccc tatctccaga tgcgctctct ctcatttgcg ctatgctcca gaaggaccca tcggcgcgac cgacgatgtc ggaggtgctg ggccacccgt ggctacaaaa acccgttagg aggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for LetaA.19437.a.B2.PW38678

PURIFICATION DATe:	8/3/2019
CONCENTRATION:	17mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	130µl
PERCENT IDENTITY:	75
PERCENT COVERAGE:	83

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCCCGCGG ATTTCCATCG TGAGGAGGTT CTCGGTGAGG GTAGCTACTC GATTGTTACC CTTGCCACGC ACCGTGCGTC GGGCCTGTCC TTTGCGCTGA AGGAGATTGA CCGCAGCCGG CTGCAATGGC GACCGCTGGA GGCGCAGCTG CGCTGGGAGA TCAATCTGCA GCGGACGCTG CGCCACCCGC ACATTGTGCG TCTCTACAGC TACTTCATCA CGCCAGACTG CATCAGTCTT GTACTGGAGT ACTGCAGCGG TGGGACGCTG CTGAGTCGCT TGAGAGCTGC ACCACACCAC CGGTTTTCGG AGCGGCAGGC ATCACGCTAC ACTCGCCATG TGGCGAAGGC GCTGACGCAT TTGCACGGGC TCGGAGTGGC GCACCGAGAC TTGAAGCTGG AAAATGTGCT CATCAGCGCA GACGACATTG CCAAGCTGGC AGACTTTGGC TGGTCGCGCC CTGTCGTGCA TCCTCTGCCA CCCAATGGGT CAGCGGCCAG TGCTCAGACT GCAGGCGAGA GCAACAGCGT ACCGCGCGAC AGCGCGGTGC ACCACACGAG CAACGATGGA CCAGCAGAAG AGCTCGCAGA GGGTCGCCGC ACTGTGTGTG GCACGCTGGA TTACCTCTCG CCTGAGATGG TCTCCGGCCA AGTACACTCA GCCAAGACAG ACGTGTGGTC GCTTGGTGTC ATGCTGGCCG AGATGCTGAC CGGCACGCCA CCGTTCTACA GCGAGTCGAC TCACGAGACG CTGTACGCCA TAAAGCGGTT GCCACCAAAC CTCAGCGGCG TCCACTCCAC ACGCCGCGGG CCAGTGCTCA CTGGTAGCGG TGCCTCTGCC ACAGCTAATG TGTGTCCGAC CCATGAAGAA CTTGTTGCCC TATCTCCAGA TGCGCTCTCT CTCATTTGCG CTATGCTCCA GAAGGACCCA TCGGCGCGAC CGACGATGTC GGAGGTGCTG GGCCACCCGT GGCTACAAAA ACCCGTTAGG AGGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT