LetaA.18222.b

Glutamyl-tRNA synthetase, putative

CENTER ID: LetaA.18222.b
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.18222.b.B1.GE42748 full length 1 594
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP30.3280

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRFLPIHML VNIHLKALTL SNTYFTGALQ REENEKLQLS NAEKGKVVTR FPPEASGFLH IGHAKAALIN RMLADAYGGK MLFRFDDTNP SKEKEHFEAA IEEDLKTLGV RYDVGPTYSS DYFDLMFEKA EDMIKRGLAY CDKTPREEMQ KCRFDGIPTK YRDASVEENM RLWKEMKKGS EEGQITCLRA KVSIDDPNKA MRDPVMYRVS MVPHARHGTK QVAYPTYDFC CPLIDSVEGV THALRTNEYH DRNAQYYWFC EAMGIRKPII EDFSRLNMEY SVMSKRMLTK LVDTHVVDGW DDPRLPTVRA LVRRGMKIEA LRQFVAEQGM SKSVNFMEWS KIWFFNTQIL DPTSPRYTVV SNTLKVRCSV EGQRHMEAEK KPLHKKVPCM GEKTYYKSDV IFLDAEDVAL IKDGDEVTLM DWGNAYVKDI KRPSADALPT EATIVLHPEG DVKKTKFKLT WVPESDNAVI LTLNEYDNLL TKKKPDPEES IDDIIASVSR YSQEVIGEEA LSVLKKGDII QLERRGYYIV DDDKAVKKVL ISIPDGRDKV NHLSAKAQYL KTLPKKSSAA DELAAKRAAK AAKKAAQKAA AKSS
NT Sequence
ATGAGGAGAT TTTTACCTAT CCACATGTTA GTCAATATAC ACTTGAAGGC TCTAACGCTG AGCAACACCT ACTTTACAGG AGCTCTGCAA CGTGAGGAAA ATGAGAAGCT GCAGCTTTCT AATGCTGAGA AGGGCAAGGT GGTGACGCGC TTTCCCCCAG AGGCTAGCGG GTTCTTGCAT ATTGGCCACG CGAAGGCTGC GCTGATTAAC CGCATGCTGG CAGACGCGTA TGGTGGAAAG ATGCTCTTCC GCTTTGATGA CACAAACCCC TCCAAAGAAA AGGAGCACTT CGAGGCCGCC ATCGAGGAGG ATCTTAAGAC ACTGGGTGTG CGGTATGATG TTGGTCCAAC ATACTCGTCG GACTACTTTG ATTTGATGTT TGAGAAGGCG GAGGACATGA TCAAGCGTGG CTTGGCGTAC TGTGATAAGA CACCGCGGGA GGAGATGCAA AAGTGCCGCT TTGACGGTAT TCCGACGAAG TACCGCGATG CGTCGGTCGA GGAGAACATG CGCCTGTGGA AGGAAATGAA GAAGGGCAGT GAGGAAGGCC AGATCACATG CCTTCGTGCC AAGGTGTCCA TTGACGATCC CAATAAGGCG ATGCGGGACC CCGTCATGTA TCGCGTGAGC ATGGTCCCGC ACGCGCGCCA CGGCACGAAG CAGGTGGCGT ACCCGACATA TGACTTTTGC TGTCCTCTCA TCGACTCGGT GGAGGGTGTA ACCCATGCTC TTCGCACAAA TGAGTACCAT GACCGGAATG CTCAGTATTA CTGGTTCTGC GAAGCTATGG GGATCCGCAA GCCCATTATT GAGGACTTCT CGCGGCTCAA TATGGAGTAC TCGGTAATGT CAAAGCGAAT GCTGACAAAG TTGGTGGACA CGCACGTGGT GGATGGATGG GATGACCCGC GGCTTCCAAC GGTGCGCGCA CTGGTGCGCC GTGGTATGAA GATTGAGGCA CTTCGTCAGT TTGTGGCGGA GCAAGGCATG TCCAAGAGCG TGAACTTCAT GGAGTGGTCG AAGATCTGGT TCTTCAATAC ACAGATTCTC GACCCCACAA GCCCCCGATA CACAGTGGTG TCGAACACAC TGAAGGTCCG CTGCAGTGTG GAAGGGCAGA GGCACATGGA GGCTGAAAAG AAGCCGCTCC ACAAGAAGGT TCCCTGCATG GGCGAGAAGA CGTACTACAA GTCAGATGTT ATCTTTTTGG ACGCTGAGGA TGTGGCGCTC ATCAAAGATG GTGATGAGGT GACACTGATG GACTGGGGCA ACGCGTATGT CAAGGACATA AAGCGTCCGA GCGCTGATGC CCTGCCTACT GAGGCTACGA TCGTTTTGCA CCCAGAAGGG GATGTCAAGA AGACGAAATT CAAGCTCACG TGGGTGCCTG AAAGTGATAA TGCCGTGATT TTGACTCTGA ACGAGTACGA TAATCTCTTG ACCAAGAAGA AACCAGACCC GGAGGAGAGC ATTGATGATA TCATTGCTTC CGTTTCCCGC TACTCGCAGG AGGTTATTGG CGAGGAGGCG CTGTCGGTGC TGAAGAAGGG TGACATTATC CAGCTGGAGC GCCGCGGCTA CTACATCGTC GACGACGACA AGGCGGTCAA GAAGGTGCTC ATCTCAATTC CGGATGGACG CGATAAGGTG AACCACCTTT CTGCCAAGGC GCAGTACCTC AAGACACTGC CCAAGAAGTC ATCCGCCGCT GATGAGCTTG CAGCCAAGCG CGCAGCCAAG GCAGCGAAGA AGGCAGCTCA GAAGGCAGCG GCGAAGAGTA GCTAG
Details for LetaA.18222.b.B1.GE42748
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR RFLPIHMLVN IHLKALTLSN TYFTGALQRE ENEKLQLSNA EKGKVVTRFP PEASGFLHIG HAKAALINRM LADAYGGKML FRFDDTNPSK EKEHFEAAIE EDLKTLGVRY DVGPTYSSDY FDLMFEKAED MIKRGLAYCD KTPREEMQKC RFDGIPTKYR DASVEENMRL WKEMKKGSEE GQITCLRAKV SIDDPNKAMR DPVMYRVSMV PHARHGTKQV AYPTYDFCCP LIDSVEGVTH ALRTNEYHDR NAQYYWFCEA MGIRKPIIED FSRLNMEYSV MSKRMLTKLV DTHVVDGWDD PRLPTVRALV RRGMKIEALR QFVAEQGMSK SVNFMEWSKI WFFNTQILDP TSPRYTVVSN TLKVRCSVEG QRHMEAEKKP LHKKVPCMGE KTYYKSDVIF LDAEDVALIK DGDEVTLMDW GNAYVKDIKR PSADALPTEA TIVLHPEGDV KKTKFKLTWV PESDNAVILT LNEYDNLLTK KKPDPEESID DIIASVSRYS QEVIGEEALS VLKKGDIIQL ERRGYYIVDD DKAVKKVLIS IPDGRDKVNH LSAKAQYLKT LPKKSSAADE LAAKRAAKAA KKAAQKAAAK SS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagga gatttttacc tatccacatg ttagtcaata tacacttgaa ggctctaacg ctgagcaaca cctactttac aggagctctg caacgtgagg aaaatgagaa gctgcagctt tctaatgctg agaagggcaa ggtggtgacg cgctttcccc cagaggctag cgggttcttg catattggcc acgcgaaggc tgcgctgatt aaccgcatgc tggcagacgc gtatggtgga aagatgctct tccgctttga tgacacaaac ccctccaaag aaaaggagca cttcgaggcc gccatcgagg aggatcttaa gacactgggt gtgcggtatg atgttggtcc aacatactcg tcggactact ttgatttgat gtttgagaag gcggaggaca tgatcaagcg tggcttggcg tactgtgata agacaccgcg ggaggagatg caaaagtgcc gctttgacgg tattccgacg aagtaccgcg atgcgtcggt cgaggagaac atgcgcctgt ggaaggaaat gaagaagggc agtgaggaag gccagatcac atgccttcgt gccaaggtgt ccattgacga tcccaataag gcgatgcggg accccgtcat gtatcgcgtg agcatggtcc cgcacgcgcg ccacggcacg aagcaggtgg cgtacccgac atatgacttt tgctgtcctc tcatcgactc ggtggagggt gtaacccatg ctcttcgcac aaatgagtac catgaccgga atgctcagta ttactggttc tgcgaagcta tggggatccg caagcccatt attgaggact tctcgcggct caatatggag tactcggtaa tgtcaaagcg aatgctgaca aagttggtgg acacgcacgt ggtggatgga tgggatgacc cgcggcttcc aacggtgcgc gcactggtgc gccgtggtat gaagattgag gcacttcgtc agtttgtggc ggagcaaggc atgtccaaga gcgtgaactt catggagtgg tcgaagatct ggttcttcaa tacacagatt ctcgacccca caagcccccg atacacagtg gtgtcgaaca cactgaaggt ccgctgcagt gtggaagggc agaggcacat ggaggctgaa aagaagccgc tccacaagaa ggttccctgc atgggcgaga agacgtacta caagtcagat gttatctttt tggacgctga ggatgtggcg ctcatcaaag atggtgatga ggtgacactg atggactggg gcaacgcgta tgtcaaggac ataaagcgtc cgagcgctga tgccctgcct actgaggcta cgatcgtttt gcacccagaa ggggatgtca agaagacgaa attcaagctc acgtgggtgc ctgaaagtga taatgccgtg attttgactc tgaacgagta cgataatctc ttgaccaaga agaaaccaga cccggaggag agcattgatg atatcattgc ttccgtttcc cgctactcgc aggaggttat tggcgaggag gcgctgtcgg tgctgaagaa gggtgacatt atccagctgg agcgccgcgg ctactacatc gtcgacgacg acaaggcggt caagaaggtg ctcatctcaa ttccggatgg acgcgataag gtgaaccacc tttctgccaa ggcgcagtac ctcaagacac tgcccaagaa gtcatccgcc gctgatgagc ttgcagccaa gcgcgcagcc aaggcagcga agaaggcagc tcagaaggca gcggcgaaga gtagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat