LetaA.18664.b

Protein kinase, putative (KKT2)

CENTER ID: LetaA.18664.b
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.18664.b.B2.GE42821 PHoranyi kinase domain truncation 52 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP36.5470

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHFCSSLSR TPPRGGVVSM PRDLSQTPAI SRLGSTVKTP HIQKCVVDQT EDDDHPLEHM TVHFEEEDLR LVTTGLLGKG GFGKVFDAVS NSGESYALKV SSKRMSENDW KRLKEEVTLM SHFSRHPNIV KFYAAGRDQD RAYVVMERCA GMSLHDVIAS RSLDVPEILW IGWALVNTIS YIHSKGCIHR DLKPQNLLFD NNGNLKITDF GLSSRISEAH PRKTVAGTAM YMAPEMATEV YKRLTKNSDA PSLSYGKEVD TWSIGVVLYV LLTRMNPYLE AIEHKGMRQL DKEHKSLALF NAVAGAAWSW PKEWRGDPQL CRLVERVLHC EPSRRATLME VLEDSVWNRR PLSCPLSLLQ KLNLLEPSPA SGLPLNHLAE NLQFRPKRSA EAVLREGRER IEATEQRGRA QLELEYYESF NVLWSLLTLG RAEGDARADI LQSEEVQRGK LRNQSMSRRS ARGRCGSVSL VSEAADGETV APRTSRSVRR SVSLTEEERG RIVRSSPVQY AVVYPGRDTA TRWNLRAVVS LPRDMTDEIE REFKCMNGHV MTKLTSMPHG YNGFDCNVCD RGILKITGES PAFRCYKCDY DVCMKCAYSG KFKDVNFVCV TCAKRFTSTA KLQGHSLRCR GPSESPSPRR SSRMNTMLWE EPKRPSLLEV QLPEAPQSEP KLGASRCRSG RPTYNRTSTG GRISIGDPNA HSVVDFDAMV AEHREADFPE VSARMSITGR ESSQKRVRAG SQRGRPSTSS SGSLSLDLPP QVQLPSKASC TQVQPRSSAE LRDIMEEVRQ QKQILPRDPL LSGPATPPQY NSNGEIIGIA ARRRAESLEV ARAEVITIRA EVADRPRELQ HQPRVPRSAS STRVEKGLRS PHKRCREEWQ HPVHTSSSSG TAKRAAVEEQ IVKHTAVPPQ VPRGQAQQPR APSVSGHTAQ GGPPLPRRGP AAPSPAAALK AHLSALQVPA AIPPKNFSSI LQSRYSMTNA MAPTHNTSTR LTDGAGTATA PLGQRGVPTY SHALSRPNGA FLALPREERN RQQFLDDFLS GGWVRFYSFT NEDTVVMYYS LQPGRYGAMF PTEAGVGTAV LDVYSKLVLY VPCMNNESTN RNQPHPHVQT FYDEEARILT LPEAQRYLGG VLRCITGFVD EFSRLKAEGL TPAAVHAAYI HHRSMTHVPR DTKFVYIRKV FPDPSGSFTL FRLSNLRSQV VCNAMVDIRW QSDRRHNVGQ KYYINADGTA EPFLVDNTGI LSQLETVLNN NFRR
NT Sequence
atgtcacact tttgcagctc attgtcgagg acaccacctc gtggtggtgt ggtgtctatg ccgcgcgatt tgtcgcagac ccctgccatc tctcgacttg gaagcacagt gaagacaccg cacattcaaa agtgtgtcgt tgaccaaaca gaggacgatg atcatccact ggagcacatg acggtgcatt tcgaggagga ggaccttaga cttgttacca ctgggctgct tggaaaaggt ggatttggaa aggtatttga tgctgtttcc aacagtggtg agtcctacgc actgaaggtg tcatcgaaac gcatgagcga aaacgactgg aagcgattga aggaggaggt gacactcatg agccatttct cccgtcatcc caacattgtc aaattctatg ccgctggtag ggaccaagat cgtgcctacg tagtaatgga gcgatgtgca ggcatgtcac ttcacgatgt catagctagc aggagtcttg atgtgccgga gattttgtgg attgggtggg ctctggtgaa cactatctcc tacattcatt ccaaaggctg cattcaccgc gacttgaagc cacagaatct attgtttgat aacaacggta atttgaagat aacagatttt ggactttcta gccgcatatc agaggcacat cctcgcaaga cagttgcggg tacagcaatg tacatggctc ctgaaatggc aactgaggtt tacaagcgat tgacaaagaa ctcggacgcc ccttcgttga gctacgggaa ggaggtggac acgtggagta ttggtgtggt tctgtatgta ctcttgacac gtatgaatcc gtatctcgaa gcgatagaac acaaaggcat gcgtcaactg gacaaggagc acaaatccct tgccctcttt aacgccgtag cgggtgctgc gtggagttgg ccaaaagagt ggaggggaga tccacagctc tgccgacttg tggagcgcgt gttgcactgc gagccgtcgc gacgcgccac actgatggag gtgctggaag actctgtgtg gaaccgccgg ccactgtctt gcccactttc gctgctccag aagctcaact tgctagagcc ctcgccagca agtggcctgc ctctgaatca ccttgccgag aacttacagt tccgtccgaa gcgctcggcg gaggcggtgt tgcgcgaagg gcgagagcgt attgaagcca ctgagcaacg cggccgtgcg cagttggagc tggagtacta tgaaagcttc aatgtcctct ggagcctcct taccctaggc cgagcagaag gggacgctag agctgacatc ctccagtccg aggaggtgca gcgaggaaag ctgcgcaatc agtctatgtc tcgacggtct gctcgtggga ggtgtggcag cgtctcactg gtgtcggagg ccgctgatgg cgagacagtt gcccctcgca catctcgatc agtccgtcgc agcgtcagct tgactgagga agagcgggga agaattgtgc gttctagccc cgtccagtac gctgtggtgt acccagggcg cgacacagcg actcggtgga accttcgcgc cgtggtatca ctgccacgag acatgacgga cgagattgag cgcgagttca agtgcatgaa cgggcacgta atgacaaagc tgacctcgat gccgcatggc tacaacggct tcgactgcaa tgtgtgcgat cgcggcattc ttaagatcac gggcgagtca ccggcctttc gatgctacaa gtgtgactac gatgtttgta tgaagtgtgc gtactctggc aagttcaaag acgtcaactt tgtctgtgtc acgtgcgcga agcggttcac ctccaccgca aagctgcagg ggcattcctt acgctgtcgc ggcccgagcg aaagtccttc accgcggcga tcgtcgcgta tgaacacgat gctctgggag gagccaaaga gaccaagcct gctggaggta cagctcccag aagcccctca gagcgagccg aagctgggtg ccagccgctg ccgctccgga cgccccacgt acaaccgcac atcgaccggc ggccgcatca gcattggaga cccgaatgcg cacagtgtgg tcgacttcga tgcaatggtg gccgaacacc gcgaggctga ctttcccgag gtgagcgcac gcatgtccat cactgggcgc gaatcttctc agaaacgggt gcgtgcgggt agccagcgtg gtcgaccatc gacctcgtct agcggcagcc tttcactaga tctgccgccg caggtacagt taccaagcaa agcgtcatgc acacaagtgc agccgcgcag ctccgccgag ctgcgtgata tcatggagga ggtgaggcag cagaagcaga tattgccccg tgatcccctc ttgtccgggc cggccacacc gccgcagtat aacagcaacg gcgagatcat tggtattgct gctcgtcgcc gcgcagaaag cctagaggtg gcgcgcgcgg aagtcatcac gatccgcgcc gaggttgcgg accgaccgcg tgagctgcaa catcagccac gtgtgcctcg cagcgcctcc tcaacgaggg tggagaaggg gcttcggagc ccgcacaagc gttgtcgaga ggagtggcag catcccgtgc atacgtcgtc atcatcgggt acagcaaagc gagccgccgt ggaggagcaa atcgtgaagc atactgccgt gccgcctcag gtgccacgag ggcaagcgca gcagccacga gccccttccg ttagcgggca caccgcacag ggcggtcctc cactgccccg ccgcggccca gccgcaccat ctcctgcagc cgctttgaag gcccacctga gtgccttgca ggtccccgca gccattcctc ccaagaactt ttcctccatt ttgcagtcgc gatactccat gacgaacgcg atggcgccca cacacaatac ttcgacaaga ctcactgacg gagcaggaac cgcgaccgcc cctcttggtc agaggggagt accgacatac agccatgcgc taagccggcc caacggtgcc tttttggcgt tgccgcgcga ggagcgcaat cgccagcaat tcttggacga cttccttagt ggtgggtggg tgcgtttcta ctctttcaca aatgaggaca ccgtcgtcat gtactactca ctgcagcctg gtcgctacgg ggccatgttt cccaccgagg caggcgtcgg cactgctgtg ttagacgtgt actcgaagct ggtcctctat gtgccgtgta tgaacaacga gagcacgaac cgcaatcaac ctcacccaca tgtgcaaacg ttctacgacg aggaggcgcg cattcttacc ctgcctgagg cgcagcggta cctcggcggt gtgctacgtt gcattacggg atttgtagac gagtttagcc gcttgaaagc tgaggggctt actccagcgg cggtgcacgc tgcctacatt caccaccgta gcatgactca cgtgcctcgg gatacgaagt ttgtgtacat ccgaaaagta ttccccgacc cgtctgggtc tttcacgctg ttccgcctgt caaacttgcg ttcgcaagtt gtttgcaacg ctatggtgga cattcgctgg cagagtgacc gacgccacaa cgttggccaa aagtattaca tcaacgcgga cggcactgct gagccttttc tcgtcgacaa cactgggatt ctctcgcagc tagagacggt cctgaataac aatttcagga gatga
Details for LetaA.18664.b.B2.GE42821
HARVESTED ON: data unavailable
SEQUENCED ON: 1/15/2019
EXPECTED MW: 34kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 65
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMX DDXXXXXXXV HXXEEXLXLV XXGLLGKGXX GKVFDAVXXX XESXXXXXXX XLMSEXXWKR LKEEVTLMXH FSRHPNIVKF XAXGRDXXXA YXXMEXXXXX SLXXVIASRX LDAPEXXWXX WALVXXISYI HSKGCIHRDX XPQNLLFXXN GXXKITDFXL SXXISEAHPR KTXXGTXXXM APEMAXEVXX RLTKNSDAPS LSYGKEVDTW SXXVVLYVLX TRXXPYXXAI EHKGXRXXXK EHKSXXXFNA VAGAXWXXXK XXRGXXXXXR XVEXVLXXXP SRRA
Validated NT Sequence
tggcgcgtcg cgacggctnn nnnnncaana cnnnctccac nnnncggcnn nnntnnnnnt ctcccctcnn nnntttnnnn nnnntccacn nngcncccgc tacggcgtta aagannnnnn nngatttgtg ctccttgcnc ngnngacgnn ngcctttgtg ttctatcgct tnnngatacg gannnnnacg tgtnnngagt acatanagaa ccacnnnnat actccacgtg tcnacctcct tcccgtagct caacgaaggg gcgtccgagt tctttgtcaa tcgctnnnaa acctcnnnng ccatttcagg agccatgtnc annnctgtac ccnnnnctgt cttgcgagga tgtgcctctg atatgnnnct agaaagnnca aaatctgtta tcttcnnnnt accgttnnnn tcaaacaata gattctgtgg ctnnaagtcg cggtgaatgc agcctttgga atgaatgtag gagatnnnnt tcaccagagc ccaccnaanc cannnnatct ccggcgcatc aagnntcctg ctagctatga cannntnaag tgacangnct nnnnnnngct ccatnnnnnc gtaggcnnna nnnnggtccc taccannggc nnngaatttg acaatgttgg gatgacggga gaaatgnnnc atgagtgtca cctcctcctt caancgcttc cagnnntttt cgctcatgag ttnnnnnnac anntncannn nntaggactc ancnnngnnn nnaacagcat caaatacctt tccanancna ccttttccaa gcagcccagn nntaacaagn nnaagnnnct cctccnnnaa atgcacnnnn nngtnnnnna nnnnntgatc atcnnncata tggtggtggt ggtggtgagc catggtata
Expected Protein Sequence
MAHHHHHHDD DHPLEHMTVH FEEEDLRLVT TGLLGKGGFG KVFDAVSNSG ESYALKVSSK RMSENDWKRL KEEVTLMSHF SRHPNIVKFY AAGRDQDRAY VVMERCAGMS LHDVIASRSL DVPEILWIGW ALVNTISYIH SKGCIHRDLK PQNLLFDNNG NLKITDFGLS SRISEAHPRK TVAGTAMYMA PEMATEVYKR LTKNSDAPSL SYGKEVDTWS IGVVLYVLLT RMNPYLEAIE HKGMRQLDKE HKSLALFNAV AGAAWSWPKE WRGDPQLCRL VERVLHCEPS RRATLMEVLE DSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgatg atcatccact ggagcacatg acggtgcatt tcgaggagga ggaccttaga cttgttacca ctgggctgct tggaaaaggt ggatttggaa aggtatttga tgctgtttcc aacagtggtg agtcctacgc actgaaggtg tcatcgaaac gcatgagcga aaacgactgg aagcgattga aggaggaggt gacactcatg agccatttct cccgtcatcc caacattgtc aaattctatg ccgctggtag ggaccaagat cgtgcctacg tagtaatgga gcgatgtgca ggcatgtcac ttcacgatgt catagctagc aggagtcttg atgtgccgga gattttgtgg attgggtggg ctctggtgaa cactatctcc tacattcatt ccaaaggctg cattcaccgc gacttgaagc cacagaatct attgtttgat aacaacggta atttgaagat aacagatttt ggactttcta gccgcatatc agaggcacat cctcgcaaga cagttgcggg tacagcaatg tacatggctc ctgaaatggc aactgaggtt tacaagcgat tgacaaagaa ctcggacgcc ccttcgttga gctacgggaa ggaggtggac acgtggagta ttggtgtggt tctgtatgta ctcttgacac gtatgaatcc gtatctcgaa gcgatagaac acaaaggcat gcgtcaactg gacaaggagc acaaatccct tgccctcttt aacgccgtag cgggtgctgc gtggagttgg ccaaaagagt ggaggggaga tccacagctc tgccgacttg tggagcgcgt gttgcactgc gagccgtcgc gacgcgccac actgatggag gtgctggaag actctgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat