SSGCID: Target Page

LetaA.10252.a

Threonyl-tRNA synthetase, putative

CENTER ID:	LetaA.10252.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.10252.a.B2.GE43736	TEdwards Stma structure domain		287	788

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.10252.a.B2.PW38880	TEdwards Stma structure domain		287	788

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP35.1480

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGKKKAAEA TVDLVTLTEP DFWRQRVEIF EELWQKQQNR YESMKAPIKV SLPDGKVMDA ESWVTCPLDI AKRLSNSLPD KVIVARVNEV LWDLTRPLEA DCQLDLLDWD DKDAREVFWH SSSHVLGYAL ERIFDTKLSV GPALEEGGFF YEGLTNRAVS ESDYKAIESA MQELVKQKMP YQRLEVSKED ALRLFDYTAF KSKILASKVP DGGSCTVYRC GMLIDPCRGP HLPDTGRVKA FAVTKNSSSY FEGKAENEVL QRVYGISFPK NPMLAEWKKI QEEAAKRDHR VIGRQQNLFN FHEVSPGSAF WLPHGARIYN TLVEFLRKKY RRSGFQEVIS PNMYSSKLWM VSGHWEKYAD NMFCTKCEKE DFGLKPMNCP GHCIMFASQP HSYKELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQITD EMEGQMQFLS DVYGILGFKF YFYHSTRPAN KLGSDALWDQ SESYLRTALN RFCGIPEELP DPLHPDQRFH YDGKPDSVKK MKTLIKKAER SDDPNVWKGP TNSGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPNRFGL KYTLPSAEKD DATVAPTEKR RHADPAPTES STAEVEGEKK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQHVRDT MHDAGFYADV DNGASTLDKK IRNAEKARYN FILVVGQQEQ DATGVNIRAR GEKRLGNKSL EEAVQWFKEL TDTHNREY

NT Sequence

ATGTCTGGCA AGAAGAAGGC GGCGGAGGCG ACCGTGGACT TGGTCACCCT GACGGAGCCA GACTTCTGGA GGCAACGCGT TGAAATCTTT GAGGAGTTAT GGCAGAAGCA GCAGAACCGC TATGAGTCTA TGAAGGCCCC AATTAAGGTG AGCTTGCCGG ATGGTAAGGT GATGGACGCC GAGAGCTGGG TCACGTGTCC TCTGGACATC GCAAAGCGTC TCTCCAACTC GTTGCCAGAC AAGGTCATTG TCGCTCGCGT GAACGAGGTG CTATGGGACT TGACGCGACC GTTGGAGGCT GACTGCCAGC TGGATCTGCT GGACTGGGAC GACAAGGACG CGCGTGAGGT GTTCTGGCAC AGCTCTTCTC ACGTGCTCGG CTATGCGCTG GAGCGCATCT TTGACACGAA GCTTTCCGTC GGTCCGGCCC TGGAGGAGGG TGGCTTCTTT TACGAGGGCC TCACCAATCG CGCGGTCTCC GAATCCGACT ACAAGGCGAT TGAGTCAGCC ATGCAAGAGC TGGTGAAGCA GAAGATGCCA TACCAGCGTC TCGAAGTGTC GAAGGAGGAC GCCCTGAGAC TCTTCGACTA CACAGCGTTC AAGAGCAAGA TCCTGGCGAG CAAGGTGCCA GACGGGGGCT CGTGCACCGT GTATCGGTGC GGTATGCTGA TTGACCCGTG CCGTGGTCCC CATCTTCCAG ACACGGGCCG CGTGAAGGCT TTTGCTGTGA CTAAGAACTC CTCCTCTTAC TTTGAGGGCA AGGCCGAGAA CGAGGTGCTG CAGCGTGTGT ACGGTATTTC ATTTCCGAAG AACCCCATGC TGGCGGAGTG GAAGAAGATT CAGGAGGAGG CTGCGAAGCG TGACCACCGC GTGATTGGCC GCCAGCAGAA CCTCTTCAAC TTCCACGAGG TGTCGCCTGG CAGCGCGTTC TGGTTGCCGC ACGGCGCCCG CATCTACAAC ACTCTTGTTG AGTTTCTGCG CAAGAAGTAT CGGCGCTCTG GTTTCCAGGA GGTCATCTCA CCGAACATGT ACTCTTCCAA ACTGTGGATG GTGTCGGGGC ACTGGGAGAA GTACGCCGAC AACATGTTCT GCACCAAGTG CGAGAAGGAG GACTTTGGTC TGAAGCCGAT GAATTGCCCG GGCCACTGCA TCATGTTTGC GTCGCAGCCG CACTCATACA AGGAACTGCC GATTCGCTAC GCCGACTTTG GGGTACTGCA CCGGAACGAG CTGAGCGGTG CCTTGACGGG GTTGACCCGT GTGCGCCGCT TCCAGCAAGA CGATGCGCAC ATCTTCTGCC GCATGGACCA GATCACAGAT GAGATGGAGG GCCAGATGCA GTTCCTCAGC GATGTTTACG GCATTCTGGG TTTCAAGTTC TATTTTTACC ACTCTACCCG CCCCGCAAAC AAGCTCGGCT CTGACGCGCT CTGGGACCAG TCGGAGTCAT ATCTGCGCAC CGCCCTCAAT CGGTTCTGCG GCATCCCAGA GGAACTACCT GACCCGTTGC ACCCAGACCA GCGCTTTCAC TACGACGGCA AGCCAGACTC GGTGAAAAAG ATGAAGACAC TCATCAAGAA AGCGGAGCGC AGCGATGACC CGAATGTGTG GAAGGGCCCG ACCAACAGCG GCGATGGCTG GATTGAGAAC GCTGGTGATG GCGCCTTCTA CGGCCCAAAG ATTGACATCG TTGTCGAGGA CGCGCTGCGC CGTCGCCACC AGTGTGCCAC GATTCAGTTG GACTTCAATC TGCCCAATCG TTTTGGTCTC AAGTACACCC TCCCCTCGGC GGAGAAGGAC GATGCGACGG TAGCCCCAAC GGAGAAGCGG AGACATGCCG ACCCTGCCCC AACCGAGTCA TCGACAGCTG AGGTAGAGGG CGAGAAAAAG CCGAAGCCTG GCACGTATGA GGCTGCTGTG CGTGACCTTG GCATTGACCT CGAGCTGGAC GCCAACCAGG CACGCCCCGT CATGATCCAC CGCGCCATTC TCGGCTCCTT GGAGCGCTCG ATCGCCATTC TGTGCGAGCA CTTTGGCGGT GACTGGCCGT TCTGGCTGAG CCCCCGCCAA GTGATGGTGG TGCCTGTTTC GGCCTCCAAC TACGAGTATT CACAGCATGT TCGCGACACA ATGCACGATG CCGGTTTTTA CGCGGACGTT GACAACGGTG CCTCCACGTT GGACAAGAAG ATCCGCAACG CGGAGAAGGC GCGCTACAAC TTCATCCTCG TCGTTGGCCA ACAGGAGCAG GACGCGACCG GCGTCAACAT CCGTGCGCGC GGGGAGAAGA GGCTGGGTAA CAAGTCACTC GAAGAGGCAG TGCAGTGGTT CAAGGAGTTG ACGGACACAC ATAACCGGGA GTACTAG

Details for LetaA.10252.a.B2.GE43736

HARVESTED ON:	7/6/2020
SEQUENCED ON:	6/23/2020
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHRD HRVIGRQQNL FNFHEVSPGS AFWLPHGARI YNTLVEFLRK KYRRSGFQEV ISPNMYSSKL WMVSGHWEKY ADNMFCTKCE KEDFGLKPMN CPGHCIMFAS QPHSYKELPI RYADFGVLHR NELSGALTGL TRVRRFQQDD AHIFCRMDQI TDEMEGQMQF LSDVYGILGF KFYFYHSTRP ANKLGSDALW DQSESYLRTA LNRFCGIPEE LPDPLHPDQR FHYDGKPDSV KKMKTLIKKA ERSDDPNVWK GPTNSGDGWI ENAGDGAFYG PKIDIVVEDA LRRRHQCATI QLDFNLPNRF GLKYTLPSAE KDDATVAPTE KRRHADPAPT ESSTAEVEGE KKPKPGTYEA AVRDLGIDLE LDANQARPVM IHRAILGSLE RSIAILCEHF GGDWPFWLSP RQVMVVPVSA SNYEYSQHVR DTMHDAGFYA DVDNGASTLD KKIRNAEKAR YNFILVVGQQ EQDATGVNIR ARGEKRLGNK SLEEAVQWFK ELTDTHNREY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtgacc accgcgtgat tggccgccag cagaacctct tcaacttcca cgaggtgtcg cctggcagcg cgttctggtt gccgcacggc gcccgcatct acaacactct tgttgagttt ctgcgcaaga agtatcggcg ctctggtttc caggaggtca tctcaccgaa catgtactct tccaaactgt ggatggtgtc ggggcactgg gagaagtacg ccgacaacat gttctgcacc aagtgcgaga aggaggactt tggtctgaag ccgatgaatt gcccgggcca ctgcatcatg tttgcgtcgc agccgcactc atacaaggaa ctgccgattc gctacgccga ctttggggta ctgcaccgga acgagctgag cggtgccttg acggggttga cccgtgtgcg ccgcttccag caagacgatg cgcacatctt ctgccgcatg gaccagatca cagatgagat ggagggccag atgcagttcc tcagcgatgt ttacggcatt ctgggtttca agttctattt ttaccactct acccgccccg caaacaagct cggctctgac gcgctctggg accagtcgga gtcatatctg cgcaccgccc tcaatcggtt ctgcggcatc ccagaggaac tacctgaccc gttgcaccca gaccagcgct ttcactacga cggcaagcca gactcggtga aaaagatgaa gacactcatc aagaaagcgg agcgcagcga tgacccgaat gtgtggaagg gcccgaccaa cagcggcgat ggctggattg agaacgctgg tgatggcgcc ttctacggcc caaagattga catcgttgtc gaggacgcgc tgcgccgtcg ccaccagtgt gccacgattc agttggactt caatctgccc aatcgttttg gtctcaagta caccctcccc tcggcggaga aggacgatgc gacggtagcc ccaacggaga agcggagaca tgccgaccct gccccaaccg agtcatcgac agctgaggta gagggcgaga aaaagccgaa gcctggcacg tatgaggctg ctgtgcgtga ccttggcatt gacctcgagc tggacgccaa ccaggcacgc cccgtcatga tccaccgcgc cattctcggc tccttggagc gctcgatcgc cattctgtgc gagcactttg gcggtgactg gccgttctgg ctgagccccc gccaagtgat ggtggtgcct gtttcggcct ccaactacga gtattcacag catgttcgcg acacaatgca cgatgccggt ttttacgcgg acgttgacaa cggtgcctcc acgttggaca agaagatccg caacgcggag aaggcgcgct acaacttcat cctcgtcgtt ggccaacagg agcaggacgc gaccggcgtc aacatccgtg cgcgcgggga gaagaggctg ggtaacaagt cactcgaaga ggcagtgcag tggttcaagg agttgacgga cacacataac cgggagtact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for LetaA.10252.a.B2.PW38880

PURIFICATION DATe:	8/19/2020
CONCENTRATION:	25mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	24
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGTGACC ACCGCGTGAT TGGCCGCCAG CAGAACCTCT TCAACTTCCA CGAGGTGTCG CCTGGCAGCG CGTTCTGGTT GCCGCACGGC GCCCGCATCT ACAACACTCT TGTTGAGTTT CTGCGCAAGA AGTATCGGCG CTCTGGTTTC CAGGAGGTCA TCTCACCGAA CATGTACTCT TCCAAACTGT GGATGGTGTC GGGGCACTGG GAGAAGTACG CCGACAACAT GTTCTGCACC AAGTGCGAGA AGGAGGACTT TGGTCTGAAG CCGATGAATT GCCCGGGCCA CTGCATCATG TTTGCGTCGC AGCCGCACTC ATACAAGGAA CTGCCGATTC GCTACGCCGA CTTTGGGGTA CTGCACCGGA ACGAGCTGAG CGGTGCCTTG ACGGGGTTGA CCCGTGTGCG CCGCTTCCAG CAAGACGATG CGCACATCTT CTGCCGCATG GACCAGATCA CAGATGAGAT GGAGGGCCAG ATGCAGTTCC TCAGCGATGT TTACGGCATT CTGGGTTTCA AGTTCTATTT TTACCACTCT ACCCGCCCCG CAAACAAGCT CGGCTCTGAC GCGCTCTGGG ACCAGTCGGA GTCATATCTG CGCACCGCCC TCAATCGGTT CTGCGGCATC CCAGAGGAAC TACCTGACCC GTTGCACCCA GACCAGCGCT TTCACTACGA CGGCAAGCCA GACTCGGTGA AAAAGATGAA GACACTCATC AAGAAAGCGG AGCGCAGCGA TGACCCGAAT GTGTGGAAGG GCCCGACCAA CAGCGGCGAT GGCTGGATTG AGAACGCTGG TGATGGCGCC TTCTACGGCC CAAAGATTGA CATCGTTGTC GAGGACGCGC TGCGCCGTCG CCACCAGTGT GCCACGATTC AGTTGGACTT CAATCTGCCC AATCGTTTTG GTCTCAAGTA CACCCTCCCC TCGGCGGAGA AGGACGATGC GACGGTAGCC CCAACGGAGA AGCGGAGACA TGCCGACCCT GCCCCAACCG AGTCATCGAC AGCTGAGGTA GAGGGCGAGA AAAAGCCGAA GCCTGGCACG TATGAGGCTG CTGTGCGTGA CCTTGGCATT GACCTCGAGC TGGACGCCAA CCAGGCACGC CCCGTCATGA TCCACCGCGC CATTCTCGGC TCCTTGGAGC GCTCGATCGC CATTCTGTGC GAGCACTTTG GCGGTGACTG GCCGTTCTGG CTGAGCCCCC GCCAAGTGAT GGTGGTGCCT GTTTCGGCCT CCAACTACGA GTATTCACAG CATGTTCGCG ACACAATGCA CGATGCCGGT TTTTACGCGG ACGTTGACAA CGGTGCCTCC ACGTTGGACA AGAAGATCCG CAACGCGGAG AAGGCGCGCT ACAACTTCAT CCTCGTCGTT GGCCAACAGG AGCAGGACGC GACCGGCGTC AACATCCGTG CGCGCGGGGA GAAGAGGCTG GGTAACAAGT CACTCGAAGA GGCAGTGCAG TGGTTCAAGG AGTTGACGGA CACACATAAC CGGGAGTACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT