LetaA.18708.a

Isoleucyl-tRNA synthetase, putative

CENTER ID: LetaA.18708.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.18708.a.B3.GE42822 soluble domain #2 939 1225
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP36.5770

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRCTTQLSR GYGFGVFACT AARLKKKNEK SSKPYPTATR TVIYSHGKPV NYSAASTVAT APSASFPPTP PSASHPSSTA LGSTLRTDVS QATPNANSQN LDSSSRPSPR PSSPNFPLLP SHPRLSFHMT GEGKQQQDPP AGQQGVTVKN YPDVFNFPEM EEQVLAMWHE KDCFRTSMKL SEGREPFSFY DGPPFATGLP HYGHILAGTI KDMVTRFAYQ TNHHVIRRFG WDCHGLPVEY EIDKALGIKT SHDVAKLGID KYNDECKKIV TRYVDEWRTT VERVGRWIDF DNDYKTMHLT YMESVWWVFS QLWEKKLVYR GFRVMPYSTA CTTPLSNFEA NSNYKEVRDI AVTVAFQSRA DPNTYFLAWT TTPWTLPSNL SLCVHPELDY VKVLDTKSKR HYWLAEDRLA EVYPKKDKKA SKAKDADNGE EANATPYTVV EKLKGAQLVG MKYVPIFPYF ESSMSSTAFR VIPGTYVTTD AGTGIVHQAP AFGEDDYQAC LDAGIVEKGG KFVCPVDENG MFTSEVADFA GKYVKDADPE IIKVLETRGQ LFHKASIVHS YPFCWRSDTP LIYKAVESWF VNVESFRDRL IECNEKAYWV PDFVKTRRFS NWLAEARDWN VSRNRYWGTP MPVWHSEDWE EVVCISSIKQ LEELSGVTGI EDIHRQFVDN ITIPSKRPGM PPLKRVPMVF DCWFESGSMP YAQEHFPFEN QDKFKDLFPA DFVSEGLDQT RGWFYTLLVL GVALFDVCPF RNVAVSGLVL AEDGKKMSKR LKNYPEPKVV IDTYGADALR MYMISSPVVR AEPLRFREAG VKGVVKDILL PLFNAAKFFI SNANYCIESG GQVSLQVRST NEMDRWILAS CQSLLRYVKQ EMKLYHLYNV VPGILRFVGD LSNWYVRMNR RRMKNATDSE DRAQALSTML YLLFSVSRIV GHIAPFVAEM LYQQIKPLLP ANEQVDSVHY LMIPDEDASL DDPELERAMS RMMNIVDLVR VLRDQMVIPI KRPVRQVVIV HPDQQYIDDV RKVADYIKDE VNAFEIVMSS GEDYVETKLD ANFEVLGKKY RKEMPAIRKG IQAMTQEEVA KFLHDKKGVV VGKELTIEDV KVLRQVKDGI TDYQSHSDSD VVVLVDKRQD QELIDSWRAR EFVNRVQQLR KKAKLVVTDE VDVYFEAEEA DLTASILNSK NQINQTLRGV WATMDQKPSD AELVAEEDNS ISGVTVRLVF TKPKA
NT Sequence
ATGCGCCGGT GCACCACACA GCTCTCGCGA GGCTACGGTT TCGGTGTCTT TGCGTGTACG GCAGCGAGGC TAAAGAAGAA GAACGAGAAA AGTAGTAAAC CATACCCCAC TGCAACCCGC ACAGTTATCT ACTCGCACGG AAAGCCTGTG AATTACTCAG CAGCCTCTAC TGTGGCGACT GCTCCCTCCG CCTCTTTCCC TCCTACCCCT CCCTCTGCTT CACATCCGTC ATCGACCGCC CTGGGCAGCA CGCTCCGCAC CGACGTCAGT CAAGCTACAC CTAACGCCAA CAGCCAAAAT CTGGACTCCA GCTCCCGCCC CTCTCCCCGT CCGTCGTCCC CGAACTTCCC CTTGCTTCCC TCACACCCCA GATTGTCGTT TCACATGACG GGCGAGGGTA AGCAGCAGCA AGACCCCCCA GCGGGGCAGC AGGGCGTAAC GGTGAAGAAC TATCCCGATG TGTTCAACTT CCCGGAAATG GAGGAACAGG TGCTGGCGAT GTGGCACGAG AAGGATTGCT TTCGCACTTC AATGAAGCTC TCCGAGGGTC GAGAGCCATT CAGCTTCTAC GATGGCCCCC CGTTCGCGAC TGGTCTGCCG CACTACGGCC ACATCTTGGC CGGTACCATC AAGGATATGG TCACTCGCTT CGCCTACCAG ACGAACCATC ACGTTATCCG ACGCTTTGGC TGGGATTGTC ACGGGCTTCC GGTGGAGTAC GAGATCGACA AGGCGCTAGG CATCAAGACG TCGCACGACG TCGCGAAGCT CGGCATCGAC AAGTATAACG ACGAGTGCAA GAAGATCGTC ACCCGTTACG TTGACGAGTG GAGGACGACT GTCGAGCGCG TCGGTCGCTG GATCGACTTC GACAACGACT ACAAGACGAT GCATCTCACA TACATGGAGA GTGTCTGGTG GGTCTTCTCG CAGTTGTGGG AGAAGAAGCT GGTCTACCGC GGCTTCCGCG TGATGCCGTA CTCAACCGCC TGTACGACGC CACTGAGCAA CTTTGAGGCG AACTCTAACT ACAAGGAGGT CAGAGACATC GCTGTGACAG TCGCCTTCCA GTCTCGCGCC GATCCAAACA CATACTTTCT TGCCTGGACC ACCACGCCAT GGACGTTACC GAGCAACCTC AGTCTGTGCG TGCACCCCGA GCTAGACTAC GTGAAGGTGT TGGACACCAA GTCGAAGCGT CACTACTGGC TTGCGGAGGA TCGCCTGGCG GAGGTGTATC CAAAGAAGGA CAAGAAGGCC TCAAAGGCGA AGGACGCCGA CAATGGCGAG GAAGCCAATG CTACGCCGTA CACGGTCGTC GAGAAGCTCA AGGGCGCGCA ACTAGTGGGC ATGAAATACG TGCCGATTTT CCCTTACTTT GAGTCGTCCA TGAGTTCCAC GGCCTTCCGC GTCATCCCTG GTACCTACGT GACAACAGAC GCTGGTACGG GTATTGTGCA TCAGGCCCCT GCCTTCGGTG AAGATGATTA CCAGGCGTGC CTCGACGCTG GGATCGTTGA GAAAGGGGGA AAGTTTGTCT GCCCCGTTGA CGAAAACGGC ATGTTCACAA GCGAGGTGGC GGATTTCGCT GGCAAGTACG TCAAGGATGC GGATCCAGAG ATTATCAAAG TGCTGGAGAC GCGGGGGCAG CTCTTTCACA AGGCGTCTAT TGTGCACAGC TACCCGTTTT GCTGGCGCAG CGACACCCCA CTCATCTACA AGGCTGTCGA GTCGTGGTTC GTCAACGTTG AGTCTTTCCG CGACCGCCTC ATCGAATGCA ACGAAAAGGC GTACTGGGTA CCTGATTTTG TCAAGACCCG CCGCTTCTCG AACTGGCTCG CGGAGGCGCG CGACTGGAAC GTGTCACGCA ACCGCTACTG GGGCACGCCG ATGCCGGTGT GGCACAGCGA GGACTGGGAG GAGGTTGTGT GCATCAGCAG CATCAAACAG CTCGAGGAAC TCTCCGGTGT CACCGGCATC GAGGACATTC ACCGCCAGTT TGTCGACAAC ATCACGATCC CTAGTAAACG GCCCGGCATG CCACCGCTGA AGCGTGTGCC GATGGTGTTC GACTGCTGGT TTGAGTCCGG CTCGATGCCG TATGCCCAGG AACACTTTCC GTTCGAGAAT CAGGACAAGT TTAAGGACCT GTTTCCGGCA GACTTCGTGT CAGAGGGTTT GGACCAGACG CGCGGTTGGT TCTACACCCT TCTCGTTCTC GGTGTGGCAC TCTTTGACGT CTGCCCTTTC CGAAATGTCG CCGTCAGCGG GCTCGTTCTA GCTGAGGACG GGAAGAAGAT GAGCAAGCGC CTCAAGAACT ACCCCGAACC AAAGGTGGTG ATTGACACCT ACGGCGCGGA TGCCCTGCGC ATGTACATGA TCAGCTCGCC GGTCGTGCGC GCCGAGCCAC TGCGTTTCCG AGAGGCAGGT GTAAAAGGCG TTGTCAAGGA CATACTACTG CCTCTGTTCA ACGCCGCGAA GTTCTTCATC TCTAATGCGA ACTACTGCAT TGAGTCCGGT GGTCAGGTAT CGCTCCAAGT TCGCAGCACA AACGAAATGG ACCGCTGGAT TCTCGCCTCG TGCCAGAGTC TGCTGCGCTA CGTGAAGCAG GAGATGAAGC TGTACCACCT GTACAACGTC GTTCCGGGCA TTCTGCGTTT TGTGGGTGAC CTGTCCAACT GGTACGTGCG CATGAACCGC CGTCGCATGA AGAATGCCAC GGACAGCGAG GACCGCGCAC AGGCGCTGTC GACGATGTTG TACCTCCTTT TCTCAGTGTC CCGCATTGTG GGCCACATCG CCCCGTTTGT GGCAGAAATG CTTTACCAGC AAATCAAGCC CCTCCTCCCG GCGAATGAGC AAGTGGACTC CGTGCACTAC CTCATGATCC CCGACGAGGA CGCCTCCTTG GACGACCCGG AGTTGGAGCG TGCCATGTCA CGCATGATGA ACATTGTGGA TTTGGTACGT GTGTTGCGTG ATCAGATGGT CATTCCGATT AAGCGGCCGG TGCGTCAGGT TGTCATTGTT CACCCAGACC AGCAATACAT TGACGACGTG CGCAAGGTAG CGGACTACAT TAAGGATGAG GTGAACGCAT TCGAAATCGT CATGTCGAGT GGCGAAGATT ACGTGGAGAC GAAACTGGAC GCAAACTTTG AGGTACTTGG CAAGAAGTAC CGCAAGGAGA TGCCTGCGAT CCGCAAGGGT ATCCAGGCTA TGACGCAGGA GGAGGTGGCC AAGTTCCTGC ATGACAAGAA GGGCGTTGTG GTTGGCAAGG AGCTGACCAT CGAGGACGTG AAGGTGCTGC GCCAAGTCAA GGATGGCATT ACTGACTACC AGAGCCACAG TGACAGCGAC GTCGTTGTGC TGGTCGATAA GCGCCAGGAT CAGGAGCTGA TTGACTCGTG GCGCGCACGT GAGTTTGTGA ATCGTGTGCA GCAGCTGCGT AAGAAGGCGA AGCTGGTGGT GACAGACGAG GTGGATGTGT ACTTCGAGGC AGAGGAGGCG GATCTAACGG CGTCCATCCT CAACTCGAAG AACCAGATTA ATCAGACACT GAGGGGTGTG TGGGCGACGA TGGACCAGAA GCCTAGCGAC GCCGAGCTCG TCGCGGAAGA GGACAACAGC ATCTCGGGGG TGACGGTGCG CCTCGTGTTC ACCAAGCCCA AGGCCTAA
Details for LetaA.18708.a.B3.GE42822
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME MLYQQIKPLL PANEQVDSVH YLMIPDEDAS LDDPELERAM SRMMNIVDLV RVLRDQMVIP IKRPVRQVVI VHPDQQYIDD VRKVADYIKD EVNAFEIVMS SGEDYVETKL DANFEVLGKK YRKEMPAIRK GIQAMTQEEV AKFLHDKKGV VVGKELTIED VKVLRQVKDG ITDYQSHSDS DVVVLVDKRQ DQELIDSWRA REFVNRVQQL RKKAKLVVTD EVDVYFEAEE ADLTASILNX KNQINQTLRG VWATMDQKPS DAELVAEEDN SISGVTVXXX XTKPK
Validated NT Sequence
gccttgggct tggtnnnnnn nnnnnncacc gtcacccccg agatgctgtt gtcctcttcc gcgacgagct cggcgtcgct aggcttctgg tccatcgtcg cccacacacc cctcagtgtc tgattaatct ggttcttnnn gttgaggatg gacgccgtta gatccgcctc ctctgcctcg aagtacacat ccacctcgtc tgtcaccacc agcttcgcct tcttacgcag ctgctgcaca cgattcacaa actcacgtgc gcgccacgag tcaatcagct cctgatcctg gcgcttatcg accagcacaa cgacgtcgct gtcactgtgg ctctggtagt cagtaatgcc atccttgact tggcgcagca ccttcacgtc ctcgatggtc agctccttgc caaccacaac gcccttcttg tcatgcagga acttggccac ctcctcctgc gtcatagcct ggataccctt gcggatcgca ggcatctcct tgcggtactt cttgccaagt acctcaaagt ttgcgtccag tttcgtctcc acgtaatctt cgccactcga catgacgatt tcgaatgcgt tcacctcatc cttaatgtag tccgctacct tgcgcacgtc gtcaatgtat tgctggtctg ggtgaacaat gacaacctga cgcaccggcc gcttaatcgg aatgaccatc tgatcacgca acacacgtac caaatccaca atgttcatca tgcgtgacat ggcacgctcc aactccgggt cgtccaagga ggcgtcctcg tcggggatca tgaggtagtg cacggagtcc acttgctcat tcgccgggag gaggggcttg atttgctggt aaagcatttc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHEM LYQQIKPLLP ANEQVDSVHY LMIPDEDASL DDPELERAMS RMMNIVDLVR VLRDQMVIPI KRPVRQVVIV HPDQQYIDDV RKVADYIKDE VNAFEIVMSS GEDYVETKLD ANFEVLGKKY RKEMPAIRKG IQAMTQEEVA KFLHDKKGVV VGKELTIEDV KVLRQVKDGI TDYQSHSDSD VVVLVDKRQD QELIDSWRAR EFVNRVQQLR KKAKLVVTDE VDVYFEAEEA DLTASILNSK NQINQTLRGV WATMDQKPSD AELVAEEDNS ISGVTVRLVF TKPKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatgc tttaccagca aatcaagccc ctcctcccgg cgaatgagca agtggactcc gtgcactacc tcatgatccc cgacgaggac gcctccttgg acgacccgga gttggagcgt gccatgtcac gcatgatgaa cattgtggat ttggtacgtg tgttgcgtga tcagatggtc attccgatta agcggccggt gcgtcaggtt gtcattgttc acccagacca gcaatacatt gacgacgtgc gcaaggtagc ggactacatt aaggatgagg tgaacgcatt cgaaatcgtc atgtcgagtg gcgaagatta cgtggagacg aaactggacg caaactttga ggtacttggc aagaagtacc gcaaggagat gcctgcgatc cgcaagggta tccaggctat gacgcaggag gaggtggcca agttcctgca tgacaagaag ggcgttgtgg ttggcaagga gctgaccatc gaggacgtga aggtgctgcg ccaagtcaag gatggcatta ctgactacca gagccacagt gacagcgacg tcgttgtgct ggtcgataag cgccaggatc aggagctgat tgactcgtgg cgcgcacgtg agtttgtgaa tcgtgtgcag cagctgcgta agaaggcgaa gctggtggtg acagacgagg tggatgtgta cttcgaggca gaggaggcgg atctaacggc gtccatcctc aactcgaaga accagattaa tcagacactg aggggtgtgt gggcgacgat ggaccagaag cctagcgacg ccgagctcgt cgcggaagag gacaacagca tctcgggggt gacggtgcgc ctcgtgttca ccaagcccaa ggcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat