LetaA.01593.a

Mitogen activated protein kinase, putative;protein kinase, putative

CENTER ID: LetaA.01593.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.01593.a.HZ2.GE43735 kinase domain (Jensen) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP27.0100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKYEILAQI GDGTFGSVAK AVSKKTGQLV AIKKMKQKFY TWEECVKLPE VDVVRRIHGH PNVVKLREVI RENNELYFVF EHMDGDLLGV IKKAKEKGSP PTGASPSAPL IPYPLVKNYM RQMLQALVYI HKRGYFHRDM KPENLLIRKE ASGDEVLKLA DFGLVKEIRA RPPFTDYVST RWYRAPELLL QDRFYGAAVD VWAAGCIMAE LITMRPLFPG TNEVDQLFKI MSVLGSPTEE VWAGGLRLAK KIRYNFPKVA GSGLAQALPS HIPSPALDLL RQMLVYDPKV RLTAEQCLQH PFFNVGIDEC NAPSAAALDQ LALMAKRMLP GSKTAPPALK SPTMDKVAAP KAVRAPSDAS LAETSRKFYA LGTLKDSAPS LPKLPAALST SPPSLDALQN ASSPLRGLGV LSPTGLPVVA NGHSSPNRAA TVTKGKRRSQ ETSDTLLSKK LAGLKNVPTG KGRLPMSYAL PAASAVHSPT AAPNLKVSAS MARPSPPAPT TNTRDVMGSP RHRNSYKEAE TSSPPVASAA AKVDVDLDEL MEEFASEMTA MGVLAQRHNS LPSSELKNES YVSQPALPRD PVATLLNNTR YRKKSTTSTD PTFKGSSSPR STHQLDTKKV DEAAGKSDLP RRDHLTGKGM SPTLKALLAK HKAGGLAFP
NT Sequence
ATGGACAAGT ATGAGATTCT CGCTCAAATT GGCGATGGCA CGTTCGGCTC CGTCGCAAAG GCCGTGAGCA AGAAAACCGG CCAACTTGTA GCCATCAAGA AGATGAAGCA GAAATTCTAC ACGTGGGAGG AGTGCGTCAA GCTTCCCGAG GTGGATGTCG TGCGGCGAAT TCACGGTCAC CCGAACGTGG TGAAGCTGCG CGAGGTAATT CGTGAGAACA ACGAGCTGTA CTTTGTCTTC GAGCACATGG ACGGCGATTT GCTCGGCGTC ATCAAGAAAG CAAAGGAGAA GGGATCGCCG CCCACCGGTG CATCACCCTC TGCACCGTTG ATTCCGTACC CACTCGTAAA GAACTACATG CGCCAAATGC TACAGGCACT TGTGTACATC CACAAGCGCG GGTACTTTCA CCGAGACATG AAGCCGGAAA ACCTTCTCAT TCGGAAAGAG GCGAGCGGTG ACGAGGTGCT GAAGCTGGCA GACTTCGGCC TAGTCAAGGA AATTCGTGCT CGCCCCCCGT TCACAGACTA CGTTTCAACG CGCTGGTACC GCGCACCAGA GCTGCTCCTG CAAGATCGCT TCTACGGTGC TGCGGTAGAT GTCTGGGCTG CTGGGTGCAT TATGGCGGAA CTCATTACCA TGCGGCCACT TTTTCCAGGC ACAAACGAGG TAGATCAGCT TTTCAAGATA ATGTCGGTGC TTGGGTCCCC CACTGAGGAG GTGTGGGCCG GTGGACTTCG CTTGGCCAAA AAAATCCGCT ACAACTTTCC TAAAGTAGCC GGCTCCGGCC TTGCTCAAGC GCTGCCATCG CATATCCCAT CGCCGGCGCT GGACTTGCTG CGTCAGATGC TTGTCTACGA CCCGAAAGTA AGACTGACGG CGGAGCAATG CCTGCAGCAC CCGTTCTTCA ACGTCGGTAT TGACGAGTGC AACGCGCCAT CCGCCGCCGC GCTGGATCAG CTGGCGCTCA TGGCGAAGCG GATGCTACCA GGGTCGAAAA CAGCGCCACC GGCGCTCAAG AGCCCCACCA TGGACAAGGT GGCGGCACCG AAGGCTGTTA GGGCCCCCAG CGACGCGTCA CTCGCGGAAA CCTCACGCAA GTTCTACGCA CTCGGCACTC TCAAGGACTC CGCCCCGTCG TTACCAAAGC TCCCGGCCGC GCTGTCTACC TCTCCACCAT CGCTTGACGC GCTGCAGAAC GCTTCGTCAC CTCTGCGTGG CCTCGGGGTC CTCTCACCAA CGGGGTTGCC AGTGGTCGCC AATGGCCACT CGTCTCCTAA CCGAGCAGCG ACGGTGACCA AGGGAAAGCG CCGCTCGCAG GAAACATCAG ACACCTTGCT TTCAAAGAAG TTGGCTGGTT TGAAAAATGT CCCGACAGGA AAAGGGAGGC TGCCCATGTC TTATGCACTT CCGGCTGCGA GCGCCGTGCA TTCGCCTACT GCAGCACCCA ACCTGAAGGT CTCCGCGTCC ATGGCGAGGC CATCCCCCCC GGCACCCACC ACCAACACCC GCGACGTCAT GGGGTCACCG CGGCACCGCA ACTCGTATAA GGAAGCTGAG ACTAGTTCCC CACCGGTGGC ATCCGCGGCG GCGAAGGTAG ATGTGGACCT GGATGAGCTC ATGGAGGAGT TTGCTTCGGA GATGACCGCC ATGGGGGTGC TGGCTCAGCG GCACAACTCG CTTCCGTCGT CTGAATTGAA GAACGAAAGC TACGTTTCTC AGCCAGCTCT TCCGCGCGAT CCTGTGGCAA CGCTGCTGAA CAACACCCGA TATCGAAAGA AATCCACTAC GAGTACCGAC CCGACGTTCA AAGGGAGCAG CTCACCCCGC TCCACACACC AGCTGGATAC AAAGAAGGTG GATGAAGCTG CCGGGAAGAG CGACCTTCCT CGCAGAGACC ACTTGACCGG GAAAGGGATG TCTCCAACGC TGAAGGCACT GCTAGCAAAG CACAAGGCAG GCGGTCTGGC GTTCCCGTAG
Details for LetaA.01593.a.HZ2.GE43735
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 55
Validated AA Sequence
MDGDLLXVIK KAKEKGSPPT GASPSAPLIP YPLVKNYMRQ MLQALVYIHK RGYFHRDMKP ENLLIRKEAS GDEVLKLADF GLVKEIRARP PFTDYVSTRW YRAPELLLQD RFYGAAVDVW AAGCIMAELI TMRPLFPGTN EVDQLFKIMS VLGSPTEEVW AGGLRLAKKI RYNFPKVAGS GLAQALPSHI PSPALDLLRQ MLVYDPKVRL TAEQCLQHPF FNVGIDECNA PSAAALDQLA LMAKRMLPGS KTA
Validated NT Sequence
tggcgctgtt ttcgaccctg gtagcatccg cttcgccatg agcgccagct gatccagcgc ggcggcggat ggcgcgttgc actcgtcaat accgacgttg aagaacgggt gctgcaggca ttgctccgcc gtcagtctta ctttcgggtc gtagacaagc atctgacgca gcaagtccag cgccggcgat gggatatgcg atggcagcgc ttgagcaagg ccggagccgg ctactttagg aaagttgtag cggatttttt tggccaagcg aagtccaccg gcccacacct cctcagtggg ggacccaagc accgacatta tcttgaaaag ctgatctacc tcgtttgtgc ctggaaaaag tggccgcatg gtaatgagtt ccgccataat gcacccagca gcccagacat ctaccgcagc accgtagaag cgatcttgca ggagcagctc tggtgcgcgg taccagcgcg ttgaaacgta gtctgtgaac ggggggcgag cacgaatttc cttgactagg ccgaagtctg ccagcttcag cacctcgtca ccgctcgcct ctttccgaat gagaaggttt tccggcttca tgtctcggtg aaagtacccg cgcttgtgga tgtacacaag tgcctgtagc atttggcgca tgtagttctt tacgagtggg tacggaatca acggngcaga gggtgatgca ccggtgggcg gcgatccctt ctcctttgct ttcttgatga cgcngagcaa atcgccgtcc atgtgctnga agacaaagna cagctcgttg ttctcnnnan ntnnnntcgc gcagcttcac cacgttcggg tgaccgtgaa ttcgccgcac gacatccacc tcgggaagct tnnacgcant cntcccnngn gtagaatntn tgnttcatct tcttgatgnn tncaaagttg gccggttttn nngctcncgg nccttttgcg acggagccga acgtgccntn nncaatttga gcgagannnt catacttgtc catngancca ggaccnnggn tnngannntn cnnnnnncca ccaatnngtt ntntnnnnnc ctcaataann tngttatcct ccangtncnn ntcttcaggg gtctgatcag cnnnaattct aatnccgtng tncaagaatn ntaaggagtc canttcctta cccngtnttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tnnnntntng aagaagatct ctgaagatcc atcggacacc nnnnaantga tgtgagtctc aggcttgact tctgncnngn cctctggctt agcttcttga ttgacttctg agtccgactt catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD KYEILAQIGD GTFGSVAKAV SKKTGQLVAI KKMKQKFYTW EECVKLPEVD VVRRIHGHPN VVKLREVIRE NNELYFVFEH MDGDLLGVIK KAKEKGSPPT GASPSAPLIP YPLVKNYMRQ MLQALVYIHK RGYFHRDMKP ENLLIRKEAS GDEVLKLADF GLVKEIRARP PFTDYVSTRW YRAPELLLQD RFYGAAVDVW AAGCIMAELI TMRPLFPGTN EVDQLFKIMS VLGSPTEEVW AGGLRLAKKI RYNFPKVAGS GLAQALPSHI PSPALDLLRQ MLVYDPKVRL TAEQCLQHPF FNVGIDECNA PSAAALDQLA LMAKRMLPGS KTAPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggacaagta tgagattctc gctcaaattg gcgatggcac gttcggctcc gtcgcaaagg ccgtgagcaa gaaaaccggc caacttgtag ccatcaagaa gatgaagcag aaattctaca cgtgggagga gtgcgtcaag cttcccgagg tggatgtcgt gcggcgaatt cacggtcacc cgaacgtggt gaagctgcgc gaggtaattc gtgagaacaa cgagctgtac tttgtcttcg agcacatgga cggcgatttg ctcggcgtca tcaagaaagc aaaggagaag ggatcgccgc ccaccggtgc atcaccctct gcaccgttga ttccgtaccc actcgtaaag aactacatgc gccaaatgct acaggcactt gtgtacatcc acaagcgcgg gtactttcac cgagacatga agccggaaaa ccttctcatt cggaaagagg cgagcggtga cgaggtgctg aagctggcag acttcggcct agtcaaggaa attcgtgctc gccccccgtt cacagactac gtttcaacgc gctggtaccg cgcaccagag ctgctcctgc aagatcgctt ctacggtgct gcggtagatg tctgggctgc tgggtgcatt atggcggaac tcattaccat gcggccactt tttccaggca caaacgaggt agatcagctt ttcaagataa tgtcggtgct tgggtccccc actgaggagg tgtgggccgg tggacttcgc ttggccaaaa aaatccgcta caactttcct aaagtagccg gctccggcct tgctcaagcg ctgccatcgc atatcccatc gccggcgctg gacttgctgc gtcagatgct tgtctacgac ccgaaagtaa gactgacggc ggagcaatgc ctgcagcacc cgttcttcaa cgtcggtatt gacgagtgca acgcgccatc cgccgccgcg ctggatcagc tggcgctcat ggcgaagcgg atgctaccag ggtcgaaaac agcgccaccg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa