LetaA.10201.a

Methionyl-tRNA synthetase, putative

CENTER ID: LetaA.10201.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.10201.a.B1.GE42556 full length 1 747
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP21.0830

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKFFTEKAN HQALKAVLCA IFVKKPLEVI LGSTYSTPYL QLPKSRALLY SCNEAARLLW NTHAVPSPAD NSLEGEAEWL EWESTVLTPA LTPLYTQRRI TTEAELALKK LDSTIEEHKG TVAVKGSPSS KSFLVDGVLF ASLLPALCEG GLLPQAQAEG VPNLVKWFQA FQAEHAELIA SAFDVLSVQE CGDFFRVLQT YEVSPKKQKV FFATTPIYYV NASPHIGHVY STLIVDVLGR YHRVKGEEVF VMTGTDEHGQ KVAEAAAKQG ISPMDFTTSV SNEFKQCFKE MNYDMNYFIR TTNPTHEKLV QDIWKRLEAK GDIYLGKYEG WYSVSDESFL TAQNVTDGVD KEGKPCKVSL ESGHVVTWVE EENYMFRLSA FRERLLKYFH DHPNCIVPEF RRREVIKTVE KGLFDLSISR KRESVLNWSI PVPGDERHCI YVWLDALFNY YTGALTRVTA DGTETLDEEH QTLNRWPADV HVVGKDILKF HAIYWPAFLM SAELPLPERL VSHGWWTKDH RKISKSLGNA FDPVEKANEF GIDALKYFLM RESNFQDDGD YSDKNMVARL NGELADTLGN LVSRCVAPKI NVKGVWPEPA EYNDGDKTLI ALLNNLAGTV DHYYCLPDIQ HALIAIFDVL RSLNAYVTEN APWKLVKTDT ARLGTVLYVT MEGLRICTMF LQPVMPQKAK EIMDALGVPE AERIGMENYG FGIVKPGTKI GGLAEGQVIF QKVTLSAEEG ECGSKRQ
NT Sequence
ATGCTCAAGT TTTTTACAGA GAAGGCGAAT CACCAGGCCC TCAAGGCCGT GCTCTGCGCT ATATTTGTGA AGAAGCCTCT GGAGGTGATT CTGGGCAGCA CCTATAGTAC GCCGTACCTT CAGCTGCCGA AGTCGAGGGC GCTTTTGTAC AGCTGCAATG AAGCGGCGCG TCTTCTCTGG AATACGCACG CCGTGCCGTC GCCTGCCGAC AACTCACTTG AAGGAGAAGC GGAGTGGCTC GAGTGGGAGT CGACGGTGCT GACGCCAGCC TTGACCCCCC TCTACACACA GCGACGCATC ACAACCGAGG CTGAGCTTGC ACTGAAGAAG CTTGACTCCA CCATAGAGGA GCACAAGGGC ACCGTGGCTG TGAAGGGGTC CCCGTCTTCC AAGTCTTTCC TGGTCGACGG TGTCCTCTTC GCATCCCTGC TGCCTGCTCT GTGCGAAGGT GGGCTTCTCC CCCAAGCGCA GGCGGAGGGG GTGCCAAATT TGGTGAAGTG GTTCCAGGCT TTCCAGGCCG AGCACGCGGA GTTGATTGCC TCTGCTTTTG ACGTGCTGTC GGTGCAGGAG TGCGGCGACT TCTTTCGGGT TCTGCAGACG TACGAGGTGA GTCCCAAGAA GCAGAAGGTC TTCTTCGCCA CGACACCGAT CTACTACGTG AACGCGTCCC CACACATCGG CCACGTCTAC AGCACGCTCA TCGTCGACGT TCTCGGTCGC TATCACCGAG TAAAGGGAGA GGAGGTGTTT GTGATGACAG GCACGGACGA GCACGGCCAG AAGGTGGCTG AGGCAGCGGC GAAGCAGGGG ATATCGCCGA TGGACTTCAC AACGAGTGTG TCGAATGAGT TCAAGCAGTG CTTCAAGGAG ATGAATTATG ACATGAACTA CTTCATACGC ACGACGAACC CGACGCATGA GAAGCTGGTG CAGGACATAT GGAAGAGGCT GGAGGCGAAG GGTGACATCT ACCTCGGCAA GTATGAGGGC TGGTACTCGG TGAGCGACGA GTCATTTCTG ACGGCGCAAA ACGTGACGGA CGGTGTAGAC AAGGAGGGAA AGCCGTGCAA GGTCAGCCTG GAGAGCGGTC ACGTGGTGAC GTGGGTGGAG GAGGAGAACT ACATGTTCCG TCTTAGCGCG TTCCGCGAGC GACTCCTGAA GTACTTCCAT GACCACCCCA ACTGCATCGT CCCGGAGTTC CGTCGCCGCG AGGTGATCAA GACGGTCGAG AAGGGTCTTT TCGACTTATC CATCTCCCGA AAGCGCGAGT CCGTCTTGAA CTGGTCCATT CCAGTCCCCG GTGATGAGCG GCACTGCATC TACGTGTGGC TGGATGCTCT CTTCAACTAC TACACTGGCG CCCTCACCCG TGTCACGGCC GACGGCACCG AGACATTGGA CGAGGAACAC CAGACACTTA ACCGGTGGCC CGCCGACGTG CATGTTGTCG GCAAGGACAT CCTCAAGTTC CACGCCATTT ACTGGCCCGC TTTCCTGATG TCTGCGGAGC TGCCTCTGCC AGAGCGGCTG GTGTCGCATG GCTGGTGGAC GAAAGACCAC AGGAAGATCA GCAAGTCGCT CGGCAACGCG TTCGACCCGG TGGAAAAGGC AAACGAGTTT GGCATCGACG CGCTCAAATA CTTCCTGATG CGCGAGTCGA ACTTCCAGGA TGATGGTGAT TACAGTGACA AGAACATGGT AGCCCGCCTG AATGGAGAAC TCGCCGACAC ACTTGGCAAT TTGGTGTCGC GCTGTGTGGC GCCGAAGATC AACGTGAAGG GTGTGTGGCC GGAGCCTGCA GAGTACAACG ACGGCGACAA GACGCTGATC GCCTTGCTCA ATAACCTGGC TGGCACGGTG GATCACTATT ACTGCTTGCC TGACATCCAG CACGCGTTGA TTGCTATCTT TGACGTGCTC CGCAGCCTCA ACGCCTATGT GACCGAGAAC GCGCCATGGA AGCTGGTGAA GACGGACACG GCGCGCCTCG GCACAGTGCT GTACGTAACG ATGGAGGGGC TACGCATATG CACCATGTTC CTGCAGCCAG TGATGCCGCA GAAGGCGAAG GAAATTATGG ACGCGCTCGG GGTGCCGGAG GCCGAGCGCA TAGGCATGGA AAACTACGGA TTCGGTATTG TGAAGCCGGG GACGAAGATT GGTGGGTTGG CGGAAGGCCA GGTCATCTTC CAGAAGGTGA CACTCTCGGC CGAGGAGGGG GAGTGCGGTT CCAAACGACA GTAA
Details for LetaA.10201.a.B1.GE42556
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML KFFTEKANHQ ALKAVLCAIF VKKPLEVILG STYSTPYLQL PKSRALLYSC NEAARLLWNT HAVPSPADNS LEGEAEWLEW ESTVLTPALT PLYTQRRITT EAELALKKLD STIEEHKGTV AVKGSPSSKS FLVDGVLFAS LLPALCEGGL LPQAQAEGVP NLVKWFQAFQ AEHAELIASA FDVLSVQECG DFFRVLQTYE VSPKKQKVFF ATTPIYYVNA SPHIGHVYST LIVDVLGRYH RVKGEEVFVM TGTDEHGQKV AEAAAKQGIS PMDFTTSVSN EFKQCFKEMN YDMNYFIRTT NPTHEKLVQD IWKRLEAKGD IYLGKYEGWY SVSDESFLTA QNVTDGVDKE GKPCKVSLES GHVVTWVEEE NYMFRLSAFR ERLLKYFHDH PNCIVPEFRR REVIKTVEKG LFDLSISRKR ESVLNWSIPV PGDERHCIYV WLDALFNYYT GALTRVTADG TETLDEEHQT LNRWPADVHV VGKDILKFHA IYWPAFLMSA ELPLPERLVS HGWWTKDHRK ISKSLGNAFD PVEKANEFGI DALKYFLMRE SNFQDDGDYS DKNMVARLNG ELADTLGNLV SRCVAPKINV KGVWPEPAEY NDGDKTLIAL LNNLAGTVDH YYCLPDIQHA LIAIFDVLRS LNAYVTENAP WKLVKTDTAR LGTVLYVTME GLRICTMFLQ PVMPQKAKEI MDALGVPEAE RIGMENYGFG IVKPGTKIGG LAEGQVIFQK VTLSAEEGEC GSKRQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca agttttttac agagaaggcg aatcaccagg ccctcaaggc cgtgctctgc gctatatttg tgaagaagcc tctggaggtg attctgggca gcacctatag tacgccgtac cttcagctgc cgaagtcgag ggcgcttttg tacagctgca atgaagcggc gcgtcttctc tggaatacgc acgccgtgcc gtcgcctgcc gacaactcac ttgaaggaga agcggagtgg ctcgagtggg agtcgacggt gctgacgcca gccttgaccc ccctctacac acagcgacgc atcacaaccg aggctgagct tgcactgaag aagcttgact ccaccataga ggagcacaag ggcaccgtgg ctgtgaaggg gtccccgtct tccaagtctt tcctggtcga cggtgtcctc ttcgcatccc tgctgcctgc tctgtgcgaa ggtgggcttc tcccccaagc gcaggcggag ggggtgccaa atttggtgaa gtggttccag gctttccagg ccgagcacgc ggagttgatt gcctctgctt ttgacgtgct gtcggtgcag gagtgcggcg acttctttcg ggttctgcag acgtacgagg tgagtcccaa gaagcagaag gtcttcttcg ccacgacacc gatctactac gtgaacgcgt ccccacacat cggccacgtc tacagcacgc tcatcgtcga cgttctcggt cgctatcacc gagtaaaggg agaggaggtg tttgtgatga caggcacgga cgagcacggc cagaaggtgg ctgaggcagc ggcgaagcag gggatatcgc cgatggactt cacaacgagt gtgtcgaatg agttcaagca gtgcttcaag gagatgaatt atgacatgaa ctacttcata cgcacgacga acccgacgca tgagaagctg gtgcaggaca tatggaagag gctggaggcg aagggtgaca tctacctcgg caagtatgag ggctggtact cggtgagcga cgagtcattt ctgacggcgc aaaacgtgac ggacggtgta gacaaggagg gaaagccgtg caaggtcagc ctggagagcg gtcacgtggt gacgtgggtg gaggaggaga actacatgtt ccgtcttagc gcgttccgcg agcgactcct gaagtacttc catgaccacc ccaactgcat cgtcccggag ttccgtcgcc gcgaggtgat caagacggtc gagaagggtc ttttcgactt atccatctcc cgaaagcgcg agtccgtctt gaactggtcc attccagtcc ccggtgatga gcggcactgc atctacgtgt ggctggatgc tctcttcaac tactacactg gcgccctcac ccgtgtcacg gccgacggca ccgagacatt ggacgaggaa caccagacac ttaaccggtg gcccgccgac gtgcatgttg tcggcaagga catcctcaag ttccacgcca tttactggcc cgctttcctg atgtctgcgg agctgcctct gccagagcgg ctggtgtcgc atggctggtg gacgaaagac cacaggaaga tcagcaagtc gctcggcaac gcgttcgacc cggtggaaaa ggcaaacgag tttggcatcg acgcgctcaa atacttcctg atgcgcgagt cgaacttcca ggatgatggt gattacagtg acaagaacat ggtagcccgc ctgaatggag aactcgccga cacacttggc aatttggtgt cgcgctgtgt ggcgccgaag atcaacgtga agggtgtgtg gccggagcct gcagagtaca acgacggcga caagacgctg atcgccttgc tcaataacct ggctggcacg gtggatcact attactgctt gcctgacatc cagcacgcgt tgattgctat ctttgacgtg ctccgcagcc tcaacgccta tgtgaccgag aacgcgccat ggaagctggt gaagacggac acggcgcgcc tcggcacagt gctgtacgta acgatggagg ggctacgcat atgcaccatg ttcctgcagc cagtgatgcc gcagaaggcg aaggaaatta tggacgcgct cggggtgccg gaggccgagc gcataggcat ggaaaactac ggattcggta ttgtgaagcc ggggacgaag attggtgggt tggcggaagg ccaggtcatc ttccagaagg tgacactctc ggccgaggag ggggagtgcg gttccaaacg acagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat