LetaA.18236.a

KREL2

CENTER ID: LetaA.18236.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.18236.a.B1.GE40702 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LtaP20.1670

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRRCLLARL VRRSPVLFSD EKEALFERYS EIENAHSRRI EALKDAGLFN DEWIATEKVH GANFGIYSTE HGKIIQYAKR SGIMPPHEHF FGYHVLIPDL ARYIKQGREL LTEQLGVSPT TVIMNGELFG GKYDHPSVSK RRQSVMVAGK SRTITAVQNN AFPQYSPDLH FYAFDIKYRM TEEEEYRTMT YDQALAIFEK IPGLLYARAI IRGPLSKVVA FDVENFATTI PSLVGMGDYP LKGNWAEGLV VKHCRRGESG FDAKGVTIMK FKCTAFQEIS TDRRQGPRVD ALEEVRRQAI QASGVQLPDM ESVIRDSEVR KAAHHLLNHV CENRLQSVLS KIGTDPFETQ SMTPAELSTL LAKDALKDFL KEADAKIVNT PLLVRREMTR YVLFEARKYV ASRWKGIVGQ QKEADRKS
NT Sequence
ATGTTTCGGC GATGCCTGCT TGCACGGCTC GTACGGCGCT CGCCAGTTCT CTTCTCAGAC GAAAAGGAAG CGCTGTTTGA GCGCTATAGT GAGATTGAAA ACGCCCACTC GCGACGCATT GAAGCACTGA AGGACGCGGG TCTCTTCAAT GATGAGTGGA TCGCTACGGA GAAGGTACAT GGTGCCAACT TCGGGATATA CTCAACAGAG CATGGGAAGA TTATTCAGTA CGCGAAGCGG AGCGGCATCA TGCCGCCACA CGAACACTTT TTTGGCTACC ACGTTCTCAT ACCTGATCTC GCAAGGTATA TCAAACAAGG GCGAGAACTC CTTACGGAAC AGCTCGGCGT GTCACCCACC ACCGTAATCA TGAACGGGGA GCTCTTTGGT GGCAAGTATG ACCATCCGAG CGTGTCAAAG CGGCGGCAGA GCGTGATGGT GGCCGGCAAG TCACGCACCA TCACAGCCGT GCAGAACAAT GCCTTCCCAC AGTACTCCCC AGACCTGCAT TTCTACGCCT TTGACATCAA GTACCGCATG ACCGAAGAGG AAGAGTACCG CACCATGACG TATGACCAAG CCTTGGCCAT CTTCGAGAAA ATTCCGGGCC TGCTCTACGC GAGGGCTATC ATTCGAGGGC CGCTCAGCAA GGTGGTTGCG TTCGATGTGG AGAACTTTGC CACGACAATT CCATCGCTGG TGGGCATGGG CGACTACCCG CTCAAGGGTA ACTGGGCAGA GGGTCTCGTC GTAAAGCACT GTCGTCGTGG AGAGTCCGGC TTTGACGCAA AAGGCGTCAC TATCATGAAG TTCAAGTGTA CCGCCTTCCA GGAGATCTCG ACTGATCGCC GCCAGGGGCC TCGCGTAGAC GCGCTGGAGG AGGTACGGCG GCAAGCCATA CAAGCCTCTG GCGTCCAGCT GCCGGACATG GAGAGCGTCA TCCGCGACTC CGAGGTGCGC AAAGCGGCAC ATCACCTGCT CAACCACGTA TGTGAAAATC GCCTACAGAG TGTCCTCTCC AAGATCGGTA CAGACCCATT CGAGACGCAG TCTATGACCC CTGCAGAGCT CTCTACGCTG CTCGCAAAGG ATGCGCTGAA GGACTTCTTG AAGGAGGCGG ATGCAAAGAT CGTCAACACA CCGCTACTCG TGCGCCGGGA GATGACCCGC TACGTTCTCT TCGAGGCACG CAAGTACGTT GCCAGTAGAT GGAAAGGCAT CGTCGGGCAG CAAAAGGAGG CGGACAGAAA ATCGTGA
Details for LetaA.18236.a.B1.GE40702
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF RRCLLARLVR RSPVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KIIQYAKRSG IMPSHEHFFG YHVLIPDLAR YIKQGRELLT EQLGVSPTTV IMNGELFGGK YDHPSVSKRR QSVMVAGKSR TITAVQNNAF PQYSPDLHFY AFDIKYRMTE EEEYRTMTYD QALAIFEKIP GLLYARAIIR GPLSKVVAFD VENFATTIPS LVGMGDYPLK GNWAEGLVVK HCRRGESGFD AKGVTIMKFK CTAFQEISTD RRQGPRVDAL EEVRRQAIQA SGVQLPDMES VIRDSEVRKA AHHLLNHVCE NRLQSVLSKI GTDPFETQSM TPAELSTLLA KDALKDFLKE ADAKIVNTPL LVRREMTRYV LFEARKYVAS RWEGIVGQQ
Validated NT Sequence
atggctcacc accaccacca ccatatgttt cggcgatgcc tgcttgcacg gctcgtacgg cgctcgccag ttctcttctc agacgaaaag gaagcgctgt ttgagcgcta tagtgagatt gaaaacgccc actcgcgacg cattgaagca ctgaaggacg cgggtctctt caatgatgag tggatcgcta cggagaaggt acatggtgcc aacttcggga tatactcaac agagcatggg aagattattc agtacgcgaa gcggagcggc atcatgccgt cacacgaaca cttttttggc taccacgttc tcatacctga tctcgcaagg tatatcaaac aagggcgaga actccttacg gaacagctcg gcgtgtcacc caccaccgta atcatgaacg gggagctctt tggtggcaag tatgaccatc cgagcgtgtc aaagcggcgg cagagcgtga tggtggccgg caagtcacgc accatcacag ccgtgcagaa caatgccttc ccacagtact ccccagacct gcatttctac gcctttgaca tcaagtaccg catgaccgaa gaggaagagt atcgcaccat gacgtatgac caagccttgg ccatcttcga gaaaattccg ggcctgctct acgcgagggc tatcattcga gggccgctca gcaaggtggt tgcgttcgat gtggagaact ttgctacgac aattccatcg ctggtgggca tgggcgacta cccgctcaag ggtaactggg cagagggtct cgtcgtaaag cactgtcgtc gtggagagtc cggctttgac gcaaaaggcg tcacaatcat gaagttcaag tgtaccgcct tccaggagat ctcgactgat cgccgccagg ggcctcgcgt agacgcgctg gaggaggtac ggcggcaagc catacaagcc tctggcgtcc agctgccgga catggagagc gtcatccgcg actccgaggt gcgcaaagcg gcacatcacc tgctcaacca cgtatgtgaa aatcgcctac agagtgtcct ctccaagatc ggtacagacc cattcgagac gcagtctatg acccctgcag agctctctac gctgctcgca aaggatgcgc tgaaggactt cttgaaggag gcggatgcaa agatcgtcaa cacaccgcta ctcgtgcgcc gggagatgac ccgctacgtt ctcttcgagg cacgcaagta cgtcgccagt agatgggaag gcatcgtcgg gcagcaaa
Expected Protein Sequence
MAHHHHHHMF RRCLLARLVR RSPVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KIIQYAKRSG IMPPHEHFFG YHVLIPDLAR YIKQGRELLT EQLGVSPTTV IMNGELFGGK YDHPSVSKRR QSVMVAGKSR TITAVQNNAF PQYSPDLHFY AFDIKYRMTE EEEYRTMTYD QALAIFEKIP GLLYARAIIR GPLSKVVAFD VENFATTIPS LVGMGDYPLK GNWAEGLVVK HCRRGESGFD AKGVTIMKFK CTAFQEISTD RRQGPRVDAL EEVRRQAIQA SGVQLPDMES VIRDSEVRKA AHHLLNHVCE NRLQSVLSKI GTDPFETQSM TPAELSTLLA KDALKDFLKE ADAKIVNTPL LVRREMTRYV LFEARKYVAS RWKGIVGQQK EADRKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc ggcgatgcct gcttgcacgg ctcgtacggc gctcgccagt tctcttctca gacgaaaagg aagcgctgtt tgagcgctat agtgagattg aaaacgccca ctcgcgacgc attgaagcac tgaaggacgc gggtctcttc aatgatgagt ggatcgctac ggagaaggta catggtgcca acttcgggat atactcaaca gagcatggga agattattca gtacgcgaag cggagcggca tcatgccgcc acacgaacac ttttttggct accacgttct catacctgat ctcgcaaggt atatcaaaca agggcgagaa ctccttacgg aacagctcgg cgtgtcaccc accaccgtaa tcatgaacgg ggagctcttt ggtggcaagt atgaccatcc gagcgtgtca aagcggcggc agagcgtgat ggtggccggc aagtcacgca ccatcacagc cgtgcagaac aatgccttcc cacagtactc cccagacctg catttctacg cctttgacat caagtaccgc atgaccgaag aggaagagta ccgcaccatg acgtatgacc aagccttggc catcttcgag aaaattccgg gcctgctcta cgcgagggct atcattcgag ggccgctcag caaggtggtt gcgttcgatg tggagaactt tgccacgaca attccatcgc tggtgggcat gggcgactac ccgctcaagg gtaactgggc agagggtctc gtcgtaaagc actgtcgtcg tggagagtcc ggctttgacg caaaaggcgt cactatcatg aagttcaagt gtaccgcctt ccaggagatc tcgactgatc gccgccaggg gcctcgcgta gacgcgctgg aggaggtacg gcggcaagcc atacaagcct ctggcgtcca gctgccggac atggagagcg tcatccgcga ctccgaggtg cgcaaagcgg cacatcacct gctcaaccac gtatgtgaaa atcgcctaca gagtgtcctc tccaagatcg gtacagaccc attcgagacg cagtctatga cccctgcaga gctctctacg ctgctcgcaa aggatgcgct gaaggacttc ttgaaggagg cggatgcaaa gatcgtcaac acaccgctac tcgtgcgccg ggagatgacc cgctacgttc tcttcgaggc acgcaagtac gttgccagta gatggaaagg catcgtcggg cagcaaaagg aggcggacag aaaatcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at